Team:HKUST/Protocols/gel cut

From 2009.igem.org

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<li><a href="https://2009.igem.org/Team:HKUST/OdorantSensoring">Odorant Sensoring</a></li>
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<li><a href="https://2009.igem.org/Team:HKUST/OdorantSensing">Odorant Sensing</a></li>
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<li><a href="https://2009.igem.org/Team:HKUST/AttranctantProduction">Attranctant Production</a></li>
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<li><a href="https://2009.igem.org/Team:HKUST/AttractantProduction">Attractant Production</a></li>
<li><a href="https://2009.igem.org/Team:HKUST/ToxinProduction">Toxin Production</a></li>
<li><a href="https://2009.igem.org/Team:HKUST/ToxinProduction">Toxin Production</a></li>

Latest revision as of 09:55, 20 October 2009

Salt and Soap template

a

Cutting bands on the gel

Purpose: To extract specific DNA fragment.

Procedure:

a.Visualize the bands under UV light. Use long-wavelength UV to minimize damage to the DNA.
b.Cut the band with a clean razor blade.
c.Turn the gel slice on its side to trim off extra agarose. Place the gel in a microcentrifuge tube.

Tips:

EB staining is needed for higher resolution under UV light.

Safety tips:

1. ermission is needed from technician before doing this! Follow the safety instruction in the room.

2.When using UV light, protect your skin by wearing safety goggles or a face shield, gloves, and a lab coat.

HKUST

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