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Revision as of 04:02, 20 October 2009

University of Alberta - BioBytes

Glycerol Stock

What you will need:

Overnight with bacterial growth
Screw cap tubes
Glycerol

Preparation:

Pipet 150 uL 50% glycerol into 3* properly labeled 1.5 ml screw cap tubes.
Add 350 uL of overnight culture to each tube.
Pipet up and down to gently mix.
Flash freeze in liquid N2 or dry ice/methanol bath
Place in -80 C freezer when frozen.

Anchor

If we make extra we have them if we need to send them out or want to store in several locations.

Team:Alberta/Project/ByteAssembly