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Team:Nevada/Team
From 2009.igem.org
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Revision as of 17:10, 20 October 2009
Back: Dr. Shintani, J, Alex, Tyler. Middle: Janice, Leigh, Joey, Chris. Front: Nick and Dr. Howard
Contents |
Who we are
Advisors
- Advisor 1: Dr. Christie Howard
- Advisor 2: Dr. Patricia Ellison
- Advisor 3: Dr. Dave Shintani
- Grad Student 1: Chad Sanada
Undergrads
- Student 1: Nick Tschernia
- Student 2: Tony Moschetti
- Student 3: Joey Khwaja
- Student 4: Chris Martinez
- Student 5: Janice Cho
- Student 6: Leigh Armijo
- Student 7: Sheena Jones
- Student 8: Tyler Peterson
- Student 9: J Malikowski
- Student 10: Alex Dussaq
What we did
- Entered the Nevada Team into the iGEM Competition a week before the submission date.
- Organized weekly meetings for project brainstorming and timeline.
- Recruited the iGEM Team through a highly selective process (whoever was left at the end of the brainstorming sessions).
- Organized the source plate distribution and transformation of the basic parts.
- Sequence analysis of the Biobrick parts formed.
- Primer design for adding Biobrick restriction sites for formation of Biobrick parts.
- Formulated the original idea of using cinnamaldehyde as a mosquito larvicide.
- Performed initial research on the use of cinnamaldehyde.
- Helped perform a three-way ligation with part BBa_R0011 (lacI promoter), part BBa_B0034 (ribsome-binding site), and part pSB3C5 (chloromphenicol resistant backbone) to create part BBa_K262000.
- Helped conduct a three-way ligation to create final construct with the parts: BBa_K262000 and BBa_K262002.
- Helped perform a three-way ligation with part BBa_R0011 (lacI promoter), part BBa_B0034 (ribsome-binding site), and part pSB3C5 (chloromphenicol resistant backbone) to create part BBa_K262000.
- Helped conduct a three-way ligation to create a construct with BBa_K262000, 4-coumarate:CoA ligase, and BBa_B0014.
- In charge of maintaining and propagating Wolffia and Lemna plant species.
- Generated callus culture in preparation for gene transformation.
- Topo-cloned cinnamoyl-CoA reductase (gene 3)for preparation of a Biobrick part.
- Made Biobrick part containing cinnamoyl-CoA reductase (gene 3) and double terminator (BBa_K262002)
- Transformed 4-coumarate-CoA ligase (gene 2) and cinnamoyl-CoA reductase (gene 3 - BBa_K262001) into Biobrick backbones.
- Induced expression in non-biobrick expression vector containing 4-coumarate-CoA ligase (gene 2). Detection of product formation at 311 nm. Further studies will be conducted in the near future.
- Helped to troubleshoot minipreps and other protocols. Resident expert on minipreps.
- Trained Tyler to be an excellent Molecular Biologist.
- Advisory role at lab meetings (How does the Harper lab do it?)
- Major player in media preparation, agarose gels, stock solutions
- Helped with minipreps and E. coli transformation.
- Made db3.1 and NEB10 competent cells.
- Overall goal was to upregulate the cinnamaldehyde pathway in plants Wolffia and various Lemna species
- Transformation of cinnamoyl-CoA Reductase (Gene 3) into TOPO D Vector
- Used LR CLonase Protocal to move cinnamoyl-CoA Reductase (Gene 3) into pK7WG2D.1 Binary Vector which will be used to grow up Agrobacterium, which will be used to infect the plants Wolffia and Lemna species
- Made db3.1 and NEB10 competent cells.
- Researched into making a Biobrick plant part.
- Designed the iGEM Nevada Team shirts.
Meet the Team
