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September/21 September 2009
From 2009.igem.org
(Difference between revisions)
(New page: 1.colony check<br> sample no. no. of colony 27 ++ 28 ++ 30 ++ 35 + 45 + 46 1 48 ...) |
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ligation O/N | ligation O/N | ||
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Latest revision as of 11:11, 20 October 2009
1.colony check
sample no. no. of colony 27 ++ 28 ++ 30 ++ 35 + 45 + 46 1 48 ++ 50 +++ 51 + 1-1F +++ 46 1 2-9A + 2-3I ++ 3-7G ++ 3-7C ++ 1-8K +++
2.transformation
sample no 36 , 37 , 38 , 39 , 40
3.min prep
sample no. conc. 27 285 ng/uL 28 181 30 194 35 173 45 290 48 35 50 153 51 146 1-1F 132
4.digation
X1-A
| Vector | Insert | ||
|---|---|---|---|
| 51 | 4 | 1-12M | 8 |
| SpeI | 1 | XbaI | 1 |
| PstI | 1 | PstI | 1 |
| No.2 | 2 | No.2 | 2 |
| dH2O | 12 | dH2O | 8 |
| total | 20uL | total | 20uL |
X2-A
| Vector | Insert | ||
|---|---|---|---|
| 50 | 4 | 1-12M | 8 |
| SpeI | 1 | XbaI | 1 |
| PstI | 1 | PstI | 1 |
| No.2 | 2 | No.2 | 2 |
| dH2O | 12 | dH2O | 8 |
| total | 20uL | total | 20uL |
X3-A
| Vector | Insert | ||
|---|---|---|---|
| 31 | 16 | 1-12M | 4 |
| SpeI | 1 | XbaI | 1 |
| PstI | 1 | PstI | 1 |
| No.2 | 2 | No.2 | 2 |
| dH2O | 0 | dH2O | 12 |
| total | 20uL | total | 20uL |
X4-A
| Vector | Insert | ||
|---|---|---|---|
| 32 | 16 | 1-12M | 4 |
| SpeI | 1 | XbaI | 1 |
| PstI | 1 | PstI | 1 |
| No.2 | 2 | No.2 | 2 |
| dH2O | 0 | dH2O | 12 |
| total | 20uL | total | 20uL |
↓
37°C, 2hr
↓
gel electrophoresis
gel cut
purification
↓
ligation O/N
