Team:Kyoto/CiC/Notebook/week2

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(New page: ==Week 1 : Construction of HIV TAT LALLA peptide and 11R LALLA peptide == {{:Team:Kyoto/Pad_start_dates}} ===Monday, September 10=== ====To Do on 24 July==== *PCR (HIV TAT LALAA peptide,11...)
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Revision as of 18:45, 20 October 2009

Contents

Week 1 : Construction of HIV TAT LALLA peptide and 11R LALLA peptide

Monday, September 10

To Do on 24 July

  • PCR (HIV TAT LALAA peptide,11R LALLA peptide)
  • Electrophoresis
  • Restriction Enzyme Digestion

Results

Electrophoresis

Kyoto e 090724 1.png

  • Very clear bands were found. (See the gel image in right!)
  • The bands are listed below.
Parts and Plasmid length
3-O-23-A-4
Parts length / bp2837487
Plasmid length / bp32663266
Digestion
  • Digested with EcoR1 / EcoR1 and Pst1 and run on a gel.
Sample list
NO.Sample NameEnzyme
13-O-2Eco R1, PST1
23-A-4Eco R1, PST1
33-O-2Eco R1
43-A-4Eco R1



Tuseday, September 11

To Do on 24 July

  • Ligation(HIV TAT LALAA peptide,11R LALLA peptide)
  • Transformation

Results

Ligation
  • Digested with EcoR1 / EcoR1 and Pst1 and run on a gel.
Sample list
NO.Sample NameEnzyme
13-O-2Eco R1, PST1
23-A-4Eco R1, PST1
33-O-2Eco R1
43-A-4Eco R1
Electrophoresis

Kyoto e 090724 1.png

  • Very clear bands were found. (See the gel image in right!)
  • The bands are listed below.
Parts and Plasmid length
3-O-23-A-4
Parts length / bp2837487
Plasmid length / bp32663266

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