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Team:Alberta/Project/ISceIdigestion
From 2009.igem.org
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| - | <h1>Linearizing | + | <h1>Linearizing BioByte Constructs by I-SceI Digestion</h1> |
<h2>What you will need:</h2> | <h2>What you will need:</h2> | ||
<ul> | <ul> | ||
<li>I-SceI | <li>I-SceI | ||
| - | <li>Biobyte Construct | + | <li>Circular Biobyte Construct |
</ul> | </ul> | ||
Latest revision as of 05:33, 20 October 2009
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Linearizing BioByte Constructs by I-SceI DigestionWhat you will need:
Digestion:For a 20 μL reaction:
Incubate at 37ºC for 1 hour |
