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Team:HKU-HKBU/Assembly
From 2009.igem.org
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(Assembly of BactoMotor) |
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{{Team:HKU-HKBU/header}} | {{Team:HKU-HKBU/header}} | ||
| - | =Assembly of BactoMotor= | + | ='''Assembly of BactoMotor'''= |
| - | == | + | =='''Introduction'''== |
Our BactoMotor is comprised of three parts – the Micro-Motor; the Directional Controller and the Speed Controller. | Our BactoMotor is comprised of three parts – the Micro-Motor; the Directional Controller and the Speed Controller. | ||
| - | == | + | =='''Prodecures'''== |
| + | ==='''Step 1. Installation of Micro-Motor'''=== | ||
| + | The Micro-Motor will be installed into a microchip container (Fig.1a, 1b) with separated chambers. Only one side of the arms of the motor is coated with Biotin (for the Directional Controller Module).[[Image:HKU-HKBU ass f1a s.PNG|frame|center|600px|'''Fig.1a:''' Photos of the microchips for micro-motor installation; A: A photo showing the chambers and the valve systems; B: Valve 3 closed; C: Valves 4, 5, 6 and 7 are closed while water is injected into the channel; L: Valve 1 and 7 closed, enclosing the crystal violet solution in the lower 4 chambers; P: The photo of the chamber system]][[Image:HKU-HKBU ass f1b.PNG|frame|center|'''Fig.1b:''' A diagram showing the Micro-Motor Module installed into a chamber inside the microchip.]] | ||
| - | + | ==='''Step 2. Introduction of Bacteria'''=== | |
| - | + | The Bacteria with both Directional Controller and Speed Controller Modules implemented will be introduced to the Micro-Motor (Fig.2). [[Image:HKU-HKBU ass f2.PNG|frame|center|'''Fig.2:''' Introduction of Bacteria into the Micro-Motor]] | |
| - | + | ||
| - | + | ==='''Step 3. Action of Directional Controller'''=== | |
| - | + | Under the effect of the Directional Controller, the Bacteria will bind onto the Micro-Motor directionally. (Fig.3a, 3b)[[Image:HKU-HKBU ass f3a.PNG|frame|center|'''Fig.3a:''' The bacteria bounded to the Micro-Motor through action of the Directional Controller Module (Plan View)]][[Image:HKU-HKBU ass f3b.PNG|frame|center|'''Fig.3b:''' A zoomed view of the Directional Controller Module at work.]] | |
| + | |||
| + | ==='''Step 4. Removing Excessive Bacteria'''=== | ||
| + | The extra bacterial cells will be removed by medium flush. (Fig. 4)[[Image:HKU-HKBU ass f3s.PNG|frame|center|'''Fig.4:''' Bacteria not binding to the Micro-Motor will be flushed away by medium flush.]] | ||
| + | |||
| + | ==='''Step 5. BactoMotor in Action'''=== | ||
| + | As the Bacteria swims, they will push the motor into rotation. (clockwise as demonstrated in Fig. 5a, 5b)[[Image:HKU-HKBU ass f4a.PNG|frame|center|'''Fig.5a:''' The Directional Rotation of the Micro-Motor achieved by the Directional Controller Module]][[Image:HKU-HKBU ass f4b.gif|frame|center|'''Fig.5b:''' An animation showing the BactoMotor in action.]] | ||
| + | |||
| + | ==='''Step 6. Using the Speed Controller'''=== | ||
| + | To utilize the Speed Controller Module, an inducer is added to the Microchip containing the Micro-Motor. Increase in concentration of the inducer will lead to an overall increase in rotational speed of the Bacto-Motor. (Fig. 6a, 6b)[[Image:HKU-HKBU ass f5a.gif|frame|center|'''Fig.6a:''' On additional of 0.5 effective dose of inducer]][[Image:HKU-HKBU ass f5b.gif|frame|center|'''Fig.6b:''' On additional of 1.0 effective dose of inducer]] | ||
{{Team:HKU-HKBU/footer}} | {{Team:HKU-HKBU/footer}} | ||
Revision as of 06:06, 20 October 2009
Contents |
Assembly of BactoMotor
Introduction
Our BactoMotor is comprised of three parts – the Micro-Motor; the Directional Controller and the Speed Controller.
Prodecures
Step 1. Installation of Micro-Motor
The Micro-Motor will be installed into a microchip container (Fig.1a, 1b) with separated chambers. Only one side of the arms of the motor is coated with Biotin (for the Directional Controller Module).
Fig.1a: Photos of the microchips for micro-motor installation; A: A photo showing the chambers and the valve systems; B: Valve 3 closed; C: Valves 4, 5, 6 and 7 are closed while water is injected into the channel; L: Valve 1 and 7 closed, enclosing the crystal violet solution in the lower 4 chambers; P: The photo of the chamber system
Fig.1b: A diagram showing the Micro-Motor Module installed into a chamber inside the microchip.
Step 2. Introduction of Bacteria
The Bacteria with both Directional Controller and Speed Controller Modules implemented will be introduced to the Micro-Motor (Fig.2).
Fig.2: Introduction of Bacteria into the Micro-Motor
Step 3. Action of Directional Controller
Under the effect of the Directional Controller, the Bacteria will bind onto the Micro-Motor directionally. (Fig.3a, 3b)
Fig.3a: The bacteria bounded to the Micro-Motor through action of the Directional Controller Module (Plan View)
Fig.3b: A zoomed view of the Directional Controller Module at work.
Step 4. Removing Excessive Bacteria
The extra bacterial cells will be removed by medium flush. (Fig. 4)
Fig.4: Bacteria not binding to the Micro-Motor will be flushed away by medium flush.
Step 5. BactoMotor in Action
As the Bacteria swims, they will push the motor into rotation. (clockwise as demonstrated in Fig. 5a, 5b)
Fig.5a: The Directional Rotation of the Micro-Motor achieved by the Directional Controller Module
Fig.5b: An animation showing the BactoMotor in action.
Step 6. Using the Speed Controller
To utilize the Speed Controller Module, an inducer is added to the Microchip containing the Micro-Motor. Increase in concentration of the inducer will lead to an overall increase in rotational speed of the Bacto-Motor. (Fig. 6a, 6b)
Fig.6a: On additional of 0.5 effective dose of inducer
Fig.6b: On additional of 1.0 effective dose of inducer
Sponsors
