Team:HKU-HKBU/Motor Preliminary Trials

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Revision as of 18:31, 20 October 2009

Preliminary test using a Membrane

We used Immobilon-P transfer membrane to evaluate the performance of streptavidin-biotin binding of bacteria onto a surface. Before cutting down the membrane into small pieces, pre-activation should be applied to it first [Link 1]. After the pre-activation, the membrane was first sheared into strings manually by using scissors, with the width and thickness both around 100μm. Then, we used Leica-crytomicrotome to cut the “threads” into small fragments, with the length of 60μm. Thus, we got the motors, whose demention was aproximately 100μm×60μm×100μm. Since the surface of membrane had already been activated, the polar-expression bacteria could bind onto such biotin-coated motors.

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 {{Team:HKU-HKBU/header}}
-==Membrane Version==
+==Preliminary test using a Membrane==
-We used Immobilon-P transfer membrane as the material for motor. Before cutting down the membrane into small pieces, pre-activation should be applied to it first [Link 1]. After the pre-activation, the membrane was first sheared into strings manually by using scissors, with the width and thickness both around 100μm. Then, we used Leica-crytomicrotome to cut the “threads” into small fragments, with the length of 60μm. Thus, we got the motors, whose demention was aproximately 100μm×60μm×100μm. Since the surface of membrane had already been activated, the polar-expression bacteria could bind onto such biotin-coated motors.
+We used Immobilon-P transfer membrane to evaluate the performance of streptavidin-biotin binding of bacteria onto a surface. Before cutting down the membrane into small pieces, pre-activation should be applied to it first [Link 1]. After the pre-activation, the membrane was first sheared into strings manually by using scissors, with the width and thickness both around 100μm. Then, we used Leica-crytomicrotome to cut the “threads” into small fragments, with the length of 60μm. Thus, we got the motors, whose demention was aproximately 100μm×60μm×100μm. Since the surface of membrane had already been activated, the polar-expression bacteria could bind onto such biotin-coated motors.
 {{Team:HKU-HKBU/footer}}