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Team:Illinois/Notebook
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| - | == ''' | + | == '''Post-Summer Progress''' == |
| - | + | This section includes entries for lab work done after the summer was over. | |
| + | |||
| + | == '''September 24''' == | ||
| + | |||
| + | PCRed all target sequences w/ BB sRNAs w/ no results | ||
| + | |||
| + | == '''September 25''' == | ||
| + | |||
| + | PCRed sRNA genes (MicC, MicA, MicF) and their respective target sequences (OmpC, OmpA, OmpF). The gel was run with the Vogel primers as a control since the previous attempt to PCR Biobricks failed. | ||
| + | |||
| + | Also PCRed plasmid pSB1A2. Used 56°C for annealing temperature instead of 58°C and got better results. | ||
| + | |||
| + | Also restreaked plates of E. coli containing plasmid PJU-334 and Biobricks BBa_K091101, BBa_I0500, and BBa_R0040. | ||
| + | |||
| + | == '''September 26''' == | ||
| + | |||
| + | PCRed sRNA target sequences OmpC, OmpA, OmpF, FtsZ, PtsG, GalK,. For PCRs, used | ||
| + | *4 μL chromosomal template | ||
| + | *0.4 μL of each primer | ||
| + | *5 μL 10x buffer | ||
| + | *0.8 μL Pfu polymerase | ||
| + | *1 μL dNTP mix | ||
| + | *38.4 μL dH<sub>2</sub>O | ||
| + | |||
| + | Also digested OmpC, OmpA, and OmpF target sequences from yesterday's PCR using | ||
| + | *1 μL EcoRI-HF (20,000 U/mL) | ||
| + | *3.5 μL 10x Buffer 4 | ||
| + | *30 μL DNA | ||
| + | *0.35 μL BSA | ||
| + | *0.15 μL dH<sub>2</sub>O | ||
| + | |||
| + | Also cultured cells containing plasmid PJU-334 and Biobricks BBa_K091101, BBa_I0500, and BBa_R0040 overnight. | ||
| + | |||
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{{IllinoisBottomNav}} | {{IllinoisBottomNav}} | ||
Revision as of 21:15, 19 October 2009
Contents |
Notebook
This page will include periodic entries during our research. We will be documenting the progression of our project, any problems or obstacles encountered, and solutions to problems.
Browse by Lab Team
Click on a group name below to view notebook entries and information pertaining to one of our individual lab teams.
Post-Summer Progress
This section includes entries for lab work done after the summer was over.
September 24
PCRed all target sequences w/ BB sRNAs w/ no results
September 25
PCRed sRNA genes (MicC, MicA, MicF) and their respective target sequences (OmpC, OmpA, OmpF). The gel was run with the Vogel primers as a control since the previous attempt to PCR Biobricks failed.
Also PCRed plasmid pSB1A2. Used 56°C for annealing temperature instead of 58°C and got better results.
Also restreaked plates of E. coli containing plasmid PJU-334 and Biobricks BBa_K091101, BBa_I0500, and BBa_R0040.
September 26
PCRed sRNA target sequences OmpC, OmpA, OmpF, FtsZ, PtsG, GalK,. For PCRs, used
- 4 μL chromosomal template
- 0.4 μL of each primer
- 5 μL 10x buffer
- 0.8 μL Pfu polymerase
- 1 μL dNTP mix
- 38.4 μL dH2O
Also digested OmpC, OmpA, and OmpF target sequences from yesterday's PCR using
- 1 μL EcoRI-HF (20,000 U/mL)
- 3.5 μL 10x Buffer 4
- 30 μL DNA
- 0.35 μL BSA
- 0.15 μL dH2O
Also cultured cells containing plasmid PJU-334 and Biobricks BBa_K091101, BBa_I0500, and BBa_R0040 overnight.
