Team:IPN-UNAM-Mexico/Notebook

From 2009.igem.org

(Difference between revisions)
Line 34: Line 34:
|B0015
|B0015
|E, X
|E, X
 +
|}
 +
 +
 +
We propose this restrictions for standar assembly for the digest we use a 17 hours incubation at 37º camera.
 +
Digest Mix:
 +
 +
 +
{| border="0.5"
 +
! ERCO RI - SPEI
 +
! Per reaction
 +
|-
 +
|Plasmidic DNA
 +
|align="right" | 3μl
 +
|-
 +
|Enzima ECORI
 +
|align="right" | 2μl
 +
|-
 +
|Enzima SPEI
 +
|align="right" | 2μl
 +
|-
 +
|Buffer NBE
 +
|align="right" | 2μl
 +
|-
 +
|BSA
 +
|align="right" | 0.5μl
 +
|-
 +
|H2O
 +
|align="right" | 10.5μl
 +
|-
 +
!Total
 +
|align="right" | 20μl
 +
|}
 +
 +
 +
{| border="0.5"
 +
! ECORI - XBAI
 +
!Per reaction
 +
|-
 +
|Plasmidic DNA
 +
|align="right" | 3μl
 +
|-
 +
|Enzima ECORI
 +
|align="right" | 2μl
 +
|-
 +
|Enzima XBAI
 +
|align="right" | 2μl
 +
|-
 +
|Buffer NBE
 +
|align="right" | 2μl
 +
|-
 +
|BSA
 +
|align="right" | 0.5μl
 +
|-
 +
|H20
 +
|align="right" | 10.5μl
 +
|-
 +
!Total
 +
|align="right" | 20μl
|}
|}

Revision as of 17:16, 20 October 2009


BannerUNAM.jpg

As summer proyect our experimental work began at 07 - july - 2009 using 2008 bioparts catalog, unfurtunly it dosen’t work properly, we had to much troubles to take out DNA and transform, E. Coli for this we had to delay and request 2009 catalog.

Our proyect uses 29 bioparts, 12 ligations and diferent strategys to make it functional as follow:

Month-icon.png June

Month-icon.png July

07-July-2009

We digest the follow biobriks with ECORI (E), SPEI (S), and XBAI (X).

Biobrick Enzime restriction
R0079 E, S
F1610 E, X
B0034 E, S
C0078 E, X
C0079 E, X
B0015 E, X


We propose this restrictions for standar assembly for the digest we use a 17 hours incubation at 37º camera. Digest Mix:


ERCO RI - SPEI Per reaction
Plasmidic DNA 3μl
Enzima ECORI 2μl
Enzima SPEI 2μl
Buffer NBE 2μl
BSA 0.5μl
H2O 10.5μl
Total 20μl


ECORI - XBAI Per reaction
Plasmidic DNA 3μl
Enzima ECORI 2μl
Enzima XBAI 2μl
Buffer NBE 2μl
BSA 0.5μl
H20 10.5μl
Total 20μl


Month-icon.png August

Month-icon.png September

Month-icon.png October


Banner footer UNAM2.jpg

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