METU-Gene

From 2009.igem.org

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<br>Wound Healing velocity will increase as described in the video. Effects of Thymosin beta 4 are similar with synthesis of Epidermal Growth Factor and Keratinocyte Growth Factor our system synthesizing.  
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<br>Wound healing, or wound repair, is an intricate process in which the skin (or some other organ) repairs itself after injury. In normal skin, the epidermis (outermost layer) and dermis (inner or deeper layer) exists in a steady-state equilibrium, forming a protective barrier against the external environment. Once the protective barrier is broken, the normal (physiologic) process of wound healing is immediately set in motion. The classic model of wound healing is divided into three or four sequential, yet overlapping, phases: (1) hemostasis (not considered a phase by some authors), (2) inflammatory, (3) proliferative and (4) remodeling.
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Although we plan to use Thymosin beta 4 next year, Our Wound Healing Mechanism will be upgraded with this future implication.
 
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Thymosin beta 4 which consists of 68 a.a accelerated skin wound healing in a rat model of a full thickness wound where the epithelial layer was destroyed. When Tb4 was applied topically to the wound or injected into the animal, epithelial layer restoration in the wound was increased 42% by day four and 61% by day seven, after treatment, compared to untreated. Furthermore, Tb4 stimulated collagen deposition in the wound and angiogenesis. Tb4 accelerated keratinocyte migration, resulting in the wound contracting by more than 11%, compared to untreated wounds, to close the skin gap in the wound. An analysis of skin sections (histological observations) showed that the Tb4 treated wounds healed faster than the untreated. Proof of accelerated cell migration was also seen in vitro, where Tb4 increased keratinocyte migration two to threefold, within four to five hours after treatment, compared to untreated keratinocytes.
 
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Revision as of 15:00, 20 October 2009


METU



w5

note
Calendar and daily notes of lab stuff.
more

w6

100
Parts and device that we contribute.
more

w7

100
Protocols and hard information about project


w5

note
Planing of Human Application and info. about usage and function
100

w6

100
All projects, we have thought and improved through iGEM Competition Time
more

w7

100
Acknowledgements and references being the guide for our project.

w6

more
150

w7

Wound healing, or wound repair, is an intricate process in which the skin (or some other organ) repairs itself after injury. In normal skin, the epidermis (outermost layer) and dermis (inner or deeper layer) exists in a steady-state equilibrium, forming a protective barrier against the external environment. Once the protective barrier is broken, the normal (physiologic) process of wound healing is immediately set in motion. The classic model of wound healing is divided into three or four sequential, yet overlapping, phases: (1) hemostasis (not considered a phase by some authors), (2) inflammatory, (3) proliferative and (4) remodeling.
w7
w6