Team:Wash U/Calendar-Home/26 June 2009

From 2009.igem.org

(Difference between revisions)
Line 28: Line 28:
Recipe for loading:
Recipe for loading:
-
1 &muL loading dye + 8 &muL sample per lane
+
1 μL loading dye + 8 μL sample per lane
105 volts
105 volts

Revision as of 19:29, 26 June 2009

-Received Sigma shipment of tubes, reagents, and restriction enzymes

-Made TAE buffer for electrophoresis gels

-Ran PCR-amplified biobrick parts (from yesterday) on 0.8% agarose gel, to check size

-Gel layout:

Lane 1 (from left): 100 bp ladder

2: Sample A

3: Sample B

4: Sample C

5: Sample D

6: Sample E

7: Sample F

8: Sample G

9: 0.5 kb ladder


Recipe for loading:

1 μL loading dye + 8 μL sample per lane

105 volts