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Team:Illinois/Protocols
From 2009.igem.org
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*Agarose Gel | *Agarose Gel | ||
**50mL 0.5x TBE buffer | **50mL 0.5x TBE buffer | ||
| - | **Agarose (To determine number of grams to use, multiply volume by percentage of gel. For example, use 1.5g of agarose in a 3% agarose gel.) | + | **Agarose (To determine number of grams to use, multiply volume (50mL) by percentage of gel. For example, use 1.5g of agarose in a 3% agarose gel.) |
**2.5μL ethidium bromide | **2.5μL ethidium bromide | ||
Questions about our Wiki page? Please email Dave Korenchan at [mailto:korench1@illinois.edu korench1@illinois.edu]. | Questions about our Wiki page? Please email Dave Korenchan at [mailto:korench1@illinois.edu korench1@illinois.edu]. | ||
Revision as of 17:46, 26 June 2009
Protocols
This page describes protocols or includes links to protocols used in our project. Recipes used are also listed in a separate section. Protocols are organized by category.
Standard
- [http://www.biochem.northwestern.edu/morimoto/research/Protocols/I.%20Prokaryotes/A.%20Bacteriology.pdf Bacterial Culture Basics (media/plate preparation, growth, streaking, and storage)]
- [http://www.methodbook.net/dna/agarogel.html Agarose Gel Electrophoresis Protocol]
- [http://www.5prime.com/media/29661/perfectprep%20spin%20mini%20kit%20manual_5prime_1048299_072007.pdf Miniprep Protocol (PerfectPrep Spin Mini Kit)]
Recipes
- LB Growth Media
- 1L dH20
- 10g NaCl
- 5g yeast extract
- 10g Bacto-tryptone
- Agarose Gel
- 50mL 0.5x TBE buffer
- Agarose (To determine number of grams to use, multiply volume (50mL) by percentage of gel. For example, use 1.5g of agarose in a 3% agarose gel.)
- 2.5μL ethidium bromide
Questions about our Wiki page? Please email Dave Korenchan at korench1@illinois.edu.
