Revision as of 13:19, 20 October 2009

Home of iGEMOSAKA wiki

NOTES

Calendar

July
M	T	W	T	F	S	S
		[http://2009.igem.org/wiki/index.php?title=July/1_July_2009&action=edit 1]	[http://2009.igem.org/wiki/index.php?title=July/2_July_2009&action=edit 2]	[http://2009.igem.org/wiki/index.php?title=July/3_July_2009&action=edit 3]	[http://2009.igem.org/wiki/index.php?title=July/4_July_2009&action=edit 4]	[http://2009.igem.org/wiki/index.php?title=July/5_July_2009&action=edit 5]
[http://2009.igem.org/wiki/index.php?title=July/6_July_2009&action=edit 6]	[http://2009.igem.org/wiki/index.php?title=July/7_July_2009&action=edit 7]	[http://2009.igem.org/wiki/index.php?title=July/8_July_2009&action=edit 8]	[http://2009.igem.org/wiki/index.php?title=July/9_July_2009&action=edit 9]	[http://2009.igem.org/wiki/index.php?title=July/10_July_2009&action=edit 10]	[http://2009.igem.org/wiki/index.php?title=July/11_July_2009&action=edit 11]	[http://2009.igem.org/wiki/index.php?title=July/12_July_2009&action=edit 12]
[http://2009.igem.org/wiki/index.php?title=July/13_July_2009&action=edit 13]	[http://2009.igem.org/wiki/index.php?title=July/14_July_2009&action=edit 14]	[http://2009.igem.org/wiki/index.php?title=July/15_July_2009&action=edit 15]	[http://2009.igem.org/wiki/index.php?title=July/16_July_2009&action=edit 16]	[http://2009.igem.org/wiki/index.php?title=July/17_July_2009&action=edit 17]	[http://2009.igem.org/wiki/index.php?title=July/18_July_2009&action=edit 18]	[http://2009.igem.org/wiki/index.php?title=July/19_July_2009&action=edit 19]
[http://2009.igem.org/wiki/index.php?title=July/20_July_2009&action=edit 20]	[http://2009.igem.org/wiki/index.php?title=July/21_July_2009&action=edit 21]	[http://2009.igem.org/wiki/index.php?title=July/22_July_2009&action=edit 22]	[http://2009.igem.org/wiki/index.php?title=July/23_July_2009&action=edit 23]	[http://2009.igem.org/wiki/index.php?title=July/24_July_2009&action=edit 24]	[http://2009.igem.org/wiki/index.php?title=July/25_July_2009&action=edit 25]	[http://2009.igem.org/wiki/index.php?title=July/26_July_2009&action=edit 26]
[http://2009.igem.org/wiki/index.php?title=July/27_July_2009&action=edit 27]	[http://2009.igem.org/wiki/index.php?title=July/28_July_2009&action=edit 28]	[http://2009.igem.org/wiki/index.php?title=July/29_July_2009&action=edit 29]	[http://2009.igem.org/wiki/index.php?title=July/30_July_2009&action=edit 30]	[http://2009.igem.org/wiki/index.php?title=July/31_July_2009&action=edit 31]

August
M	T	W	T	F	S	S
					[http://2009.igem.org/August/1_August_2009 1]	[http://2009.igem.org/August/2_August_2009 2]
[http://2009.igem.org/August/3_August_2009 3]	[http://2009.igem.org/August/4_August_2009 4]	[http://2009.igem.org/August/5_August_2009 5]	[http://2009.igem.org/August/6_August_2009 6]	[http://2009.igem.org/August/7_August_2009 7]	[http://2009.igem.org/August/8_August_2009 8]	[http://2009.igem.org/August/9_August_2009 9]
[http://2009.igem.org/August/10_August_2009 10]	[http://2009.igem.org/August/11_August_2009 11]	[http://2009.igem.org/August/12_August_2009 12]	[http://2009.igem.org/August/13_August_2009 13]	[http://2009.igem.org/August/14_August_2009 14]	[http://2009.igem.org/August/15_August_2009 15]	[http://2009.igem.org/August/16_August_2009 16]
[http://2009.igem.org/August/17_August_2009 17]	[http://2009.igem.org/August/18_August_2009 18]	[http://2009.igem.org/August/19_August_2009 19]	[http://2009.igem.org/August/20_August_2009 20]	[http://2009.igem.org/August/21_August_2009 21]	[http://2009.igem.org/August/22_August_2009 22]	[http://2009.igem.org/August/23_August_2009 23]
[http://2009.igem.org/August/24_August_2009 24]	[http://2009.igem.org/August/25_August_2009 25]	[http://2009.igem.org/August/26_August_2009 26]	[http://2009.igem.org/August/27_August_2009 27]	[http://2009.igem.org/August/28_August_2009 28]	[http://2009.igem.org/August/29_August_2009 29]	[http://2009.igem.org/August/30_August_2009 30]
[http://2009.igem.org/August/31_August_2009 31]

September
M	T	W	T	F	S	S
	[http://2009.igem.org/September/1_September_2009 1]	[http://2009.igem.org/September/2_September_2009 2]	[http://2009.igem.org/September/3_September_2009 3]	[http://2009.igem.org/September/4_September_2009 4]	[http://2009.igem.org/September/5_September_2009 5]	[http://2009.igem.org/September/6_September_2009 6]
[http://2009.igem.org/September/7_September_2009 7]	[http://2009.igem.org/September/8_September_2009 8]	[http://2009.igem.org/September/9_September_2009 9]	[http://2009.igem.org/September/10_September_2009 10]	[http://2009.igem.org/September/11_September_2009 11]	[http://2009.igem.org/September/12_September_2009 12]	[http://2009.igem.org/September/13_September_2009 13]
[http://2009.igem.org/September/14_September_2009 14]	[http://2009.igem.org/September/15_September_2009 15]	[http://2009.igem.org/September/16_September_2009 16]	[http://2009.igem.org/September/17_September_2009 17]	[http://2009.igem.org/September/18_September_2009 18]	[http://2009.igem.org/September/19_September_2009 19]	[http://2009.igem.org/September/20_September_2009 20]
[http://2009.igem.org/September/21_September_2009 21]	[http://2009.igem.org/September/22_September_2009 22]	[http://2009.igem.org/September/23_September_2009 23]	[http://2009.igem.org/September/24_September_2009 24]	[http://2009.igem.org/September/25_September_2009 25]	[http://2009.igem.org/September/26_September_2009 26]	[http://2009.igem.org/September/27_September_2009 27]
[http://2009.igem.org/September/28_September_2009 28]	[http://2009.igem.org/September/29_September_2009 29]	[http://2009.igem.org/September/30_September_2009 30]

October
M	T	W	T	F	S	S
			[http://2009.igem.org/August/1_October_2009 1]	[http://2009.igem.org/August/2_October_2009 2]	[http://2009.igem.org/August/3_October_2009 3]	[http://2009.igem.org/August/4_October_2009 4]
[http://2009.igem.org/August/5_October_2009 5]	[http://2009.igem.org/August/6_October_2009 6]	[http://2009.igem.org/August/7_October_2009 7]	[http://2009.igem.org/August/8_October_2009 8]	[http://2009.igem.org/August/9_October_2009 9]	[http://2009.igem.org/August/10_October_2009 10]	[http://2009.igem.org/August/11_October_2009 11]
[http://2009.igem.org/August/12_October_2009 12]	[http://2009.igem.org/August/13_October_2009 13]	[http://2009.igem.org/August/14_October_2009 14]	[http://2009.igem.org/August/15_October_2009 15]	[http://2009.igem.org/August/16_October_2009 16]	[http://2009.igem.org/August/17_October_2009 17]	[http://2009.igem.org/August/18_October_2009 18]
[http://2009.igem.org/wiki/index.php?title=August/19_October_2009&action=edit 19]	[http://2009.igem.org/August/20_October_2009 20]	[http://2009.igem.org/wiki/index.php?title=August/21_October_2009&action=edit 21]	[http://2009.igem.org/wiki/index.php?title=August/22_October_2009&action=edit 22]	[http://2009.igem.org/wiki/index.php?title=August/23_October_2009&action=edit 23]	[http://2009.igem.org/wiki/index.php?title=August/24_October_2009&action=edit 24]	[http://2009.igem.org/wiki/index.php?title=August/25_October_2009&action=edit 25]
[http://2009.igem.org/wiki/index.php?title=August/26_October_2009&action=edit 26]	[http://2009.igem.org/wiki/index.php?title=August/27_October_2009&action=edit 27]	[http://2009.igem.org/wiki/index.php?title=August/28_October_2009&action=edit 28]	[http://2009.igem.org/wiki/index.php?title=August/29_October_2009&action=edit 29]	[http://2009.igem.org/wiki/index.php?title=August/30_October_2009&action=edit 30]	[http://2009.igem.org/wiki/index.php?title=August/31_October_2009&action=edit 31]

November
M	T	W	T	F	S	S
						[http://2009.igem.org/wiki/index.php?title=November/1_November_2009&action=edit 1]
[http://2009.igem.org/wiki/index.php?title=November/2_November_2009&action=edit 2]	[http://2009.igem.org/wiki/index.php?title=November/3_November_2009&action=edit 3]	[http://2009.igem.org/wiki/index.php?title=November/4_November_2009&action=edit 4]	[http://2009.igem.org/wiki/index.php?title=November/5_November_2009&action=edit 5]	[http://2009.igem.org/wiki/index.php?title=November/6_November_2009&action=edit 6]	[http://2009.igem.org/wiki/index.php?title=November/7_November_2009&action=edit 7]	[http://2009.igem.org/wiki/index.php?title=November/8_November_2009&action=edit 8]
[http://2009.igem.org/wiki/index.php?title=November/9_November_2009&action=edit 9]	[http://2009.igem.org/wiki/index.php?title=November/10_November_2009&action=edit 10]	[http://2009.igem.org/wiki/index.php?title=November/11_November_2009&action=edit 11]	[http://2009.igem.org/wiki/index.php?title=November/12_November_2009&action=edit 12]	[http://2009.igem.org/wiki/index.php?title=November/13_November_2009&action=edit 13]	[http://2009.igem.org/wiki/index.php?title=November/14_November_2009&action=edit 14]	[http://2009.igem.org/wiki/index.php?title=November/15_November_2009&action=edit 15]
[http://2009.igem.org/wiki/index.php?title=November/16_November_2009&action=edit 16]	[http://2009.igem.org/wiki/index.php?title=November/17_November_2009&action=edit 17]	[http://2009.igem.org/wiki/index.php?title=November/18_November_2009&action=edit 18]	[http://2009.igem.org/wiki/index.php?title=November/19_November_2009&action=edit 19]	[http://2009.igem.org/wiki/index.php?title=November/20_November_2009&action=edit 20]	[http://2009.igem.org/wiki/index.php?title=November/21_November_2009&action=edit 21]	[http://2009.igem.org/wiki/index.php?title=November/22_November_2009&action=edit 22]
[http://2009.igem.org/wiki/index.php?title=November/23_November_2009&action=edit 23]	[http://2009.igem.org/wiki/index.php?title=November/24_November_2009&action=edit 24]	[http://2009.igem.org/wiki/index.php?title=November/25_November_2009&action=edit 25]	[http://2009.igem.org/wiki/index.php?title=November/26_November_2009&action=edit 26]	[http://2009.igem.org/wiki/index.php?title=November/27_November_2009&action=edit 27]	[http://2009.igem.org/wiki/index.php?title=November/28_November_2009&action=edit 28]	[http://2009.igem.org/wiki/index.php?title=November/29_November_2009&action=edit 29]
[http://2009.igem.org/wiki/index.php?title=November/30_November_2009&action=edit 30]

Registry Parts

COLOR
K204033 mOrange

placI
K204041 placI+GFP
K204042 placI+YFP
K204043 placI+CFP
K204044 placI+RFP
K204048 placI+mOrange

pcI
K204058 pcI+YFP
K204059 pcI+CFP
K204062 pcI+mOrange

ptetR
K204052 ptetR+mOrange

Carotenoid
K204064 crtE+crtB
K204065 crtI+crtY
K204068 crtZ

K204076 placI+crtE+crtB
K204077 ptetR+crtE+crtB
K204091 placI+crtE+crtB+crtI+crtY
K204095 placI+crtE+crtB+crtZ
K204096 ptetR+crtE+crtB+crtZ

gradation
K204069 cI repressor
K204086 3O₆HSL receiver+RFP+cI repressor

SIGNAL (Quorum Sensing)

AI-1
K204047 LasI generater

C₄HSL
K204040 RhlI (generater)
K204031 RhlR (receiver)
K204702 RhlR+GFP

3OH,C₁₄ : 1-HSL
K204025 cinI (generater)
K204032 cinR (receiver)

3O₆HSL
K204051 LuxR (receiver)
K204700 LuxR+GFP

MOTILITY
K204018 ptetR+EspE

K204501 RBS+FliH
K204502 ptetR+FliH
K204503 pT7+FliH
K204504 C₄HSL receiver +FliH

Lab Note

WET Lab

Week 1 : 8/2~8/8
Brainstorming , parts planning , prepared competent cell
transformation and miniprep from MIT plate

week 2 : 8/9~8/15
start assembly parts (K204001 ~ K204006) , transformation and mini prep from MIT

Week 3 : 8/16~8/22
assembly parts (~K204015) , parts planning

Week 4 : 8/23~8/29
assembly parts (~K204021)

Week 5 : 8/30~9/5
assembly parts (~K204030)

Week 6 : 9/6~9/12
assembly parts (~K204046)

Week 7 : 9/13~9/19
assembly parts (~K204050)

Week 8 : 9/20~9/26
assembly parts (~K204056)
TEST : sensitivity of inducer and receptor
Sample : 3O₆HSL , C₄HSL , AI-1 , 3OH,C₁₄:1-HSL
Result : Not confirm the fluorescence...

Week 9 : 9/27~10/3
assembly parts (~K204066 , X1~X8) , color (fluorescence) intensity check

Week 10 : 10/4~10/10
assembly parts (~K204078)
Lux receiver sensitivity test , color (fluorescence) intensity check
sequence check : generator and receiver
3O₆HSL , C₄HSL , AI-1 , 3OH,C₁₄:1-HSL
Get the parts from the Chiba University Team. , Thanks chiba team

Week 11 : 10/11~10/17
assembly parts (~K2040104)
sequence check : FliH etc…
send Osaka team parts to MIT
FINISH!
Osaka team assembled about 120 parts!

etc

Under construction

@@ Line 127: / Line 127: @@
    <div id="CollapsiblePanel3" class="CollapsiblePanel">
    <div class="CollapsiblePanelTab" tabindex="0">
-     <h2>Implement</h2></div>
+     <h2>Lab Note</h2></div>
    <div class="CollapsiblePanelContent">
-     <p>Under construction</p><br>
+     <p>WET Lab</p><br>
+Week 1 : 8/2~8/8<br>
+Brainstorming , parts planning , prepared competent cell<br>
+transformation and miniprep from MIT plate<br>
+<br>
+week 2 : 8/9~8/15<br>
+start assembly parts (K204001 ~ K204006) , transformation and mini prep from MIT
+<br>
+<br>
+Week 3 : 8/16~8/22<br>
+assembly parts (~K204015)  ,  parts planning<br>
+<br>
+Week 4 : 8/23~8/29<br>
+assembly parts (~K204021)<br>
+<br>
+Week 5 : 8/30~9/5<br>
+assembly parts (~K204030)<br>
+<br>
+Week 6 : 9/6~9/12<br>
+assembly parts (~K204046)<br>
+<br>
+Week 7 : 9/13~9/19<br>
+assembly parts (~K204050)<br>
+<br>
+Week 8 : 9/20~9/26<br>
+assembly parts (~K204056)<br>
+TEST : sensitivity of inducer and receptor <br>
+Sample : 3O<sub>6</sub>HSL , C<sub>4</sub>HSL , AI-1 , 3OH,C<sub>14</sub>:1-HSL<br>
+Result : Not confirm the fluorescence...<br>
+<br>
+Week 9 : 9/27~10/3<br>
+assembly parts (~K204066 , X1~X8) , color (fluorescence) intensity check<br>
+<br>
+Week 10 : 10/4~10/10<br>
+assembly parts (~K204078)<br>
+Lux receiver sensitivity test  ,  color (fluorescence) intensity check<br>
+sequence check : generator and receiver<br>
+O<sub>6</sub>HSL , C<sub>4</sub>HSL , AI-1 , 3OH,C<sub>14</sub>:1-HSL<br>
+Get the parts from the Chiba University Team. , Thanks chiba team
+<br>
+<br>
+Week 11 : 10/11~10/17<br>
+assembly parts (~K2040104)<br>
+sequence check : FliH etc…<br>
+send Osaka team parts to MIT<br>
+FINISH!<br>
+<b>Osaka team assembled about 120 parts!</b><br>
    </div>
    </div>
@@ Line 135: / Line 199: @@
 <div id="CollapsiblePanel4" class="CollapsiblePanel">
    <div class="CollapsiblePanelTab" tabindex="0">
-     <h2>uno</h2></div>
+     <h2>etc</h2></div>
    <div class="CollapsiblePanelContent">
      <p>Under construction</p><br>

Team:Osaka/NOTES

From 2009.igem.org

Revision as of 13:19, 20 October 2009

NOTES

Calendar

Registry Parts

Lab Note

etc