From 2009.igem.org
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Revision as of 20:12, 20 October 2009
The project
The redoxilator
- Genetic design
- Applications and perspectives
- Results
- Safety considerations
The USER assembly standard
- USER fusion of biobricks
USER fusion primer design software
- Abstract
- Instructions
- Output format
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The project
Results
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Achievements
Redox sensing device
Two novel genes have been designed and synthesized each comprised of 5 genetic elements. Together they function as a device termed the Redoxilator that can sense the internal redox state of a yeast cell, and output a reporter signal.
Biobricks
DNA of several new biobricks have been designed and submitted including a yeast optimized GFP reporter protein, a protein degradation sequence and a fast degradable yeast GFP. (Bronze medal)
We have demonstrated that our USER fusion biobrick works as expected and documented it (silver medal)
USER fusion Assembly standard
A new biobrick assembly standard that allows the rapid construction of multi-part devices have been developed and documented. The assembly standard offers many benefits: All restriction sites are allowed, multiple biobricks can be joined in one step, the result is scar-free making it ideal for protein fusions and more. (Gold medal)
USER-fusion primer design software
A novel and very useful software tool have been developed that can automatically design the optimal primers for USER fusion assembly of 2-9 biobricks, taking several parameters into account.
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