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Parts
From 2009.igem.org
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'''PDC''' | '''PDC''' | ||
*Primary nucleaic acid sequence | *Primary nucleaic acid sequence | ||
| - | + | #ATGAGCGAAATCACCCTGGGCAAATACCTGTTTGAACGCCTGAAACAGGTGAACGTGAACACCGTGTTTGGCCTGCCTGGCGATTTTAATCTGAGCCTGCTGGATAAAATCT#ACGAAGTGGAAGGCATGCGCTGGGCTGGCAACGCCAACGAACTGAACGCTGCCTACGCTGCCGATGGCTACGCTCGCATCAAAGGCATGAGCTGTATCATCACGACCTTTGG#CGTGGGCGAACTGAGCGCTCTGAACGGCATTGCTGGCAGCTACGCCGAACACGTGGGCGTGCTGCATGTGGTGGGCGTGCCCAGCATTAGCGCTCAGGCCAAACAGCTGCTG#CTGCACCATACCCTGGGCAACGGCGATTTTACCGTGTTTCACCGCATGAGCGCCAACATCAGCGAAACCACCGCCATGATCACCGATATCGCCACCGCTCCCGCTGAAATCG#ATCGCTGCATCCGCACCACCTACGTGACCCAACGCCCTGTGTATCTGGGCCTCCCTGCCAACCTGGTGGATCTGAACGTGCCTGCCAAACTGCTGCAGACCCCCATTGATAT#GAGCCTGAAACCCAACGATGCCGAAAGCGAAAAAGAAGTGATCGATACCATCCTGGCCCTGGTGAAAGATGCCAAAAACCCCGTGATCCTGGCCGATGCCTGCTGCAGTCGC#CACGATGTGAAAGCCGAAACCAAAAAACTGATCGATCTGACCCAGTTTCCCGCTTTTGTGACCCCCATGGGCAAAGGCAGCATCGATGAACAGCACCCTCGCTACGGTGGCG#TGTATGTGGGCACCCTGAGCAAACCCGAAGTGAAAGAAGCCGTGGAAAGCGCTGATCTGATCCTGAGCGTGGGTGCCCTGCTGTCGGATTTTAACACCGGCAGCTTTAGCTA#CAGCTACAAGACCAAAAACATCGTGGAATTTCACAGCGATCACATGAAAATCCGCAACGCCACCTTTCCTGGCGTGCAGATGAAATTTGTGCTGCAGAAACTGCTGACCACC#ATTGCCGATGCCGCTAAAGGCTACAAACCCGTGGCTGTTCCCGCTCGCACCCCTGCTAATGCTGCTGTGCCTGCCAGCACCCCTCTGAAACAAGAATGGATGTGGAACCAGC#TGGGCAACTTTCTGCAAGAGGGTGATGTGGTGATCGCCGAAACCGGCACCAGCGCCTTTGGCATCAACCAGACCACCTTTCCCAACAACACCTACGGCATCAGCCAGGTGCT#GTGGGGCAGCATCGGCTTTACGACCGGTGCTACCCTGGGTGCTGCGTTTGCCGCTGAAGAAATCGATCCCAAAAAACGCGTGATTCTGTTTATCGGTGATGGCAGCCTGCAG#CTGACCGTGCAAGAAATCAGCACCATGATCCGCTGGGGTCTGAAACCCTACCTGTTTGTGCTGAATAACGATGGCTACACCATCGAAAAACTGATCCACGGTCCCAAAGCCC#AGTACAACGAAATCCAGGGCTGGGATCACCTGAGTCTGCTGCCGACCTTTGGCGCTAAAGATTACGAAACCCATCGCGTGGCTACCACGGGTGAATGGGATAAACTGACCCA#GGATAAGAGCTTTAACGATAACAGCAAGATCCGCATGATCGAAATCATGCTGCCCGTGTTTGATGCTCCCCAGAACCTGGTGGAACAGGCCAAACTGACCGCTGCCACCAAC#GCCAAACAGTAA | |
*Description of function | *Description of function | ||
| Line 18: | Line 18: | ||
'''ADH''' | '''ADH''' | ||
*Primary nucleaic acid sequence | *Primary nucleaic acid sequence | ||
| - | + | #ATGTCGATCCCCGAAACCCAGAAAGGCGTGATCTTTTACGAAAGCCACGGCAAACTGGAATACAAAGATATCCCCGTGCCCAAACCCAAAGCCAATGAACTGCTGATCAACG#TGAAATACAGTGGCGTGTGCCACACCGATCTGCACGCCTGGCATGGCGATTGGCCTCTGCCCGTGAAACTGCCCCTGGTGGGTGGCCATGAAGGCGCTGGCGTGGTGGTGGG#CATGGGCGAAAACGTGAAAGGCTGGAAAATCGGCGATTACGCTGGCATCAAATGGCTGAACGGCAGCTGCATGGCCTGCGAATACTGCGAACTGGGCAACGAAAGCAACTGT#CCCCACGCCGATCTGAGCGGCTACACCCACGATGGCAGCTTTCAGCAGTACGCCACCGCTGATGCCGTGCAGGCTGCCCACATTCCCCAGGGCACCGATCTGGCTCAGGTGG#CTCCCATTCTGTGCGCTGGCATTACCGTGTACAAAGCCCTGAAAAGCGCCAACCTGATGGCTGGCCACTGGGTCGCCATTAGTGGCGCTGCTGGCGGTCTGGGCAGTCTGGC#CGTGCAGTATGCCAAAGCCATGGGCTATCGCGTGCTGGGCATCGATGGTGGCGAAGGCAAAGAAGAACTGTTTCGCAGCATCGGTGGCGAAGTGTTTATCGATTTCACCAAA#GAAAAAGATATCGTGGGTGCCGTCCTGAAAGCCACCGATGGCGGTGCTCACGGCGTGATCAACGTCAGCGTGAGCGAAGCCGCTATCGAAGCCAGCACCCGCTACGTTCGCG#CTAATGGCACCACCGTGCTGGTCGGCATGCCTGCTGGCGCTAAATGCTGCTCGGATGTGTTTAACCAGGTGGTGAAAAGCATCAGCATCGTGGGCAGCTATGTGGGCAATCG#CGCTGATACTCGCGAAGCCCTGGATTTTTTTGCTCGCGGTCTGGTGAAAAGCCCCATCAAAGTGGTGGGCCTGAGCACCCTGCCCGAAATCTACGAAAAAATGGAAAAAGGC#CAGATCGTCGGTCGCTACGTGGTGGATACCAGCAAATAATAGGGATCC | |
*Description of function | *Description of function | ||
ADH is short for Alcohol dehydrogenase which can catalyse the generation of ethanol. | ADH is short for Alcohol dehydrogenase which can catalyse the generation of ethanol. | ||
| + | #[[Image:aaaaa.jpg]] | ||
*Acknowedgment of sources and references | *Acknowedgment of sources and references | ||
Bennetzen JL and Hall BD(1982)The primary structure of the Saccharomyces cerevisae gene for alcohol dehydrogenase.J Biol Chem.257(6):3018-25 | Bennetzen JL and Hall BD(1982)The primary structure of the Saccharomyces cerevisae gene for alcohol dehydrogenase.J Biol Chem.257(6):3018-25 | ||
| Line 27: | Line 28: | ||
'''groESL''' | '''groESL''' | ||
*Primary nucleaic acid sequence | *Primary nucleaic acid sequence | ||
| - | + | #CAGTTATCAGTTATCAGTTGTCAGGTTATTTCCACTGTTCACTGTTCACTGATAACTGTTAACTGTCCACTGTTCGGAAACCCGTACACACCAAAACATTGCCATGCTAGGA#TGGTTTTCGGTAAATTAGCACTCAGGAGTGGAGAGTGCTAACTCTGGAGAAATTAAAT | |
*Description of function | *Description of function | ||
heat-shock-responsive operon | heat-shock-responsive operon | ||
| Line 36: | Line 37: | ||
'''Rbc''' | '''Rbc''' | ||
*Primary nucleaic acid sequence | *Primary nucleaic acid sequence | ||
| - | + | #CAGTCAATGGAGAGCATTGCCATAAGTAAAGGCATCCCCTGCGTGATAAGATTACCTTCAGAAAACAGATAGTTGCTGGGTTATCGCAGATTTTTCTCGCAACCAAATAACT#GTAAATAATAACTGTCTCTGGGGCGACGGTAGGCTTTATATTGCCAAATTTCGCCCGTGGGAGAAAGCTAGGCTATTCAATGTTTATGGAGGACTGACCTAG | |
*Description of function | *Description of function | ||
RuBisCO promoter | RuBisCO promoter | ||
*Acknowedgment of sources and references | *Acknowedgment of sources and references | ||
Mutating the Synechocystis sp. PCC6803 RuBisCO promoter as a means of controlling levels of gene expression in cyanobacteria | Mutating the Synechocystis sp. PCC6803 RuBisCO promoter as a means of controlling levels of gene expression in cyanobacteria | ||
Revision as of 02:07, 20 October 2009
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Parts
PDC
- Primary nucleaic acid sequence
- ATGAGCGAAATCACCCTGGGCAAATACCTGTTTGAACGCCTGAAACAGGTGAACGTGAACACCGTGTTTGGCCTGCCTGGCGATTTTAATCTGAGCCTGCTGGATAAAATCT#ACGAAGTGGAAGGCATGCGCTGGGCTGGCAACGCCAACGAACTGAACGCTGCCTACGCTGCCGATGGCTACGCTCGCATCAAAGGCATGAGCTGTATCATCACGACCTTTGG#CGTGGGCGAACTGAGCGCTCTGAACGGCATTGCTGGCAGCTACGCCGAACACGTGGGCGTGCTGCATGTGGTGGGCGTGCCCAGCATTAGCGCTCAGGCCAAACAGCTGCTG#CTGCACCATACCCTGGGCAACGGCGATTTTACCGTGTTTCACCGCATGAGCGCCAACATCAGCGAAACCACCGCCATGATCACCGATATCGCCACCGCTCCCGCTGAAATCG#ATCGCTGCATCCGCACCACCTACGTGACCCAACGCCCTGTGTATCTGGGCCTCCCTGCCAACCTGGTGGATCTGAACGTGCCTGCCAAACTGCTGCAGACCCCCATTGATAT#GAGCCTGAAACCCAACGATGCCGAAAGCGAAAAAGAAGTGATCGATACCATCCTGGCCCTGGTGAAAGATGCCAAAAACCCCGTGATCCTGGCCGATGCCTGCTGCAGTCGC#CACGATGTGAAAGCCGAAACCAAAAAACTGATCGATCTGACCCAGTTTCCCGCTTTTGTGACCCCCATGGGCAAAGGCAGCATCGATGAACAGCACCCTCGCTACGGTGGCG#TGTATGTGGGCACCCTGAGCAAACCCGAAGTGAAAGAAGCCGTGGAAAGCGCTGATCTGATCCTGAGCGTGGGTGCCCTGCTGTCGGATTTTAACACCGGCAGCTTTAGCTA#CAGCTACAAGACCAAAAACATCGTGGAATTTCACAGCGATCACATGAAAATCCGCAACGCCACCTTTCCTGGCGTGCAGATGAAATTTGTGCTGCAGAAACTGCTGACCACC#ATTGCCGATGCCGCTAAAGGCTACAAACCCGTGGCTGTTCCCGCTCGCACCCCTGCTAATGCTGCTGTGCCTGCCAGCACCCCTCTGAAACAAGAATGGATGTGGAACCAGC#TGGGCAACTTTCTGCAAGAGGGTGATGTGGTGATCGCCGAAACCGGCACCAGCGCCTTTGGCATCAACCAGACCACCTTTCCCAACAACACCTACGGCATCAGCCAGGTGCT#GTGGGGCAGCATCGGCTTTACGACCGGTGCTACCCTGGGTGCTGCGTTTGCCGCTGAAGAAATCGATCCCAAAAAACGCGTGATTCTGTTTATCGGTGATGGCAGCCTGCAG#CTGACCGTGCAAGAAATCAGCACCATGATCCGCTGGGGTCTGAAACCCTACCTGTTTGTGCTGAATAACGATGGCTACACCATCGAAAAACTGATCCACGGTCCCAAAGCCC#AGTACAACGAAATCCAGGGCTGGGATCACCTGAGTCTGCTGCCGACCTTTGGCGCTAAAGATTACGAAACCCATCGCGTGGCTACCACGGGTGAATGGGATAAACTGACCCA#GGATAAGAGCTTTAACGATAACAGCAAGATCCGCATGATCGAAATCATGCTGCCCGTGTTTGATGCTCCCCAGAACCTGGTGGAACAGGCCAAACTGACCGCTGCCACCAAC#GCCAAACAGTAA
- Description of function
PDC is short for pyruvate decarboxylase, it is a homotetrameric enzyme Pyruvate decarboxylase which can converting pyruvate into acetaldehyde and carbon dioxide.
- Acknowedgment of sources and references
Kellermann,E., Seeboth,P.G. and Hollenberg,C.P. Analysis of the primary structure and promoter function of a pyruvate decarboxylase gene (PDC1) from Saccharomyces cerevisiae, Nucleic Acids Res. 14 (22), 8963-8977 (1986)
ADH
- Primary nucleaic acid sequence
- ATGTCGATCCCCGAAACCCAGAAAGGCGTGATCTTTTACGAAAGCCACGGCAAACTGGAATACAAAGATATCCCCGTGCCCAAACCCAAAGCCAATGAACTGCTGATCAACG#TGAAATACAGTGGCGTGTGCCACACCGATCTGCACGCCTGGCATGGCGATTGGCCTCTGCCCGTGAAACTGCCCCTGGTGGGTGGCCATGAAGGCGCTGGCGTGGTGGTGGG#CATGGGCGAAAACGTGAAAGGCTGGAAAATCGGCGATTACGCTGGCATCAAATGGCTGAACGGCAGCTGCATGGCCTGCGAATACTGCGAACTGGGCAACGAAAGCAACTGT#CCCCACGCCGATCTGAGCGGCTACACCCACGATGGCAGCTTTCAGCAGTACGCCACCGCTGATGCCGTGCAGGCTGCCCACATTCCCCAGGGCACCGATCTGGCTCAGGTGG#CTCCCATTCTGTGCGCTGGCATTACCGTGTACAAAGCCCTGAAAAGCGCCAACCTGATGGCTGGCCACTGGGTCGCCATTAGTGGCGCTGCTGGCGGTCTGGGCAGTCTGGC#CGTGCAGTATGCCAAAGCCATGGGCTATCGCGTGCTGGGCATCGATGGTGGCGAAGGCAAAGAAGAACTGTTTCGCAGCATCGGTGGCGAAGTGTTTATCGATTTCACCAAA#GAAAAAGATATCGTGGGTGCCGTCCTGAAAGCCACCGATGGCGGTGCTCACGGCGTGATCAACGTCAGCGTGAGCGAAGCCGCTATCGAAGCCAGCACCCGCTACGTTCGCG#CTAATGGCACCACCGTGCTGGTCGGCATGCCTGCTGGCGCTAAATGCTGCTCGGATGTGTTTAACCAGGTGGTGAAAAGCATCAGCATCGTGGGCAGCTATGTGGGCAATCG#CGCTGATACTCGCGAAGCCCTGGATTTTTTTGCTCGCGGTCTGGTGAAAAGCCCCATCAAAGTGGTGGGCCTGAGCACCCTGCCCGAAATCTACGAAAAAATGGAAAAAGGC#CAGATCGTCGGTCGCTACGTGGTGGATACCAGCAAATAATAGGGATCC
- Description of function
ADH is short for Alcohol dehydrogenase which can catalyse the generation of ethanol.
- Acknowedgment of sources and references
Bennetzen JL and Hall BD(1982)The primary structure of the Saccharomyces cerevisae gene for alcohol dehydrogenase.J Biol Chem.257(6):3018-25
groESL
- Primary nucleaic acid sequence
- CAGTTATCAGTTATCAGTTGTCAGGTTATTTCCACTGTTCACTGTTCACTGATAACTGTTAACTGTCCACTGTTCGGAAACCCGTACACACCAAAACATTGCCATGCTAGGA#TGGTTTTCGGTAAATTAGCACTCAGGAGTGGAGAGTGCTAACTCTGGAGAAATTAAAT
- Description of function
heat-shock-responsive operon
- Acknowedgment of sources and references
Cloning and characterization of the major groESL operon from a nitrogen-¢xing cyanobacterium Anabaena sp. strain L-31
Rbc
- Primary nucleaic acid sequence
- CAGTCAATGGAGAGCATTGCCATAAGTAAAGGCATCCCCTGCGTGATAAGATTACCTTCAGAAAACAGATAGTTGCTGGGTTATCGCAGATTTTTCTCGCAACCAAATAACT#GTAAATAATAACTGTCTCTGGGGCGACGGTAGGCTTTATATTGCCAAATTTCGCCCGTGGGAGAAAGCTAGGCTATTCAATGTTTATGGAGGACTGACCTAG
- Description of function
RuBisCO promoter
- Acknowedgment of sources and references
Mutating the Synechocystis sp. PCC6803 RuBisCO promoter as a means of controlling levels of gene expression in cyanobacteria
