Team:Freiburg bioware/cloning1

From 2009.igem.org

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<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;pJS419_Phagemid-dummy <span class="art-PostHeader"></span> </h3>
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<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
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<img src="https://static.igem.org/mediawiki/2009/thumb/b/b1/Freiburg09_PJS419_dummy.jpg/600px-Freiburg09_PJS419_dummy.jpg" name="pJS419 Vector with weak SD sequence" width="480" height="480" />
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<br><p align="center">pJS419 Vector with weak SD sequence</p>
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<li> <a href="http://partsregistry.org/Part:BBa_K243034">link to part registry</a>
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<h4>Commented GenBank file</h4>
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<li> <a href="https://static.igem.org/mediawiki/2009/d/d9/Freiburg09_PJS419_dummy.txt">pJS419.txt</a></li>
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Revision as of 18:57, 20 October 2009

FREiGEM

 Index



 Parts

 FokA_pMA

back to index

FokA in pMA Vector

  • link to part registry
    		• nucleotid sequence of ORF-1:
    ATGGCCGGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAA ACTGAAATATGTGCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTA ATAGTACCCAAGACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATG AAAGTCTATGGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCTGA TGGTGCCATTTATACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTTG ATACCAAAGCCTATAGCGGGGGTTATAACCTGCCAATTGGTCAGGCTGAT GAGATGCAGCGTTATGTGAAAGAGAACCAGACTCGTAACAAACACATCAA CCCGAACGAATGGTGGAAAGTGTATCCGTCAAGCGTTACAGAGTTCAAAT TCCTGTTCGTGAGCGGCCATTTTAAAGGCAACTATAAAGCACAGCTGACC CGTCTGAACCATAAAACCAATAGCAATGGCGCCGTTCTGTCAGTAGAAGA GCTGCTGATTGGCGGTGAAATGATCAAAGCCGGGACCCTGACACTGGAAG AAGTTCGCCGTAAATTCAACAATGGGGAGATCAATTTTACCGGTTAA

    AA sequence of ORF-1:

    MAGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVMEFFMKVYGYRGKHL GGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQRYVKENQTRNKHINPNE WWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHKTNSNGAVLSVEELLIGGEMIKAGTL TLEEVRRKFNNGEINFTG

    Commented GenBank file

  • FokA_pMA.txt



    •  Foki_pMA

    back to index

    Foki in pMA Vector

  • link to part registry
    		• nucleotid sequence of ORF-1:
    ATGGCCGGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAA ACTGAAATATGTGCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTA ATAGTACCCAAGACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATG AAAGTCTATGGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCAGC AGGTGCCATTTATACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTGG CCACAAAAGCGTATTCTGGCGGTTATAATCTGCCGATTGGTCAGGCTGAT GAGATGGAACGTTATGTGGAAGAGAATCAGACCCGTAACAAACATCTGAA CCCGAACGAATGGTGGAAAGTGTATCCGTCAAGTGTCACCGAGTTCAAAT TTCTGTTCGTGAGCGGCCACTTTAAAGGCAACTATAAAGCCCAGCTGACT CGTCTGAACCATATCACCAATAGCAATGGGGCAGTGCTGAGTGTTGAGGA ACTGCTGATCGGTGGAGAAATGATCAAAGCAGGCACCCTGACTCTGGAAG AAGTTCGCCGTAAATTCAACAATGGCGAGATCAATTTTACCGGTTAA

    AA sequence of ORF-1:

    MAGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVMEFFMKVYGYRGKHL GGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIGQADEMERYVEENQTRNKHLNPNE WWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNSNGAVLSVEELLIGGEMIKAGTL TLEEVRRKFNNGEINFTG

    Commented GenBank file

  • Foki_pMA.txt



    •  Dig_pMA

    back to index

    Dig in pMA Vector

  • link to part registry
    		• nucleotid sequence of ORF-1:
    ATGGCCGGCGACGTGTACCACGACGGCGCCTGCCCCGAAGTGAAGCCCGT GGACAACTTCGACTGGTCCCAGTACCACGGCAAGTGGTGGCAGGTGGCCG CTTATCCCGACCACATCACCAAGTACGGCAAGTGCGGCTGGGCCGAGTAC ACCCCCGAGGGCAAGAGCGTGAAGGTGTCCCGGTACAGCGTGATCCACGG CAAAGAGTACTTCAGCGAGGGCACCGCCTACCCTGTGGGCGACAGCAAGA TCGGCAAGATCTACCACAGCTACACCATCGGCGGCGTGACCCAGGTGGGC GTGAGCAACGTGCTGTCCACCGACAACAAGAACTACATCATCGGCTACTT TTGCAGATACGACGAGGACAAGAAGGGCCACTGGGACGCCGTGTGGGTGC TGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAGACCGCCGTGGAGAAC TACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAAACTGGTGTACAGCGA CTTCTCCGAGGCCGCCTGCAAAGTGAACAACAGCAACTGGTCCCACCCCC AGTTCGAAAAGACCGGTTAA

    AA sequence of ORF-1:

    MAGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTPEGKSVKVS RYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNYIIGYFCRY DEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKVNNSNW SHPQFEKTG

    Commented GenBank file

  • Dig_pMA.txt



    •  FluA_pMA

    back to index

    FluA in pMA Vector

  • link to part registry
    		• nucleotid sequence of ORF-1:
    ATGGCCGGCGACGTGTACCACGACGGCGCCTGCCCCGAAGTGAAGCCCGT GGACAACTTCGACTGGTCCCAGTACCACGGCAAGTGGTGGGAGGTGGCCA AGTACCCCAGCCCCAACGGCAAGTATGGCAAGTGCGGCTGGGCCGAGTAC ACCCCCGAGGGCAAGAGCGTGAAGGTGTCCAGATACGACGTGATCCACGG CAAAGAATACTTCATGGAAGGCACCGCCTACCCCGTGGGCGACAGCAAGA TCGGCAAGATCTACCACAGCCGGACCGTGGGCGGCTACACCAGAAAGACC GTGTTCAACGTGCTGTCCACCGACAACAAGAACTACATCATCGGCTACAG CTGCCGCTACGACGAGGACAAGAAGGGCCACTGGGACCACGTGTGGGTGC TGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAGACCGCCGTGGAGAAC TACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAAACTGGTGTACAGCGA CTTCAGCGAGGCCGCCTGCAAAGTGAACAACACCGGTTAA

    AA sequence of ORF-1:

    MAGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTPEGKSVKVS RYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNYIIGYSCRY DEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKVNNTG

    Commented GenBank file

  • FluA_pMA.txt



    •  DsbA_pMA

    back to index

    DsbA in pMA Vector

  • link to part registry
    		• nucleotid sequence of ORF-1:
    ATGAAAAAGATTTGGCTGGCGCTGGCTGGTTTAGTTTTAGCGTTTAGCGC ATCGGCGACCGGTTA

    AA sequence of ORF-1:

    MKKIWLALAGLVLAFSASATG

    Commented GenBank file

  • DsbA_pMA.txt



    •  StrepTag_pMA (iGEM team Freiburg 08)

    back to index

    StrepTag in pMA Vector

  • link to part registry
    		• nucleotid sequence of ORF-1:
    ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGTT

    AA sequence of ORF-1:

    MAGWSHPQFEKTG

    Commented GenBank file

  • StrepTag_pMA.txt



    •  HisTag_pMA (iGEM team Freiburg 08)

    back to index

    HisTag in pMA Vector

  • link to part registry
    		• nucleotid sequence of ORF-1:
    GCCGGCCATCATCATCATCATCATACCGGT

    AA sequence of ORF-1:

    AGHHHHHHTG

    Commented GenBank file

  • HisTag_pMA.txt



    •  shortlinker26_pMA

    back to index

    shortlinker26 in pMA Vector

  • link to part registry
    		• nucleotid sequence of ORF-1:
    ATGGCCGGCGGTGGTTCTGGTACCGGT

    AA sequence of ORF-1:

    MAGGGSGTG

    Commented GenBank file

  • shortlinker26_pMA.txt



    •  Middellinker38_pMA

    back to index

    Middellinker38 in pMA Vector

  • link to part registry
    		• nucleotid sequence of ORF-1:
    ATGGCCGGCGGTGGTTCTGGTGGTGGTTCTGGTACCGGT

    AA sequence of ORF-1:

    MAGGGSGGGSGTG

    Commented GenBank file

  • Middellinker38_pMA.txt



    •  LongLinkerA50_pMA

    back to index

    LongLinkerA50 in pMA Vector

  • link to part registry
    		• nucleotid sequence of ORF-1:
    ATGGCCGGCGGTGGTTCTGGTGGTGGTTCTGGTACCGGT

    AA sequence of ORF-1:

    MAGGGSGGGSGTG

    Commented GenBank file

  • LongLinkerA50_pMA.txt



    •  GSAT_linker_pMA

    back to index

    GSAT_linker in pMA Vector

  • link to part registry
    		• nucleotid sequence of ORF-1:
    GAGCTCGAATTCGCGGCCGCTTCTAGATGGCCGGCGGTGGTTCTGCCGGT GGCTCCGGTTCTGGCTCCAGCGGTGGCAGCTCTGGTGCGTCCGGCACGGG TACTGCGGGTGGCACTGGCAGCGGTTCCGGTACTGGCTCTGGCACCGGTA ATACTAGTAGCGGCCGCTGCAGGGTACC

    AA sequence of ORF-1:

    ELEFAAASRWPAVVLPVAPVLAPAVAALVRPARVLRVALAAVPVLALAPVILVAAAAGY

    Commented GenBank file

  • GSAT_linker_pMA.txt



    •  SEG_Linker_pMA

    back to index

    SEG_Linker in pMA Vector

  • link to part registry
    		• nucleotid sequence of ORF-1:
    GAGCTCGAATTCGCGGCCGCTTCTAGATGGCCGGCGGTGGTTCTGGCGGC GGTTCTGAAGGTGGCGGCTCCGAAGGCGGCGGCAGCGAGGGCGGTGGTAG CGAAGGTGGTGGCTCCGAGGGTGGCGGTTCCGGCGGCGGTAGCACCGGTA ATACTAGTAGCGGCCGCTGCAGGGTACC

    AA sequence of ORF-1:

    ELEFAAASRWPAVVLAAVLKVAAPKAAAARAVVAKVVAPRVAVPAAVAPVILVAAAAGY

    Commented GenBank file

  • SEG_Linker_pMA.txt



    •  Split_Linker_pMA (iGEM team Freiburg 08)

    back to index

    Split_Linker in pMA Vector

  • link to part registry
    		• nucleotid sequence of ORF-1:
    ATGGCCGGCCGACCAGCCTGTAAGATTCCAAATGACCTGAAGCAGAAAGT TATGAATCACACCGGT

    AA sequence of ORF-1:

    MAGRPACKIPNDLKQKVMNHTG

    Commented GenBank file

  • Split_Linker_pMA.txt



    •  YFP(Venus)_pGA14 (iGEM team Freiburg 08)

    back to index

    YFP(Venus) in pGA14 Vector

  • link to part registry
    		• nucleotid sequence of ORF-1:
    ATGGCCGGCATGAGCAAAGGCGAAGAACTGTTTACCGGCGTGGTGCCGAT TCTGGTGGAACTGGATGGTGATGTGAACGGCCATAAATTTAGCGTGAGCG GCGAAGGCGAAGGTGATGCGACCTATGGCAAACTGACCCTGAAACTGATT TGCACCACCGGCAAACTGCCGGTTCCGTGGCCGACCCTGGTTACCACCCT GGGCTATGGCCTGCAATGCTTTGCGCGTTATCCGGATCATATGAAACAGC ACGATTTCTTTAAAAGCGCCATGCCGGAAGGCTATGTGCAGGAACGCACC ATCTTTTTTAAAGATGATGGCAACTATAAAACCCGTGCGGAAGTGAAATT TGAAGGCGATACCCTGGTGAACCGTATTGAACTGAAAGGCATCGATTTCA AAGAAGATGGCAACATTCTGGGCCATAAACTGGAATATAACTACAACAGC CATAACGTGTATATCACCGCGGATAAACAGAAAAACGGCATCAAAGCGAA CTTTAAAATCCGCCACAACATTGAAGATGGCGGCGTGCAGCTGGCCGATC ATTATCAGCAGAACACCCCGATTGGTGATGGCCCGGTGCTGCTGCCGGAT AACCATTATCTGAGCTACCAGAGCAAACTGAGCAAAGATCCGAACGAAAA ACGTGACCATATGGTGCTGCTGGAATTTGTGACCGCGGCCGGTATTACCC ATGGCATGGATGAACTGTATAAAACCGGTTAA

    AA sequence of ORF-1:

    MAGMSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKLICTTGKLPVPW PTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGD TLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKANFKIRHNIEDGGVQ LADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLEFVTAAGITHGMDELY KTG

    Commented GenBank file

  • YFP(Venus)_pGA14.txt



    •  pJS418_Phagemid-dummy

    back to index

    pJS418 Vector with strong SD sequence

  • link to part registry

    • Commented GenBank file

  • pJS418.txt
    		•



     pJS419_Phagemid-dummy

    back to index

    pJS419 Vector with weak SD sequence

  • link to part registry

    • Commented GenBank file

  • pJS419.txt
    		•





    contact:  freigem09@googlemail.com

    Retrieved from "http://2009.igem.org/Team:Freiburg_bioware/cloning1"