Team:Illinois/MicC

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Revision as of 19:01, 26 June 2009

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MicC Target-GFP Fusion

Primers Used:

June 15

PCRs of sRNA genes and target sequences were performed using E. coli extracted chromosomal DNA and primers. We were able to purify the ompC gene on a 3% agarose gel, however no band was visible from the micC PCR product.

June 16

Another PCR of the micC gene was done. Results should be analyzed tomorrow.

June 17

We performed a restriction enzyme digest of our small RNA and its target sequence. We then ran a gel to purify the DNA.

@@ Line 3: / Line 3: @@
 == '''MicC Target-GFP Fusion''' ==
-'''People in Group:''' Phil Bell, Sonam Tanna, Davel Patel
-'''Purpose:''' We want to fuse the MicC target sequence from the OmpC gene to the GFP gene on a low-copy plasmid and transform this into E. coli cells along with a high-copy plasmid carrying the MicC gene.  We expect to see translational repression of GFP by the small RNA MicC.
-'''Protocol(s) Used:''' (links to protocols page)
-'''Recipe(s) Used:'''
 '''Primers Used:'''