Template:Team:KULeuven/10 October 2009/VanillinProduction

From 2009.igem.org

(Difference between revisions)
(New page: * Vanilla experiment take 2 ** From the grown cultures (EFT, PEFT, PAMSCOT, SAMS) transfer 1:100 to a new LB medium ** Add 50 mg/L Tyrosine to 1 falcon and 5 mg/L to another test tube ** ...)
 
Line 1: Line 1:
* Vanilla experiment take 2
* Vanilla experiment take 2
** From the grown cultures (EFT, PEFT, PAMSCOT, SAMS) transfer 1:100 to a new LB medium
** From the grown cultures (EFT, PEFT, PAMSCOT, SAMS) transfer 1:100 to a new LB medium
-
** Add 50 mg/L Tyrosine to 1 falcon and 5 mg/L to another test tube
+
** Add 50 mg/L Tyrosine to 1 falcon and 5 mg/L to another test tube
** Let the PEFT and EFT cells grow for 2 hours, then add 50 mg/L Ferrulic acid and 5 mg/L ferrulic acid
** Let the PEFT and EFT cells grow for 2 hours, then add 50 mg/L Ferrulic acid and 5 mg/L ferrulic acid
*** Note: Ferrulic accid is dissolved in alcohol, 10 mg/ml
*** Note: Ferrulic accid is dissolved in alcohol, 10 mg/ml

Latest revision as of 23:19, 19 October 2009

  • Vanilla experiment take 2
    • From the grown cultures (EFT, PEFT, PAMSCOT, SAMS) transfer 1:100 to a new LB medium
    • Add 50 mg/L Tyrosine to 1 falcon and 5 mg/L to another test tube
    • Let the PEFT and EFT cells grow for 2 hours, then add 50 mg/L Ferrulic acid and 5 mg/L ferrulic acid
      • Note: Ferrulic accid is dissolved in alcohol, 10 mg/ml
    • Take samples for PAMSCOT and SAMS at: 0, 0', 30, 60, 120, 180, 240, 300 and 360 minutes
    • Take samples for PEFT and EFT (after 2 hours growth) at: 0, 0', 20, 40, 60, 90, 120, 180 and 240 minutes
    • Measure the OD at 595 nm

for 50 mg/μl Ferrulic acid

part 0 0' 20 40 60 90 120 180 240
PEFT 0,113 0,117 0,147 0,210 0,317 0,468 0,623 0,845 0,927
EFT 0,111 0,250 0,794

for 5 mg/μl Ferrulic acid

part 0 0' 20 40 60 90 120 180 240
PEFT 0,117 0,122 0,156 0,205 0,332 0,430 0,632 0,860 0,950
EFT 0,106 0,259 0,791

for 50 mg/μl Tyrosine

part 0' 30 60 120 180 240 300 360
PAMSCOT 0,064 0,020 0,022 0,062 0,101 0,202 0,370 0,499
SAMS 0,032 0,351 1,008

for 5 mg/μl Tyrosine

part 0' 30 60 120 180 240 300 360
PAMSCOT 0,028 0,021 0,026 0,067 0,143 0,313 0,559
SAMS 0,030 0,317 0,996
Retrieved from "http://2009.igem.org/Template:Team:KULeuven/10_October_2009/VanillinProduction"