Revision as of 20:37, 19 October 2009

The Encapsulator

A key challenge in metabolic engineering is to improve productivity and yield. Potential applications range from the production of valuable compounds such as therapeutic molecules and biofuels to the degradation of toxic wastes. There is increasing recognition that spatial organization can play an important role in optimizing pathway efficiency. Specifically, the spatial co-localization of consecutive enzymes in a pathway can result in efficient translocation of substrates between enzymes, an effect known as enzyme "channeling". Here we report the design, modeling and construction of a bacterial micro-organelle based system for the targeted co-localization of selected enzymes. Our "Encapsulator" represents a fundamentally new class of parts which, in nature consist of metabolic enzymes encased within a multi-protein shell reminiscent of a viral capsid. Micro-compartments based on encapsulin (and similar proteins) represent an experimentally amenable system to investigate the effects of channeling in potential downstream applications.

Sponsored by:

The Parkinson Lab at the Hospital for Sick Children, Toronto	Center for Analysis of Genome Evolution and Function	University of Toronto Engineering Society	Skule-alumni-small.jpg Engineering Society Alumni
Collaborative Graduate Program in Genome Biology and Bioinformatics

We are proud to support our colleagues in Valencia!

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 =The Encapsulator=