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Team:DTU Denmark/notebookredoxilator
From 2009.igem.org
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<font size="4"><b>Activities relating to the redoxilator</b></font><br> | <font size="4"><b>Activities relating to the redoxilator</b></font><br> | ||
| + | <font size="3"><b>July 20th</b></font><br> | ||
| + | <p align="justify">Construction of biobricks: The restriction products were evaluated on a 1% agarose gel (see figure 2).</p><br> | ||
| + | </html> | ||
| + | [[image:DTUgel1.jpg]] | ||
| + | <html> | ||
| + | <p align="center"><i>Figure 2: Restriction analysis of the three biobricks.</i> | ||
| + | <font size="3"><b>July 19th</b></font><br> | ||
| + | <p align="justify">Construction of biobricks: The three plasmids were purified according to the protocol (link to protocol). Following the purification of the plasmids, a restriction analysis was performed using SpeI enzyme (10U) and incubated at 37ºC overnight.</p><br> | ||
| + | <font size="3"><b>July 18th</b></font><br> | ||
| + | <p align="justify">Construction of biobricks: Some colonies were picked from the transformation plates and made overnight cultures in liquid media.</p><br> | ||
| + | <font size="3"><b>July 17th</b></font><br> | ||
| + | <p align="justify">Construction of biobricks: </p><br> | ||
| + | <font size="3"><b>July 16th</b></font><br> | ||
| + | <p align="justify">Construction of biobricks: </p><br> | ||
| + | |||
| + | <font size="3"><b>July 15th</b></font><br> | ||
| + | <p align="justify">Construction of biobricks: </p><br> | ||
| + | |||
| + | <font size="3"><b>July 14th</b></font><br> | ||
| + | <p align="justify">Construction of biobricks: </p><br> | ||
| + | |||
| + | <font size="3"><b>July 13th</b></font><br> | ||
| + | <p align="justify">Construction of biobricks: </p><br> | ||
Revision as of 19:19, 20 October 2009
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Activities relating to our two sub-projects: - The Redoxilator - The USERTM assembly standard |
Day-to-day activities Activities relating to the redoxilator July 20th Construction of biobricks: The restriction products were evaluated on a 1% agarose gel (see figure 2). 
Figure 2: Restriction analysis of the three biobricks.
July 19th Construction of biobricks: The three plasmids were purified according to the protocol (link to protocol). Following the purification of the plasmids, a restriction analysis was performed using SpeI enzyme (10U) and incubated at 37ºC overnight. July 18th Construction of biobricks: Some colonies were picked from the transformation plates and made overnight cultures in liquid media. July 17th Construction of biobricks: July 16th Construction of biobricks: July 15th Construction of biobricks: July 14th Construction of biobricks: July 13th Construction of biobricks: |
Work process Our team works parallel in smaller sub-teams. Some of us work hard in the lab, while others are in the process of developing software and the in silico model of the Redoxilator system. However, we constantly keep each other updated, and meet often to exchange ideas and take turns at the different tasks, thus exhausting all of our combined knowledge in every aspect of this project. |
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