Team:Wash U/Calendar-Home/26 June 2009

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Revision as of 19:37, 26 June 2009



Friday June 26, 2009

-Received Sigma shipment of tubes, reagents, and restriction enzymes

-Made TAE buffer for electrophoresis gels

-Ran PCR-amplified biobrick parts (from yesterday) on 0.8% agarose gel, to check size

-Gel layout:

Lane 1 (from left): 100 bp ladder

2: Sample A

3: Sample B

4: Sample C

5: Sample D

6: Sample E

7: Sample F

8: Sample G

9: 0.5 kb ladder


Recipe for loading:

1 μL loading dye + 8 μL sample per lane

105 volts


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