Team:Wash U/Calendar-Home/1 September 2009

From 2009.igem.org

(Difference between revisions)
Line 1: Line 1:
 +
{{WashUback}}
 +
9/1 Minipreps
9/1 Minipreps
Line 39: Line 41:
The products were ligated in the following ratios after gel purification for 1 hr at 22 deg C, followed by 20 min at 80 deg C:
The products were ligated in the following ratios after gel purification for 1 hr at 22 deg C, followed by 20 min at 80 deg C:
 +
 +
 +
 +
 +
<div style="text-align: right;">
 +
<div style="float: left;">
 +
[[Team:Wash_U/Calendar-Home/31 August 2009|Previous entry]]</div>
 +
[[Team:Wash_U/Calendar-Home/14 September 2009|Next entry]]</div>
 +
<div style="clear: left;"></div>
 +
 +
<center>[[Team:Wash U/Notebook|Back to Calendar]]</center>
 +
 +
 +
 +
 +
{{WashUbottom}}

Revision as of 20:45, 20 October 2009

9/1 Minipreps

number notation concentration
A RBS30_cph8 168.0
B ompR_term_ompC_GFP 161.0
C ompR_term_ompC_pucB/A 188.0

Did 2 digests of RBS30_cph8 with 1.5 ug each according to prefix-insertion instructions from the Silver lab- cut with EcoRI and PstI (A1 and A2)

Did 1 digest each of 1.5 ug of ompR_term_ompC_GFP (B) and ompR_term_ompC_pucB/A (C) as the vector for prefix insertion- cut with EcoRI and XbaI.

The Results of this digest are:

The first 4 lanes are the two rxns for RBS30_cph8. The top band is the one that was cutout (ab. 2.3 kb)

The next 2 lanes are ompR_term_ompC_GFP and the last two are ompR_term_ompC_pucB/A

9 1 gel purify.jpg


Concentrations: of products after Gel purification:

A1) A2) B) C)


The products were ligated in the following ratios after gel purification for 1 hr at 22 deg C, followed by 20 min at 80 deg C:



Previous entry
Next entry
Back to Calendar



Retrieved from "http://2009.igem.org/Team:Wash_U/Calendar-Home/1_September_2009"