"
Parts
From 2009.igem.org
| Line 3: | Line 3: | ||
|[[Home]]|[[Project]]|[[Team]] |[[Protocols]] ||bgcolor="yellow"| ''' [[Parts]]'''|| [[Modeling]] |[[Gallery]] |[[Notebook]]|[[Ethics]]| | |[[Home]]|[[Project]]|[[Team]] |[[Protocols]] ||bgcolor="yellow"| ''' [[Parts]]'''|| [[Modeling]] |[[Gallery]] |[[Notebook]]|[[Ethics]]| | ||
|} | |} | ||
| + | === Parts === | ||
| + | '''PDC''' | ||
| + | *Primary nucleaic acid sequence | ||
| + | ATGAGCGAAATCACCCTGGGCAAATACCTGTTTGAACGCCTGAAACAGGTGAACGTGAACACCGTGTTTGGCCTGCCTGGCGATTTTAATCTGAGCCTGCTGGATAAAATCTACGAAGTGGAAGGCATGCGCTGGGCTGGCAACGCCAACGAACTGAACGCTGCCTACGCTGCCGATGGCTACGCTCGCATCAAAGGCATGAGCTGTATCATCACGACCTTTGGCGTGGGCGAACTGAGCGCTCTGAACGGCATTGCTGGCAGCTACGCCGAACACGTGGGCGTGCTGCATGTGGTGGGCGTGCCCAGCATTAGCGCTCAGGCCAAACAGCTGCTGCTGCACCATACCCTGGGCAACGGCGATTTTACCGTGTTTCACCGCATGAGCGCCAACATCAGCGAAACCACCGCCATGATCACCGATATCGCCACCGCTCCCGCTGAAATCGATCGCTGCATCCGCACCACCTACGTGACCCAACGCCCTGTGTATCTGGGCCTCCCTGCCAACCTGGTGGATCTGAACGTGCCTGCCAAACTGCTGCAGACCCCCATTGATATGAGCCTGAAACCCAACGATGCCGAAAGCGAAAAAGAAGTGATCGATACCATCCTGGCCCTGGTGAAAGATGCCAAAAACCCCGTGATCCTGGCCGATGCCTGCTGCAGTCGCCACGATGTGAAAGCCGAAACCAAAAAACTGATCGATCTGACCCAGTTTCCCGCTTTTGTGACCCCCATGGGCAAAGGCAGCATCGATGAACAGCACCCTCGCTACGGTGGCGTGTATGTGGGCACCCTGAGCAAACCCGAAGTGAAAGAAGCCGTGGAAAGCGCTGATCTGATCCTGAGCGTGGGTGCCCTGCTGTCGGATTTTAACACCGGCAGCTTTAGCTACAGCTACAAGACCAAAAACATCGTGGAATTTCACAGCGATCACATGAAAATCCGCAACGCCACCTTTCCTGGCGTGCAGATGAAATTTGTGCTGCAGAAACTGCTGACCACCATTGCCGATGCCGCTAAAGGCTACAAACCCGTGGCTGTTCCCGCTCGCACCCCTGCTAATGCTGCTGTGCCTGCCAGCACCCCTCTGAAACAAGAATGGATGTGGAACCAGCTGGGCAACTTTCTGCAAGAGGGTGATGTGGTGATCGCCGAAACCGGCACCAGCGCCTTTGGCATCAACCAGACCACCTTTCCCAACAACACCTACGGCATCAGCCAGGTGCTGTGGGGCAGCATCGGCTTTACGACCGGTGCTACCCTGGGTGCTGCGTTTGCCGCTGAAGAAATCGATCCCAAAAAACGCGTGATTCTGTTTATCGGTGATGGCAGCCTGCAGCTGACCGTGCAAGAAATCAGCACCATGATCCGCTGGGGTCTGAAACCCTACCTGTTTGTGCTGAATAACGATGGCTACACCATCGAAAAACTGATCCACGGTCCCAAAGCCCAGTACAACGAAATCCAGGGCTGGGATCACCTGAGTCTGCTGCCGACCTTTGGCGCTAAAGATTACGAAACCCATCGCGTGGCTACCACGGGTGAATGGGATAAACTGACCCAGGATAAGAGCTTTAACGATAACAGCAAGATCCGCATGATCGAAATCATGCTGCCCGTGTTTGATGCTCCCCAGAACCTGGTGGAACAGGCCAAACTGACCGCTGCCACCAACGCCAAACAGTAA | ||
| + | |||
| + | *Description of function | ||
| + | PDC is short for pyruvate decarboxylase, it is a homotetrameric enzyme Pyruvate decarboxylase which can converting pyruvate into acetaldehyde and carbon dioxide. | ||
| + | |||
| + | *Acknowedgment of sources and references | ||
| + | Kellermann,E., Seeboth,P.G. and Hollenberg,C.P. | ||
| + | Analysis of the primary structure and promoter function of a pyruvate decarboxylase gene (PDC1) from Saccharomyces cerevisiae, Nucleic Acids Res. 14 (22), 8963-8977 (1986) | ||
| + | |||
| + | |||
| + | '''ADH''' | ||
| + | *Primary nucleaic acid sequence | ||
| + | ATGTCGATCCCCGAAACCCAGAAAGGCGTGATCTTTTACGAAAGCCACGGCAAACTGGAATACAAAGATATCCCCGTGCCCAAACCCAAAGCCAATGAACTGCTGATCAACGTGAAATACAGTGGCGTGTGCCACACCGATCTGCACGCCTGGCATGGCGATTGGCCTCTGCCCGTGAAACTGCCCCTGGTGGGTGGCCATGAAGGCGCTGGCGTGGTGGTGGGCATGGGCGAAAACGTGAAAGGCTGGAAAATCGGCGATTACGCTGGCATCAAATGGCTGAACGGCAGCTGCATGGCCTGCGAATACTGCGAACTGGGCAACGAAAGCAACTGTCCCCACGCCGATCTGAGCGGCTACACCCACGATGGCAGCTTTCAGCAGTACGCCACCGCTGATGCCGTGCAGGCTGCCCACATTCCCCAGGGCACCGATCTGGCTCAGGTGGCTCCCATTCTGTGCGCTGGCATTACCGTGTACAAAGCCCTGAAAAGCGCCAACCTGATGGCTGGCCACTGGGTCGCCATTAGTGGCGCTGCTGGCGGTCTGGGCAGTCTGGCCGTGCAGTATGCCAAAGCCATGGGCTATCGCGTGCTGGGCATCGATGGTGGCGAAGGCAAAGAAGAACTGTTTCGCAGCATCGGTGGCGAAGTGTTTATCGATTTCACCAAAGAAAAAGATATCGTGGGTGCCGTCCTGAAAGCCACCGATGGCGGTGCTCACGGCGTGATCAACGTCAGCGTGAGCGAAGCCGCTATCGAAGCCAGCACCCGCTACGTTCGCGCTAATGGCACCACCGTGCTGGTCGGCATGCCTGCTGGCGCTAAATGCTGCTCGGATGTGTTTAACCAGGTGGTGAAAAGCATCAGCATCGTGGGCAGCTATGTGGGCAATCGCGCTGATACTCGCGAAGCCCTGGATTTTTTTGCTCGCGGTCTGGTGAAAAGCCCCATCAAAGTGGTGGGCCTGAGCACCCTGCCCGAAATCTACGAAAAAATGGAAAAAGGCCAGATCGTCGGTCGCTACGTGGTGGATACCAGCAAATAATAGGGATCC | ||
| + | *Description of function | ||
| + | ADH is short for Alcohol dehydrogenase which can catalyse the generation of ethanol. | ||
| + | *Acknowedgment of sources and references | ||
| + | Bennetzen JL and Hall BD(1982)The primary structure of the Saccharomyces cerevisae gene for alcohol dehydrogenase.J Biol Chem.257(6):3018-25 | ||
| + | |||
| + | |||
| + | '''groESL''' | ||
| + | *Primary nucleaic acid sequence | ||
| + | CAGTTATCAGTTATCAGTTGTCAGGTTATTTCCACTGTTCACTGTTCACTGATAACTGTTAACTGTCCACTGTTCGGAAACCCGTACACACCAAAACATTGCCATGCTAGGATGGTTTTCGGTAAATTAGCACTCAGGAGTGGAGAGTGCTAACTCTGGAGAAATTAAAT | ||
| + | *Description of function | ||
| + | heat-shock-responsive operon | ||
| + | *Acknowedgment of sources and references | ||
| + | Cloning and characterization of the major groESL operon from a nitrogen-¢xing cyanobacterium Anabaena sp. strain L-31 | ||
| + | |||
| + | |||
| + | '''Rbc''' | ||
| + | *Primary nucleaic acid sequence | ||
| + | CAGTCAATGGAGAGCATTGCCATAAGTAAAGGCATCCCCTGCGTGATAAGATTACCTTCAGAAAACAGATAGTTGCTGGGTTATCGCAGATTTTTCTCGCAACCAAATAACTGTAAATAATAACTGTCTCTGGGGCGACGGTAGGCTTTATATTGCCAAATTTCGCCCGTGGGAGAAAGCTAGGCTATTCAATGTTTATGGAGGACTGACCTAG | ||
| + | *Description of function | ||
| + | RuBisCO promoter | ||
| + | *Acknowedgment of sources and references | ||
| + | Mutating the Synechocystis sp. PCC6803 RuBisCO promoter as a means of controlling levels of gene expression in cyanobacteria | ||
Revision as of 02:03, 20 October 2009
| Home|Project|Team |Protocols | Parts | Modeling |Gallery |Notebook|Ethics| |
Parts
PDC
- Primary nucleaic acid sequence
ATGAGCGAAATCACCCTGGGCAAATACCTGTTTGAACGCCTGAAACAGGTGAACGTGAACACCGTGTTTGGCCTGCCTGGCGATTTTAATCTGAGCCTGCTGGATAAAATCTACGAAGTGGAAGGCATGCGCTGGGCTGGCAACGCCAACGAACTGAACGCTGCCTACGCTGCCGATGGCTACGCTCGCATCAAAGGCATGAGCTGTATCATCACGACCTTTGGCGTGGGCGAACTGAGCGCTCTGAACGGCATTGCTGGCAGCTACGCCGAACACGTGGGCGTGCTGCATGTGGTGGGCGTGCCCAGCATTAGCGCTCAGGCCAAACAGCTGCTGCTGCACCATACCCTGGGCAACGGCGATTTTACCGTGTTTCACCGCATGAGCGCCAACATCAGCGAAACCACCGCCATGATCACCGATATCGCCACCGCTCCCGCTGAAATCGATCGCTGCATCCGCACCACCTACGTGACCCAACGCCCTGTGTATCTGGGCCTCCCTGCCAACCTGGTGGATCTGAACGTGCCTGCCAAACTGCTGCAGACCCCCATTGATATGAGCCTGAAACCCAACGATGCCGAAAGCGAAAAAGAAGTGATCGATACCATCCTGGCCCTGGTGAAAGATGCCAAAAACCCCGTGATCCTGGCCGATGCCTGCTGCAGTCGCCACGATGTGAAAGCCGAAACCAAAAAACTGATCGATCTGACCCAGTTTCCCGCTTTTGTGACCCCCATGGGCAAAGGCAGCATCGATGAACAGCACCCTCGCTACGGTGGCGTGTATGTGGGCACCCTGAGCAAACCCGAAGTGAAAGAAGCCGTGGAAAGCGCTGATCTGATCCTGAGCGTGGGTGCCCTGCTGTCGGATTTTAACACCGGCAGCTTTAGCTACAGCTACAAGACCAAAAACATCGTGGAATTTCACAGCGATCACATGAAAATCCGCAACGCCACCTTTCCTGGCGTGCAGATGAAATTTGTGCTGCAGAAACTGCTGACCACCATTGCCGATGCCGCTAAAGGCTACAAACCCGTGGCTGTTCCCGCTCGCACCCCTGCTAATGCTGCTGTGCCTGCCAGCACCCCTCTGAAACAAGAATGGATGTGGAACCAGCTGGGCAACTTTCTGCAAGAGGGTGATGTGGTGATCGCCGAAACCGGCACCAGCGCCTTTGGCATCAACCAGACCACCTTTCCCAACAACACCTACGGCATCAGCCAGGTGCTGTGGGGCAGCATCGGCTTTACGACCGGTGCTACCCTGGGTGCTGCGTTTGCCGCTGAAGAAATCGATCCCAAAAAACGCGTGATTCTGTTTATCGGTGATGGCAGCCTGCAGCTGACCGTGCAAGAAATCAGCACCATGATCCGCTGGGGTCTGAAACCCTACCTGTTTGTGCTGAATAACGATGGCTACACCATCGAAAAACTGATCCACGGTCCCAAAGCCCAGTACAACGAAATCCAGGGCTGGGATCACCTGAGTCTGCTGCCGACCTTTGGCGCTAAAGATTACGAAACCCATCGCGTGGCTACCACGGGTGAATGGGATAAACTGACCCAGGATAAGAGCTTTAACGATAACAGCAAGATCCGCATGATCGAAATCATGCTGCCCGTGTTTGATGCTCCCCAGAACCTGGTGGAACAGGCCAAACTGACCGCTGCCACCAACGCCAAACAGTAA
- Description of function
PDC is short for pyruvate decarboxylase, it is a homotetrameric enzyme Pyruvate decarboxylase which can converting pyruvate into acetaldehyde and carbon dioxide.
- Acknowedgment of sources and references
Kellermann,E., Seeboth,P.G. and Hollenberg,C.P. Analysis of the primary structure and promoter function of a pyruvate decarboxylase gene (PDC1) from Saccharomyces cerevisiae, Nucleic Acids Res. 14 (22), 8963-8977 (1986)
ADH
- Primary nucleaic acid sequence
ATGTCGATCCCCGAAACCCAGAAAGGCGTGATCTTTTACGAAAGCCACGGCAAACTGGAATACAAAGATATCCCCGTGCCCAAACCCAAAGCCAATGAACTGCTGATCAACGTGAAATACAGTGGCGTGTGCCACACCGATCTGCACGCCTGGCATGGCGATTGGCCTCTGCCCGTGAAACTGCCCCTGGTGGGTGGCCATGAAGGCGCTGGCGTGGTGGTGGGCATGGGCGAAAACGTGAAAGGCTGGAAAATCGGCGATTACGCTGGCATCAAATGGCTGAACGGCAGCTGCATGGCCTGCGAATACTGCGAACTGGGCAACGAAAGCAACTGTCCCCACGCCGATCTGAGCGGCTACACCCACGATGGCAGCTTTCAGCAGTACGCCACCGCTGATGCCGTGCAGGCTGCCCACATTCCCCAGGGCACCGATCTGGCTCAGGTGGCTCCCATTCTGTGCGCTGGCATTACCGTGTACAAAGCCCTGAAAAGCGCCAACCTGATGGCTGGCCACTGGGTCGCCATTAGTGGCGCTGCTGGCGGTCTGGGCAGTCTGGCCGTGCAGTATGCCAAAGCCATGGGCTATCGCGTGCTGGGCATCGATGGTGGCGAAGGCAAAGAAGAACTGTTTCGCAGCATCGGTGGCGAAGTGTTTATCGATTTCACCAAAGAAAAAGATATCGTGGGTGCCGTCCTGAAAGCCACCGATGGCGGTGCTCACGGCGTGATCAACGTCAGCGTGAGCGAAGCCGCTATCGAAGCCAGCACCCGCTACGTTCGCGCTAATGGCACCACCGTGCTGGTCGGCATGCCTGCTGGCGCTAAATGCTGCTCGGATGTGTTTAACCAGGTGGTGAAAAGCATCAGCATCGTGGGCAGCTATGTGGGCAATCGCGCTGATACTCGCGAAGCCCTGGATTTTTTTGCTCGCGGTCTGGTGAAAAGCCCCATCAAAGTGGTGGGCCTGAGCACCCTGCCCGAAATCTACGAAAAAATGGAAAAAGGCCAGATCGTCGGTCGCTACGTGGTGGATACCAGCAAATAATAGGGATCC
- Description of function
ADH is short for Alcohol dehydrogenase which can catalyse the generation of ethanol.
- Acknowedgment of sources and references
Bennetzen JL and Hall BD(1982)The primary structure of the Saccharomyces cerevisae gene for alcohol dehydrogenase.J Biol Chem.257(6):3018-25
groESL
- Primary nucleaic acid sequence
CAGTTATCAGTTATCAGTTGTCAGGTTATTTCCACTGTTCACTGTTCACTGATAACTGTTAACTGTCCACTGTTCGGAAACCCGTACACACCAAAACATTGCCATGCTAGGATGGTTTTCGGTAAATTAGCACTCAGGAGTGGAGAGTGCTAACTCTGGAGAAATTAAAT
- Description of function
heat-shock-responsive operon
- Acknowedgment of sources and references
Cloning and characterization of the major groESL operon from a nitrogen-¢xing cyanobacterium Anabaena sp. strain L-31
Rbc
- Primary nucleaic acid sequence
CAGTCAATGGAGAGCATTGCCATAAGTAAAGGCATCCCCTGCGTGATAAGATTACCTTCAGAAAACAGATAGTTGCTGGGTTATCGCAGATTTTTCTCGCAACCAAATAACTGTAAATAATAACTGTCTCTGGGGCGACGGTAGGCTTTATATTGCCAAATTTCGCCCGTGGGAGAAAGCTAGGCTATTCAATGTTTATGGAGGACTGACCTAG
- Description of function
RuBisCO promoter
- Acknowedgment of sources and references
Mutating the Synechocystis sp. PCC6803 RuBisCO promoter as a means of controlling levels of gene expression in cyanobacteria
