Team:Bologna/T-REx Story

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Revision as of 22:44, 19 October 2009

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Story of a T-REX

(Trans-Repressor of Expression)

The main goal of our project is the realization of a device for post-transcriptional regulation of gene expression in Escherichia coli, in order to switch protein production of a gene of interest.

The Parts

To achieve this aim we designed, using BASER tool, two complementary 50bp non-coding sequences:

CIS-repressing, to be assembled upstream of the target protein coding sequence; it contains a ribosomal binding site (RBS):

5' Prefix - AACACAAACTATCACTTTAACAACACATTACATATACATTAAAATATTACAAAGAGGAGAAA - 3' Suffix

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 <font face="Calibri" font size="8" color="#000000"><b>Story of a T-REX<br><br></b></font>
 <font face="Calibri" font size="5" color="#000000">(<b>T</b>rans-<b>R</b>epressor of <b>Ex</b>pression)</font></center>
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-To achieve this aim we designed, using BASER tool, two complementary <b>50bp non-coding sequences:</b>
+To achieve this aim we designed, using <a href="https://2009.igem.org/Team:Bologna/Software">BASER</a> tool, two complementary <b>50bp non-coding sequences:</b><br><br>
+<ul>
+<li><font color="#5f9ea0"><b><u>CIS-repressing</b></u></font>, to be assembled upstream of the target protein coding sequence; it contains a ribosomal binding site <font color="#87ceeb"><b>(RBS)</b></font>:
+</ul>
+<br>
+' <i>Prefix</i> - <font size="3" color="#5f9ea0"><b>AACACAAACTATCACTTTAACAACACATTACATATACATTAAAATATTAC</b></font><font size="3" color="#87ceeb"><b>AAAGAGGAGAAA</b></font> - 3' <i>Suffix</i>
+</font>
+</font>
+</font>
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