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| <div class="r"></div> | | <div class="r"></div> |
| <ul class="art-menu"> | | <ul class="art-menu"> |
- | <li><a href="https://2009.igem.org/Team:Freiburg_bioware" | + | <li><a href="https://2009.igem.org/Team:Freiburg_bioware"><span |
- | class="active"><span class="l"></span><span
| + | class="l"></span><span class="r"></span><span |
- | class="r"></span><span class="t">Home</span></a></li>
| + | class="t">Home</span></a></li> |
| <li><a href="https://2009.igem.org/Team:Freiburg_bioware/Team"><span | | <li><a href="https://2009.igem.org/Team:Freiburg_bioware/Team"><span |
| class="l"></span><span class="r"></span><span | | class="l"></span><span class="r"></span><span |
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| </li> | | </li> |
| <li><a | | <li><a |
- | href="https://2009.igem.org/Team:Freiburg_bioware/Team/Project"><span | + | href="https://2009.igem.org/Team:Freiburg_bioware/Project"><span |
| class="l"></span><span class="r"></span><span | | class="l"></span><span class="r"></span><span |
| class="t">The | | class="t">The |
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| Practice</span></a> | | Practice</span></a> |
| <ul> | | <ul> |
- | <li><a href="#">D'oh! Something's missing here.</a> | + | <li><a |
- | <ul>
| + | href="https://2009.igem.org/Team:Freiburg_bioware/Human_Practice/Ethics">Ethics</a> |
- | <li><a href="#">1</a> </li>
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- | <li><a href="#">2</a> </li>
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- | <li><a href="#">3</a> </li>
| + | |
- | </ul>
| + | |
| </li> | | </li> |
- | <li><a href="#">4</a></li> | + | <li><a |
- | <li><a href="#">5</a></li>
| + | href="https://2009.igem.org/Team:Freiburg_bioware/Human_Practice/Safety">Safety</a></li> |
| </ul> | | </ul> |
| </li> | | </li> |
- | <li><a href="https://2009.igem.org/Team:Freiburg_bioware/Notebook"><span class="l"></span><span | + | <li><a |
| + | href="https://2009.igem.org/Team:Freiburg_bioware/Notebook" |
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| class="r"></span><span class="t">Notebook</span></a></li> | | class="r"></span><span class="t">Notebook</span></a></li> |
| <li><a | | <li><a |
- | href="https://2009.igem.org/Team:Freiburg_bioware/cloning"><span | + | href="https://2009.igem.org/Team:Freiburg_bioware/cloning1"><span |
| class="l"></span><span class="r"></span><span | | class="l"></span><span class="r"></span><span |
- | class="t">Parts</span></a></li> | + | class="t">Parts</span></a> |
| + | <ul> |
| + | <li><a |
| + | href="https://2009.igem.org/Team:Freiburg_bioware/cloning1">Basic |
| + | Parts</a></li> |
| + | <li><a |
| + | href="https://2009.igem.org/Team:Freiburg_bioware/cloning">Composite |
| + | Parts</a></li> |
| + | </ul> |
| + | </li> |
| <li><a | | <li><a |
| href="https://2009.igem.org/Team:Freiburg_bioware/Collaboration"><span | | href="https://2009.igem.org/Team:Freiburg_bioware/Collaboration"><span |
| class="l"></span><span class="r"></span><span | | class="l"></span><span class="r"></span><span |
| class="t">Collaboration</span></a></li> | | class="t">Collaboration</span></a></li> |
| + | <li><a |
| + | href="https://2009.igem.org/Team:Freiburg_bioware/Modeling"><span |
| + | class="l"></span><span class="r"></span><span |
| + | class="t">Modeling</span></a></li> |
| </ul> | | </ul> |
| </div> | | </div> |
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| <div class="art-Post-inner"> | | <div class="art-Post-inner"> |
| <div class="art-PostMetadataHeader"> | | <div class="art-PostMetadataHeader"> |
- | <h2 style="border-bottom: none;" class="art-PostHeaderIcon-wrapper"> <img | + | <h2 class="art-PostHeaderIcon-wrapper"> <img |
- | alt="" | + | style="width: 28px; height: 25px;" alt="" |
| src="https://static.igem.org/mediawiki/2009/2/2a/Freiburg09_Post_tanne_2.png" /> | | src="https://static.igem.org/mediawiki/2009/2/2a/Freiburg09_Post_tanne_2.png" /> |
- | July<span class="art-PostHeader"></span> </h2>
| + | July<span class="art-PostHeader"></span> </h2> |
| </div> | | </div> |
- | <div style="text-align: left;"><br />
| + | <br /> |
- | </div> | + | <br /> |
- | <div style="text-align: left;">
| + | |
| <h3>01.07.09, 8:30-13:00 Manuel, Hannes</h3> | | <h3>01.07.09, 8:30-13:00 Manuel, Hannes</h3> |
| <br /> | | <br /> |
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| <tbody> | | <tbody> |
| <tr> | | <tr> |
- | <td align="center"> | + | <td align="center"> <img |
- | <img
| + | |
| src="https://static.igem.org/mediawiki/2009/e/e7/Freiburg09_010709_Exprssion2_trim.jpg" | | src="https://static.igem.org/mediawiki/2009/e/e7/Freiburg09_010709_Exprssion2_trim.jpg" |
| name="SDS-Gel of the expression; Lanes: Marker (prestained broad range 7-200 kDa), T7+Tt 1h..2h..4h, T7+Aa 1h..2h..4h, BL21+Aa 1h..2h..4h" | | name="SDS-Gel of the expression; Lanes: Marker (prestained broad range 7-200 kDa), T7+Tt 1h..2h..4h, T7+Aa 1h..2h..4h, BL21+Aa 1h..2h..4h" |
| height="150" width="225" /><br /> | | height="150" width="225" /><br /> |
- | <p align="center">SDS-Gel of the expression;<br /> | + | <p align="center"><small>SDS-Gel of the |
| + | expression;<br /> |
| Lanes: Marker (prestained broad range 7-200 kDa),<br /> | | Lanes: Marker (prestained broad range 7-200 kDa),<br /> |
| T7+Tt 1h..2h..4h,<br /> | | T7+Tt 1h..2h..4h,<br /> |
| T7+Aa 1h..2h..4h,<br /> | | T7+Aa 1h..2h..4h,<br /> |
- | BL21+Aa 1h..2h..4h</p> | + | BL21+Aa 1h..2h..4h</small></p> |
| </td> | | </td> |
| </tr> | | </tr> |
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| template. Different program: nr. 25 IGEM.AM -> no result. next | | template. Different program: nr. 25 IGEM.AM -> no result. next |
| step should be to do a gradient PCR this afternoon. Just talk to Sven | | step should be to do a gradient PCR this afternoon. Just talk to Sven |
- | to get the details. | + | to get the details. </li> |
- | </li>
| + | |
| <li>inoculated T7+Tt, T7+Aa and BL21+Aa in 3 ml LB-Kan for a | | <li>inoculated T7+Tt, T7+Aa and BL21+Aa in 3 ml LB-Kan for a |
- | new round of expression at 27°C. | + | new round of expression at 27°C. </li> |
- | </li>
| + | |
| <li>idea from Sven: do a westernblot on the isolated proteins | | <li>idea from Sven: do a westernblot on the isolated proteins |
| from the AGO-Expression to find out whether the AGO-protein is present | | from the AGO-Expression to find out whether the AGO-protein is present |
- | or not. | + | or not. </li> |
- | </li>
| + | |
| </ul> | | </ul> |
| <h3>01.07.09, 14:00-18:00 Isabel, Laura</h3> | | <h3>01.07.09, 14:00-18:00 Isabel, Laura</h3> |
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| <li>gradient PCR from 60°-70°C with 8 tubes | | <li>gradient PCR from 60°-70°C with 8 tubes |
| each of vector 258 diluted and undiluted, we put it in the cold room | | each of vector 258 diluted and undiluted, we put it in the cold room |
- | -> gel has to be done tomorrow | + | -> gel has to be done tomorrow </li> |
- | </li>
| + | <li>we made 100ml 1M Tris-HCl pH=6,8 </li> |
- | <li>we made 100ml 1M Tris-HCl pH=6,8 | + | |
- | </li>
| + | |
| <li>we inoculated 4ml LB with cells from the CAT-vector #258 | | <li>we inoculated 4ml LB with cells from the CAT-vector #258 |
- | glycerol stock and put it into the 37°C room | + | glycerol stock and put it into the 37°C room </li> |
- | </li>
| + | |
| <li>to do: | | <li>to do: |
| -plasmid preparation of the inoculated cells including the CAT vector</li> | | -plasmid preparation of the inoculated cells including the CAT vector</li> |
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| <li>-fetch new dNTPs of the NEB freezer (snd floor)</li> | | <li>-fetch new dNTPs of the NEB freezer (snd floor)</li> |
| <li>-alterations of PCR-program: only one step but 30 times, | | <li>-alterations of PCR-program: only one step but 30 times, |
- | with temperature gradient 55-65°C (Sven can help) | + | with temperature gradient 55-65°C (Sven can help) </li> |
- | </li>
| + | |
| </ul> | | </ul> |
| <h3>02.07.09, 09:00-18:00 Hannes, 14:00-18:30 Laura</h3> | | <h3>02.07.09, 09:00-18:00 Hannes, 14:00-18:30 Laura</h3> |
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| <ul> | | <ul> |
| <li>AGO: expression of Aa and Tt (T7+Tt, T7+Aa, BL21+Aa) at | | <li>AGO: expression of Aa and Tt (T7+Tt, T7+Aa, BL21+Aa) at |
- | 27°C. samples after 0, 1.5 and 4 hours | + | 27°C. samples after 0, 1.5 and 4 hours </li> |
- | </li>
| + | <li>plasmid preparation of inoculated labvector 258 </li> |
- | <li>plasmid preparation of inoculated labvector 258 | + | <li>agarose gel with gradient pcr -> didn't work </li> |
- | </li>
| + | |
- | <li>agarose gel with gradient pcr -> didn't work | + | |
- | </li>
| + | |
| <li>nanodrop with primer and labvector -> concentrations | | <li>nanodrop with primer and labvector -> concentrations |
- | were good | + | were good </li> |
- | </li>
| + | |
| <li>search for a 5 cutter restriction enzyme for the test | | <li>search for a 5 cutter restriction enzyme for the test |
- | digest for tomorrow: FOKI! | + | digest for tomorrow: FOKI! </li> |
- | </li>
| + | |
| <li><b>NanoDrop data</b></li> | | <li><b>NanoDrop data</b></li> |
| </ul> | | </ul> |
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| <td class="xl6520978" | | <td class="xl6520978" |
| style="height: 15.75pt; width: 70pt;" height="21" | | style="height: 15.75pt; width: 70pt;" height="21" |
- | width="93">Sample | + | width="93"><small>Sample |
- | ID</td> | + | ID</small></td> |
| <td class="xl6320978" style="width: 48pt;" | | <td class="xl6320978" style="width: 48pt;" |
- | width="64">User ID</td> | + | width="64"><small>User ID</small></td> |
| <td class="xl6320978" style="width: 65pt;" | | <td class="xl6320978" style="width: 65pt;" |
- | width="86">Date<span style=""> </span></td> | + | width="86"><small>Date<span style=""> </span></small></td> |
| <td class="xl6320978" style="width: 48pt;" | | <td class="xl6320978" style="width: 48pt;" |
- | width="64">Time<span style=""> </span></td> | + | width="64"><small>Time<span style=""> </span></small></td> |
| <td class="xl6320978" style="width: 48pt;" | | <td class="xl6320978" style="width: 48pt;" |
- | width="64">ng/ul<span style=""> </span></td> | + | width="64"><small>ng/ul<span style=""> </span></small></td> |
| <td class="xl6320978" style="width: 48pt;" | | <td class="xl6320978" style="width: 48pt;" |
- | width="64">A260<span style=""> </span></td> | + | width="64"><small>A260<span style=""> </span></small></td> |
| <td class="xl6320978" style="width: 48pt;" | | <td class="xl6320978" style="width: 48pt;" |
- | width="64">A280<span style=""> </span></td> | + | width="64"><small>A280<span style=""> </span></small></td> |
| <td class="xl6320978" style="width: 48pt;" | | <td class="xl6320978" style="width: 48pt;" |
- | width="64">260/280<span style=""> </span></td> | + | width="64"><small>260/280<span style=""> </span></small></td> |
| <td class="xl6420978" style="width: 48pt;" | | <td class="xl6420978" style="width: 48pt;" |
- | width="64">260/230<span style=""> </span></td> | + | width="64"><small>260/230<span style=""> </span></small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl6620978" style="height: 15pt;" | | <td class="xl6620978" style="height: 15pt;" |
- | height="20">Prfwd</td> | + | height="20"><small>Prfwd</small></td> |
- | <td class="xl1520978">FreiGEM</td> | + | <td class="xl1520978"><small>FreiGEM</small></td> |
- | <td class="xl6820978" align="right">02.07.2009</td> | + | <td class="xl6820978" align="right"><small>02.07.2009</small></td> |
- | <td class="xl6920978" align="right">16:05</td> | + | <td class="xl6920978" align="right"><small>16:05</small></td> |
- | <td class="xl1520978" align="right">2511.96</td> | + | <td class="xl1520978" align="right"><small>2511.96</small></td> |
- | <td class="xl1520978" align="right">50.239</td> | + | <td class="xl1520978" align="right"><small>50.239</small></td> |
- | <td class="xl1520978" align="right">25.571</td> | + | <td class="xl1520978" align="right"><small>25.571</small></td> |
- | <td class="xl1520978" align="right">1.96</td> | + | <td class="xl1520978" align="right"><small>1.96</small></td> |
- | <td class="xl7020978" align="right">2.27</td> | + | <td class="xl7020978" align="right"><small>2.27</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl6620978" style="height: 15pt;" | | <td class="xl6620978" style="height: 15pt;" |
- | height="20">Prrev</td> | + | height="20"><small>Prrev</small></td> |
- | <td class="xl1520978">FreiGEM</td> | + | <td class="xl1520978"><small>FreiGEM</small></td> |
- | <td class="xl6820978" align="right">02.07.2009</td> | + | <td class="xl6820978" align="right"><small>02.07.2009</small></td> |
- | <td class="xl6920978" align="right">16:07</td> | + | <td class="xl6920978" align="right"><small>16:07</small></td> |
- | <td class="xl1520978" align="right">1779.18</td> | + | <td class="xl1520978" align="right"><small>1779.18</small></td> |
- | <td class="xl1520978" align="right">35.584</td> | + | <td class="xl1520978" align="right"><small>35.584</small></td> |
- | <td class="xl1520978" align="right">22.292</td> | + | <td class="xl1520978" align="right"><small>22.292</small></td> |
- | <td class="xl1520978" align="right">1.6</td> | + | <td class="xl1520978" align="right"><small>1.6</small></td> |
- | <td class="xl7020978" align="right">1.89</td> | + | <td class="xl7020978" align="right"><small>1.89</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15.75pt;" height="21"> | | <tr style="height: 15.75pt;" height="21"> |
| <td class="xl6720978" style="height: 15.75pt;" | | <td class="xl6720978" style="height: 15.75pt;" |
- | height="21">#258Plasmid</td> | + | height="21"><small>#258Plasmid</small></td> |
- | <td class="xl7120978">FreiGEM</td> | + | <td class="xl7120978"><small>FreiGEM</small></td> |
- | <td class="xl7220978" align="right">02.07.2009</td> | + | <td class="xl7220978" align="right"><small>02.07.2009</small></td> |
- | <td class="xl7320978" align="right">16:08</td> | + | <td class="xl7320978" align="right"><small>16:08</small></td> |
- | <td class="xl7120978" align="right">395.13</td> | + | <td class="xl7120978" align="right"><small>395.13</small></td> |
- | <td class="xl7120978" align="right">7.903</td> | + | <td class="xl7120978" align="right"><small>7.903</small></td> |
- | <td class="xl7120978" align="right">4.201</td> | + | <td class="xl7120978" align="right"><small>4.201</small></td> |
- | <td class="xl7120978" align="right">1.88</td> | + | <td class="xl7120978" align="right"><small>1.88</small></td> |
- | <td class="xl7420978" align="right">2.1</td> | + | <td class="xl7420978" align="right"><small>2.1</small></td> |
| </tr> | | </tr> |
| <tr style="display: none;" height="0"> | | <tr style="display: none;" height="0"> |
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| <li>PCR of the CAT-Vektor (258) with new dNTP-Mix and a | | <li>PCR of the CAT-Vektor (258) with new dNTP-Mix and a |
| anealing-gradient from 50°C to 65°C -> bands at | | anealing-gradient from 50°C to 65°C -> bands at |
- | every temperature with expected size | + | every temperature with expected size </li> |
- | </li>
| + | |
| </ul> | | </ul> |
| <table border="1"> | | <table border="1"> |
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| </table> | | </table> |
| <ul> | | <ul> |
- | <li>PCR Program | + | <li>PCR Program </li> |
- | </li>
| + | |
| </ul> | | </ul> |
| <table border="1"> | | <table border="1"> |
| <caption></caption><tbody> | | <caption></caption><tbody> |
| <tr> | | <tr> |
- | <td align="center">Step | + | <td align="center"><small>Step </small></td> |
- | </td>
| + | <td align="center"><small>Condition </small></td> |
- | <td align="center">Condition | + | <td align="center"><small>Time </small></td> |
- | </td>
| + | |
- | <td align="center">Time | + | |
- | </td>
| + | |
| </tr> | | </tr> |
| <tr> | | <tr> |
- | <td align="center">1 | + | <td align="center"><small>1 </small></td> |
- | </td>
| + | <td align="center"><small>94℃ </small></td> |
- | <td align="center">94℃ | + | <td align="center"><small>4min </small></td> |
- | </td>
| + | |
- | <td align="center">4min | + | |
- | </td>
| + | |
| </tr> | | </tr> |
| <tr> | | <tr> |
- | <td align="center">2 | + | <td align="center"><small>2 </small></td> |
- | </td>
| + | <td align="center"><small>94℃ </small></td> |
- | <td align="center">94℃ | + | <td align="center"><small>1min </small></td> |
- | </td>
| + | |
- | <td align="center">1min | + | |
- | </td>
| + | |
| </tr> | | </tr> |
| <tr> | | <tr> |
- | <td align="center">3 | + | <td align="center"><small>3 </small></td> |
- | </td>
| + | <td align="center"><small>50-65℃ </small></td> |
- | <td align="center">50-65℃ | + | <td align="center"><small>30sec </small></td> |
- | </td>
| + | |
- | <td align="center">30sec | + | |
- | </td>
| + | |
| </tr> | | </tr> |
| <tr> | | <tr> |
- | <td align="center">4 | + | <td align="center"><small>4 </small></td> |
- | </td>
| + | <td align="center"><small>72℃ </small></td> |
- | <td align="center">72℃ | + | <td align="center"><small>1min </small></td> |
- | </td>
| + | |
- | <td align="center">1min | + | |
- | </td>
| + | |
| </tr> | | </tr> |
| <tr> | | <tr> |
- | <td align="center">5 | + | <td align="center"><small>5 </small></td> |
- | </td>
| + | <td align="center"><small>RETURN TO STEP 2 </small></td> |
- | <td align="center">RETURN TO STEP 2 | + | <td align="center"><small>29 cycles </small></td> |
- | </td>
| + | |
- | <td align="center">29 cycles | + | |
- | </td>
| + | |
| </tr> | | </tr> |
| <tr> | | <tr> |
| </tr> | | </tr> |
| <tr> | | <tr> |
- | <td align="center">6 | + | <td align="center"><small>6 </small></td> |
- | </td>
| + | <td align="center"><small>6℃ </small></td> |
- | <td align="center">6℃ | + | <td align="center"><small>HOLD </small></td> |
- | </td>
| + | |
- | <td align="center">HOLD | + | |
- | </td>
| + | |
| </tr> | | </tr> |
| </tbody> | | </tbody> |
Line 358: |
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| src="https://static.igem.org/mediawiki/2009/7/75/Freiburg09_030709_CAT_Gradient_PCR001.jpg" | | src="https://static.igem.org/mediawiki/2009/7/75/Freiburg09_030709_CAT_Gradient_PCR001.jpg" |
| name="Agarose gel of the gradient PCR; Lanes: 50°C to 65°C on the second gel, Marker (100 bp ladder, NEB)" | | name="Agarose gel of the gradient PCR; Lanes: 50°C to 65°C on the second gel, Marker (100 bp ladder, NEB)" |
- | height="170" width="225" /> | + | height="170" width="225" /> </td> |
- | </td>
| + | <td> <img |
- | <td> | + | |
- | <img
| + | |
| src="https://static.igem.org/mediawiki/2009/e/ea/Freiburg09_030709_Verdau_und_CAT_Gradient_PCR001.jpg" | | src="https://static.igem.org/mediawiki/2009/e/ea/Freiburg09_030709_Verdau_und_CAT_Gradient_PCR001.jpg" |
| name="Agarose gel of the gradient PCR; Lanes: Marker (100 bp ladder, NEB), last sample of the gradient PCR, empty lanes, digest of the lab vector #258 with FokI, Marker (1 kb ladder, NEB)" | | name="Agarose gel of the gradient PCR; Lanes: Marker (100 bp ladder, NEB), last sample of the gradient PCR, empty lanes, digest of the lab vector #258 with FokI, Marker (1 kb ladder, NEB)" |
Line 370: |
Line 338: |
| <br /> | | <br /> |
| <ul> | | <ul> |
- | <li>SDS-Gel of the samples from yesterday. | + | <li>SDS-Gel of the samples from yesterday. </li> |
- | </li>
| + | |
| <li>Laemmli buffer mixed and aliquoted -> -20°C</li> | | <li>Laemmli buffer mixed and aliquoted -> -20°C</li> |
| </ul> | | </ul> |
Line 378: |
Line 345: |
| name="SDS-Gel of the expression; Lanes: Marker (prestained broad range 7-200 kDa, T7+Aa 1h..2h..4h, T7+Tt 1h..2h..4h, BL21+Aa 1h..2h..4h" | | name="SDS-Gel of the expression; Lanes: Marker (prestained broad range 7-200 kDa, T7+Aa 1h..2h..4h, T7+Tt 1h..2h..4h, BL21+Aa 1h..2h..4h" |
| height="140" width="225" /><br /> | | height="140" width="225" /><br /> |
- | SDS-Gel of the expression; Lanes: Marker (prestained broad range 7-200 | + | <small>SDS-Gel of the expression; Lanes: Marker (prestained broad |
- | kDa, T7+Aa 1h..2h..4h, T7+Tt 1h..2h..4h, BL21+Aa 1h..2h..4h | + | range 7-200 |
| + | kDa, T7+Aa 1h..2h..4h, T7+Tt 1h..2h..4h, BL21+Aa 1h..2h..4h</small> |
| <h3>06.07.09, 09:00-19:00 Hannes, Laura, Caro, Manuel</h3> | | <h3>06.07.09, 09:00-19:00 Hannes, Laura, Caro, Manuel</h3> |
| <br /> | | <br /> |
Line 388: |
Line 356: |
| µl). We cut the bands, eluted the DNA and got a concentration | | µl). We cut the bands, eluted the DNA and got a concentration |
| from about 100 ng/µl. We used both eluted DNA samples for the | | from about 100 ng/µl. We used both eluted DNA samples for the |
- | restriction digest with HindIII and MfeI (4 hours at 37°C). | + | restriction digest with HindIII and MfeI (4 hours at 37°C). </li> |
- | </li>
| + | |
| <li>After the restriction digest had been incubated for 4 h | | <li>After the restriction digest had been incubated for 4 h |
| (37°C) we purificated it </li> | | (37°C) we purificated it </li> |
| <li>Because of the fact that we eluated with 50ul and not with | | <li>Because of the fact that we eluated with 50ul and not with |
| 30ul (better for the following ligation) we got a concentration of only | | 30ul (better for the following ligation) we got a concentration of only |
- | 87,7ng/ul | + | 87,7ng/ul </li> |
- | </li>
| + | |
| <li> We heat up the sample to 50°C for 1 min | | <li> We heat up the sample to 50°C for 1 min |
- | and measured again. Finally we got a concentration of about 100 ng/ul | + | and measured again. Finally we got a concentration of about 100 ng/ul </li> |
- | </li>
| + | |
| <li> sample Cat gene stored in the -20 °C | | <li> sample Cat gene stored in the -20 °C |
- | freezer | + | freezer </li> |
- | </li>
| + | |
| <li> Inoculation (each 10 ml) for Protein expression | | <li> Inoculation (each 10 ml) for Protein expression |
- | and Glycerolstocks | + | and Glycerolstocks </li> |
- | </li>
| + | |
| <li> <b>Nanodrop data:</b></li> | | <li> <b>Nanodrop data:</b></li> |
| </ul> | | </ul> |
Line 414: |
Line 377: |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl6332240" style="height: 15pt; width: 53pt;" | | <td class="xl6332240" style="height: 15pt; width: 53pt;" |
- | height="20" width="71">Sample | + | height="20" width="71"><small>Sample |
- | ID</td> | + | ID</small></td> |
| <td class="xl6332240" style="width: 48pt;" | | <td class="xl6332240" style="width: 48pt;" |
- | width="64">User ID</td> | + | width="64"><small>User ID</small></td> |
| <td class="xl6332240" style="width: 53pt;" | | <td class="xl6332240" style="width: 53pt;" |
- | width="71">Date<span style=""> </span></td> | + | width="71"><small>Date<span style=""> </span></small></td> |
| <td class="xl6332240" style="width: 48pt;" | | <td class="xl6332240" style="width: 48pt;" |
- | width="64">Time<span style=""> </span></td> | + | width="64"><small>Time<span style=""> </span></small></td> |
| <td class="xl6332240" style="width: 48pt;" | | <td class="xl6332240" style="width: 48pt;" |
- | width="64">ng/ul<span style=""> </span></td> | + | width="64"><small>ng/ul<span style=""> </span></small></td> |
| <td class="xl6332240" style="width: 48pt;" | | <td class="xl6332240" style="width: 48pt;" |
- | width="64">A260<span style=""> </span></td> | + | width="64"><small>A260<span style=""> </span></small></td> |
| <td class="xl6332240" style="width: 48pt;" | | <td class="xl6332240" style="width: 48pt;" |
- | width="64">A280<span style=""> </span></td> | + | width="64"><small>A280<span style=""> </span></small></td> |
| <td class="xl6332240" style="width: 48pt;" | | <td class="xl6332240" style="width: 48pt;" |
- | width="64">260/280<span style=""> </span></td> | + | width="64"><small>260/280<span style=""> </span></small></td> |
| <td class="xl6332240" style="width: 48pt;" | | <td class="xl6332240" style="width: 48pt;" |
- | width="64">260/230<span style=""> </span></td> | + | width="64"><small>260/230<span style=""> </span></small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl6432240" style="height: 15pt;" | | <td class="xl6432240" style="height: 15pt;" |
- | height="20">Cat_Gen</td> | + | height="20"><small>Cat_Gen</small></td> |
- | <td class="xl6432240">FreiGEM</td> | + | <td class="xl6432240"><small>FreiGEM</small></td> |
- | <td class="xl6532240">06.07.2009</td> | + | <td class="xl6532240"><small>06.07.2009</small></td> |
- | <td class="xl6632240">11:28</td> | + | <td class="xl6632240"><small>11:28</small></td> |
- | <td class="xl6432240">29.71</td> | + | <td class="xl6432240"><small>29.71</small></td> |
- | <td class="xl6432240">0.594</td> | + | <td class="xl6432240"><small>0.594</small></td> |
- | <td class="xl6432240">0.292</td> | + | <td class="xl6432240"><small>0.292</small></td> |
- | <td class="xl6432240">2.03</td> | + | <td class="xl6432240"><small>2.03</small></td> |
- | <td class="xl6432240">0.08</td> | + | <td class="xl6432240"><small>0.08</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl6432240" style="height: 15pt;" | | <td class="xl6432240" style="height: 15pt;" |
- | height="20">Cat_Gen</td> | + | height="20"><small>Cat_Gen</small></td> |
- | <td class="xl6432240">FreiGEM</td> | + | <td class="xl6432240"><small>FreiGEM</small></td> |
- | <td class="xl6532240">06.07.2009</td> | + | <td class="xl6532240"><small>06.07.2009</small></td> |
- | <td class="xl6632240">11:30</td> | + | <td class="xl6632240"><small>11:30</small></td> |
- | <td class="xl6432240">36.3</td> | + | <td class="xl6432240"><small>36.3</small></td> |
- | <td class="xl6432240">0.726</td> | + | <td class="xl6432240"><small>0.726</small></td> |
- | <td class="xl6432240">0.35</td> | + | <td class="xl6432240"><small>0.35</small></td> |
- | <td class="xl6432240">2.07</td> | + | <td class="xl6432240"><small>2.07</small></td> |
- | <td class="xl6432240">0.09</td> | + | <td class="xl6432240"><small>0.09</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl6432240" style="height: 15pt;" | | <td class="xl6432240" style="height: 15pt;" |
- | height="20">Cat_Gen</td> | + | height="20"><small>Cat_Gen</small></td> |
- | <td class="xl6432240">FreiGEM</td> | + | <td class="xl6432240"><small>FreiGEM</small></td> |
- | <td class="xl6532240">06.07.2009</td> | + | <td class="xl6532240"><small>06.07.2009</small></td> |
- | <td class="xl6632240">11:42</td> | + | <td class="xl6632240"><small>11:42</small></td> |
- | <td class="xl6432240">54.4</td> | + | <td class="xl6432240"><small>54.4</small></td> |
- | <td class="xl6432240">1.088</td> | + | <td class="xl6432240"><small>1.088</small></td> |
- | <td class="xl6432240">0.622</td> | + | <td class="xl6432240"><small>0.622</small></td> |
- | <td class="xl6432240">1.75</td> | + | <td class="xl6432240"><small>1.75</small></td> |
- | <td class="xl6432240">0.15</td> | + | <td class="xl6432240"><small>0.15</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl6432240" style="height: 15pt;" | | <td class="xl6432240" style="height: 15pt;" |
- | height="20">Cat_Gen</td> | + | height="20"><small>Cat_Gen</small></td> |
- | <td class="xl6432240">FreiGEM</td> | + | <td class="xl6432240"><small>FreiGEM</small></td> |
- | <td class="xl6532240">06.07.2009</td> | + | <td class="xl6532240"><small>06.07.2009</small></td> |
- | <td class="xl6632240">11:43</td> | + | <td class="xl6632240"><small>11:43</small></td> |
- | <td class="xl6432240">41.67</td> | + | <td class="xl6432240"><small>41.67</small></td> |
- | <td class="xl6432240">0.833</td> | + | <td class="xl6432240"><small>0.833</small></td> |
- | <td class="xl6432240">0.43</td> | + | <td class="xl6432240"><small>0.43</small></td> |
- | <td class="xl6432240">1.94</td> | + | <td class="xl6432240"><small>1.94</small></td> |
- | <td class="xl6432240">0.09</td> | + | <td class="xl6432240"><small>0.09</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl6432240" style="height: 15pt;" | | <td class="xl6432240" style="height: 15pt;" |
- | height="20"></td> | + | height="20"><small></small></td> |
- | <td class="xl6432240">FreiGEM</td> | + | <td class="xl6432240"><small>FreiGEM</small></td> |
- | <td class="xl6532240">06.07.2009</td> | + | <td class="xl6532240"><small>06.07.2009</small></td> |
- | <td class="xl6632240">13:24</td> | + | <td class="xl6632240"><small>13:24</small></td> |
- | <td class="xl6432240">100.46</td> | + | <td class="xl6432240"><small>100.46</small></td> |
- | <td class="xl6432240">2.009</td> | + | <td class="xl6432240"><small>2.009</small></td> |
- | <td class="xl6432240">1.085</td> | + | <td class="xl6432240"><small>1.085</small></td> |
- | <td class="xl6432240">1.85</td> | + | <td class="xl6432240"><small>1.85</small></td> |
- | <td class="xl6432240">0.39</td> | + | <td class="xl6432240"><small>0.39</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl6432240" style="height: 15pt;" | | <td class="xl6432240" style="height: 15pt;" |
- | height="20"></td> | + | height="20"><small></small></td> |
- | <td class="xl6432240">FreiGEM</td> | + | <td class="xl6432240"><small>FreiGEM</small></td> |
- | <td class="xl6532240">06.07.2009</td> | + | <td class="xl6532240"><small>06.07.2009</small></td> |
- | <td class="xl6632240">13:25</td> | + | <td class="xl6632240"><small>13:25</small></td> |
- | <td class="xl6432240">102.27</td> | + | <td class="xl6432240"><small>102.27</small></td> |
- | <td class="xl6432240">2.045</td> | + | <td class="xl6432240"><small>2.045</small></td> |
- | <td class="xl6432240">1.097</td> | + | <td class="xl6432240"><small>1.097</small></td> |
- | <td class="xl6432240">1.86</td> | + | <td class="xl6432240"><small>1.86</small></td> |
- | <td class="xl6432240">0.39</td> | + | <td class="xl6432240"><small>0.39</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl6432240" style="height: 15pt;" | | <td class="xl6432240" style="height: 15pt;" |
- | height="20"></td> | + | height="20"><small></small></td> |
- | <td class="xl6432240">FreiGEM</td> | + | <td class="xl6432240"><small>FreiGEM</small></td> |
- | <td class="xl6532240">06.07.2009</td> | + | <td class="xl6532240"><small>06.07.2009</small></td> |
- | <td class="xl6632240">18:18</td> | + | <td class="xl6632240"><small>18:18</small></td> |
- | <td class="xl6432240">87.72</td> | + | <td class="xl6432240"><small>87.72</small></td> |
- | <td class="xl6432240">1.754</td> | + | <td class="xl6432240"><small>1.754</small></td> |
- | <td class="xl6432240">0.933</td> | + | <td class="xl6432240"><small>0.933</small></td> |
- | <td class="xl6432240">1.88</td> | + | <td class="xl6432240"><small>1.88</small></td> |
- | <td class="xl6432240">2.09</td> | + | <td class="xl6432240"><small>2.09</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl6432240" style="height: 15pt;" | | <td class="xl6432240" style="height: 15pt;" |
- | height="20"></td> | + | height="20"><small></small></td> |
- | <td class="xl6432240">FreiGEM</td> | + | <td class="xl6432240"><small>FreiGEM</small></td> |
- | <td class="xl6532240">06.07.2009</td> | + | <td class="xl6532240"><small>06.07.2009</small></td> |
- | <td class="xl6632240">18:25</td> | + | <td class="xl6632240"><small>18:25</small></td> |
- | <td class="xl6432240">92.03</td> | + | <td class="xl6432240"><small>92.03</small></td> |
- | <td class="xl6432240">1.841</td> | + | <td class="xl6432240"><small>1.841</small></td> |
- | <td class="xl6432240">0.937</td> | + | <td class="xl6432240"><small>0.937</small></td> |
- | <td class="xl6432240">1.96</td> | + | <td class="xl6432240"><small>1.96</small></td> |
- | <td class="xl6432240">2.08</td> | + | <td class="xl6432240"><small>2.08</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl6432240" style="height: 15pt;" | | <td class="xl6432240" style="height: 15pt;" |
- | height="20"></td> | + | height="20"><small></small></td> |
- | <td class="xl6432240">FreiGEM</td> | + | <td class="xl6432240"><small>FreiGEM</small></td> |
- | <td class="xl6532240">06.07.2009</td> | + | <td class="xl6532240"><small>06.07.2009</small></td> |
- | <td class="xl6632240">18:28</td> | + | <td class="xl6632240"><small>18:28</small></td> |
- | <td class="xl6432240">103.51</td> | + | <td class="xl6432240"><small>103.51</small></td> |
- | <td class="xl6432240">2.07</td> | + | <td class="xl6432240"><small>2.07</small></td> |
- | <td class="xl6432240">1.226</td> | + | <td class="xl6432240"><small>1.226</small></td> |
- | <td class="xl6432240">1.69</td> | + | <td class="xl6432240"><small>1.69</small></td> |
- | <td class="xl6432240">1.03</td> | + | <td class="xl6432240"><small>1.03</small></td> |
| </tr> | | </tr> |
| <tr style="display: none;" height="0"> | | <tr style="display: none;" height="0"> |
Line 560: |
Line 523: |
| <li>digestion of the pMal-Vektor with HindIII and MfeI (both | | <li>digestion of the pMal-Vektor with HindIII and MfeI (both |
| from the lab freezer), didn't work. Maybe the MfeI enzyme was bad | | from the lab freezer), didn't work. Maybe the MfeI enzyme was bad |
- | (expired 2005) | + | (expired 2005) </li> |
- | </li>
| + | |
| <li>started expression of AGO at 20°C. Took samples at | | <li>started expression of AGO at 20°C. Took samples at |
| 0, 2, 4 and 8 hours centrifuged them, decanted the supernatant and | | 0, 2, 4 and 8 hours centrifuged them, decanted the supernatant and |
- | stored the pellets at -20°C in the box for Sascha. | + | stored the pellets at -20°C in the box for Sascha. </li> |
- | </li>
| + | <li>inoculation of pMal-Vektor from the lb-amp plates </li> |
- | <li>inoculation of pMal-Vektor from the lb-amp plates | + | <li>we did lb-chloramphenicol plates with and without IPTG </li> |
- | </li>
| + | |
- | <li>we did lb-chloramphenicol plates with and without IPTG | + | |
- | </li>
| + | |
| <li><b>Nanodrop data</b></li> | | <li><b>Nanodrop data</b></li> |
| </ul> | | </ul> |
Line 579: |
Line 538: |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl6318459" style="height: 15pt; width: 68pt;" | | <td class="xl6318459" style="height: 15pt; width: 68pt;" |
- | height="20" width="90">Sample | + | height="20" width="90"><small>Sample |
- | ID</td> | + | ID</small></td> |
| <td class="xl6318459" style="width: 48pt;" | | <td class="xl6318459" style="width: 48pt;" |
- | width="64">User ID</td> | + | width="64"><small>User ID</small></td> |
| <td class="xl6318459" style="width: 53pt;" | | <td class="xl6318459" style="width: 53pt;" |
- | width="71">Date<span style=""> </span></td> | + | width="71"><small>Date<span style=""> </span></small></td> |
| <td class="xl6318459" style="width: 48pt;" | | <td class="xl6318459" style="width: 48pt;" |
- | width="64">Time<span style=""> </span></td> | + | width="64"><small>Time<span style=""> </span></small></td> |
| <td class="xl6318459" style="width: 48pt;" | | <td class="xl6318459" style="width: 48pt;" |
- | width="64">ng/ul<span style=""> </span></td> | + | width="64"><small>ng/ul<span style=""> </span></small></td> |
| <td class="xl6318459" style="width: 48pt;" | | <td class="xl6318459" style="width: 48pt;" |
- | width="64">A260<span style=""> </span></td> | + | width="64"><small>A260<span style=""> </span></small></td> |
| <td class="xl6318459" style="width: 48pt;" | | <td class="xl6318459" style="width: 48pt;" |
- | width="64">A280<span style=""> </span></td> | + | width="64"><small>A280<span style=""> </span></small></td> |
| <td class="xl6318459" style="width: 48pt;" | | <td class="xl6318459" style="width: 48pt;" |
- | width="64">260/280<span style=""> </span></td> | + | width="64"><small>260/280<span style=""> </span></small></td> |
| <td class="xl6318459" style="width: 48pt;" | | <td class="xl6318459" style="width: 48pt;" |
- | width="64">260/230<span style=""> </span></td> | + | width="64"><small>260/230<span style=""> </span></small></td> |
| <td class="xl6318459" style="width: 48pt;" | | <td class="xl6318459" style="width: 48pt;" |
- | width="64">340 raw</td> | + | width="64"><small>340 raw</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl6718459" style="height: 15pt;" | | <td class="xl6718459" style="height: 15pt;" |
- | height="20">pMal 1</td> | + | height="20"><small>pMal 1</small></td> |
- | <td class="xl6418459">FreiGEM</td> | + | <td class="xl6418459"><small>FreiGEM</small></td> |
- | <td class="xl6518459">07.07.2009</td> | + | <td class="xl6518459"><small>07.07.2009</small></td> |
- | <td class="xl6618459">09:32</td> | + | <td class="xl6618459"><small>09:32</small></td> |
- | <td class="xl6418459">142.36</td> | + | <td class="xl6418459"><small>142.36</small></td> |
- | <td class="xl6418459">2.847</td> | + | <td class="xl6418459"><small>2.847</small></td> |
- | <td class="xl6418459">1.496</td> | + | <td class="xl6418459"><small>1.496</small></td> |
- | <td class="xl6418459">1.9</td> | + | <td class="xl6418459"><small>1.9</small></td> |
- | <td class="xl6418459">2.02</td> | + | <td class="xl6418459"><small>2.02</small></td> |
- | <td class="xl6418459">0.032</td> | + | <td class="xl6418459"><small>0.032</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl6718459" style="height: 15pt;" | | <td class="xl6718459" style="height: 15pt;" |
- | height="20">pMal 1</td> | + | height="20"><small>pMal 1</small></td> |
- | <td class="xl6418459">FreiGEM</td> | + | <td class="xl6418459"><small>FreiGEM</small></td> |
- | <td class="xl6518459">07.07.2009</td> | + | <td class="xl6518459"><small>07.07.2009</small></td> |
- | <td class="xl6618459">09:34</td> | + | <td class="xl6618459"><small>09:34</small></td> |
- | <td class="xl6418459">141.31</td> | + | <td class="xl6418459"><small>141.31</small></td> |
- | <td class="xl6418459">2.826</td> | + | <td class="xl6418459"><small>2.826</small></td> |
- | <td class="xl6418459">1.478</td> | + | <td class="xl6418459"><small>1.478</small></td> |
- | <td class="xl6418459">1.91</td> | + | <td class="xl6418459"><small>1.91</small></td> |
- | <td class="xl6418459">2.34</td> | + | <td class="xl6418459"><small>2.34</small></td> |
- | <td class="xl6418459">0.08</td> | + | <td class="xl6418459"><small>0.08</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl6718459" style="height: 15pt;" | | <td class="xl6718459" style="height: 15pt;" |
- | height="20">pMal 2</td> | + | height="20"><small>pMal 2</small></td> |
- | <td class="xl6418459">FreiGEM</td> | + | <td class="xl6418459"><small>FreiGEM</small></td> |
- | <td class="xl6518459">07.07.2009</td> | + | <td class="xl6518459"><small>07.07.2009</small></td> |
- | <td class="xl6618459">09:34</td> | + | <td class="xl6618459"><small>09:34</small></td> |
- | <td class="xl6418459">142.46</td> | + | <td class="xl6418459"><small>142.46</small></td> |
- | <td class="xl6418459">2.849</td> | + | <td class="xl6418459"><small>2.849</small></td> |
- | <td class="xl6418459">1.495</td> | + | <td class="xl6418459"><small>1.495</small></td> |
- | <td class="xl6418459">1.91</td> | + | <td class="xl6418459"><small>1.91</small></td> |
- | <td class="xl6418459">1.96</td> | + | <td class="xl6418459"><small>1.96</small></td> |
- | <td class="xl6418459">0.013</td> | + | <td class="xl6418459"><small>0.013</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl6718459" style="height: 15pt;" | | <td class="xl6718459" style="height: 15pt;" |
- | height="20">pMal 2</td> | + | height="20"><small>pMal 2</small></td> |
- | <td class="xl6418459">FreiGEM</td> | + | <td class="xl6418459"><small>FreiGEM</small></td> |
- | <td class="xl6518459">07.07.2009</td> | + | <td class="xl6518459"><small>07.07.2009</small></td> |
- | <td class="xl6618459">09:35</td> | + | <td class="xl6618459"><small>09:35</small></td> |
- | <td class="xl6418459">148.44</td> | + | <td class="xl6418459"><small>148.44</small></td> |
- | <td class="xl6418459">2.969</td> | + | <td class="xl6418459"><small>2.969</small></td> |
- | <td class="xl6418459">1.535</td> | + | <td class="xl6418459"><small>1.535</small></td> |
- | <td class="xl6418459">1.93</td> | + | <td class="xl6418459"><small>1.93</small></td> |
- | <td class="xl6418459">1.92</td> | + | <td class="xl6418459"><small>1.92</small></td> |
- | <td class="xl6418459">0.035</td> | + | <td class="xl6418459"><small>0.035</small></td> |
| </tr> | | </tr> |
| <tr style="display: none;" height="0"> | | <tr style="display: none;" height="0"> |
Line 670: |
Line 629: |
| <ul> | | <ul> |
| <li>new digest of pMAL expression vector and CAT gene with MfeI | | <li>new digest of pMAL expression vector and CAT gene with MfeI |
- | and HindIII for four hours | + | and HindIII for four hours </li> |
- | </li>
| + | |
| <li>agarose gel with these two samples -> bands of CAT | | <li>agarose gel with these two samples -> bands of CAT |
| gene (probably cut) around 700 bp and pMAL vector around 5kb and MalE | | gene (probably cut) around 700 bp and pMAL vector around 5kb and MalE |
- | gene around 1,4kb could be seen, bands of CAT and vector were cut out | + | gene around 1,4kb could be seen, bands of CAT and vector were cut out </li> |
- | </li>
| + | |
| <li>gel extraction of the bands</li> | | <li>gel extraction of the bands</li> |
| <ul> | | <ul> |
Line 682: |
Line 639: |
| </ul> | | </ul> |
| <li>ligation with the quick ligase (total volume of | | <li>ligation with the quick ligase (total volume of |
- | 21µl) | + | 21µl) </li> |
- | </li>
| + | |
| <li>transformation of 10 µl of ligated vector with | | <li>transformation of 10 µl of ligated vector with |
| new insert into 100 µl competent cells XBL -> plating | | new insert into 100 µl competent cells XBL -> plating |
Line 690: |
Line 646: |
| respectively -> 37°C room over night -> | | respectively -> 37°C room over night -> |
| inoculation can be done tomorrow during the day when the cultures seem | | inoculation can be done tomorrow during the day when the cultures seem |
- | to be grown well (after 14 h minimum) | + | to be grown well (after 14 h minimum) </li> |
- | </li>
| + | <li><b>Nanodrop data</b> </li> |
- | <li><b>Nanodrop data</b> | + | <li>Protein A-280 </li> |
- | </li>
| + | |
- | <li>Protein A-280 | + | |
- | </li>
| + | |
| </ul> | | </ul> |
| <table | | <table |
Line 704: |
Line 657: |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl1517775" style="height: 15pt; width: 84pt;" | | <td class="xl1517775" style="height: 15pt; width: 84pt;" |
- | height="20" width="112">Sample | + | height="20" width="112"><small>Sample |
- | ID</td> | + | ID</small></td> |
| <td class="xl1517775" style="width: 48pt;" | | <td class="xl1517775" style="width: 48pt;" |
- | width="64">User ID</td> | + | width="64"><small>User ID</small></td> |
| <td class="xl1517775" style="width: 53pt;" | | <td class="xl1517775" style="width: 53pt;" |
- | width="71">Date<span style=""> </span></td> | + | width="71"><small>Date<span style=""> </span></small></td> |
| <td class="xl1517775" style="width: 48pt;" | | <td class="xl1517775" style="width: 48pt;" |
- | width="64">Time<span style=""> </span></td> | + | width="64"><small>Time<span style=""> </span></small></td> |
| <td class="xl1517775" style="width: 48pt;" | | <td class="xl1517775" style="width: 48pt;" |
- | width="64">mg/ml<span style=""> </span></td> | + | width="64"><small>mg/ml<span style=""> </span></small></td> |
| <td class="xl1517775" style="width: 48pt;" | | <td class="xl1517775" style="width: 48pt;" |
- | width="64">260/280<span style=""> </span></td> | + | width="64"><small>260/280<span style=""> </span></small></td> |
| <td class="xl1517775" style="width: 66pt;" | | <td class="xl1517775" style="width: 66pt;" |
- | width="88">A280 10mm</td> | + | width="88"><small>A280 10mm</small></td> |
| <td class="xl1517775" style="width: 71pt;" | | <td class="xl1517775" style="width: 71pt;" |
- | width="94">E 1%</td> | + | width="94"><small>E 1%</small></td> |
| <td class="xl1517775" style="width: 95pt;" | | <td class="xl1517775" style="width: 95pt;" |
- | width="126">Ext. Coeff x10e3</td> | + | width="126"><small>Ext. Coeff x10e3</small></td> |
| <td class="xl1517775" style="width: 73pt;" | | <td class="xl1517775" style="width: 73pt;" |
- | width="97">Mol. Wt. kDa</td> | + | width="97"><small>Mol. Wt. kDa</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl1517775" style="height: 15pt;" | | <td class="xl1517775" style="height: 15pt;" |
- | height="20">T7 Aa 1</td> | + | height="20"><small>T7 Aa 1</small></td> |
- | <td class="xl1517775">FreiGEM</td> | + | <td class="xl1517775"><small>FreiGEM</small></td> |
- | <td class="xl6317775" align="right">08.07.2009</td> | + | <td class="xl6317775" align="right"><small>08.07.2009</small></td> |
- | <td class="xl6417775" align="right">19:21</td> | + | <td class="xl6417775" align="right"><small>19:21</small></td> |
- | <td class="xl1517775" align="right">0.12</td> | + | <td class="xl1517775" align="right"><small>0.12</small></td> |
- | <td class="xl1517775" align="right">1.39</td> | + | <td class="xl1517775" align="right"><small>1.39</small></td> |
- | <td class="xl1517775" align="right">0.115</td> | + | <td class="xl1517775" align="right"><small>0.115</small></td> |
- | <td class="xl1517775" align="right">10</td> | + | <td class="xl1517775" align="right"><small>10</small></td> |
- | <td class="xl1517775">NaN</td> | + | <td class="xl1517775"><small>NaN</small></td> |
- | <td class="xl1517775">NaN</td> | + | <td class="xl1517775"><small>NaN</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl1517775" style="height: 15pt;" | | <td class="xl1517775" style="height: 15pt;" |
- | height="20">T7 Aa 2</td> | + | height="20"><small>T7 Aa 2</small></td> |
- | <td class="xl1517775">FreiGEM</td> | + | <td class="xl1517775"><small>FreiGEM</small></td> |
- | <td class="xl6317775" align="right">08.07.2009</td> | + | <td class="xl6317775" align="right"><small>08.07.2009</small></td> |
- | <td class="xl6417775" align="right">19:31</td> | + | <td class="xl6417775" align="right"><small>19:31</small></td> |
- | <td class="xl1517775" align="right">0.19</td> | + | <td class="xl1517775" align="right"><small>0.19</small></td> |
- | <td class="xl1517775" align="right">1.35</td> | + | <td class="xl1517775" align="right"><small>1.35</small></td> |
- | <td class="xl1517775" align="right">0.191</td> | + | <td class="xl1517775" align="right"><small>0.191</small></td> |
- | <td class="xl1517775" align="right">10</td> | + | <td class="xl1517775" align="right"><small>10</small></td> |
- | <td class="xl1517775">NaN</td> | + | <td class="xl1517775"><small>NaN</small></td> |
- | <td class="xl1517775">NaN</td> | + | <td class="xl1517775"><small>NaN</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl1517775" style="height: 15pt;" | | <td class="xl1517775" style="height: 15pt;" |
- | height="20">T7 Aa 3</td> | + | height="20"><small>T7 Aa 3</small></td> |
- | <td class="xl1517775">FreiGEM</td> | + | <td class="xl1517775"><small>FreiGEM</small></td> |
- | <td class="xl6317775" align="right">08.07.2009</td> | + | <td class="xl6317775" align="right"><small>08.07.2009</small></td> |
- | <td class="xl6417775" align="right">19:32</td> | + | <td class="xl6417775" align="right"><small>19:32</small></td> |
- | <td class="xl1517775" align="right">1.06</td> | + | <td class="xl1517775" align="right"><small>1.06</small></td> |
- | <td class="xl1517775" align="right">0.69</td> | + | <td class="xl1517775" align="right"><small>0.69</small></td> |
- | <td class="xl1517775" align="right">1.061</td> | + | <td class="xl1517775" align="right"><small>1.061</small></td> |
- | <td class="xl1517775" align="right">10</td> | + | <td class="xl1517775" align="right"><small>10</small></td> |
- | <td class="xl1517775">NaN</td> | + | <td class="xl1517775"><small>NaN</small></td> |
- | <td class="xl1517775">NaN</td> | + | <td class="xl1517775"><small>NaN</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl1517775" style="height: 15pt;" | | <td class="xl1517775" style="height: 15pt;" |
- | height="20">T7 Aa 4</td> | + | height="20"><small>T7 Aa 4</small></td> |
- | <td class="xl1517775">FreiGEM</td> | + | <td class="xl1517775"><small>FreiGEM</small></td> |
- | <td class="xl6317775" align="right">08.07.2009</td> | + | <td class="xl6317775" align="right"><small>08.07.2009</small></td> |
- | <td class="xl6417775" align="right">19:33</td> | + | <td class="xl6417775" align="right"><small>19:33</small></td> |
- | <td class="xl1517775" align="right">0.64</td> | + | <td class="xl1517775" align="right"><small>0.64</small></td> |
- | <td class="xl1517775" align="right">0.64</td> | + | <td class="xl1517775" align="right"><small>0.64</small></td> |
- | <td class="xl1517775" align="right">0.64</td> | + | <td class="xl1517775" align="right"><small>0.64</small></td> |
- | <td class="xl1517775" align="right">10</td> | + | <td class="xl1517775" align="right"><small>10</small></td> |
- | <td class="xl1517775">NaN</td> | + | <td class="xl1517775"><small>NaN</small></td> |
- | <td class="xl1517775">NaN</td> | + | <td class="xl1517775"><small>NaN</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl1517775" style="height: 15pt;" | | <td class="xl1517775" style="height: 15pt;" |
- | height="20">T7 Aa 5</td> | + | height="20"><small>T7 Aa 5</small></td> |
- | <td class="xl1517775">FreiGEM</td> | + | <td class="xl1517775"><small>FreiGEM</small></td> |
- | <td class="xl6317775" align="right">08.07.2009</td> | + | <td class="xl6317775" align="right"><small>08.07.2009</small></td> |
- | <td class="xl6417775" align="right">19:34</td> | + | <td class="xl6417775" align="right"><small>19:34</small></td> |
- | <td class="xl1517775" align="right">0.44</td> | + | <td class="xl1517775" align="right"><small>0.44</small></td> |
- | <td class="xl1517775" align="right">0.73</td> | + | <td class="xl1517775" align="right"><small>0.73</small></td> |
- | <td class="xl1517775" align="right">0.444</td> | + | <td class="xl1517775" align="right"><small>0.444</small></td> |
- | <td class="xl1517775" align="right">10</td> | + | <td class="xl1517775" align="right"><small>10</small></td> |
- | <td class="xl1517775">NaN</td> | + | <td class="xl1517775"><small>NaN</small></td> |
- | <td class="xl1517775">NaN</td> | + | <td class="xl1517775"><small>NaN</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl1517775" style="height: 15pt;" | | <td class="xl1517775" style="height: 15pt;" |
- | height="20">T7 Aa 6</td> | + | height="20"><small>T7 Aa 6</small></td> |
- | <td class="xl1517775">FreiGEM</td> | + | <td class="xl1517775"><small>FreiGEM</small></td> |
- | <td class="xl6317775" align="right">08.07.2009</td> | + | <td class="xl6317775" align="right"><small>08.07.2009</small></td> |
- | <td class="xl6417775" align="right">19:35</td> | + | <td class="xl6417775" align="right"><small>19:35</small></td> |
- | <td class="xl1517775" align="right">0.25</td> | + | <td class="xl1517775" align="right"><small>0.25</small></td> |
- | <td class="xl1517775" align="right">0.61</td> | + | <td class="xl1517775" align="right"><small>0.61</small></td> |
- | <td class="xl1517775" align="right">0.252</td> | + | <td class="xl1517775" align="right"><small>0.252</small></td> |
- | <td class="xl1517775" align="right">10</td> | + | <td class="xl1517775" align="right"><small>10</small></td> |
- | <td class="xl1517775">NaN</td> | + | <td class="xl1517775"><small>NaN</small></td> |
- | <td class="xl1517775">NaN</td> | + | <td class="xl1517775"><small>NaN</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl1517775" style="height: 15pt;" | | <td class="xl1517775" style="height: 15pt;" |
- | height="20">T7 Aa 7</td> | + | height="20"><small>T7 Aa 7</small></td> |
- | <td class="xl1517775">FreiGEM</td> | + | <td class="xl1517775"><small>FreiGEM</small></td> |
- | <td class="xl6317775" align="right">08.07.2009</td> | + | <td class="xl6317775" align="right"><small>08.07.2009</small></td> |
- | <td class="xl6417775" align="right">19:35</td> | + | <td class="xl6417775" align="right"><small>19:35</small></td> |
- | <td class="xl1517775" align="right">0.18</td> | + | <td class="xl1517775" align="right"><small>0.18</small></td> |
- | <td class="xl1517775" align="right">0.77</td> | + | <td class="xl1517775" align="right"><small>0.77</small></td> |
- | <td class="xl1517775" align="right">0.183</td> | + | <td class="xl1517775" align="right"><small>0.183</small></td> |
- | <td class="xl1517775" align="right">10</td> | + | <td class="xl1517775" align="right"><small>10</small></td> |
- | <td class="xl1517775">NaN</td> | + | <td class="xl1517775"><small>NaN</small></td> |
- | <td class="xl1517775">NaN</td> | + | <td class="xl1517775"><small>NaN</small></td> |
| </tr> | | </tr> |
| <tr style="display: none;" height="0"> | | <tr style="display: none;" height="0"> |
Line 831: |
Line 784: |
| </table> | | </table> |
| <ul> | | <ul> |
- | <li> Nucleic acid | + | <li> Nucleic acid </li> |
- | </li>
| + | |
| </ul> | | </ul> |
| <table | | <table |
Line 841: |
Line 793: |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl6522952" style="height: 15pt; width: 84pt;" | | <td class="xl6522952" style="height: 15pt; width: 84pt;" |
- | height="20" width="112">Sample | + | height="20" width="112"><small>Sample |
- | ID</td> | + | ID</small></td> |
| <td class="xl6522952" style="width: 48pt;" | | <td class="xl6522952" style="width: 48pt;" |
- | width="64">User ID</td> | + | width="64"><small>User ID</small></td> |
| <td class="xl6522952" style="width: 53pt;" | | <td class="xl6522952" style="width: 53pt;" |
- | width="71">Date<span style=""> </span></td> | + | width="71"><small>Date<span style=""> </span></small></td> |
| <td class="xl6522952" style="width: 48pt;" | | <td class="xl6522952" style="width: 48pt;" |
- | width="64">Time<span style=""> </span></td> | + | width="64"><small>Time<span style=""> </span></small></td> |
| <td class="xl6522952" style="width: 48pt;" | | <td class="xl6522952" style="width: 48pt;" |
- | width="64">ng/ul<span style=""> </span></td> | + | width="64"><small>ng/ul<span style=""> </span></small></td> |
| <td class="xl6522952" style="width: 48pt;" | | <td class="xl6522952" style="width: 48pt;" |
- | width="64">A260<span style=""> </span></td> | + | width="64"><small>A260<span style=""> </span></small></td> |
| <td class="xl6522952" style="width: 48pt;" | | <td class="xl6522952" style="width: 48pt;" |
- | width="64">A280<span style=""> </span></td> | + | width="64"><small>A280<span style=""> </span></small></td> |
| <td class="xl6522952" style="width: 48pt;" | | <td class="xl6522952" style="width: 48pt;" |
- | width="64">260/280<span style=""> </span></td> | + | width="64"><small>260/280<span style=""> </span></small></td> |
| <td class="xl6522952" style="width: 48pt;" | | <td class="xl6522952" style="width: 48pt;" |
- | width="64">260/230<span style=""> </span></td> | + | width="64"><small>260/230<span style=""> </span></small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl1522952" style="height: 15pt;" | | <td class="xl1522952" style="height: 15pt;" |
- | height="20">T7 Aa 1</td> | + | height="20"><small>T7 Aa 1</small></td> |
- | <td class="xl1522952">FreiGEM</td> | + | <td class="xl1522952"><small>FreiGEM</small></td> |
- | <td class="xl6322952" align="right">08.07.2009</td> | + | <td class="xl6322952" align="right"><small>08.07.2009</small></td> |
- | <td class="xl6422952" align="right">18:12</td> | + | <td class="xl6422952" align="right"><small>18:12</small></td> |
- | <td class="xl1522952" align="right">18.83</td> | + | <td class="xl1522952" align="right"><small>18.83</small></td> |
- | <td class="xl1522952" align="right">0.377</td> | + | <td class="xl1522952" align="right"><small>0.377</small></td> |
- | <td class="xl1522952" align="right">0.183</td> | + | <td class="xl1522952" align="right"><small>0.183</small></td> |
- | <td class="xl1522952" align="right">2.06</td> | + | <td class="xl1522952" align="right"><small>2.06</small></td> |
- | <td class="xl1522952" align="right">0.05</td> | + | <td class="xl1522952" align="right"><small>0.05</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl1522952" style="height: 15pt;" | | <td class="xl1522952" style="height: 15pt;" |
- | height="20">T7 Aa 2</td> | + | height="20"><small>T7 Aa 2</small></td> |
- | <td class="xl1522952">FreiGEM</td> | + | <td class="xl1522952"><small>FreiGEM</small></td> |
- | <td class="xl6322952" align="right">08.07.2009</td> | + | <td class="xl6322952" align="right"><small>08.07.2009</small></td> |
- | <td class="xl6422952" align="right">18:13</td> | + | <td class="xl6422952" align="right"><small>18:13</small></td> |
- | <td class="xl1522952" align="right">29.81</td> | + | <td class="xl1522952" align="right"><small>29.81</small></td> |
- | <td class="xl1522952" align="right">0.596</td> | + | <td class="xl1522952" align="right"><small>0.596</small></td> |
- | <td class="xl1522952" align="right">0.316</td> | + | <td class="xl1522952" align="right"><small>0.316</small></td> |
- | <td class="xl1522952" align="right">1.89</td> | + | <td class="xl1522952" align="right"><small>1.89</small></td> |
- | <td class="xl1522952" align="right">0.16</td> | + | <td class="xl1522952" align="right"><small>0.16</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl1522952" style="height: 15pt;" | | <td class="xl1522952" style="height: 15pt;" |
- | height="20">T7 Aa 3</td> | + | height="20"><small>T7 Aa 3</small></td> |
- | <td class="xl1522952">FreiGEM</td> | + | <td class="xl1522952"><small>FreiGEM</small></td> |
- | <td class="xl6322952" align="right">08.07.2009</td> | + | <td class="xl6322952" align="right"><small>08.07.2009</small></td> |
- | <td class="xl6422952" align="right">18:20</td> | + | <td class="xl6422952" align="right"><small>18:20</small></td> |
- | <td class="xl1522952" align="right">14.91</td> | + | <td class="xl1522952" align="right"><small>14.91</small></td> |
- | <td class="xl1522952" align="right">0.298</td> | + | <td class="xl1522952" align="right"><small>0.298</small></td> |
- | <td class="xl1522952" align="right">0.119</td> | + | <td class="xl1522952" align="right"><small>0.119</small></td> |
- | <td class="xl1522952" align="right">2.52</td> | + | <td class="xl1522952" align="right"><small>2.52</small></td> |
- | <td class="xl1522952" align="right">0.05</td> | + | <td class="xl1522952" align="right"><small>0.05</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl1522952" style="height: 15pt;" | | <td class="xl1522952" style="height: 15pt;" |
- | height="20">T7 Aa 4</td> | + | height="20"><small>T7 Aa 4</small></td> |
- | <td class="xl1522952">FreiGEM</td> | + | <td class="xl1522952"><small>FreiGEM</small></td> |
- | <td class="xl6322952" align="right">08.07.2009</td> | + | <td class="xl6322952" align="right"><small>08.07.2009</small></td> |
- | <td class="xl6422952" align="right">18:21</td> | + | <td class="xl6422952" align="right"><small>18:21</small></td> |
- | <td class="xl1522952" align="right">48.67</td> | + | <td class="xl1522952" align="right"><small>48.67</small></td> |
- | <td class="xl1522952" align="right">0.973</td> | + | <td class="xl1522952" align="right"><small>0.973</small></td> |
- | <td class="xl1522952" align="right">0.546</td> | + | <td class="xl1522952" align="right"><small>0.546</small></td> |
- | <td class="xl1522952" align="right">1.78</td> | + | <td class="xl1522952" align="right"><small>1.78</small></td> |
- | <td class="xl1522952" align="right">0.11</td> | + | <td class="xl1522952" align="right"><small>0.11</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl1522952" style="height: 15pt;" | | <td class="xl1522952" style="height: 15pt;" |
- | height="20">T7 Aa 5</td> | + | height="20"><small>T7 Aa 5</small></td> |
- | <td class="xl1522952">FreiGEM</td> | + | <td class="xl1522952"><small>FreiGEM</small></td> |
- | <td class="xl6322952" align="right">08.07.2009</td> | + | <td class="xl6322952" align="right"><small>08.07.2009</small></td> |
- | <td class="xl6422952" align="right">18:22</td> | + | <td class="xl6422952" align="right"><small>18:22</small></td> |
- | <td class="xl1522952" align="right">26.32</td> | + | <td class="xl1522952" align="right"><small>26.32</small></td> |
- | <td class="xl1522952" align="right">0.526</td> | + | <td class="xl1522952" align="right"><small>0.526</small></td> |
- | <td class="xl1522952" align="right">0.271</td> | + | <td class="xl1522952" align="right"><small>0.271</small></td> |
- | <td class="xl1522952" align="right">1.94</td> | + | <td class="xl1522952" align="right"><small>1.94</small></td> |
- | <td class="xl1522952" align="right">0.06</td> | + | <td class="xl1522952" align="right"><small>0.06</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl1522952" style="height: 15pt;" | | <td class="xl1522952" style="height: 15pt;" |
- | height="20">T7 Aa 6</td> | + | height="20"><small>T7 Aa 6</small></td> |
- | <td class="xl1522952">FreiGEM</td> | + | <td class="xl1522952"><small>FreiGEM</small></td> |
- | <td class="xl6322952" align="right">08.07.2009</td> | + | <td class="xl6322952" align="right"><small>08.07.2009</small></td> |
- | <td class="xl6422952" align="right">18:23</td> | + | <td class="xl6422952" align="right"><small>18:23</small></td> |
- | <td class="xl1522952" align="right">20.8</td> | + | <td class="xl1522952" align="right"><small>20.8</small></td> |
- | <td class="xl1522952" align="right">0.416</td> | + | <td class="xl1522952" align="right"><small>0.416</small></td> |
- | <td class="xl1522952" align="right">0.184</td> | + | <td class="xl1522952" align="right"><small>0.184</small></td> |
- | <td class="xl1522952" align="right">2.26</td> | + | <td class="xl1522952" align="right"><small>2.26</small></td> |
- | <td class="xl1522952" align="right">0.05</td> | + | <td class="xl1522952" align="right"><small>0.05</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl1522952" style="height: 15pt;" | | <td class="xl1522952" style="height: 15pt;" |
- | height="20">T7 Aa 7</td> | + | height="20"><small>T7 Aa 7</small></td> |
- | <td class="xl1522952">FreiGEM</td> | + | <td class="xl1522952"><small>FreiGEM</small></td> |
- | <td class="xl6322952" align="right">08.07.2009</td> | + | <td class="xl6322952" align="right"><small>08.07.2009</small></td> |
- | <td class="xl6422952" align="right">18:24</td> | + | <td class="xl6422952" align="right"><small>18:24</small></td> |
- | <td class="xl1522952" align="right">83.81</td> | + | <td class="xl1522952" align="right"><small>83.81</small></td> |
- | <td class="xl1522952" align="right">1.676</td> | + | <td class="xl1522952" align="right"><small>1.676</small></td> |
- | <td class="xl1522952" align="right">1.011</td> | + | <td class="xl1522952" align="right"><small>1.011</small></td> |
- | <td class="xl1522952" align="right">1.66</td> | + | <td class="xl1522952" align="right"><small>1.66</small></td> |
- | <td class="xl1522952" align="right">0.28</td> | + | <td class="xl1522952" align="right"><small>0.28</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl1522952" style="height: 15pt;" | | <td class="xl1522952" style="height: 15pt;" |
- | height="20">T7 Aa 8</td> | + | height="20"><small>T7 Aa 8</small></td> |
- | <td class="xl1522952">FreiGEM</td> | + | <td class="xl1522952"><small>FreiGEM</small></td> |
- | <td class="xl6322952" align="right">08.07.2009</td> | + | <td class="xl6322952" align="right"><small>08.07.2009</small></td> |
- | <td class="xl6422952" align="right">18:24</td> | + | <td class="xl6422952" align="right"><small>18:24</small></td> |
- | <td class="xl1522952" align="right">21.16</td> | + | <td class="xl1522952" align="right"><small>21.16</small></td> |
- | <td class="xl1522952" align="right">0.423</td> | + | <td class="xl1522952" align="right"><small>0.423</small></td> |
- | <td class="xl1522952" align="right">0.196</td> | + | <td class="xl1522952" align="right"><small>0.196</small></td> |
- | <td class="xl1522952" align="right">2.16</td> | + | <td class="xl1522952" align="right"><small>2.16</small></td> |
- | <td class="xl1522952" align="right">0.11</td> | + | <td class="xl1522952" align="right"><small>0.11</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl1522952" style="height: 15pt;" | | <td class="xl1522952" style="height: 15pt;" |
- | height="20">T7 Aa 9</td> | + | height="20"><small>T7 Aa 9</small></td> |
- | <td class="xl1522952">FreiGEM</td> | + | <td class="xl1522952"><small>FreiGEM</small></td> |
- | <td class="xl6322952" align="right">08.07.2009</td> | + | <td class="xl6322952" align="right"><small>08.07.2009</small></td> |
- | <td class="xl6422952" align="right">18:25</td> | + | <td class="xl6422952" align="right"><small>18:25</small></td> |
- | <td class="xl1522952" align="right">23.61</td> | + | <td class="xl1522952" align="right"><small>23.61</small></td> |
- | <td class="xl1522952" align="right">0.472</td> | + | <td class="xl1522952" align="right"><small>0.472</small></td> |
- | <td class="xl1522952" align="right">0.223</td> | + | <td class="xl1522952" align="right"><small>0.223</small></td> |
- | <td class="xl1522952" align="right">2.12</td> | + | <td class="xl1522952" align="right"><small>2.12</small></td> |
- | <td class="xl1522952" align="right">0.11</td> | + | <td class="xl1522952" align="right"><small>0.11</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl1522952" style="height: 15pt;" | | <td class="xl1522952" style="height: 15pt;" |
- | height="20">T7 Aa 10</td> | + | height="20"><small>T7 Aa 10</small></td> |
- | <td class="xl1522952">FreiGEM</td> | + | <td class="xl1522952"><small>FreiGEM</small></td> |
- | <td class="xl6322952" align="right">08.07.2009</td> | + | <td class="xl6322952" align="right"><small>08.07.2009</small></td> |
- | <td class="xl6422952" align="right">19:02</td> | + | <td class="xl6422952" align="right"><small>19:02</small></td> |
- | <td class="xl1522952" align="right">1.33</td> | + | <td class="xl1522952" align="right"><small>1.33</small></td> |
- | <td class="xl1522952" align="right">0.027</td> | + | <td class="xl1522952" align="right"><small>0.027</small></td> |
- | <td class="xl1522952" align="right">0.018</td> | + | <td class="xl1522952" align="right"><small>0.018</small></td> |
- | <td class="xl1522952" align="right">1.45</td> | + | <td class="xl1522952" align="right"><small>1.45</small></td> |
- | <td class="xl1522952" align="right">0.07</td> | + | <td class="xl1522952" align="right"><small>0.07</small></td> |
| </tr> | | </tr> |
| <tr style="display: none;" height="0"> | | <tr style="display: none;" height="0"> |
Line 1,003: |
Line 955: |
| name="Westernblot of the AGOexpression; Lanes: empty lane, Tt in BL21 DE3 at 8h and 0h, Aa in BL21 DE3 at 8h and 0h, Tt in T7 at 8h and 0h, Aa in T7 at 8h and 0h 500µl culture each" | | name="Westernblot of the AGOexpression; Lanes: empty lane, Tt in BL21 DE3 at 8h and 0h, Aa in BL21 DE3 at 8h and 0h, Tt in T7 at 8h and 0h, Aa in T7 at 8h and 0h 500µl culture each" |
| height="150" width="200" /><br /> | | height="150" width="200" /><br /> |
- | Westernblot of the AGOexpression; Lanes: empty lane, Tt in BL21 DE3 at | + | <small>Westernblot of the AGOexpression; Lanes: empty |
| + | lane, Tt in BL21 DE3 at |
| 8h and 0h, Aa in BL21 DE3 at 8h and 0h, Tt in T7 at 8h and 0h, Aa in T7 | | 8h and 0h, Aa in BL21 DE3 at 8h and 0h, Tt in T7 at 8h and 0h, Aa in T7 |
| at 8h and 0h 500µl culture each. | | at 8h and 0h 500µl culture each. |
- | <br /> | + | </small><br /> |
| <ul> | | <ul> |
| <li>SDS page, Coomassie staining</li> | | <li>SDS page, Coomassie staining</li> |
Line 1,014: |
Line 967: |
| name="SDS page of the AGOexpression; Lanes: Protein marker NEB, Aa in BL21 de3 waste fraction, fraction 1, fraction 2, fraction 3, Aa in T7 waste fraction, fraction 1, fraction 2, fraction 3, fraction 4" | | name="SDS page of the AGOexpression; Lanes: Protein marker NEB, Aa in BL21 de3 waste fraction, fraction 1, fraction 2, fraction 3, Aa in T7 waste fraction, fraction 1, fraction 2, fraction 3, fraction 4" |
| height="170" width="250" /><br /> | | height="170" width="250" /><br /> |
- | SDS page of the AGOexpression; Lanes: Protein marker NEB, Aa in BL21 | + | <small>SDS page of the AGOexpression; Lanes: Protein marker NEB, |
| + | Aa in BL21 |
| de3 waste fraction, fraction 1, fraction 2, fraction 3, Aa in T7 waste | | de3 waste fraction, fraction 1, fraction 2, fraction 3, Aa in T7 waste |
| fraction, fraction 1, fraction 2, fraction 3, fraction 4 | | fraction, fraction 1, fraction 2, fraction 3, fraction 4 |
- | <br /> | + | </small><br /> |
| <ul> | | <ul> |
| <li> Our AGO proteins are at about 80 kDa. Aa 83,6 kDa | | <li> Our AGO proteins are at about 80 kDa. Aa 83,6 kDa |
- | and Tt 76,7 kDa | + | and Tt 76,7 kDa </li> |
- | </li>
| + | |
| <li>His-Tag purification via Ni-NTA column and concentration | | <li>His-Tag purification via Ni-NTA column and concentration |
| measurement at the Nanodrop, Volume of peristaltic pump 3,5ml, | | measurement at the Nanodrop, Volume of peristaltic pump 3,5ml, |
Line 1,028: |
Line 981: |
| band "now" at ~80kDa) quite well. </li> | | band "now" at ~80kDa) quite well. </li> |
| <li>Trafo of Lipocalin (klon2) and Digoxigenin (klon1) in XL1 | | <li>Trafo of Lipocalin (klon2) and Digoxigenin (klon1) in XL1 |
- | blue | + | blue </li> |
- | </li>
| + | <li>Ligation: Pmal+CAT with Temp.-shift (4-16°C) </li> |
- | <li>Ligation: Pmal+CAT with Temp.-shift (4-16°C) | + | |
- | </li>
| + | |
| <li>Trafo of the Ligation (above, 2µl and | | <li>Trafo of the Ligation (above, 2µl and |
| 5µl) and the old ligation (08.07., 4µl) </li> | | 5µl) and the old ligation (08.07., 4µl) </li> |
| <li>Trafo of His-Tag, Strep-Tag, Split-Linker and GGGs-Linker | | <li>Trafo of His-Tag, Strep-Tag, Split-Linker and GGGs-Linker |
- | in XL1 blue | + | in XL1 blue </li> |
- | </li>
| + | <li><b>Nanodrop data</b> </li> |
- | <li><b>Nanodrop data</b> | + | <li>Protein A-280 </li> |
- | </li>
| + | |
- | <li>Protein A-280 | + | |
- | </li>
| + | |
| </ul> | | </ul> |
| <table | | <table |
Line 1,049: |
Line 997: |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl1519412" style="height: 15pt; width: 84pt;" | | <td class="xl1519412" style="height: 15pt; width: 84pt;" |
- | height="20" width="112">Sample | + | height="20" width="112"><small>Sample |
- | ID</td> | + | ID</small></td> |
| <td class="xl1519412" style="width: 48pt;" | | <td class="xl1519412" style="width: 48pt;" |
- | width="64">User ID</td> | + | width="64"><small>User ID</small></td> |
| <td class="xl1519412" style="width: 53pt;" | | <td class="xl1519412" style="width: 53pt;" |
- | width="71">Date<span style=""> </span></td> | + | width="71"><small>Date<span style=""> </span></small></td> |
| <td class="xl1519412" style="width: 48pt;" | | <td class="xl1519412" style="width: 48pt;" |
- | width="64">Time<span style=""> </span></td> | + | width="64"><small>Time<span style=""> </span></small></td> |
| <td class="xl1519412" style="width: 48pt;" | | <td class="xl1519412" style="width: 48pt;" |
- | width="64">mg/ml<span style=""> </span></td> | + | width="64"><small>mg/ml<span style=""> </span></small></td> |
| <td class="xl1519412" style="width: 48pt;" | | <td class="xl1519412" style="width: 48pt;" |
- | width="64">260/280<span style=""> </span></td> | + | width="64"><small>260/280<span style=""> </span></small></td> |
| <td class="xl1519412" style="width: 66pt;" | | <td class="xl1519412" style="width: 66pt;" |
- | width="88">A280 10mm</td> | + | width="88"><small>A280 10mm</small></td> |
| <td class="xl1519412" style="width: 71pt;" | | <td class="xl1519412" style="width: 71pt;" |
- | width="94">E 1%</td> | + | width="94"><small>E 1%</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl1519412" style="height: 15pt;" | | <td class="xl1519412" style="height: 15pt;" |
- | height="20">BL21 Aa 1</td> | + | height="20"><small>BL21 Aa 1</small></td> |
- | <td class="xl1519412">FreiGEM</td> | + | <td class="xl1519412"><small>FreiGEM</small></td> |
- | <td class="xl6319412" align="right">09.07.2009</td> | + | <td class="xl6319412" align="right"><small>09.07.2009</small></td> |
- | <td class="xl6419412" align="right">11:45</td> | + | <td class="xl6419412" align="right"><small>11:45</small></td> |
- | <td class="xl1519412" align="right">0.11</td> | + | <td class="xl1519412" align="right"><small>0.11</small></td> |
- | <td class="xl1519412" align="right">1.48</td> | + | <td class="xl1519412" align="right"><small>1.48</small></td> |
- | <td class="xl1519412" align="right">0.114</td> | + | <td class="xl1519412" align="right"><small>0.114</small></td> |
- | <td class="xl1519412" align="right">10</td> | + | <td class="xl1519412" align="right"><small>10</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl1519412" style="height: 15pt;" | | <td class="xl1519412" style="height: 15pt;" |
- | height="20">BL21 Aa 1</td> | + | height="20"><small>BL21 Aa 1</small></td> |
- | <td class="xl1519412">FreiGEM</td> | + | <td class="xl1519412"><small>FreiGEM</small></td> |
- | <td class="xl6319412" align="right">09.07.2009</td> | + | <td class="xl6319412" align="right"><small>09.07.2009</small></td> |
- | <td class="xl6419412" align="right">11:46</td> | + | <td class="xl6419412" align="right"><small>11:46</small></td> |
- | <td class="xl1519412" align="right">0.11</td> | + | <td class="xl1519412" align="right"><small>0.11</small></td> |
- | <td class="xl1519412" align="right">1.44</td> | + | <td class="xl1519412" align="right"><small>1.44</small></td> |
- | <td class="xl1519412" align="right">0.113</td> | + | <td class="xl1519412" align="right"><small>0.113</small></td> |
- | <td class="xl1519412" align="right">10</td> | + | <td class="xl1519412" align="right"><small>10</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl1519412" style="height: 15pt;" | | <td class="xl1519412" style="height: 15pt;" |
- | height="20">BL21 Aa 2</td> | + | height="20"><small>BL21 Aa 2</small></td> |
- | <td class="xl1519412">FreiGEM</td> | + | <td class="xl1519412"><small>FreiGEM</small></td> |
- | <td class="xl6319412" align="right">09.07.2009</td> | + | <td class="xl6319412" align="right"><small>09.07.2009</small></td> |
- | <td class="xl6419412" align="right">11:47</td> | + | <td class="xl6419412" align="right"><small>11:47</small></td> |
- | <td class="xl1519412" align="right">0.39</td> | + | <td class="xl1519412" align="right"><small>0.39</small></td> |
- | <td class="xl1519412" align="right">1.58</td> | + | <td class="xl1519412" align="right"><small>1.58</small></td> |
- | <td class="xl1519412" align="right">0.389</td> | + | <td class="xl1519412" align="right"><small>0.389</small></td> |
- | <td class="xl1519412" align="right">10</td> | + | <td class="xl1519412" align="right"><small>10</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl1519412" style="height: 15pt;" | | <td class="xl1519412" style="height: 15pt;" |
- | height="20">BL21 Aa 2</td> | + | height="20"><small>BL21 Aa 2</small></td> |
- | <td class="xl1519412">FreiGEM</td> | + | <td class="xl1519412"><small>FreiGEM</small></td> |
- | <td class="xl6319412" align="right">09.07.2009</td> | + | <td class="xl6319412" align="right"><small>09.07.2009</small></td> |
- | <td class="xl6419412" align="right">11:47</td> | + | <td class="xl6419412" align="right"><small>11:47</small></td> |
- | <td class="xl1519412" align="right">0.2</td> | + | <td class="xl1519412" align="right"><small>0.2</small></td> |
- | <td class="xl1519412" align="right">1.65</td> | + | <td class="xl1519412" align="right"><small>1.65</small></td> |
- | <td class="xl1519412" align="right">0.204</td> | + | <td class="xl1519412" align="right"><small>0.204</small></td> |
- | <td class="xl1519412" align="right">10</td> | + | <td class="xl1519412" align="right"><small>10</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl1519412" style="height: 15pt;" | | <td class="xl1519412" style="height: 15pt;" |
- | height="20">BL21 Aa 2</td> | + | height="20"><small>BL21 Aa 2</small></td> |
- | <td class="xl1519412">FreiGEM</td> | + | <td class="xl1519412"><small>FreiGEM</small></td> |
- | <td class="xl6319412" align="right">09.07.2009</td> | + | <td class="xl6319412" align="right"><small>09.07.2009</small></td> |
- | <td class="xl6419412" align="right">11:48</td> | + | <td class="xl6419412" align="right"><small>11:48</small></td> |
- | <td class="xl1519412" align="right">0.22</td> | + | <td class="xl1519412" align="right"><small>0.22</small></td> |
- | <td class="xl1519412" align="right">1.79</td> | + | <td class="xl1519412" align="right"><small>1.79</small></td> |
- | <td class="xl1519412" align="right">0.223</td> | + | <td class="xl1519412" align="right"><small>0.223</small></td> |
- | <td class="xl1519412" align="right">10</td> | + | <td class="xl1519412" align="right"><small>10</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl1519412" style="height: 15pt;" | | <td class="xl1519412" style="height: 15pt;" |
- | height="20">BL21 Aa 3</td> | + | height="20"><small>BL21 Aa 3</small></td> |
- | <td class="xl1519412">FreiGEM</td> | + | <td class="xl1519412"><small>FreiGEM</small></td> |
- | <td class="xl6319412" align="right">09.07.2009</td> | + | <td class="xl6319412" align="right"><small>09.07.2009</small></td> |
- | <td class="xl6419412" align="right">11:49</td> | + | <td class="xl6419412" align="right"><small>11:49</small></td> |
- | <td class="xl1519412" align="right">0.42</td> | + | <td class="xl1519412" align="right"><small>0.42</small></td> |
- | <td class="xl1519412" align="right">1.33</td> | + | <td class="xl1519412" align="right"><small>1.33</small></td> |
- | <td class="xl1519412" align="right">0.417</td> | + | <td class="xl1519412" align="right"><small>0.417</small></td> |
- | <td class="xl1519412" align="right">10</td> | + | <td class="xl1519412" align="right"><small>10</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl1519412" style="height: 15pt;" | | <td class="xl1519412" style="height: 15pt;" |
- | height="20">BL21 Aa 3</td> | + | height="20"><small>BL21 Aa 3</small></td> |
- | <td class="xl1519412">FreiGEM</td> | + | <td class="xl1519412"><small>FreiGEM</small></td> |
- | <td class="xl6319412" align="right">09.07.2009</td> | + | <td class="xl6319412" align="right"><small>09.07.2009</small></td> |
- | <td class="xl6419412" align="right">11:50</td> | + | <td class="xl6419412" align="right"><small>11:50</small></td> |
- | <td class="xl1519412" align="right">0.43</td> | + | <td class="xl1519412" align="right"><small>0.43</small></td> |
- | <td class="xl1519412" align="right">1.52</td> | + | <td class="xl1519412" align="right"><small>1.52</small></td> |
- | <td class="xl1519412" align="right">0.429</td> | + | <td class="xl1519412" align="right"><small>0.429</small></td> |
- | <td class="xl1519412" align="right">10</td> | + | <td class="xl1519412" align="right"><small>10</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl1519412" style="height: 15pt;" | | <td class="xl1519412" style="height: 15pt;" |
- | height="20">BL21 Aa 4</td> | + | height="20"><small>BL21 Aa 4</small></td> |
- | <td class="xl1519412">FreiGEM</td> | + | <td class="xl1519412"><small>FreiGEM</small></td> |
- | <td class="xl6319412" align="right">09.07.2009</td> | + | <td class="xl6319412" align="right"><small>09.07.2009</small></td> |
- | <td class="xl6419412" align="right">11:50</td> | + | <td class="xl6419412" align="right"><small>11:50</small></td> |
- | <td class="xl1519412" align="right">0.18</td> | + | <td class="xl1519412" align="right"><small>0.18</small></td> |
- | <td class="xl1519412" align="right">1.53</td> | + | <td class="xl1519412" align="right"><small>1.53</small></td> |
- | <td class="xl1519412" align="right">0.183</td> | + | <td class="xl1519412" align="right"><small>0.183</small></td> |
- | <td class="xl1519412" align="right">10</td> | + | <td class="xl1519412" align="right"><small>10</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl1519412" style="height: 15pt;" | | <td class="xl1519412" style="height: 15pt;" |
- | height="20">BL21 Aa 4</td> | + | height="20"><small>BL21 Aa 4</small></td> |
- | <td class="xl1519412">FreiGEM</td> | + | <td class="xl1519412"><small>FreiGEM</small></td> |
- | <td class="xl6319412" align="right">09.07.2009</td> | + | <td class="xl6319412" align="right"><small>09.07.2009</small></td> |
- | <td class="xl6419412" align="right">11:51</td> | + | <td class="xl6419412" align="right"><small>11:51</small></td> |
- | <td class="xl1519412" align="right">0.55</td> | + | <td class="xl1519412" align="right"><small>0.55</small></td> |
- | <td class="xl1519412" align="right">1.18</td> | + | <td class="xl1519412" align="right"><small>1.18</small></td> |
- | <td class="xl1519412" align="right">0.552</td> | + | <td class="xl1519412" align="right"><small>0.552</small></td> |
- | <td class="xl1519412" align="right">10</td> | + | <td class="xl1519412" align="right"><small>10</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl1519412" style="height: 15pt;" | | <td class="xl1519412" style="height: 15pt;" |
- | height="20">BL21 Aa 4</td> | + | height="20"><small>BL21 Aa 4</small></td> |
- | <td class="xl1519412">FreiGEM</td> | + | <td class="xl1519412"><small>FreiGEM</small></td> |
- | <td class="xl6319412" align="right">09.07.2009</td> | + | <td class="xl6319412" align="right"><small>09.07.2009</small></td> |
- | <td class="xl6419412" align="right">11:52</td> | + | <td class="xl6419412" align="right"><small>11:52</small></td> |
- | <td class="xl1519412" align="right">0.19</td> | + | <td class="xl1519412" align="right"><small>0.19</small></td> |
- | <td class="xl1519412" align="right">0.87</td> | + | <td class="xl1519412" align="right"><small>0.87</small></td> |
- | <td class="xl1519412" align="right">0.187</td> | + | <td class="xl1519412" align="right"><small>0.187</small></td> |
- | <td class="xl1519412" align="right">10</td> | + | <td class="xl1519412" align="right"><small>10</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl1519412" style="height: 15pt;" | | <td class="xl1519412" style="height: 15pt;" |
- | height="20">BL21 Aa 5</td> | + | height="20"><small>BL21 Aa 5</small></td> |
- | <td class="xl1519412">FreiGEM</td> | + | <td class="xl1519412"><small>FreiGEM</small></td> |
- | <td class="xl6319412" align="right">09.07.2009</td> | + | <td class="xl6319412" align="right"><small>09.07.2009</small></td> |
- | <td class="xl6419412" align="right">11:53</td> | + | <td class="xl6419412" align="right"><small>11:53</small></td> |
- | <td class="xl1519412" align="right">0.28</td> | + | <td class="xl1519412" align="right"><small>0.28</small></td> |
- | <td class="xl1519412" align="right">0.78</td> | + | <td class="xl1519412" align="right"><small>0.78</small></td> |
- | <td class="xl1519412" align="right">0.282</td> | + | <td class="xl1519412" align="right"><small>0.282</small></td> |
- | <td class="xl1519412" align="right">10</td> | + | <td class="xl1519412" align="right"><small>10</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl1519412" style="height: 15pt;" | | <td class="xl1519412" style="height: 15pt;" |
- | height="20">BL21 Aa 5</td> | + | height="20"><small>BL21 Aa 5</small></td> |
- | <td class="xl1519412">FreiGEM</td> | + | <td class="xl1519412"><small>FreiGEM</small></td> |
- | <td class="xl6319412" align="right">09.07.2009</td> | + | <td class="xl6319412" align="right"><small>09.07.2009</small></td> |
- | <td class="xl6419412" align="right">11:54</td> | + | <td class="xl6419412" align="right"><small>11:54</small></td> |
- | <td class="xl1519412" align="right">0.27</td> | + | <td class="xl1519412" align="right"><small>0.27</small></td> |
- | <td class="xl1519412" align="right">0.81</td> | + | <td class="xl1519412" align="right"><small>0.81</small></td> |
- | <td class="xl1519412" align="right">0.269</td> | + | <td class="xl1519412" align="right"><small>0.269</small></td> |
- | <td class="xl1519412" align="right">10</td> | + | <td class="xl1519412" align="right"><small>10</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl1519412" style="height: 15pt;" | | <td class="xl1519412" style="height: 15pt;" |
- | height="20">BL21 Aa 6</td> | + | height="20"><small>BL21 Aa 6</small></td> |
- | <td class="xl1519412">FreiGEM</td> | + | <td class="xl1519412"><small>FreiGEM</small></td> |
- | <td class="xl6319412" align="right">09.07.2009</td> | + | <td class="xl6319412" align="right"><small>09.07.2009</small></td> |
- | <td class="xl6419412" align="right">11:54</td> | + | <td class="xl6419412" align="right"><small>11:54</small></td> |
- | <td class="xl1519412" align="right">0.22</td> | + | <td class="xl1519412" align="right"><small>0.22</small></td> |
- | <td class="xl1519412" align="right">0.79</td> | + | <td class="xl1519412" align="right"><small>0.79</small></td> |
- | <td class="xl1519412" align="right">0.222</td> | + | <td class="xl1519412" align="right"><small>0.222</small></td> |
- | <td class="xl1519412" align="right">10</td> | + | <td class="xl1519412" align="right"><small>10</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl1519412" style="height: 15pt;" | | <td class="xl1519412" style="height: 15pt;" |
- | height="20">BL21 Aa 6</td> | + | height="20"><small>BL21 Aa 6</small></td> |
- | <td class="xl1519412">FreiGEM</td> | + | <td class="xl1519412"><small>FreiGEM</small></td> |
- | <td class="xl6319412" align="right">09.07.2009</td> | + | <td class="xl6319412" align="right"><small>09.07.2009</small></td> |
- | <td class="xl6419412" align="right">11:55</td> | + | <td class="xl6419412" align="right"><small>11:55</small></td> |
- | <td class="xl1519412" align="right">0.23</td> | + | <td class="xl1519412" align="right"><small>0.23</small></td> |
- | <td class="xl1519412" align="right">0.73</td> | + | <td class="xl1519412" align="right"><small>0.73</small></td> |
- | <td class="xl1519412" align="right">0.231</td> | + | <td class="xl1519412" align="right"><small>0.231</small></td> |
- | <td class="xl1519412" align="right">10</td> | + | <td class="xl1519412" align="right"><small>10</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl1519412" style="height: 15pt;" | | <td class="xl1519412" style="height: 15pt;" |
- | height="20">BL21 Aa 7</td> | + | height="20"><small>BL21 Aa 7</small></td> |
- | <td class="xl1519412">FreiGEM</td> | + | <td class="xl1519412"><small>FreiGEM</small></td> |
- | <td class="xl6319412" align="right">09.07.2009</td> | + | <td class="xl6319412" align="right"><small>09.07.2009</small></td> |
- | <td class="xl6419412" align="right">11:56</td> | + | <td class="xl6419412" align="right"><small>11:56</small></td> |
- | <td class="xl1519412" align="right">0.21</td> | + | <td class="xl1519412" align="right"><small>0.21</small></td> |
- | <td class="xl1519412" align="right">0.76</td> | + | <td class="xl1519412" align="right"><small>0.76</small></td> |
- | <td class="xl1519412" align="right">0.209</td> | + | <td class="xl1519412" align="right"><small>0.209</small></td> |
- | <td class="xl1519412" align="right">10</td> | + | <td class="xl1519412" align="right"><small>10</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl1519412" style="height: 15pt;" | | <td class="xl1519412" style="height: 15pt;" |
- | height="20">BL21 Aa 7</td> | + | height="20"><small>BL21 Aa 7</small></td> |
- | <td class="xl1519412">FreiGEM</td> | + | <td class="xl1519412"><small>FreiGEM</small></td> |
- | <td class="xl6319412" align="right">09.07.2009</td> | + | <td class="xl6319412" align="right"><small>09.07.2009</small></td> |
- | <td class="xl6419412" align="right">11:56</td> | + | <td class="xl6419412" align="right"><small>11:56</small></td> |
- | <td class="xl1519412" align="right">0.2</td> | + | <td class="xl1519412" align="right"><small>0.2</small></td> |
- | <td class="xl1519412" align="right">0.75</td> | + | <td class="xl1519412" align="right"><small>0.75</small></td> |
- | <td class="xl1519412" align="right">0.204</td> | + | <td class="xl1519412" align="right"><small>0.204</small></td> |
- | <td class="xl1519412" align="right">10</td> | + | <td class="xl1519412" align="right"><small>10</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl1519412" style="height: 15pt;" | | <td class="xl1519412" style="height: 15pt;" |
- | height="20">BL21 Aa 8</td> | + | height="20"><small>BL21 Aa 8</small></td> |
- | <td class="xl1519412">FreiGEM</td> | + | <td class="xl1519412"><small>FreiGEM</small></td> |
- | <td class="xl6319412" align="right">09.07.2009</td> | + | <td class="xl6319412" align="right"><small>09.07.2009</small></td> |
- | <td class="xl6419412" align="right">11:57</td> | + | <td class="xl6419412" align="right"><small>11:57</small></td> |
- | <td class="xl1519412" align="right">0.22</td> | + | <td class="xl1519412" align="right"><small>0.22</small></td> |
- | <td class="xl1519412" align="right">0.75</td> | + | <td class="xl1519412" align="right"><small>0.75</small></td> |
- | <td class="xl1519412" align="right">0.222</td> | + | <td class="xl1519412" align="right"><small>0.222</small></td> |
- | <td class="xl1519412" align="right">10</td> | + | <td class="xl1519412" align="right"><small>10</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl1519412" style="height: 15pt;" | | <td class="xl1519412" style="height: 15pt;" |
- | height="20">BL21 Aa 8</td> | + | height="20"><small>BL21 Aa 8</small></td> |
- | <td class="xl1519412">FreiGEM</td> | + | <td class="xl1519412"><small>FreiGEM</small></td> |
- | <td class="xl6319412" align="right">09.07.2009</td> | + | <td class="xl6319412" align="right"><small>09.07.2009</small></td> |
- | <td class="xl6419412" align="right">11:57</td> | + | <td class="xl6419412" align="right"><small>11:57</small></td> |
- | <td class="xl1519412" align="right">0.22</td> | + | <td class="xl1519412" align="right"><small>0.22</small></td> |
- | <td class="xl1519412" align="right">0.73</td> | + | <td class="xl1519412" align="right"><small>0.73</small></td> |
- | <td class="xl1519412" align="right">0.22</td> | + | <td class="xl1519412" align="right"><small>0.22</small></td> |
- | <td class="xl1519412" align="right">10</td> | + | <td class="xl1519412" align="right"><small>10</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl1519412" style="height: 15pt;" | | <td class="xl1519412" style="height: 15pt;" |
- | height="20">BL21 Aa 9</td> | + | height="20"><small>BL21 Aa 9</small></td> |
- | <td class="xl1519412">FreiGEM</td> | + | <td class="xl1519412"><small>FreiGEM</small></td> |
- | <td class="xl6319412" align="right">09.07.2009</td> | + | <td class="xl6319412" align="right"><small>09.07.2009</small></td> |
- | <td class="xl6419412" align="right">11:58</td> | + | <td class="xl6419412" align="right"><small>11:58</small></td> |
- | <td class="xl1519412" align="right">0.2</td> | + | <td class="xl1519412" align="right"><small>0.2</small></td> |
- | <td class="xl1519412" align="right">0.72</td> | + | <td class="xl1519412" align="right"><small>0.72</small></td> |
- | <td class="xl1519412" align="right">0.197</td> | + | <td class="xl1519412" align="right"><small>0.197</small></td> |
- | <td class="xl1519412" align="right">10</td> | + | <td class="xl1519412" align="right"><small>10</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl1519412" style="height: 15pt;" | | <td class="xl1519412" style="height: 15pt;" |
- | height="20">BL21 Aa 9</td> | + | height="20"><small>BL21 Aa 9</small></td> |
- | <td class="xl1519412">FreiGEM</td> | + | <td class="xl1519412"><small>FreiGEM</small></td> |
- | <td class="xl6319412" align="right">09.07.2009</td> | + | <td class="xl6319412" align="right"><small>09.07.2009</small></td> |
- | <td class="xl6419412" align="right">11:58</td> | + | <td class="xl6419412" align="right"><small>11:58</small></td> |
- | <td class="xl1519412" align="right">0.21</td> | + | <td class="xl1519412" align="right"><small>0.21</small></td> |
- | <td class="xl1519412" align="right">0.74</td> | + | <td class="xl1519412" align="right"><small>0.74</small></td> |
- | <td class="xl1519412" align="right">0.206</td> | + | <td class="xl1519412" align="right"><small>0.206</small></td> |
- | <td class="xl1519412" align="right">10</td> | + | <td class="xl1519412" align="right"><small>10</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl6519412" style="height: 15pt;" | | <td class="xl6519412" style="height: 15pt;" |
- | height="20">BL21 Aa 10</td> | + | height="20"><small>BL21 Aa 10</small></td> |
- | <td class="xl1519412">FreiGEM</td> | + | <td class="xl1519412"><small>FreiGEM</small></td> |
- | <td class="xl6319412" align="right">09.07.2009</td> | + | <td class="xl6319412" align="right"><small>09.07.2009</small></td> |
- | <td class="xl6419412" align="right">11:59</td> | + | <td class="xl6419412" align="right"><small>11:59</small></td> |
- | <td class="xl1519412" align="right">0.26</td> | + | <td class="xl1519412" align="right"><small>0.26</small></td> |
- | <td class="xl1519412" align="right">1.02</td> | + | <td class="xl1519412" align="right"><small>1.02</small></td> |
- | <td class="xl1519412" align="right">0.264</td> | + | <td class="xl1519412" align="right"><small>0.264</small></td> |
- | <td class="xl1519412" align="right">10</td> | + | <td class="xl1519412" align="right"><small>10</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl6519412" style="height: 15pt;" | | <td class="xl6519412" style="height: 15pt;" |
- | height="20">BL21 Aa 10</td> | + | height="20"><small>BL21 Aa 10</small></td> |
- | <td class="xl1519412">FreiGEM</td> | + | <td class="xl1519412"><small>FreiGEM</small></td> |
- | <td class="xl6319412" align="right">09.07.2009</td> | + | <td class="xl6319412" align="right"><small>09.07.2009</small></td> |
- | <td class="xl6419412" align="right">11:59</td> | + | <td class="xl6419412" align="right"><small>11:59</small></td> |
- | <td class="xl1519412" align="right">0.21</td> | + | <td class="xl1519412" align="right"><small>0.21</small></td> |
- | <td class="xl1519412" align="right">0.73</td> | + | <td class="xl1519412" align="right"><small>0.73</small></td> |
- | <td class="xl1519412" align="right">0.206</td> | + | <td class="xl1519412" align="right"><small>0.206</small></td> |
- | <td class="xl1519412" align="right">10</td> | + | <td class="xl1519412" align="right"><small>10</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl6519412" style="height: 15pt;" | | <td class="xl6519412" style="height: 15pt;" |
- | height="20">BL21 Aa 11</td> | + | height="20"><small>BL21 Aa 11</small></td> |
- | <td class="xl1519412">FreiGEM</td> | + | <td class="xl1519412"><small>FreiGEM</small></td> |
- | <td class="xl6319412" align="right">09.07.2009</td> | + | <td class="xl6319412" align="right"><small>09.07.2009</small></td> |
- | <td class="xl6419412" align="right">12:00</td> | + | <td class="xl6419412" align="right"><small>12:00</small></td> |
- | <td class="xl1519412" align="right">0.2</td> | + | <td class="xl1519412" align="right"><small>0.2</small></td> |
- | <td class="xl1519412" align="right">0.73</td> | + | <td class="xl1519412" align="right"><small>0.73</small></td> |
- | <td class="xl1519412" align="right">0.2</td> | + | <td class="xl1519412" align="right"><small>0.2</small></td> |
- | <td class="xl1519412" align="right">10</td> | + | <td class="xl1519412" align="right"><small>10</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl6519412" style="height: 15pt;" | | <td class="xl6519412" style="height: 15pt;" |
- | height="20">BL21 Aa 11</td> | + | height="20"><small>BL21 Aa 11</small></td> |
- | <td class="xl1519412">FreiGEM</td> | + | <td class="xl1519412"><small>FreiGEM</small></td> |
- | <td class="xl6319412" align="right">09.07.2009</td> | + | <td class="xl6319412" align="right"><small>09.07.2009</small></td> |
- | <td class="xl6419412" align="right">12:00</td> | + | <td class="xl6419412" align="right"><small>12:00</small></td> |
- | <td class="xl1519412" align="right">0.22</td> | + | <td class="xl1519412" align="right"><small>0.22</small></td> |
- | <td class="xl1519412" align="right">0.73</td> | + | <td class="xl1519412" align="right"><small>0.73</small></td> |
- | <td class="xl1519412" align="right">0.217</td> | + | <td class="xl1519412" align="right"><small>0.217</small></td> |
- | <td class="xl1519412" align="right">10</td> | + | <td class="xl1519412" align="right"><small>10</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl6519412" style="height: 15pt;" | | <td class="xl6519412" style="height: 15pt;" |
- | height="20">T7 Aa</td> | + | height="20"><small>T7 Aa</small></td> |
- | <td class="xl1519412">FreiGEM</td> | + | <td class="xl1519412"><small>FreiGEM</small></td> |
- | <td class="xl6319412" align="right">09.07.2009</td> | + | <td class="xl6319412" align="right"><small>09.07.2009</small></td> |
- | <td class="xl6419412" align="right">12:03</td> | + | <td class="xl6419412" align="right"><small>12:03</small></td> |
- | <td class="xl1519412" align="right">5.71</td> | + | <td class="xl1519412" align="right"><small>5.71</small></td> |
- | <td class="xl1519412" align="right">2</td> | + | <td class="xl1519412" align="right"><small>2</small></td> |
- | <td class="xl1519412" align="right">5.711</td> | + | <td class="xl1519412" align="right"><small>5.711</small></td> |
- | <td class="xl1519412" align="right">10</td> | + | <td class="xl1519412" align="right"><small>10</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl6519412" style="height: 15pt;" | | <td class="xl6519412" style="height: 15pt;" |
- | height="20">T7 Aa 1</td> | + | height="20"><small>T7 Aa 1</small></td> |
- | <td class="xl1519412">FreiGEM</td> | + | <td class="xl1519412"><small>FreiGEM</small></td> |
- | <td class="xl6319412" align="right">09.07.2009</td> | + | <td class="xl6319412" align="right"><small>09.07.2009</small></td> |
- | <td class="xl6419412" align="right">12:03</td> | + | <td class="xl6419412" align="right"><small>12:03</small></td> |
- | <td class="xl1519412" align="right">0.4</td> | + | <td class="xl1519412" align="right"><small>0.4</small></td> |
- | <td class="xl1519412" align="right">1.38</td> | + | <td class="xl1519412" align="right"><small>1.38</small></td> |
- | <td class="xl1519412" align="right">0.395</td> | + | <td class="xl1519412" align="right"><small>0.395</small></td> |
- | <td class="xl1519412" align="right">10</td> | + | <td class="xl1519412" align="right"><small>10</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl6519412" style="height: 15pt;" | | <td class="xl6519412" style="height: 15pt;" |
- | height="20">T7 Aa 1</td> | + | height="20"><small>T7 Aa 1</small></td> |
- | <td class="xl1519412">FreiGEM</td> | + | <td class="xl1519412"><small>FreiGEM</small></td> |
- | <td class="xl6319412" align="right">09.07.2009</td> | + | <td class="xl6319412" align="right"><small>09.07.2009</small></td> |
- | <td class="xl6419412" align="right">12:04</td> | + | <td class="xl6419412" align="right"><small>12:04</small></td> |
- | <td class="xl1519412" align="right">0.12</td> | + | <td class="xl1519412" align="right"><small>0.12</small></td> |
- | <td class="xl1519412" align="right">1.57</td> | + | <td class="xl1519412" align="right"><small>1.57</small></td> |
- | <td class="xl1519412" align="right">0.125</td> | + | <td class="xl1519412" align="right"><small>0.125</small></td> |
- | <td class="xl1519412" align="right">10</td> | + | <td class="xl1519412" align="right"><small>10</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl6519412" style="height: 15pt;" | | <td class="xl6519412" style="height: 15pt;" |
- | height="20">T7 Aa 1</td> | + | height="20"><small>T7 Aa 1</small></td> |
- | <td class="xl1519412">FreiGEM</td> | + | <td class="xl1519412"><small>FreiGEM</small></td> |
- | <td class="xl6319412" align="right">09.07.2009</td> | + | <td class="xl6319412" align="right"><small>09.07.2009</small></td> |
- | <td class="xl6419412" align="right">12:05</td> | + | <td class="xl6419412" align="right"><small>12:05</small></td> |
- | <td class="xl1519412" align="right">0.15</td> | + | <td class="xl1519412" align="right"><small>0.15</small></td> |
- | <td class="xl1519412" align="right">1.42</td> | + | <td class="xl1519412" align="right"><small>1.42</small></td> |
- | <td class="xl1519412" align="right">0.146</td> | + | <td class="xl1519412" align="right"><small>0.146</small></td> |
- | <td class="xl1519412" align="right">10</td> | + | <td class="xl1519412" align="right"><small>10</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl6519412" style="height: 15pt;" | | <td class="xl6519412" style="height: 15pt;" |
- | height="20">T7 Aa 2</td> | + | height="20"><small>T7 Aa 2</small></td> |
- | <td class="xl1519412">FreiGEM</td> | + | <td class="xl1519412"><small>FreiGEM</small></td> |
- | <td class="xl6319412" align="right">09.07.2009</td> | + | <td class="xl6319412" align="right"><small>09.07.2009</small></td> |
- | <td class="xl6419412" align="right">12:05</td> | + | <td class="xl6419412" align="right"><small>12:05</small></td> |
- | <td class="xl1519412" align="right">0.19</td> | + | <td class="xl1519412" align="right"><small>0.19</small></td> |
- | <td class="xl1519412" align="right">1.39</td> | + | <td class="xl1519412" align="right"><small>1.39</small></td> |
- | <td class="xl1519412" align="right">0.195</td> | + | <td class="xl1519412" align="right"><small>0.195</small></td> |
- | <td class="xl1519412" align="right">10</td> | + | <td class="xl1519412" align="right"><small>10</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl6519412" style="height: 15pt;" | | <td class="xl6519412" style="height: 15pt;" |
- | height="20">T7 Aa 2</td> | + | height="20"><small>T7 Aa 2</small></td> |
- | <td class="xl1519412">FreiGEM</td> | + | <td class="xl1519412"><small>FreiGEM</small></td> |
- | <td class="xl6319412" align="right">09.07.2009</td> | + | <td class="xl6319412" align="right"><small>09.07.2009</small></td> |
- | <td class="xl6419412" align="right">12:06</td> | + | <td class="xl6419412" align="right"><small>12:06</small></td> |
- | <td class="xl1519412" align="right">0.59</td> | + | <td class="xl1519412" align="right"><small>0.59</small></td> |
- | <td class="xl1519412" align="right">1.37</td> | + | <td class="xl1519412" align="right"><small>1.37</small></td> |
- | <td class="xl1519412" align="right">0.589</td> | + | <td class="xl1519412" align="right"><small>0.589</small></td> |
- | <td class="xl1519412" align="right">10</td> | + | <td class="xl1519412" align="right"><small>10</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl6519412" style="height: 15pt;" | | <td class="xl6519412" style="height: 15pt;" |
- | height="20">T7 Aa 2</td> | + | height="20"><small>T7 Aa 2</small></td> |
- | <td class="xl1519412">FreiGEM</td> | + | <td class="xl1519412"><small>FreiGEM</small></td> |
- | <td class="xl6319412" align="right">09.07.2009</td> | + | <td class="xl6319412" align="right"><small>09.07.2009</small></td> |
- | <td class="xl6419412" align="right">12:07</td> | + | <td class="xl6419412" align="right"><small>12:07</small></td> |
- | <td class="xl1519412" align="right">0.23</td> | + | <td class="xl1519412" align="right"><small>0.23</small></td> |
- | <td class="xl1519412" align="right">1.5</td> | + | <td class="xl1519412" align="right"><small>1.5</small></td> |
- | <td class="xl1519412" align="right">0.228</td> | + | <td class="xl1519412" align="right"><small>0.228</small></td> |
- | <td class="xl1519412" align="right">10</td> | + | <td class="xl1519412" align="right"><small>10</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl6519412" style="height: 15pt;" | | <td class="xl6519412" style="height: 15pt;" |
- | height="20">T7 Aa 3</td> | + | height="20"><small>T7 Aa 3</small></td> |
- | <td class="xl1519412">FreiGEM</td> | + | <td class="xl1519412"><small>FreiGEM</small></td> |
- | <td class="xl6319412" align="right">09.07.2009</td> | + | <td class="xl6319412" align="right"><small>09.07.2009</small></td> |
- | <td class="xl6419412" align="right">12:07</td> | + | <td class="xl6419412" align="right"><small>12:07</small></td> |
- | <td class="xl1519412" align="right">1.1</td> | + | <td class="xl1519412" align="right"><small>1.1</small></td> |
- | <td class="xl1519412" align="right">0.76</td> | + | <td class="xl1519412" align="right"><small>0.76</small></td> |
- | <td class="xl1519412" align="right">1.099</td> | + | <td class="xl1519412" align="right"><small>1.099</small></td> |
- | <td class="xl1519412" align="right">10</td> | + | <td class="xl1519412" align="right"><small>10</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl6519412" style="height: 15pt;" | | <td class="xl6519412" style="height: 15pt;" |
- | height="20">T7 Aa 3</td> | + | height="20"><small>T7 Aa 3</small></td> |
- | <td class="xl1519412">FreiGEM</td> | + | <td class="xl1519412"><small>FreiGEM</small></td> |
- | <td class="xl6319412" align="right">09.07.2009</td> | + | <td class="xl6319412" align="right"><small>09.07.2009</small></td> |
- | <td class="xl6419412" align="right">12:08</td> | + | <td class="xl6419412" align="right"><small>12:08</small></td> |
- | <td class="xl1519412" align="right">0.46</td> | + | <td class="xl1519412" align="right"><small>0.46</small></td> |
- | <td class="xl1519412" align="right">0.97</td> | + | <td class="xl1519412" align="right"><small>0.97</small></td> |
- | <td class="xl1519412" align="right">0.456</td> | + | <td class="xl1519412" align="right"><small>0.456</small></td> |
- | <td class="xl1519412" align="right">10</td> | + | <td class="xl1519412" align="right"><small>10</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl6519412" style="height: 15pt;" | | <td class="xl6519412" style="height: 15pt;" |
- | height="20">T7 Aa 3</td> | + | height="20"><small>T7 Aa 3</small></td> |
- | <td class="xl1519412">FreiGEM</td> | + | <td class="xl1519412"><small>FreiGEM</small></td> |
- | <td class="xl6319412" align="right">09.07.2009</td> | + | <td class="xl6319412" align="right"><small>09.07.2009</small></td> |
- | <td class="xl6419412" align="right">12:08</td> | + | <td class="xl6419412" align="right"><small>12:08</small></td> |
- | <td class="xl1519412" align="right">0.39</td> | + | <td class="xl1519412" align="right"><small>0.39</small></td> |
- | <td class="xl1519412" align="right">0.69</td> | + | <td class="xl1519412" align="right"><small>0.69</small></td> |
- | <td class="xl1519412" align="right">0.386</td> | + | <td class="xl1519412" align="right"><small>0.386</small></td> |
- | <td class="xl1519412" align="right">10</td> | + | <td class="xl1519412" align="right"><small>10</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl6519412" style="height: 15pt;" | | <td class="xl6519412" style="height: 15pt;" |
- | height="20">T7 Aa 4</td> | + | height="20"><small>T7 Aa 4</small></td> |
- | <td class="xl1519412">FreiGEM</td> | + | <td class="xl1519412"><small>FreiGEM</small></td> |
- | <td class="xl6319412" align="right">09.07.2009</td> | + | <td class="xl6319412" align="right"><small>09.07.2009</small></td> |
- | <td class="xl6419412" align="right">12:09</td> | + | <td class="xl6419412" align="right"><small>12:09</small></td> |
- | <td class="xl1519412" align="right">0.23</td> | + | <td class="xl1519412" align="right"><small>0.23</small></td> |
- | <td class="xl1519412" align="right">0.68</td> | + | <td class="xl1519412" align="right"><small>0.68</small></td> |
- | <td class="xl1519412" align="right">0.228</td> | + | <td class="xl1519412" align="right"><small>0.228</small></td> |
- | <td class="xl1519412" align="right">10</td> | + | <td class="xl1519412" align="right"><small>10</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl6519412" style="height: 15pt;" | | <td class="xl6519412" style="height: 15pt;" |
- | height="20">T7 Aa 4</td> | + | height="20"><small>T7 Aa 4</small></td> |
- | <td class="xl1519412">FreiGEM</td> | + | <td class="xl1519412"><small>FreiGEM</small></td> |
- | <td class="xl6319412" align="right">09.07.2009</td> | + | <td class="xl6319412" align="right"><small>09.07.2009</small></td> |
- | <td class="xl6419412" align="right">12:10</td> | + | <td class="xl6419412" align="right"><small>12:10</small></td> |
- | <td class="xl1519412" align="right">0.37</td> | + | <td class="xl1519412" align="right"><small>0.37</small></td> |
- | <td class="xl1519412" align="right">0.62</td> | + | <td class="xl1519412" align="right"><small>0.62</small></td> |
- | <td class="xl1519412" align="right">0.367</td> | + | <td class="xl1519412" align="right"><small>0.367</small></td> |
- | <td class="xl1519412" align="right">10</td> | + | <td class="xl1519412" align="right"><small>10</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl6519412" style="height: 15pt;" | | <td class="xl6519412" style="height: 15pt;" |
- | height="20">T7 Aa 5</td> | + | height="20"><small>T7 Aa 5</small></td> |
- | <td class="xl1519412">FreiGEM</td> | + | <td class="xl1519412"><small>FreiGEM</small></td> |
- | <td class="xl6319412" align="right">09.07.2009</td> | + | <td class="xl6319412" align="right"><small>09.07.2009</small></td> |
- | <td class="xl6419412" align="right">12:11</td> | + | <td class="xl6419412" align="right"><small>12:11</small></td> |
- | <td class="xl1519412" align="right">0.17</td> | + | <td class="xl1519412" align="right"><small>0.17</small></td> |
- | <td class="xl1519412" align="right">0.7</td> | + | <td class="xl1519412" align="right"><small>0.7</small></td> |
- | <td class="xl1519412" align="right">0.175</td> | + | <td class="xl1519412" align="right"><small>0.175</small></td> |
- | <td class="xl1519412" align="right">10</td> | + | <td class="xl1519412" align="right"><small>10</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl6519412" style="height: 15pt;" | | <td class="xl6519412" style="height: 15pt;" |
- | height="20">T7 Aa 5</td> | + | height="20"><small>T7 Aa 5</small></td> |
- | <td class="xl1519412">FreiGEM</td> | + | <td class="xl1519412"><small>FreiGEM</small></td> |
- | <td class="xl6319412" align="right">09.07.2009</td> | + | <td class="xl6319412" align="right"><small>09.07.2009</small></td> |
- | <td class="xl6419412" align="right">12:11</td> | + | <td class="xl6419412" align="right"><small>12:11</small></td> |
- | <td class="xl1519412" align="right">0.18</td> | + | <td class="xl1519412" align="right"><small>0.18</small></td> |
- | <td class="xl1519412" align="right">0.69</td> | + | <td class="xl1519412" align="right"><small>0.69</small></td> |
- | <td class="xl1519412" align="right">0.177</td> | + | <td class="xl1519412" align="right"><small>0.177</small></td> |
- | <td class="xl1519412" align="right">10</td> | + | <td class="xl1519412" align="right"><small>10</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl6519412" style="height: 15pt;" | | <td class="xl6519412" style="height: 15pt;" |
- | height="20">T7 Aa 6</td> | + | height="20"><small>T7 Aa 6</small></td> |
- | <td class="xl1519412">FreiGEM</td> | + | <td class="xl1519412"><small>FreiGEM</small></td> |
- | <td class="xl6319412" align="right">09.07.2009</td> | + | <td class="xl6319412" align="right"><small>09.07.2009</small></td> |
- | <td class="xl6419412" align="right">12:12</td> | + | <td class="xl6419412" align="right"><small>12:12</small></td> |
- | <td class="xl1519412" align="right">0.2</td> | + | <td class="xl1519412" align="right"><small>0.2</small></td> |
- | <td class="xl1519412" align="right">0.85</td> | + | <td class="xl1519412" align="right"><small>0.85</small></td> |
- | <td class="xl1519412" align="right">0.202</td> | + | <td class="xl1519412" align="right"><small>0.202</small></td> |
- | <td class="xl1519412" align="right">10</td> | + | <td class="xl1519412" align="right"><small>10</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl6519412" style="height: 15pt;" | | <td class="xl6519412" style="height: 15pt;" |
- | height="20">T7 Aa 6</td> | + | height="20"><small>T7 Aa 6</small></td> |
- | <td class="xl1519412">FreiGEM</td> | + | <td class="xl1519412"><small>FreiGEM</small></td> |
- | <td class="xl6319412" align="right">09.07.2009</td> | + | <td class="xl6319412" align="right"><small>09.07.2009</small></td> |
- | <td class="xl6419412" align="right">12:12</td> | + | <td class="xl6419412" align="right"><small>12:12</small></td> |
- | <td class="xl1519412" align="right">0.27</td> | + | <td class="xl1519412" align="right"><small>0.27</small></td> |
- | <td class="xl1519412" align="right">0.93</td> | + | <td class="xl1519412" align="right"><small>0.93</small></td> |
- | <td class="xl1519412" align="right">0.268</td> | + | <td class="xl1519412" align="right"><small>0.268</small></td> |
- | <td class="xl1519412" align="right">10</td> | + | <td class="xl1519412" align="right"><small>10</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl6519412" style="height: 15pt;" | | <td class="xl6519412" style="height: 15pt;" |
- | height="20">T7 Aa 6</td> | + | height="20"><small>T7 Aa 6</small></td> |
- | <td class="xl1519412">FreiGEM</td> | + | <td class="xl1519412"><small>FreiGEM</small></td> |
- | <td class="xl6319412" align="right">09.07.2009</td> | + | <td class="xl6319412" align="right"><small>09.07.2009</small></td> |
- | <td class="xl6419412" align="right">12:12</td> | + | <td class="xl6419412" align="right"><small>12:12</small></td> |
- | <td class="xl1519412" align="right">0.27</td> | + | <td class="xl1519412" align="right"><small>0.27</small></td> |
- | <td class="xl1519412" align="right">1.11</td> | + | <td class="xl1519412" align="right"><small>1.11</small></td> |
- | <td class="xl1519412" align="right">0.269</td> | + | <td class="xl1519412" align="right"><small>0.269</small></td> |
- | <td class="xl1519412" align="right">10</td> | + | <td class="xl1519412" align="right"><small>10</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl6519412" style="height: 15pt;" | | <td class="xl6519412" style="height: 15pt;" |
- | height="20">T7 Aa 7</td> | + | height="20"><small>T7 Aa 7</small></td> |
- | <td class="xl1519412">FreiGEM</td> | + | <td class="xl1519412"><small>FreiGEM</small></td> |
- | <td class="xl6319412" align="right">09.07.2009</td> | + | <td class="xl6319412" align="right"><small>09.07.2009</small></td> |
- | <td class="xl6419412" align="right">12:13</td> | + | <td class="xl6419412" align="right"><small>12:13</small></td> |
- | <td class="xl1519412" align="right">0.27</td> | + | <td class="xl1519412" align="right"><small>0.27</small></td> |
- | <td class="xl1519412" align="right">0.7</td> | + | <td class="xl1519412" align="right"><small>0.7</small></td> |
- | <td class="xl1519412" align="right">0.268</td> | + | <td class="xl1519412" align="right"><small>0.268</small></td> |
- | <td class="xl1519412" align="right">10</td> | + | <td class="xl1519412" align="right"><small>10</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl6519412" style="height: 15pt;" | | <td class="xl6519412" style="height: 15pt;" |
- | height="20">T7 Aa 7</td> | + | height="20"><small>T7 Aa 7</small></td> |
- | <td class="xl1519412">FreiGEM</td> | + | <td class="xl1519412"><small>FreiGEM</small></td> |
- | <td class="xl6319412" align="right">09.07.2009</td> | + | <td class="xl6319412" align="right"><small>09.07.2009</small></td> |
- | <td class="xl6419412" align="right">12:13</td> | + | <td class="xl6419412" align="right"><small>12:13</small></td> |
- | <td class="xl1519412" align="right">0.26</td> | + | <td class="xl1519412" align="right"><small>0.26</small></td> |
- | <td class="xl1519412" align="right">0.73</td> | + | <td class="xl1519412" align="right"><small>0.73</small></td> |
- | <td class="xl1519412" align="right">0.255</td> | + | <td class="xl1519412" align="right"><small>0.255</small></td> |
- | <td class="xl1519412" align="right">10</td> | + | <td class="xl1519412" align="right"><small>10</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl6519412" style="height: 15pt;" | | <td class="xl6519412" style="height: 15pt;" |
- | height="20">T7 Aa 8</td> | + | height="20"><small>T7 Aa 8</small></td> |
- | <td class="xl1519412">FreiGEM</td> | + | <td class="xl1519412"><small>FreiGEM</small></td> |
- | <td class="xl6319412" align="right">09.07.2009</td> | + | <td class="xl6319412" align="right"><small>09.07.2009</small></td> |
- | <td class="xl6419412" align="right">12:14</td> | + | <td class="xl6419412" align="right"><small>12:14</small></td> |
- | <td class="xl1519412" align="right">0.22</td> | + | <td class="xl1519412" align="right"><small>0.22</small></td> |
- | <td class="xl1519412" align="right">0.76</td> | + | <td class="xl1519412" align="right"><small>0.76</small></td> |
- | <td class="xl1519412" align="right">0.219</td> | + | <td class="xl1519412" align="right"><small>0.219</small></td> |
- | <td class="xl1519412" align="right">10</td> | + | <td class="xl1519412" align="right"><small>10</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl6519412" style="height: 15pt;" | | <td class="xl6519412" style="height: 15pt;" |
- | height="20">T7 Aa 8</td> | + | height="20"><small>T7 Aa 8</small></td> |
- | <td class="xl1519412">FreiGEM</td> | + | <td class="xl1519412"><small>FreiGEM</small></td> |
- | <td class="xl6319412" align="right">09.07.2009</td> | + | <td class="xl6319412" align="right"><small>09.07.2009</small></td> |
- | <td class="xl6419412" align="right">12:14</td> | + | <td class="xl6419412" align="right"><small>12:14</small></td> |
- | <td class="xl1519412" align="right">0.18</td> | + | <td class="xl1519412" align="right"><small>0.18</small></td> |
- | <td class="xl1519412" align="right">0.79</td> | + | <td class="xl1519412" align="right"><small>0.79</small></td> |
- | <td class="xl1519412" align="right">0.176</td> | + | <td class="xl1519412" align="right"><small>0.176</small></td> |
- | <td class="xl1519412" align="right">10</td> | + | <td class="xl1519412" align="right"><small>10</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl6519412" style="height: 15pt;" | | <td class="xl6519412" style="height: 15pt;" |
- | height="20">T7 Aa 8</td> | + | height="20"><small>T7 Aa 8</small></td> |
- | <td class="xl1519412">FreiGEM</td> | + | <td class="xl1519412"><small>FreiGEM</small></td> |
- | <td class="xl6319412" align="right">09.07.2009</td> | + | <td class="xl6319412" align="right"><small>09.07.2009</small></td> |
- | <td class="xl6419412" align="right">12:14</td> | + | <td class="xl6419412" align="right"><small>12:14</small></td> |
- | <td class="xl1519412" align="right">0.2</td> | + | <td class="xl1519412" align="right"><small>0.2</small></td> |
- | <td class="xl1519412" align="right">0.79</td> | + | <td class="xl1519412" align="right"><small>0.79</small></td> |
- | <td class="xl1519412" align="right">0.196</td> | + | <td class="xl1519412" align="right"><small>0.196</small></td> |
- | <td class="xl1519412" align="right">10</td> | + | <td class="xl1519412" align="right"><small>10</small></td> |
| </tr> | | </tr> |
| <tr style="display: none;" height="0"> | | <tr style="display: none;" height="0"> |
Line 1,592: |
Line 1,540: |
| <li>Trafo of His-Tag, Strep-Tag, Split-Linker showed good | | <li>Trafo of His-Tag, Strep-Tag, Split-Linker showed good |
| results; GGGs-Linker showed no grown colonies -> inoculation on | | results; GGGs-Linker showed no grown colonies -> inoculation on |
- | monday | + | monday </li> |
- | </li>
| + | |
| <li>SDS-Gel with samples of His-Tag purification via Ni-NTA | | <li>SDS-Gel with samples of His-Tag purification via Ni-NTA |
- | column and quick staining with comassie | + | column and quick staining with comassie </li> |
- | </li>
| + | |
| </ul> | | </ul> |
| <h3>13.07.09, 09:00-15:00 Uhr Laura, Christoph, Hannes, Caro, | | <h3>13.07.09, 09:00-15:00 Uhr Laura, Christoph, Hannes, Caro, |
Line 1,602: |
Line 1,548: |
| <br /> | | <br /> |
| <ul> | | <ul> |
- | <li>inoculation of His, Strep, Split-Linker in pMa (Amp) | + | <li>inoculation of His, Strep, Split-Linker in pMa (Amp) </li> |
- | </li>
| + | <li>inoculation of the ligation-trafo from last week (CAT) </li> |
- | <li>inoculation of the ligation-trafo from last week (CAT) | + | <li>we did 4 SDS-gels (stored at 4°C) </li> |
- | </li>
| + | |
- | <li>we did 4 SDS-gels (stored at 4°C) | + | |
- | </li>
| + | |
| </ul> | | </ul> |
| <ul> | | <ul> |
- | <li>we prepared 1l of 2YT-medium | + | <li>we prepared 1l of 2YT-medium </li> |
- | </li>
| + | |
| </ul> | | </ul> |
| <h3>14.07.09, 14:00-21:00 Uhr Gerrit, Julia, Dieter, Max, Imi, | | <h3>14.07.09, 14:00-21:00 Uhr Gerrit, Julia, Dieter, Max, Imi, |
Line 1,619: |
Line 1,561: |
| <li>Plasmid-Preparation of His-Tag, Strep-Tag, Split-Linker, | | <li>Plasmid-Preparation of His-Tag, Strep-Tag, Split-Linker, |
| Ligation 9.7. (neu2), Ligation 9.7. (alt), Ligation 8.7. (100 | | Ligation 9.7. (neu2), Ligation 9.7. (alt), Ligation 8.7. (100 |
- | µl and rest) | + | µl and rest) </li> |
- | </li>
| + | |
| <li>Digestion of FOKI, FOKA with XbaI and PSTI and SPLIT- | | <li>Digestion of FOKI, FOKA with XbaI and PSTI and SPLIT- |
- | Linker with SpeI, PstI. | + | Linker with SpeI, PstI. </li> |
- | </li>
| + | <li>Applied on 1% Agarose- Gel and sliced out </li> |
- | <li>Applied on 1% Agarose- Gel and sliced out | + | |
- | </li>
| + | |
| <li>DNA Gel Extraction with QuiaGen Kit according to the | | <li>DNA Gel Extraction with QuiaGen Kit according to the |
- | protocol | + | protocol </li> |
- | </li>
| + | |
| <li>Result: Didnt work (FOKI= 6,28 ng/µl; SPLIT=11,43 | | <li>Result: Didnt work (FOKI= 6,28 ng/µl; SPLIT=11,43 |
- | ng/µl; FOKA= 5,5 ng/µl) | + | ng/µl; FOKA= 5,5 ng/µl) </li> |
- | </li>
| + | |
| <li>We couldnt find plates with Lipocalin and Digoxigenin, | | <li>We couldnt find plates with Lipocalin and Digoxigenin, |
| inoculation was not possible | | inoculation was not possible |
- | -->Transformation has to be done tomorrow! | + | -->Transformation has to be done tomorrow! </li> |
- | </li>
| + | <li>Induced: 14: 15 BL21de3 with 1 ml of 1M IPTG </li> |
- | <li>Induced: 14: 15 BL21de3 with 1 ml of 1M IPTG | + | |
- | </li>
| + | |
| <li>Centrifuged BL21 De3 at 21:50. Pellets in the -20°C | | <li>Centrifuged BL21 De3 at 21:50. Pellets in the -20°C |
- | freezer | + | freezer </li> |
- | </li>
| + | <li>Induced T7 at 23:35 with 1 ml of 1M IPTG </li> |
- | <li>Induced T7 at 23:35 with 1 ml of 1M IPTG | + | |
- | </li>
| + | |
| <li><b>Nanodrop data</b></li> | | <li><b>Nanodrop data</b></li> |
| </ul> | | </ul> |
Line 1,652: |
Line 1,585: |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl636339" style="height: 15pt; width: 107pt;" | | <td class="xl636339" style="height: 15pt; width: 107pt;" |
- | height="20" width="143">Sample | + | height="20" width="143"><small>Sample |
- | ID</td> | + | ID</small></td> |
| <td class="xl636339" style="width: 48pt;" | | <td class="xl636339" style="width: 48pt;" |
- | width="64">User ID</td> | + | width="64"><small>User ID</small></td> |
| <td class="xl636339" style="width: 53pt;" | | <td class="xl636339" style="width: 53pt;" |
- | width="71">Date<span style=""> </span></td> | + | width="71"><small>Date<span style=""> </span></small></td> |
| <td class="xl636339" style="width: 48pt;" | | <td class="xl636339" style="width: 48pt;" |
- | width="64">Time<span style=""> </span></td> | + | width="64"><small>Time<span style=""> </span></small></td> |
| <td class="xl636339" style="width: 48pt;" | | <td class="xl636339" style="width: 48pt;" |
- | width="64">ng/ul<span style=""> </span></td> | + | width="64"><small>ng/ul<span style=""> </span></small></td> |
| <td class="xl636339" style="width: 48pt;" | | <td class="xl636339" style="width: 48pt;" |
- | width="64">A260<span style=""> </span></td> | + | width="64"><small>A260<span style=""> </span></small></td> |
| <td class="xl636339" style="width: 66pt;" | | <td class="xl636339" style="width: 66pt;" |
- | width="88">A280<span style=""> </span></td> | + | width="88"><small>A280<span style=""> </span></small></td> |
| <td class="xl636339" style="width: 71pt;" | | <td class="xl636339" style="width: 71pt;" |
- | width="94">260/280<span style=""> </span></td> | + | width="94"><small>260/280<span style=""> </span></small></td> |
| <td class="xl636339" style="width: 95pt;" | | <td class="xl636339" style="width: 95pt;" |
- | width="126">260/230<span style=""> </span></td> | + | width="126"><small>260/230<span style=""> |
| + | </span></small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl156339" style="height: 15pt;" | | <td class="xl156339" style="height: 15pt;" |
- | height="20">His-Tag 1</td> | + | height="20"><small>His-Tag 1</small></td> |
- | <td class="xl646339">FreiGEM</td> | + | <td class="xl646339"><small>FreiGEM</small></td> |
- | <td class="xl656339">14.07.2009</td> | + | <td class="xl656339"><small>14.07.2009</small></td> |
- | <td class="xl666339">10:56</td> | + | <td class="xl666339"><small>10:56</small></td> |
- | <td class="xl646339">228.22</td> | + | <td class="xl646339"><small>228.22</small></td> |
- | <td class="xl646339">4.564</td> | + | <td class="xl646339"><small>4.564</small></td> |
- | <td class="xl646339">2.381</td> | + | <td class="xl646339"><small>2.381</small></td> |
- | <td class="xl646339">1.92</td> | + | <td class="xl646339"><small>1.92</small></td> |
- | <td class="xl646339">2.18</td> | + | <td class="xl646339"><small>2.18</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl156339" style="height: 15pt;" | | <td class="xl156339" style="height: 15pt;" |
- | height="20">His-Tag 2</td> | + | height="20"><small>His-Tag 2</small></td> |
- | <td class="xl646339">FreiGEM</td> | + | <td class="xl646339"><small>FreiGEM</small></td> |
- | <td class="xl656339">14.07.2009</td> | + | <td class="xl656339"><small>14.07.2009</small></td> |
- | <td class="xl666339">10:58</td> | + | <td class="xl666339"><small>10:58</small></td> |
- | <td class="xl646339">227.96</td> | + | <td class="xl646339"><small>227.96</small></td> |
- | <td class="xl646339">4.559</td> | + | <td class="xl646339"><small>4.559</small></td> |
- | <td class="xl646339">2.385</td> | + | <td class="xl646339"><small>2.385</small></td> |
- | <td class="xl646339">1.91</td> | + | <td class="xl646339"><small>1.91</small></td> |
- | <td class="xl646339">2.19</td> | + | <td class="xl646339"><small>2.19</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl156339" style="height: 15pt;" | | <td class="xl156339" style="height: 15pt;" |
- | height="20">Strep-Tag 1</td> | + | height="20"><small>Strep-Tag 1</small></td> |
- | <td class="xl646339">FreiGEM</td> | + | <td class="xl646339"><small>FreiGEM</small></td> |
- | <td class="xl656339">14.07.2009</td> | + | <td class="xl656339"><small>14.07.2009</small></td> |
- | <td class="xl666339">10:59</td> | + | <td class="xl666339"><small>10:59</small></td> |
- | <td class="xl646339">241.42</td> | + | <td class="xl646339"><small>241.42</small></td> |
- | <td class="xl646339">4.828</td> | + | <td class="xl646339"><small>4.828</small></td> |
- | <td class="xl646339">2.55</td> | + | <td class="xl646339"><small>2.55</small></td> |
- | <td class="xl646339">1.89</td> | + | <td class="xl646339"><small>1.89</small></td> |
- | <td class="xl646339">2.1</td> | + | <td class="xl646339"><small>2.1</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl156339" style="height: 15pt;" | | <td class="xl156339" style="height: 15pt;" |
- | height="20">Strep-Tag 1</td> | + | height="20"><small>Strep-Tag 1</small></td> |
- | <td class="xl646339">FreiGEM</td> | + | <td class="xl646339"><small>FreiGEM</small></td> |
- | <td class="xl656339">14.07.2009</td> | + | <td class="xl656339"><small>14.07.2009</small></td> |
- | <td class="xl666339">11:00</td> | + | <td class="xl666339"><small>11:00</small></td> |
- | <td class="xl646339">259.14</td> | + | <td class="xl646339"><small>259.14</small></td> |
- | <td class="xl646339">5.183</td> | + | <td class="xl646339"><small>5.183</small></td> |
- | <td class="xl646339">2.782</td> | + | <td class="xl646339"><small>2.782</small></td> |
- | <td class="xl646339">1.86</td> | + | <td class="xl646339"><small>1.86</small></td> |
- | <td class="xl646339">1.75</td> | + | <td class="xl646339"><small>1.75</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl156339" style="height: 15pt;" | | <td class="xl156339" style="height: 15pt;" |
- | height="20">Split-Linker 1</td> | + | height="20"><small>Split-Linker 1</small></td> |
- | <td class="xl646339">FreiGEM</td> | + | <td class="xl646339"><small>FreiGEM</small></td> |
- | <td class="xl656339">14.07.2009</td> | + | <td class="xl656339"><small>14.07.2009</small></td> |
- | <td class="xl666339">11:01</td> | + | <td class="xl666339"><small>11:01</small></td> |
- | <td class="xl646339">261.65</td> | + | <td class="xl646339"><small>261.65</small></td> |
- | <td class="xl646339">5.233</td> | + | <td class="xl646339"><small>5.233</small></td> |
- | <td class="xl646339">2.746</td> | + | <td class="xl646339"><small>2.746</small></td> |
- | <td class="xl646339">1.91</td> | + | <td class="xl646339"><small>1.91</small></td> |
- | <td class="xl646339">2.14</td> | + | <td class="xl646339"><small>2.14</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl156339" style="height: 15pt;" | | <td class="xl156339" style="height: 15pt;" |
- | height="20">Split-Linker 2</td> | + | height="20"><small>Split-Linker 2</small></td> |
- | <td class="xl646339">FreiGEM</td> | + | <td class="xl646339"><small>FreiGEM</small></td> |
- | <td class="xl656339">14.07.2009</td> | + | <td class="xl656339"><small>14.07.2009</small></td> |
- | <td class="xl666339">11:02</td> | + | <td class="xl666339"><small>11:02</small></td> |
- | <td class="xl646339">282.13</td> | + | <td class="xl646339"><small>282.13</small></td> |
- | <td class="xl646339">5.643</td> | + | <td class="xl646339"><small>5.643</small></td> |
- | <td class="xl646339">2.989</td> | + | <td class="xl646339"><small>2.989</small></td> |
- | <td class="xl646339">1.89</td> | + | <td class="xl646339"><small>1.89</small></td> |
- | <td class="xl646339">2</td> | + | <td class="xl646339"><small>2</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl156339" style="height: 15pt;" | | <td class="xl156339" style="height: 15pt;" |
- | height="20">Lig. 8.7. 100 µl 1</td> | + | height="20"><small>Lig. 8.7. 100 µl 1</small></td> |
- | <td class="xl646339">FreiGEM</td> | + | <td class="xl646339"><small>FreiGEM</small></td> |
- | <td class="xl656339">14.07.2009</td> | + | <td class="xl656339"><small>14.07.2009</small></td> |
- | <td class="xl666339">11:03</td> | + | <td class="xl666339"><small>11:03</small></td> |
- | <td class="xl646339">117.34</td> | + | <td class="xl646339"><small>117.34</small></td> |
- | <td class="xl646339">2.347</td> | + | <td class="xl646339"><small>2.347</small></td> |
- | <td class="xl646339">1.241</td> | + | <td class="xl646339"><small>1.241</small></td> |
- | <td class="xl646339">1.89</td> | + | <td class="xl646339"><small>1.89</small></td> |
- | <td class="xl646339">1.81</td> | + | <td class="xl646339"><small>1.81</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl156339" style="height: 15pt;" | | <td class="xl156339" style="height: 15pt;" |
- | height="20">Lig. 8.7. 100 µl 1</td> | + | height="20"><small>Lig. 8.7. 100 µl 1</small></td> |
- | <td class="xl646339">FreiGEM</td> | + | <td class="xl646339"><small>FreiGEM</small></td> |
- | <td class="xl656339">14.07.2009</td> | + | <td class="xl656339"><small>14.07.2009</small></td> |
- | <td class="xl666339">11:04</td> | + | <td class="xl666339"><small>11:04</small></td> |
- | <td class="xl646339">75.98</td> | + | <td class="xl646339"><small>75.98</small></td> |
- | <td class="xl646339">1.52</td> | + | <td class="xl646339"><small>1.52</small></td> |
- | <td class="xl646339">0.823</td> | + | <td class="xl646339"><small>0.823</small></td> |
- | <td class="xl646339">1.85</td> | + | <td class="xl646339"><small>1.85</small></td> |
- | <td class="xl646339">1.42</td> | + | <td class="xl646339"><small>1.42</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl156339" style="height: 15pt;" | | <td class="xl156339" style="height: 15pt;" |
- | height="20">Lig. 8.7. Rest µl 1</td> | + | height="20"><small>Lig. 8.7. Rest µl 1</small></td> |
- | <td class="xl646339">FreiGEM</td> | + | <td class="xl646339"><small>FreiGEM</small></td> |
- | <td class="xl656339">14.07.2009</td> | + | <td class="xl656339"><small>14.07.2009</small></td> |
- | <td class="xl666339">11:05</td> | + | <td class="xl666339"><small>11:05</small></td> |
- | <td class="xl646339">154.78</td> | + | <td class="xl646339"><small>154.78</small></td> |
- | <td class="xl646339">3.096</td> | + | <td class="xl646339"><small>3.096</small></td> |
- | <td class="xl646339">1.652</td> | + | <td class="xl646339"><small>1.652</small></td> |
- | <td class="xl646339">1.87</td> | + | <td class="xl646339"><small>1.87</small></td> |
- | <td class="xl646339">1.59</td> | + | <td class="xl646339"><small>1.59</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl156339" style="height: 15pt;" | | <td class="xl156339" style="height: 15pt;" |
- | height="20">Lig. 8.7. Rest µl 2</td> | + | height="20"><small>Lig. 8.7. Rest µl 2</small></td> |
- | <td class="xl646339">FreiGEM</td> | + | <td class="xl646339"><small>FreiGEM</small></td> |
- | <td class="xl656339">14.07.2009</td> | + | <td class="xl656339"><small>14.07.2009</small></td> |
- | <td class="xl666339">11:06</td> | + | <td class="xl666339"><small>11:06</small></td> |
- | <td class="xl646339">129.27</td> | + | <td class="xl646339"><small>129.27</small></td> |
- | <td class="xl646339">2.585</td> | + | <td class="xl646339"><small>2.585</small></td> |
- | <td class="xl646339">1.344</td> | + | <td class="xl646339"><small>1.344</small></td> |
- | <td class="xl646339">1.92</td> | + | <td class="xl646339"><small>1.92</small></td> |
- | <td class="xl646339">1.8</td> | + | <td class="xl646339"><small>1.8</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl156339" style="height: 15pt;" | | <td class="xl156339" style="height: 15pt;" |
- | height="20">Lig. 8.7. +IPTG 100 µl 1</td> | + | height="20"><small>Lig. 8.7. +IPTG 100 µl 1</small></td> |
- | <td class="xl646339">FreiGEM</td> | + | <td class="xl646339"><small>FreiGEM</small></td> |
- | <td class="xl656339">14.07.2009</td> | + | <td class="xl656339"><small>14.07.2009</small></td> |
- | <td class="xl666339">11:08</td> | + | <td class="xl666339"><small>11:08</small></td> |
- | <td class="xl646339">141.95</td> | + | <td class="xl646339"><small>141.95</small></td> |
- | <td class="xl646339">2.839</td> | + | <td class="xl646339"><small>2.839</small></td> |
- | <td class="xl646339">1.452</td> | + | <td class="xl646339"><small>1.452</small></td> |
- | <td class="xl646339">1.95</td> | + | <td class="xl646339"><small>1.95</small></td> |
- | <td class="xl646339">1.7</td> | + | <td class="xl646339"><small>1.7</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl156339" style="height: 15pt;" | | <td class="xl156339" style="height: 15pt;" |
- | height="20">Lig. 8.7. +IPTG 100 µl 2</td> | + | height="20"><small>Lig. 8.7. +IPTG 100 µl 2</small></td> |
- | <td class="xl646339">FreiGEM</td> | + | <td class="xl646339"><small>FreiGEM</small></td> |
- | <td class="xl656339">14.07.2009</td> | + | <td class="xl656339"><small>14.07.2009</small></td> |
- | <td class="xl666339">11:09</td> | + | <td class="xl666339"><small>11:09</small></td> |
- | <td class="xl646339">127.58</td> | + | <td class="xl646339"><small>127.58</small></td> |
- | <td class="xl646339">2.552</td> | + | <td class="xl646339"><small>2.552</small></td> |
- | <td class="xl646339">1.363</td> | + | <td class="xl646339"><small>1.363</small></td> |
- | <td class="xl646339">1.87</td> | + | <td class="xl646339"><small>1.87</small></td> |
- | <td class="xl646339">1.83</td> | + | <td class="xl646339"><small>1.83</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl156339" style="height: 15pt;" | | <td class="xl156339" style="height: 15pt;" |
- | height="20">Lig. 9.7. alte</td> | + | height="20"><small>Lig. 9.7. alte</small></td> |
- | <td class="xl646339">FreiGEM</td> | + | <td class="xl646339"><small>FreiGEM</small></td> |
- | <td class="xl656339">14.07.2009</td> | + | <td class="xl656339"><small>14.07.2009</small></td> |
- | <td class="xl666339">11:10</td> | + | <td class="xl666339"><small>11:10</small></td> |
- | <td class="xl646339">136.49</td> | + | <td class="xl646339"><small>136.49</small></td> |
- | <td class="xl646339">2.73</td> | + | <td class="xl646339"><small>2.73</small></td> |
- | <td class="xl646339">1.419</td> | + | <td class="xl646339"><small>1.419</small></td> |
- | <td class="xl646339">1.92</td> | + | <td class="xl646339"><small>1.92</small></td> |
- | <td class="xl646339">1.84</td> | + | <td class="xl646339"><small>1.84</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl156339" style="height: 15pt;" | | <td class="xl156339" style="height: 15pt;" |
- | height="20">Lig. 9.7. neu2 1</td> | + | height="20"><small>Lig. 9.7. neu2 1</small></td> |
- | <td class="xl646339">FreiGEM</td> | + | <td class="xl646339"><small>FreiGEM</small></td> |
- | <td class="xl656339">14.07.2009</td> | + | <td class="xl656339"><small>14.07.2009</small></td> |
- | <td class="xl666339">11:11</td> | + | <td class="xl666339"><small>11:11</small></td> |
- | <td class="xl646339">151.55</td> | + | <td class="xl646339"><small>151.55</small></td> |
- | <td class="xl646339">3.031</td> | + | <td class="xl646339"><small>3.031</small></td> |
- | <td class="xl646339">1.61</td> | + | <td class="xl646339"><small>1.61</small></td> |
- | <td class="xl646339">1.88</td> | + | <td class="xl646339"><small>1.88</small></td> |
- | <td class="xl646339">1.55</td> | + | <td class="xl646339"><small>1.55</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl156339" style="height: 15pt;" | | <td class="xl156339" style="height: 15pt;" |
- | height="20">Lig. 9.7. neu2 2</td> | + | height="20"><small>Lig. 9.7. neu2 2</small></td> |
- | <td class="xl646339">FreiGEM</td> | + | <td class="xl646339"><small>FreiGEM</small></td> |
- | <td class="xl656339">14.07.2009</td> | + | <td class="xl656339"><small>14.07.2009</small></td> |
- | <td class="xl666339">11:12</td> | + | <td class="xl666339"><small>11:12</small></td> |
- | <td class="xl646339">138.65</td> | + | <td class="xl646339"><small>138.65</small></td> |
- | <td class="xl646339">2.773</td> | + | <td class="xl646339"><small>2.773</small></td> |
- | <td class="xl646339">1.448</td> | + | <td class="xl646339"><small>1.448</small></td> |
- | <td class="xl646339">1.92</td> | + | <td class="xl646339"><small>1.92</small></td> |
- | <td class="xl646339">1.83</td> | + | <td class="xl646339"><small>1.83</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl156339" style="height: 15pt;" | | <td class="xl156339" style="height: 15pt;" |
- | height="20">FOKI 14/07/09</td> | + | height="20"><small>FOKI 14/07/09</small></td> |
- | <td class="xl646339">FreiGEM</td> | + | <td class="xl646339"><small>FreiGEM</small></td> |
- | <td class="xl656339">14.07.2009</td> | + | <td class="xl656339"><small>14.07.2009</small></td> |
- | <td class="xl666339">20:39</td> | + | <td class="xl666339"><small>20:39</small></td> |
- | <td class="xl646339">6.28</td> | + | <td class="xl646339"><small>6.28</small></td> |
- | <td class="xl646339">0.126</td> | + | <td class="xl646339"><small>0.126</small></td> |
- | <td class="xl646339">0.067</td> | + | <td class="xl646339"><small>0.067</small></td> |
- | <td class="xl646339">1.87</td> | + | <td class="xl646339"><small>1.87</small></td> |
- | <td class="xl646339">0.03</td> | + | <td class="xl646339"><small>0.03</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl156339" style="height: 15pt;" | | <td class="xl156339" style="height: 15pt;" |
- | height="20">split</td> | + | height="20"><small>split</small></td> |
- | <td class="xl646339">FreiGEM</td> | + | <td class="xl646339"><small>FreiGEM</small></td> |
- | <td class="xl656339">14.07.2009</td> | + | <td class="xl656339"><small>14.07.2009</small></td> |
- | <td class="xl666339">20:42</td> | + | <td class="xl666339"><small>20:42</small></td> |
- | <td class="xl646339">11.43</td> | + | <td class="xl646339"><small>11.43</small></td> |
- | <td class="xl646339">0.229</td> | + | <td class="xl646339"><small>0.229</small></td> |
- | <td class="xl646339">0.155</td> | + | <td class="xl646339"><small>0.155</small></td> |
- | <td class="xl646339">1.48</td> | + | <td class="xl646339"><small>1.48</small></td> |
- | <td class="xl646339">0.06</td> | + | <td class="xl646339"><small>0.06</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl156339" style="height: 15pt;" | | <td class="xl156339" style="height: 15pt;" |
- | height="20">FokA</td> | + | height="20"><small>FokA</small></td> |
- | <td class="xl646339">FreiGEM</td> | + | <td class="xl646339"><small>FreiGEM</small></td> |
- | <td class="xl656339">14.07.2009</td> | + | <td class="xl656339"><small>14.07.2009</small></td> |
- | <td class="xl666339">20:46</td> | + | <td class="xl666339"><small>20:46</small></td> |
- | <td class="xl646339">5.5</td> | + | <td class="xl646339"><small>5.5</small></td> |
- | <td class="xl646339">0.11</td> | + | <td class="xl646339"><small>0.11</small></td> |
- | <td class="xl646339">0.068</td> | + | <td class="xl646339"><small>0.068</small></td> |
- | <td class="xl646339">1.61</td> | + | <td class="xl646339"><small>1.61</small></td> |
- | <td class="xl646339">0.02</td> | + | <td class="xl646339"><small>0.02</small></td> |
| </tr> | | </tr> |
| <tr style="display: none;" height="0"> | | <tr style="display: none;" height="0"> |
Line 1,914: |
Line 1,848: |
| <li>-new digests: Fok, Split, Lipo, Dig, His(?), Strep(?), | | <li>-new digests: Fok, Split, Lipo, Dig, His(?), Strep(?), |
| ligation(?)</li> | | ligation(?)</li> |
- | <li>-purification of AGO with His/Ni column | + | <li>-purification of AGO with His/Ni column </li> |
- | </li>
| + | |
| </ul> | | </ul> |
| </ul> | | </ul> |
Line 1,921: |
Line 1,854: |
| <br /> | | <br /> |
| <ul> | | <ul> |
- | <li>digestion of Split, FOKa and FOKi + gelextraction | + | <li>digestion of Split, FOKa and FOKi + gelextraction </li> |
- | </li>
| + | <li>sequenzing of Lig 8.7. "neu, alt, 100" </li> |
- | <li>sequenzing of Lig 8.7. "neu, alt, 100" | + | |
- | </li>
| + | |
| <li>run agarose gel of the digestion and cut the bands, did a | | <li>run agarose gel of the digestion and cut the bands, did a |
| gel extraction and measured the concentration at the NanoDrop. The | | gel extraction and measured the concentration at the NanoDrop. The |
| concentration was again too low, to do a ligation. Put the samples in | | concentration was again too low, to do a ligation. Put the samples in |
- | the -20°C freezer. | + | the -20°C freezer. </li> |
- | </li>
| + | |
| </ul> | | </ul> |
| <ul> | | <ul> |
Line 1,941: |
Line 1,871: |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl65345" style="height: 15pt; width: 107pt;" | | <td class="xl65345" style="height: 15pt; width: 107pt;" |
- | height="20" width="143">Sample | + | height="20" width="143"><small>Sample |
- | ID</td> | + | ID</small></td> |
| <td class="xl65345" style="width: 48pt;" | | <td class="xl65345" style="width: 48pt;" |
- | width="64">User ID</td> | + | width="64"><small>User ID</small></td> |
| <td class="xl65345" style="width: 53pt;" | | <td class="xl65345" style="width: 53pt;" |
- | width="71">Date<span style=""> </span></td> | + | width="71"><small>Date<span style=""> </span></small></td> |
| <td class="xl65345" style="width: 48pt;" | | <td class="xl65345" style="width: 48pt;" |
- | width="64">Time<span style=""> </span></td> | + | width="64"><small>Time<span style=""> </span></small></td> |
| <td class="xl65345" style="width: 48pt;" | | <td class="xl65345" style="width: 48pt;" |
- | width="64">ng/ul<span style=""> </span></td> | + | width="64"><small>ng/ul<span style=""> </span></small></td> |
| <td class="xl65345" style="width: 48pt;" | | <td class="xl65345" style="width: 48pt;" |
- | width="64">A260<span style=""> </span></td> | + | width="64"><small>A260<span style=""> </span></small></td> |
| <td class="xl65345" style="width: 66pt;" | | <td class="xl65345" style="width: 66pt;" |
- | width="88">A280<span style=""> </span></td> | + | width="88"><small>A280<span style=""> </span></small></td> |
| <td class="xl65345" style="width: 71pt;" | | <td class="xl65345" style="width: 71pt;" |
- | width="94">260/280<span style=""> </span></td> | + | width="94"><small>260/280<span style=""> </span></small></td> |
| <td class="xl65345" style="width: 95pt;" | | <td class="xl65345" style="width: 95pt;" |
- | width="126">260/230<span style=""> </span></td> | + | width="126"><small>260/230<span style=""> |
| + | </span></small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl15345" style="height: 15pt;" | | <td class="xl15345" style="height: 15pt;" |
- | height="20">Split-Linker</td> | + | height="20"><small>Split-Linker</small></td> |
- | <td class="xl15345">FreiGEM</td> | + | <td class="xl15345"><small>FreiGEM</small></td> |
- | <td class="xl63345" align="right">16.07.2009</td> | + | <td class="xl63345" align="right"><small>16.07.2009</small></td> |
- | <td class="xl64345" align="right">17:21</td> | + | <td class="xl64345" align="right"><small>17:21</small></td> |
- | <td class="xl15345" align="right">30.02</td> | + | <td class="xl15345" align="right"><small>30.02</small></td> |
- | <td class="xl15345" align="right">0.6</td> | + | <td class="xl15345" align="right"><small>0.6</small></td> |
- | <td class="xl15345" align="right">0.277</td> | + | <td class="xl15345" align="right"><small>0.277</small></td> |
- | <td class="xl15345" align="right">2.17</td> | + | <td class="xl15345" align="right"><small>2.17</small></td> |
- | <td class="xl15345" align="right">0.53</td> | + | <td class="xl15345" align="right"><small>0.53</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl15345" style="height: 15pt;" | | <td class="xl15345" style="height: 15pt;" |
- | height="20">FokI active</td> | + | height="20"><small>FokI active</small></td> |
- | <td class="xl15345">FreiGEM</td> | + | <td class="xl15345"><small>FreiGEM</small></td> |
- | <td class="xl63345" align="right">16.07.2009</td> | + | <td class="xl63345" align="right"><small>16.07.2009</small></td> |
- | <td class="xl64345" align="right">17:22</td> | + | <td class="xl64345" align="right"><small>17:22</small></td> |
- | <td class="xl15345" align="right">2.29</td> | + | <td class="xl15345" align="right"><small>2.29</small></td> |
- | <td class="xl15345" align="right">0.046</td> | + | <td class="xl15345" align="right"><small>0.046</small></td> |
- | <td class="xl15345" align="right">-0.004</td> | + | <td class="xl15345" align="right"><small>-0.004</small></td> |
- | <td class="xl15345" align="right">-11.17</td> | + | <td class="xl15345" align="right"><small>-11.17</small></td> |
- | <td class="xl15345" align="right">0.02</td> | + | <td class="xl15345" align="right"><small>0.02</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl15345" style="height: 15pt;" | | <td class="xl15345" style="height: 15pt;" |
- | height="20">FokI active</td> | + | height="20"><small>FokI active</small></td> |
- | <td class="xl15345">FreiGEM</td> | + | <td class="xl15345"><small>FreiGEM</small></td> |
- | <td class="xl63345" align="right">16.07.2009</td> | + | <td class="xl63345" align="right"><small>16.07.2009</small></td> |
- | <td class="xl64345" align="right">17:23</td> | + | <td class="xl64345" align="right"><small>17:23</small></td> |
- | <td class="xl15345" align="right">42.13</td> | + | <td class="xl15345" align="right"><small>42.13</small></td> |
- | <td class="xl15345" align="right">0.843</td> | + | <td class="xl15345" align="right"><small>0.843</small></td> |
- | <td class="xl15345" align="right">0.488</td> | + | <td class="xl15345" align="right"><small>0.488</small></td> |
- | <td class="xl15345" align="right">1.73</td> | + | <td class="xl15345" align="right"><small>1.73</small></td> |
- | <td class="xl15345" align="right">0.44</td> | + | <td class="xl15345" align="right"><small>0.44</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl15345" style="height: 15pt;" | | <td class="xl15345" style="height: 15pt;" |
- | height="20">Blank</td> | + | height="20"><small>Blank</small></td> |
- | <td class="xl15345">FreiGEM</td> | + | <td class="xl15345"><small>FreiGEM</small></td> |
- | <td class="xl63345" align="right">16.07.2009</td> | + | <td class="xl63345" align="right"><small>16.07.2009</small></td> |
- | <td class="xl64345" align="right">17:30</td> | + | <td class="xl64345" align="right"><small>17:30</small></td> |
- | <td class="xl15345" align="right">0.64</td> | + | <td class="xl15345" align="right"><small>0.64</small></td> |
- | <td class="xl15345" align="right">0.013</td> | + | <td class="xl15345" align="right"><small>0.013</small></td> |
- | <td class="xl15345" align="right">0.004</td> | + | <td class="xl15345" align="right"><small>0.004</small></td> |
- | <td class="xl15345" align="right">3.5</td> | + | <td class="xl15345" align="right"><small>3.5</small></td> |
- | <td class="xl15345" align="right">0.85</td> | + | <td class="xl15345" align="right"><small>0.85</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl15345" style="height: 15pt;" | | <td class="xl15345" style="height: 15pt;" |
- | height="20">Split-Linker 2</td> | + | height="20"><small>Split-Linker 2</small></td> |
- | <td class="xl15345">FreiGEM</td> | + | <td class="xl15345"><small>FreiGEM</small></td> |
- | <td class="xl63345" align="right">16.07.2009</td> | + | <td class="xl63345" align="right"><small>16.07.2009</small></td> |
- | <td class="xl64345" align="right">17:32</td> | + | <td class="xl64345" align="right"><small>17:32</small></td> |
- | <td class="xl15345" align="right">8.6</td> | + | <td class="xl15345" align="right"><small>8.6</small></td> |
- | <td class="xl15345" align="right">0.172</td> | + | <td class="xl15345" align="right"><small>0.172</small></td> |
- | <td class="xl15345" align="right">0.082</td> | + | <td class="xl15345" align="right"><small>0.082</small></td> |
- | <td class="xl15345" align="right">2.1</td> | + | <td class="xl15345" align="right"><small>2.1</small></td> |
- | <td class="xl15345" align="right">0.66</td> | + | <td class="xl15345" align="right"><small>0.66</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl15345" style="height: 15pt;" | | <td class="xl15345" style="height: 15pt;" |
- | height="20">FokI active</td> | + | height="20"><small>FokI active</small></td> |
- | <td class="xl15345">FreiGEM</td> | + | <td class="xl15345"><small>FreiGEM</small></td> |
- | <td class="xl63345" align="right">16.07.2009</td> | + | <td class="xl63345" align="right"><small>16.07.2009</small></td> |
- | <td class="xl64345" align="right">17:33</td> | + | <td class="xl64345" align="right"><small>17:33</small></td> |
- | <td class="xl15345" align="right">12.64</td> | + | <td class="xl15345" align="right"><small>12.64</small></td> |
- | <td class="xl15345" align="right">0.253</td> | + | <td class="xl15345" align="right"><small>0.253</small></td> |
- | <td class="xl15345" align="right">0.161</td> | + | <td class="xl15345" align="right"><small>0.161</small></td> |
- | <td class="xl15345" align="right">1.57</td> | + | <td class="xl15345" align="right"><small>1.57</small></td> |
- | <td class="xl15345" align="right">0.26</td> | + | <td class="xl15345" align="right"><small>0.26</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl15345" style="height: 15pt;" | | <td class="xl15345" style="height: 15pt;" |
- | height="20">FokI inactive 2</td> | + | height="20"><small>FokI inactive 2</small></td> |
- | <td class="xl15345">FreiGEM</td> | + | <td class="xl15345"><small>FreiGEM</small></td> |
- | <td class="xl63345" align="right">16.07.2009</td> | + | <td class="xl63345" align="right"><small>16.07.2009</small></td> |
- | <td class="xl64345" align="right">17:33</td> | + | <td class="xl64345" align="right"><small>17:33</small></td> |
- | <td class="xl15345" align="right">8.68</td> | + | <td class="xl15345" align="right"><small>8.68</small></td> |
- | <td class="xl15345" align="right">0.174</td> | + | <td class="xl15345" align="right"><small>0.174</small></td> |
- | <td class="xl15345" align="right">0.107</td> | + | <td class="xl15345" align="right"><small>0.107</small></td> |
- | <td class="xl15345" align="right">1.63</td> | + | <td class="xl15345" align="right"><small>1.63</small></td> |
- | <td class="xl15345" align="right">0.44</td> | + | <td class="xl15345" align="right"><small>0.44</small></td> |
| </tr> | | </tr> |
| <tr style="display: none;" height="0"> | | <tr style="display: none;" height="0"> |
Line 2,060: |
Line 1,991: |
| <br /> | | <br /> |
| <ul> | | <ul> |
- | <li>5 l LBmedia prepared | + | <li>5 l LBmedia prepared </li> |
- | </li>
| + | |
| <li> we got a look at the ssDNA of the M13 phage, | | <li> we got a look at the ssDNA of the M13 phage, |
| there is no Fok restriction site. </li> | | there is no Fok restriction site. </li> |
| <li> we also made an alignment of the pMAL+CAT vector | | <li> we also made an alignment of the pMAL+CAT vector |
- | and the parts we got back from sequencing. the 2.1 clone is faultless. | + | and the parts we got back from sequencing. the 2.1 clone is faultless. </li> |
- | </li>
| + | |
| <li>His-Tag purification of Tt and Aa AGOs via Ni-NTA column | | <li>His-Tag purification of Tt and Aa AGOs via Ni-NTA column |
| -> SDS Gel and protein concentration measurement needs to be | | -> SDS Gel and protein concentration measurement needs to be |
- | done on monday (all samples in the -20°C freezer!) | + | done on monday (all samples in the -20°C freezer!) </li> |
- | </li>
| + | |
| </ul> | | </ul> |
| <h3>20.07.09, Gerrit + Imi</h3> | | <h3>20.07.09, Gerrit + Imi</h3> |
Line 2,083: |
Line 2,011: |
| height="250" width="230" /> | | height="250" width="230" /> |
| <br /> | | <br /> |
- | Aa on the upper gel with M,W2,W3,W4,E3,E1,E4,E2,E6,E5; then Tt on the | + | <small>Aa on the upper gel with M,W2,W3,W4,E3,E1,E4,E2,E6,E5; |
| + | then Tt on the |
| lower gel with M,W2,W3,W4,E1,E2,E3,E4,E5,E6 | | lower gel with M,W2,W3,W4,E1,E2,E3,E4,E5,E6 |
- | <br /> | + | </small><br /> |
| <ul> | | <ul> |
- | <li>Ligation of FokA + Split, FokI + Split | + | <li>Ligation of FokA + Split, FokI + Split </li> |
- | </li>
| + | <li>Transformations of FokA+Split, FokI+Split, Dig, Lipo, GGGS </li> |
- | <li>Transformations of FokA+Split, FokI+Split, Dig, Lipo, GGGS | + | <li>Transformation of pUC: proliferation of the pUC-vector </li> |
- | </li>
| + | |
- | <li>Transformation of pUC: proliferation of the pUC-vector | + | |
- | </li>
| + | |
| <li>Inoculation of FokI, FokA, neu1, neu2, Aa vector, Tt vector | | <li>Inoculation of FokI, FokA, neu1, neu2, Aa vector, Tt vector |
| in LB+Amp in order to create stocks since the cold storage room is | | in LB+Amp in order to create stocks since the cold storage room is |
- | out!! | + | out!! </li> |
- | </li>
| + | |
| <li><b>Nanodrop data</b></li> | | <li><b>Nanodrop data</b></li> |
| </ul> | | </ul> |
Line 2,106: |
Line 2,031: |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl1526463" style="height: 15pt; width: 62pt;" | | <td class="xl1526463" style="height: 15pt; width: 62pt;" |
- | height="20" width="82">Sample | + | height="20" width="82"><small>Sample |
- | ID</td> | + | ID</small></td> |
| <td class="xl1526463" style="width: 48pt;" | | <td class="xl1526463" style="width: 48pt;" |
- | width="64">User ID</td> | + | width="64"><small>User ID</small></td> |
| <td class="xl1526463" style="width: 53pt;" | | <td class="xl1526463" style="width: 53pt;" |
- | width="71">Date<span style=""> </span></td> | + | width="71"><small>Date<span style=""> </span></small></td> |
| <td class="xl1526463" style="width: 48pt;" | | <td class="xl1526463" style="width: 48pt;" |
- | width="64">Time<span style=""> </span></td> | + | width="64"><small>Time<span style=""> </span></small></td> |
| <td class="xl1526463" style="width: 48pt;" | | <td class="xl1526463" style="width: 48pt;" |
- | width="64">mg/ml<span style=""> </span></td> | + | width="64"><small>mg/ml<span style=""> </span></small></td> |
| <td class="xl1526463" style="width: 48pt;" | | <td class="xl1526463" style="width: 48pt;" |
- | width="64">260/280<span style=""> </span></td> | + | width="64"><small>260/280<span style=""> </span></small></td> |
| <td class="xl1526463" style="width: 66pt;" | | <td class="xl1526463" style="width: 66pt;" |
- | width="88">A280 10mm</td> | + | width="88"><small>A280 10mm</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl1526463" style="height: 15pt;" | | <td class="xl1526463" style="height: 15pt;" |
- | height="20">T-W1</td> | + | height="20"><small>T-W1</small></td> |
- | <td class="xl1526463">FreiGEM</td> | + | <td class="xl1526463"><small>FreiGEM</small></td> |
- | <td class="xl6326463" align="right">20.07.2009</td> | + | <td class="xl6326463" align="right"><small>20.07.2009</small></td> |
- | <td class="xl6426463" align="right">14:10</td> | + | <td class="xl6426463" align="right"><small>14:10</small></td> |
- | <td class="xl1526463" align="right">-0.64</td> | + | <td class="xl1526463" align="right"><small>-0.64</small></td> |
- | <td class="xl1526463" align="right">1.37</td> | + | <td class="xl1526463" align="right"><small>1.37</small></td> |
- | <td class="xl1526463" align="right">-0.642</td> | + | <td class="xl1526463" align="right"><small>-0.642</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl1526463" style="height: 15pt;" | | <td class="xl1526463" style="height: 15pt;" |
- | height="20">T-W1</td> | + | height="20"><small>T-W1</small></td> |
- | <td class="xl1526463">FreiGEM</td> | + | <td class="xl1526463"><small>FreiGEM</small></td> |
- | <td class="xl6326463" align="right">20.07.2009</td> | + | <td class="xl6326463" align="right"><small>20.07.2009</small></td> |
- | <td class="xl6426463" align="right">14:11</td> | + | <td class="xl6426463" align="right"><small>14:11</small></td> |
- | <td class="xl1526463" align="right">22.59</td> | + | <td class="xl1526463" align="right"><small>22.59</small></td> |
- | <td class="xl1526463" align="right">1.96</td> | + | <td class="xl1526463" align="right"><small>1.96</small></td> |
- | <td class="xl1526463" align="right">22.59</td> | + | <td class="xl1526463" align="right"><small>22.59</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl1526463" style="height: 15pt;" | | <td class="xl1526463" style="height: 15pt;" |
- | height="20">T-W1</td> | + | height="20"><small>T-W1</small></td> |
- | <td class="xl1526463">FreiGEM</td> | + | <td class="xl1526463"><small>FreiGEM</small></td> |
- | <td class="xl6326463" align="right">20.07.2009</td> | + | <td class="xl6326463" align="right"><small>20.07.2009</small></td> |
- | <td class="xl6426463" align="right">14:16</td> | + | <td class="xl6426463" align="right"><small>14:16</small></td> |
- | <td class="xl1526463" align="right">-5.9</td> | + | <td class="xl1526463" align="right"><small>-5.9</small></td> |
- | <td class="xl1526463" align="right">1.98</td> | + | <td class="xl1526463" align="right"><small>1.98</small></td> |
- | <td class="xl1526463" align="right">-5.896</td> | + | <td class="xl1526463" align="right"><small>-5.896</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl1526463" style="height: 15pt;" | | <td class="xl1526463" style="height: 15pt;" |
- | height="20">T-W1</td> | + | height="20"><small>T-W1</small></td> |
- | <td class="xl1526463">FreiGEM</td> | + | <td class="xl1526463"><small>FreiGEM</small></td> |
- | <td class="xl6326463" align="right">20.07.2009</td> | + | <td class="xl6326463" align="right"><small>20.07.2009</small></td> |
- | <td class="xl6426463" align="right">14:17</td> | + | <td class="xl6426463" align="right"><small>14:17</small></td> |
- | <td class="xl1526463" align="right">24.06</td> | + | <td class="xl1526463" align="right"><small>24.06</small></td> |
- | <td class="xl1526463" align="right">1.97</td> | + | <td class="xl1526463" align="right"><small>1.97</small></td> |
- | <td class="xl1526463" align="right">24.057</td> | + | <td class="xl1526463" align="right"><small>24.057</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl1526463" style="height: 15pt;" | | <td class="xl1526463" style="height: 15pt;" |
- | height="20">T-W2</td> | + | height="20"><small>T-W2</small></td> |
- | <td class="xl1526463">FreiGEM</td> | + | <td class="xl1526463"><small>FreiGEM</small></td> |
- | <td class="xl6326463" align="right">20.07.2009</td> | + | <td class="xl6326463" align="right"><small>20.07.2009</small></td> |
- | <td class="xl6426463" align="right">14:18</td> | + | <td class="xl6426463" align="right"><small>14:18</small></td> |
- | <td class="xl1526463" align="right">-4.39</td> | + | <td class="xl1526463" align="right"><small>-4.39</small></td> |
- | <td class="xl1526463" align="right">2</td> | + | <td class="xl1526463" align="right"><small>2</small></td> |
- | <td class="xl1526463" align="right">-4.388</td> | + | <td class="xl1526463" align="right"><small>-4.388</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl1526463" style="height: 15pt;" | | <td class="xl1526463" style="height: 15pt;" |
- | height="20">T-W2</td> | + | height="20"><small>T-W2</small></td> |
- | <td class="xl1526463">FreiGEM</td> | + | <td class="xl1526463"><small>FreiGEM</small></td> |
- | <td class="xl6326463" align="right">20.07.2009</td> | + | <td class="xl6326463" align="right"><small>20.07.2009</small></td> |
- | <td class="xl6426463" align="right">14:19</td> | + | <td class="xl6426463" align="right"><small>14:19</small></td> |
- | <td class="xl1526463" align="right">18.92</td> | + | <td class="xl1526463" align="right"><small>18.92</small></td> |
- | <td class="xl1526463" align="right">1.98</td> | + | <td class="xl1526463" align="right"><small>1.98</small></td> |
- | <td class="xl1526463" align="right">18.92</td> | + | <td class="xl1526463" align="right"><small>18.92</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl1526463" style="height: 15pt;" | | <td class="xl1526463" style="height: 15pt;" |
- | height="20">T-W3</td> | + | height="20"><small>T-W3</small></td> |
- | <td class="xl1526463">FreiGEM</td> | + | <td class="xl1526463"><small>FreiGEM</small></td> |
- | <td class="xl6326463" align="right">20.07.2009</td> | + | <td class="xl6326463" align="right"><small>20.07.2009</small></td> |
- | <td class="xl6426463" align="right">14:21</td> | + | <td class="xl6426463" align="right"><small>14:21</small></td> |
- | <td class="xl1526463" align="right">1.65</td> | + | <td class="xl1526463" align="right"><small>1.65</small></td> |
- | <td class="xl1526463" align="right">4.22</td> | + | <td class="xl1526463" align="right"><small>4.22</small></td> |
- | <td class="xl1526463" align="right">1.65</td> | + | <td class="xl1526463" align="right"><small>1.65</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl1526463" style="height: 15pt;" | | <td class="xl1526463" style="height: 15pt;" |
- | height="20">T-W3</td> | + | height="20"><small>T-W3</small></td> |
- | <td class="xl1526463">FreiGEM</td> | + | <td class="xl1526463"><small>FreiGEM</small></td> |
- | <td class="xl6326463" align="right">20.07.2009</td> | + | <td class="xl6326463" align="right"><small>20.07.2009</small></td> |
- | <td class="xl6426463" align="right">14:21</td> | + | <td class="xl6426463" align="right"><small>14:21</small></td> |
- | <td class="xl1526463" align="right">7.34</td> | + | <td class="xl1526463" align="right"><small>7.34</small></td> |
- | <td class="xl1526463" align="right">1.86</td> | + | <td class="xl1526463" align="right"><small>1.86</small></td> |
- | <td class="xl1526463" align="right">7.337</td> | + | <td class="xl1526463" align="right"><small>7.337</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl1526463" style="height: 15pt;" | | <td class="xl1526463" style="height: 15pt;" |
- | height="20">T-W4</td> | + | height="20"><small>T-W4</small></td> |
- | <td class="xl1526463">FreiGEM</td> | + | <td class="xl1526463"><small>FreiGEM</small></td> |
- | <td class="xl6326463" align="right">20.07.2009</td> | + | <td class="xl6326463" align="right"><small>20.07.2009</small></td> |
- | <td class="xl6426463" align="right">14:22</td> | + | <td class="xl6426463" align="right"><small>14:22</small></td> |
- | <td class="xl1526463" align="right">-1.3</td> | + | <td class="xl1526463" align="right"><small>-1.3</small></td> |
- | <td class="xl1526463" align="right">1.69</td> | + | <td class="xl1526463" align="right"><small>1.69</small></td> |
- | <td class="xl1526463" align="right">-1.301</td> | + | <td class="xl1526463" align="right"><small>-1.301</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl1526463" style="height: 15pt;" | | <td class="xl1526463" style="height: 15pt;" |
- | height="20">T-W4</td> | + | height="20"><small>T-W4</small></td> |
- | <td class="xl1526463">FreiGEM</td> | + | <td class="xl1526463"><small>FreiGEM</small></td> |
- | <td class="xl6326463" align="right">20.07.2009</td> | + | <td class="xl6326463" align="right"><small>20.07.2009</small></td> |
- | <td class="xl6426463" align="right">14:23</td> | + | <td class="xl6426463" align="right"><small>14:23</small></td> |
- | <td class="xl1526463" align="right">5.36</td> | + | <td class="xl1526463" align="right"><small>5.36</small></td> |
- | <td class="xl1526463" align="right">1.67</td> | + | <td class="xl1526463" align="right"><small>1.67</small></td> |
- | <td class="xl1526463" align="right">5.36</td> | + | <td class="xl1526463" align="right"><small>5.36</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl1526463" style="height: 15pt;" | | <td class="xl1526463" style="height: 15pt;" |
- | height="20">T-W5</td> | + | height="20"><small>T-W5</small></td> |
- | <td class="xl1526463">FreiGEM</td> | + | <td class="xl1526463"><small>FreiGEM</small></td> |
- | <td class="xl6326463" align="right">20.07.2009</td> | + | <td class="xl6326463" align="right"><small>20.07.2009</small></td> |
- | <td class="xl6426463" align="right">14:23</td> | + | <td class="xl6426463" align="right"><small>14:23</small></td> |
- | <td class="xl1526463" align="right">3.54</td> | + | <td class="xl1526463" align="right"><small>3.54</small></td> |
- | <td class="xl1526463" align="right">1.74</td> | + | <td class="xl1526463" align="right"><small>1.74</small></td> |
- | <td class="xl1526463" align="right">3.543</td> | + | <td class="xl1526463" align="right"><small>3.543</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl1526463" style="height: 15pt;" | | <td class="xl1526463" style="height: 15pt;" |
- | height="20">A-W1</td> | + | height="20"><small>A-W1</small></td> |
- | <td class="xl1526463">FreiGEM</td> | + | <td class="xl1526463"><small>FreiGEM</small></td> |
- | <td class="xl6326463" align="right">20.07.2009</td> | + | <td class="xl6326463" align="right"><small>20.07.2009</small></td> |
- | <td class="xl6426463" align="right">14:25</td> | + | <td class="xl6426463" align="right"><small>14:25</small></td> |
- | <td class="xl1526463" align="right">26.8</td> | + | <td class="xl1526463" align="right"><small>26.8</small></td> |
- | <td class="xl1526463" align="right">1.94</td> | + | <td class="xl1526463" align="right"><small>1.94</small></td> |
- | <td class="xl1526463" align="right">26.802</td> | + | <td class="xl1526463" align="right"><small>26.802</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl1526463" style="height: 15pt;" | | <td class="xl1526463" style="height: 15pt;" |
- | height="20">A-W2</td> | + | height="20"><small>A-W2</small></td> |
- | <td class="xl1526463">FreiGEM</td> | + | <td class="xl1526463"><small>FreiGEM</small></td> |
- | <td class="xl6326463" align="right">20.07.2009</td> | + | <td class="xl6326463" align="right"><small>20.07.2009</small></td> |
- | <td class="xl6426463" align="right">14:25</td> | + | <td class="xl6426463" align="right"><small>14:25</small></td> |
- | <td class="xl1526463" align="right">18.62</td> | + | <td class="xl1526463" align="right"><small>18.62</small></td> |
- | <td class="xl1526463" align="right">1.93</td> | + | <td class="xl1526463" align="right"><small>1.93</small></td> |
- | <td class="xl1526463" align="right">18.621</td> | + | <td class="xl1526463" align="right"><small>18.621</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl1526463" style="height: 15pt;" | | <td class="xl1526463" style="height: 15pt;" |
- | height="20">A-W3</td> | + | height="20"><small>A-W3</small></td> |
- | <td class="xl1526463">FreiGEM</td> | + | <td class="xl1526463"><small>FreiGEM</small></td> |
- | <td class="xl6326463" align="right">20.07.2009</td> | + | <td class="xl6326463" align="right"><small>20.07.2009</small></td> |
- | <td class="xl6426463" align="right">14:27</td> | + | <td class="xl6426463" align="right"><small>14:27</small></td> |
- | <td class="xl1526463" align="right">6.94</td> | + | <td class="xl1526463" align="right"><small>6.94</small></td> |
- | <td class="xl1526463" align="right">1.78</td> | + | <td class="xl1526463" align="right"><small>1.78</small></td> |
- | <td class="xl1526463" align="right">6.938</td> | + | <td class="xl1526463" align="right"><small>6.938</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl1526463" style="height: 15pt;" | | <td class="xl1526463" style="height: 15pt;" |
- | height="20">A-W4</td> | + | height="20"><small>A-W4</small></td> |
- | <td class="xl1526463">FreiGEM</td> | + | <td class="xl1526463"><small>FreiGEM</small></td> |
- | <td class="xl6326463" align="right">20.07.2009</td> | + | <td class="xl6326463" align="right"><small>20.07.2009</small></td> |
- | <td class="xl6426463" align="right">14:28</td> | + | <td class="xl6426463" align="right"><small>14:28</small></td> |
- | <td class="xl1526463" align="right">5.35</td> | + | <td class="xl1526463" align="right"><small>5.35</small></td> |
- | <td class="xl1526463" align="right">1.56</td> | + | <td class="xl1526463" align="right"><small>1.56</small></td> |
- | <td class="xl1526463" align="right">5.351</td> | + | <td class="xl1526463" align="right"><small>5.351</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl1526463" style="height: 15pt;" | | <td class="xl1526463" style="height: 15pt;" |
- | height="20">A-W5</td> | + | height="20"><small>A-W5</small></td> |
- | <td class="xl1526463">FreiGEM</td> | + | <td class="xl1526463"><small>FreiGEM</small></td> |
- | <td class="xl6326463" align="right">20.07.2009</td> | + | <td class="xl6326463" align="right"><small>20.07.2009</small></td> |
- | <td class="xl6426463" align="right">14:28</td> | + | <td class="xl6426463" align="right"><small>14:28</small></td> |
- | <td class="xl1526463" align="right">2.96</td> | + | <td class="xl1526463" align="right"><small>2.96</small></td> |
- | <td class="xl1526463" align="right">1.6</td> | + | <td class="xl1526463" align="right"><small>1.6</small></td> |
- | <td class="xl1526463" align="right">2.959</td> | + | <td class="xl1526463" align="right"><small>2.959</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl1526463" style="height: 15pt;" | | <td class="xl1526463" style="height: 15pt;" |
- | height="20">A-W5</td> | + | height="20"><small>A-W5</small></td> |
- | <td class="xl1526463">FreiGEM</td> | + | <td class="xl1526463"><small>FreiGEM</small></td> |
- | <td class="xl6326463" align="right">20.07.2009</td> | + | <td class="xl6326463" align="right"><small>20.07.2009</small></td> |
- | <td class="xl6426463" align="right">14:29</td> | + | <td class="xl6426463" align="right"><small>14:29</small></td> |
- | <td class="xl1526463" align="right">2.88</td> | + | <td class="xl1526463" align="right"><small>2.88</small></td> |
- | <td class="xl1526463" align="right">1.62</td> | + | <td class="xl1526463" align="right"><small>1.62</small></td> |
- | <td class="xl1526463" align="right">2.881</td> | + | <td class="xl1526463" align="right"><small>2.881</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl1526463" style="height: 15pt;" | | <td class="xl1526463" style="height: 15pt;" |
- | height="20">T-E1</td> | + | height="20"><small>T-E1</small></td> |
- | <td class="xl1526463">FreiGEM</td> | + | <td class="xl1526463"><small>FreiGEM</small></td> |
- | <td class="xl6326463" align="right">20.07.2009</td> | + | <td class="xl6326463" align="right"><small>20.07.2009</small></td> |
- | <td class="xl6426463" align="right">14:30</td> | + | <td class="xl6426463" align="right"><small>14:30</small></td> |
- | <td class="xl1526463" align="right">2</td> | + | <td class="xl1526463" align="right"><small>2</small></td> |
- | <td class="xl1526463" align="right">1.72</td> | + | <td class="xl1526463" align="right"><small>1.72</small></td> |
- | <td class="xl1526463" align="right">2.002</td> | + | <td class="xl1526463" align="right"><small>2.002</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl1526463" style="height: 15pt;" | | <td class="xl1526463" style="height: 15pt;" |
- | height="20">T-E2</td> | + | height="20"><small>T-E2</small></td> |
- | <td class="xl1526463">FreiGEM</td> | + | <td class="xl1526463"><small>FreiGEM</small></td> |
- | <td class="xl6326463" align="right">20.07.2009</td> | + | <td class="xl6326463" align="right"><small>20.07.2009</small></td> |
- | <td class="xl6426463" align="right">14:31</td> | + | <td class="xl6426463" align="right"><small>14:31</small></td> |
- | <td class="xl1526463" align="right">0.22</td> | + | <td class="xl1526463" align="right"><small>0.22</small></td> |
- | <td class="xl1526463" align="right">2.58</td> | + | <td class="xl1526463" align="right"><small>2.58</small></td> |
- | <td class="xl1526463" align="right">0.219</td> | + | <td class="xl1526463" align="right"><small>0.219</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl1526463" style="height: 15pt;" | | <td class="xl1526463" style="height: 15pt;" |
- | height="20">T-E2</td> | + | height="20"><small>T-E2</small></td> |
- | <td class="xl1526463">FreiGEM</td> | + | <td class="xl1526463"><small>FreiGEM</small></td> |
- | <td class="xl6326463" align="right">20.07.2009</td> | + | <td class="xl6326463" align="right"><small>20.07.2009</small></td> |
- | <td class="xl6426463" align="right">14:32</td> | + | <td class="xl6426463" align="right"><small>14:32</small></td> |
- | <td class="xl1526463" align="right">1.04</td> | + | <td class="xl1526463" align="right"><small>1.04</small></td> |
- | <td class="xl1526463" align="right">1.89</td> | + | <td class="xl1526463" align="right"><small>1.89</small></td> |
- | <td class="xl1526463" align="right">1.042</td> | + | <td class="xl1526463" align="right"><small>1.042</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl1526463" style="height: 15pt;" | | <td class="xl1526463" style="height: 15pt;" |
- | height="20">T-E2</td> | + | height="20"><small>T-E2</small></td> |
- | <td class="xl1526463">FreiGEM</td> | + | <td class="xl1526463"><small>FreiGEM</small></td> |
- | <td class="xl6326463" align="right">20.07.2009</td> | + | <td class="xl6326463" align="right"><small>20.07.2009</small></td> |
- | <td class="xl6426463" align="right">14:32</td> | + | <td class="xl6426463" align="right"><small>14:32</small></td> |
- | <td class="xl1526463" align="right">0.73</td> | + | <td class="xl1526463" align="right"><small>0.73</small></td> |
- | <td class="xl1526463" align="right">1.7</td> | + | <td class="xl1526463" align="right"><small>1.7</small></td> |
- | <td class="xl1526463" align="right">0.735</td> | + | <td class="xl1526463" align="right"><small>0.735</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl1526463" style="height: 15pt;" | | <td class="xl1526463" style="height: 15pt;" |
- | height="20">T-E2</td> | + | height="20"><small>T-E2</small></td> |
- | <td class="xl1526463">FreiGEM</td> | + | <td class="xl1526463"><small>FreiGEM</small></td> |
- | <td class="xl6326463" align="right">20.07.2009</td> | + | <td class="xl6326463" align="right"><small>20.07.2009</small></td> |
- | <td class="xl6426463" align="right">14:33</td> | + | <td class="xl6426463" align="right"><small>14:33</small></td> |
- | <td class="xl1526463" align="right">0.54</td> | + | <td class="xl1526463" align="right"><small>0.54</small></td> |
- | <td class="xl1526463" align="right">2.02</td> | + | <td class="xl1526463" align="right"><small>2.02</small></td> |
- | <td class="xl1526463" align="right">0.536</td> | + | <td class="xl1526463" align="right"><small>0.536</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl1526463" style="height: 15pt;" | | <td class="xl1526463" style="height: 15pt;" |
- | height="20">T-E2</td> | + | height="20"><small>T-E2</small></td> |
- | <td class="xl1526463">FreiGEM</td> | + | <td class="xl1526463"><small>FreiGEM</small></td> |
- | <td class="xl6326463" align="right">20.07.2009</td> | + | <td class="xl6326463" align="right"><small>20.07.2009</small></td> |
- | <td class="xl6426463" align="right">14:34</td> | + | <td class="xl6426463" align="right"><small>14:34</small></td> |
- | <td class="xl1526463" align="right">1.44</td> | + | <td class="xl1526463" align="right"><small>1.44</small></td> |
- | <td class="xl1526463" align="right">1.73</td> | + | <td class="xl1526463" align="right"><small>1.73</small></td> |
- | <td class="xl1526463" align="right">1.438</td> | + | <td class="xl1526463" align="right"><small>1.438</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl1526463" style="height: 15pt;" | | <td class="xl1526463" style="height: 15pt;" |
- | height="20">T-E3</td> | + | height="20"><small>T-E3</small></td> |
- | <td class="xl1526463">FreiGEM</td> | + | <td class="xl1526463"><small>FreiGEM</small></td> |
- | <td class="xl6326463" align="right">20.07.2009</td> | + | <td class="xl6326463" align="right"><small>20.07.2009</small></td> |
- | <td class="xl6426463" align="right">14:34</td> | + | <td class="xl6426463" align="right"><small>14:34</small></td> |
- | <td class="xl1526463" align="right">2.19</td> | + | <td class="xl1526463" align="right"><small>2.19</small></td> |
- | <td class="xl1526463" align="right">1.38</td> | + | <td class="xl1526463" align="right"><small>1.38</small></td> |
- | <td class="xl1526463" align="right">2.194</td> | + | <td class="xl1526463" align="right"><small>2.194</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl1526463" style="height: 15pt;" | | <td class="xl1526463" style="height: 15pt;" |
- | height="20">T-E4</td> | + | height="20"><small>T-E4</small></td> |
- | <td class="xl1526463">FreiGEM</td> | + | <td class="xl1526463"><small>FreiGEM</small></td> |
- | <td class="xl6326463" align="right">20.07.2009</td> | + | <td class="xl6326463" align="right"><small>20.07.2009</small></td> |
- | <td class="xl6426463" align="right">14:35</td> | + | <td class="xl6426463" align="right"><small>14:35</small></td> |
- | <td class="xl1526463" align="right">2.08</td> | + | <td class="xl1526463" align="right"><small>2.08</small></td> |
- | <td class="xl1526463" align="right">1.12</td> | + | <td class="xl1526463" align="right"><small>1.12</small></td> |
- | <td class="xl1526463" align="right">2.078</td> | + | <td class="xl1526463" align="right"><small>2.078</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl1526463" style="height: 15pt;" | | <td class="xl1526463" style="height: 15pt;" |
- | height="20">T-E5</td> | + | height="20"><small>T-E5</small></td> |
- | <td class="xl1526463">FreiGEM</td> | + | <td class="xl1526463"><small>FreiGEM</small></td> |
- | <td class="xl6326463" align="right">20.07.2009</td> | + | <td class="xl6326463" align="right"><small>20.07.2009</small></td> |
- | <td class="xl6426463" align="right">14:35</td> | + | <td class="xl6426463" align="right"><small>14:35</small></td> |
- | <td class="xl1526463" align="right">0.68</td> | + | <td class="xl1526463" align="right"><small>0.68</small></td> |
- | <td class="xl1526463" align="right">1.42</td> | + | <td class="xl1526463" align="right"><small>1.42</small></td> |
- | <td class="xl1526463" align="right">0.679</td> | + | <td class="xl1526463" align="right"><small>0.679</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl1526463" style="height: 15pt;" | | <td class="xl1526463" style="height: 15pt;" |
- | height="20">T-E6</td> | + | height="20"><small>T-E6</small></td> |
- | <td class="xl1526463">FreiGEM</td> | + | <td class="xl1526463"><small>FreiGEM</small></td> |
- | <td class="xl6326463" align="right">20.07.2009</td> | + | <td class="xl6326463" align="right"><small>20.07.2009</small></td> |
- | <td class="xl6426463" align="right">14:36</td> | + | <td class="xl6426463" align="right"><small>14:36</small></td> |
- | <td class="xl1526463" align="right">0.24</td> | + | <td class="xl1526463" align="right"><small>0.24</small></td> |
- | <td class="xl1526463" align="right">1.68</td> | + | <td class="xl1526463" align="right"><small>1.68</small></td> |
- | <td class="xl1526463" align="right">0.237</td> | + | <td class="xl1526463" align="right"><small>0.237</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl1526463" style="height: 15pt;" | | <td class="xl1526463" style="height: 15pt;" |
- | height="20">T-E7</td> | + | height="20"><small>T-E7</small></td> |
- | <td class="xl1526463">FreiGEM</td> | + | <td class="xl1526463"><small>FreiGEM</small></td> |
- | <td class="xl6326463" align="right">20.07.2009</td> | + | <td class="xl6326463" align="right"><small>20.07.2009</small></td> |
- | <td class="xl6426463" align="right">14:36</td> | + | <td class="xl6426463" align="right"><small>14:36</small></td> |
- | <td class="xl1526463" align="right">0.3</td> | + | <td class="xl1526463" align="right"><small>0.3</small></td> |
- | <td class="xl1526463" align="right">1.59</td> | + | <td class="xl1526463" align="right"><small>1.59</small></td> |
- | <td class="xl1526463" align="right">0.303</td> | + | <td class="xl1526463" align="right"><small>0.303</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl1526463" style="height: 15pt;" | | <td class="xl1526463" style="height: 15pt;" |
- | height="20">T-E8</td> | + | height="20"><small>T-E8</small></td> |
- | <td class="xl1526463">FreiGEM</td> | + | <td class="xl1526463"><small>FreiGEM</small></td> |
- | <td class="xl6326463" align="right">20.07.2009</td> | + | <td class="xl6326463" align="right"><small>20.07.2009</small></td> |
- | <td class="xl6426463" align="right">14:37</td> | + | <td class="xl6426463" align="right"><small>14:37</small></td> |
- | <td class="xl1526463" align="right">0.31</td> | + | <td class="xl1526463" align="right"><small>0.31</small></td> |
- | <td class="xl1526463" align="right">1.38</td> | + | <td class="xl1526463" align="right"><small>1.38</small></td> |
- | <td class="xl1526463" align="right">0.308</td> | + | <td class="xl1526463" align="right"><small>0.308</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl1526463" style="height: 15pt;" | | <td class="xl1526463" style="height: 15pt;" |
- | height="20">T-E9</td> | + | height="20"><small>T-E9</small></td> |
- | <td class="xl1526463">FreiGEM</td> | + | <td class="xl1526463"><small>FreiGEM</small></td> |
- | <td class="xl6326463" align="right">20.07.2009</td> | + | <td class="xl6326463" align="right"><small>20.07.2009</small></td> |
- | <td class="xl6426463" align="right">14:37</td> | + | <td class="xl6426463" align="right"><small>14:37</small></td> |
- | <td class="xl1526463" align="right">0.23</td> | + | <td class="xl1526463" align="right"><small>0.23</small></td> |
- | <td class="xl1526463" align="right">1.43</td> | + | <td class="xl1526463" align="right"><small>1.43</small></td> |
- | <td class="xl1526463" align="right">0.231</td> | + | <td class="xl1526463" align="right"><small>0.231</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl1526463" style="height: 15pt;" | | <td class="xl1526463" style="height: 15pt;" |
- | height="20">T-E10</td> | + | height="20"><small>T-E10</small></td> |
- | <td class="xl1526463">FreiGEM</td> | + | <td class="xl1526463"><small>FreiGEM</small></td> |
- | <td class="xl6326463" align="right">20.07.2009</td> | + | <td class="xl6326463" align="right"><small>20.07.2009</small></td> |
- | <td class="xl6426463" align="right">14:38</td> | + | <td class="xl6426463" align="right"><small>14:38</small></td> |
- | <td class="xl1526463" align="right">0.21</td> | + | <td class="xl1526463" align="right"><small>0.21</small></td> |
- | <td class="xl1526463" align="right">1.34</td> | + | <td class="xl1526463" align="right"><small>1.34</small></td> |
- | <td class="xl1526463" align="right">0.211</td> | + | <td class="xl1526463" align="right"><small>0.211</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl1526463" style="height: 15pt;" | | <td class="xl1526463" style="height: 15pt;" |
- | height="20">A-E1</td> | + | height="20"><small>A-E1</small></td> |
- | <td class="xl1526463">FreiGEM</td> | + | <td class="xl1526463"><small>FreiGEM</small></td> |
- | <td class="xl6326463" align="right">20.07.2009</td> | + | <td class="xl6326463" align="right"><small>20.07.2009</small></td> |
- | <td class="xl6426463" align="right">14:39</td> | + | <td class="xl6426463" align="right"><small>14:39</small></td> |
- | <td class="xl1526463" align="right">0.92</td> | + | <td class="xl1526463" align="right"><small>0.92</small></td> |
- | <td class="xl1526463" align="right">1.76</td> | + | <td class="xl1526463" align="right"><small>1.76</small></td> |
- | <td class="xl1526463" align="right">0.925</td> | + | <td class="xl1526463" align="right"><small>0.925</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl1526463" style="height: 15pt;" | | <td class="xl1526463" style="height: 15pt;" |
- | height="20">A-E1</td> | + | height="20"><small>A-E1</small></td> |
- | <td class="xl1526463">FreiGEM</td> | + | <td class="xl1526463"><small>FreiGEM</small></td> |
- | <td class="xl6326463" align="right">20.07.2009</td> | + | <td class="xl6326463" align="right"><small>20.07.2009</small></td> |
- | <td class="xl6426463" align="right">14:40</td> | + | <td class="xl6426463" align="right"><small>14:40</small></td> |
- | <td class="xl1526463" align="right">1.95</td> | + | <td class="xl1526463" align="right"><small>1.95</small></td> |
- | <td class="xl1526463" align="right">1.7</td> | + | <td class="xl1526463" align="right"><small>1.7</small></td> |
- | <td class="xl1526463" align="right">1.945</td> | + | <td class="xl1526463" align="right"><small>1.945</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl1526463" style="height: 15pt;" | | <td class="xl1526463" style="height: 15pt;" |
- | height="20">A-E2</td> | + | height="20"><small>A-E2</small></td> |
- | <td class="xl1526463">FreiGEM</td> | + | <td class="xl1526463"><small>FreiGEM</small></td> |
- | <td class="xl6326463" align="right">20.07.2009</td> | + | <td class="xl6326463" align="right"><small>20.07.2009</small></td> |
- | <td class="xl6426463" align="right">14:41</td> | + | <td class="xl6426463" align="right"><small>14:41</small></td> |
- | <td class="xl1526463" align="right">0.41</td> | + | <td class="xl1526463" align="right"><small>0.41</small></td> |
- | <td class="xl1526463" align="right">1.89</td> | + | <td class="xl1526463" align="right"><small>1.89</small></td> |
- | <td class="xl1526463" align="right">0.409</td> | + | <td class="xl1526463" align="right"><small>0.409</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl1526463" style="height: 15pt;" | | <td class="xl1526463" style="height: 15pt;" |
- | height="20">A-E2</td> | + | height="20"><small>A-E2</small></td> |
- | <td class="xl1526463">FreiGEM</td> | + | <td class="xl1526463"><small>FreiGEM</small></td> |
- | <td class="xl6326463" align="right">20.07.2009</td> | + | <td class="xl6326463" align="right"><small>20.07.2009</small></td> |
- | <td class="xl6426463" align="right">14:41</td> | + | <td class="xl6426463" align="right"><small>14:41</small></td> |
- | <td class="xl1526463" align="right">1.31</td> | + | <td class="xl1526463" align="right"><small>1.31</small></td> |
- | <td class="xl1526463" align="right">1.71</td> | + | <td class="xl1526463" align="right"><small>1.71</small></td> |
- | <td class="xl1526463" align="right">1.31</td> | + | <td class="xl1526463" align="right"><small>1.31</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl1526463" style="height: 15pt;" | | <td class="xl1526463" style="height: 15pt;" |
- | height="20">A-E3</td> | + | height="20"><small>A-E3</small></td> |
- | <td class="xl1526463">FreiGEM</td> | + | <td class="xl1526463"><small>FreiGEM</small></td> |
- | <td class="xl6326463" align="right">20.07.2009</td> | + | <td class="xl6326463" align="right"><small>20.07.2009</small></td> |
- | <td class="xl6426463" align="right">14:42</td> | + | <td class="xl6426463" align="right"><small>14:42</small></td> |
- | <td class="xl1526463" align="right">1.89</td> | + | <td class="xl1526463" align="right"><small>1.89</small></td> |
- | <td class="xl1526463" align="right">1.49</td> | + | <td class="xl1526463" align="right"><small>1.49</small></td> |
- | <td class="xl1526463" align="right">1.892</td> | + | <td class="xl1526463" align="right"><small>1.892</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl1526463" style="height: 15pt;" | | <td class="xl1526463" style="height: 15pt;" |
- | height="20">A-E4</td> | + | height="20"><small>A-E4</small></td> |
- | <td class="xl1526463">FreiGEM</td> | + | <td class="xl1526463"><small>FreiGEM</small></td> |
- | <td class="xl6326463" align="right">20.07.2009</td> | + | <td class="xl6326463" align="right"><small>20.07.2009</small></td> |
- | <td class="xl6426463" align="right">14:42</td> | + | <td class="xl6426463" align="right"><small>14:42</small></td> |
- | <td class="xl1526463" align="right">4.43</td> | + | <td class="xl1526463" align="right"><small>4.43</small></td> |
- | <td class="xl1526463" align="right">0.84</td> | + | <td class="xl1526463" align="right"><small>0.84</small></td> |
- | <td class="xl1526463" align="right">4.428</td> | + | <td class="xl1526463" align="right"><small>4.428</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl1526463" style="height: 15pt;" | | <td class="xl1526463" style="height: 15pt;" |
- | height="20">A-E5</td> | + | height="20"><small>A-E5</small></td> |
- | <td class="xl1526463">FreiGEM</td> | + | <td class="xl1526463"><small>FreiGEM</small></td> |
- | <td class="xl6326463" align="right">20.07.2009</td> | + | <td class="xl6326463" align="right"><small>20.07.2009</small></td> |
- | <td class="xl6426463" align="right">14:43</td> | + | <td class="xl6426463" align="right"><small>14:43</small></td> |
- | <td class="xl1526463" align="right">1.06</td> | + | <td class="xl1526463" align="right"><small>1.06</small></td> |
- | <td class="xl1526463" align="right">1.23</td> | + | <td class="xl1526463" align="right"><small>1.23</small></td> |
- | <td class="xl1526463" align="right">1.06</td> | + | <td class="xl1526463" align="right"><small>1.06</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl1526463" style="height: 15pt;" | | <td class="xl1526463" style="height: 15pt;" |
- | height="20">A-E6</td> | + | height="20"><small>A-E6</small></td> |
- | <td class="xl1526463">FreiGEM</td> | + | <td class="xl1526463"><small>FreiGEM</small></td> |
- | <td class="xl6326463" align="right">20.07.2009</td> | + | <td class="xl6326463" align="right"><small>20.07.2009</small></td> |
- | <td class="xl6426463" align="right">14:44</td> | + | <td class="xl6426463" align="right"><small>14:44</small></td> |
- | <td class="xl1526463" align="right">0.46</td> | + | <td class="xl1526463" align="right"><small>0.46</small></td> |
- | <td class="xl1526463" align="right">1.35</td> | + | <td class="xl1526463" align="right"><small>1.35</small></td> |
- | <td class="xl1526463" align="right">0.46</td> | + | <td class="xl1526463" align="right"><small>0.46</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl1526463" style="height: 15pt;" | | <td class="xl1526463" style="height: 15pt;" |
- | height="20">A-E7</td> | + | height="20"><small>A-E7</small></td> |
- | <td class="xl1526463">FreiGEM</td> | + | <td class="xl1526463"><small>FreiGEM</small></td> |
- | <td class="xl6326463" align="right">20.07.2009</td> | + | <td class="xl6326463" align="right"><small>20.07.2009</small></td> |
- | <td class="xl6426463" align="right">14:44</td> | + | <td class="xl6426463" align="right"><small>14:44</small></td> |
- | <td class="xl1526463" align="right">0.12</td> | + | <td class="xl1526463" align="right"><small>0.12</small></td> |
- | <td class="xl1526463" align="right">2.02</td> | + | <td class="xl1526463" align="right"><small>2.02</small></td> |
- | <td class="xl1526463" align="right">0.115</td> | + | <td class="xl1526463" align="right"><small>0.115</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl1526463" style="height: 15pt;" | | <td class="xl1526463" style="height: 15pt;" |
- | height="20">A-E8</td> | + | height="20"><small>A-E8</small></td> |
- | <td class="xl1526463">FreiGEM</td> | + | <td class="xl1526463"><small>FreiGEM</small></td> |
- | <td class="xl6326463" align="right">20.07.2009</td> | + | <td class="xl6326463" align="right"><small>20.07.2009</small></td> |
- | <td class="xl6426463" align="right">14:44</td> | + | <td class="xl6426463" align="right"><small>14:44</small></td> |
- | <td class="xl1526463" align="right">0.27</td> | + | <td class="xl1526463" align="right"><small>0.27</small></td> |
- | <td class="xl1526463" align="right">1.5</td> | + | <td class="xl1526463" align="right"><small>1.5</small></td> |
- | <td class="xl1526463" align="right">0.272</td> | + | <td class="xl1526463" align="right"><small>0.272</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl1526463" style="height: 15pt;" | | <td class="xl1526463" style="height: 15pt;" |
- | height="20">A-E9</td> | + | height="20"><small>A-E9</small></td> |
- | <td class="xl1526463">FreiGEM</td> | + | <td class="xl1526463"><small>FreiGEM</small></td> |
- | <td class="xl6326463" align="right">20.07.2009</td> | + | <td class="xl6326463" align="right"><small>20.07.2009</small></td> |
- | <td class="xl6426463" align="right">14:45</td> | + | <td class="xl6426463" align="right"><small>14:45</small></td> |
- | <td class="xl1526463" align="right">0.28</td> | + | <td class="xl1526463" align="right"><small>0.28</small></td> |
- | <td class="xl1526463" align="right">1.49</td> | + | <td class="xl1526463" align="right"><small>1.49</small></td> |
- | <td class="xl1526463" align="right">0.281</td> | + | <td class="xl1526463" align="right"><small>0.281</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl1526463" style="height: 15pt;" | | <td class="xl1526463" style="height: 15pt;" |
- | height="20">A-E10</td> | + | height="20"><small>A-E10</small></td> |
- | <td class="xl1526463">FreiGEM</td> | + | <td class="xl1526463"><small>FreiGEM</small></td> |
- | <td class="xl6326463" align="right">20.07.2009</td> | + | <td class="xl6326463" align="right"><small>20.07.2009</small></td> |
- | <td class="xl6426463" align="right">14:45</td> | + | <td class="xl6426463" align="right"><small>14:45</small></td> |
- | <td class="xl1526463" align="right">0.27</td> | + | <td class="xl1526463" align="right"><small>0.27</small></td> |
- | <td class="xl1526463" align="right">1.4</td> | + | <td class="xl1526463" align="right"><small>1.4</small></td> |
- | <td class="xl1526463" align="right">0.274</td> | + | <td class="xl1526463" align="right"><small>0.274</small></td> |
| </tr> | | </tr> |
| <tr style="display: none;" height="0"> | | <tr style="display: none;" height="0"> |
Line 2,565: |
Line 2,490: |
| <br /> | | <br /> |
| <ul> | | <ul> |
- | <li> made TBS buffer (100ml) | + | <li> made TBS buffer (100ml) </li> |
- | </li>
| + | |
| <li> start preparation of M13 phage ssDNA :overnight | | <li> start preparation of M13 phage ssDNA :overnight |
- | breeding of ER2738 | + | breeding of ER2738 </li> |
- | </li>
| + | |
| </ul> | | </ul> |
| <h3>21.07.09, Isabel, Sarah, Manuel, Max</h3> | | <h3>21.07.09, Isabel, Sarah, Manuel, Max</h3> |
| <br /> | | <br /> |
| <ul> | | <ul> |
- | <li> made a glycerol stock of ER2738 Tet | + | <li> made a glycerol stock of ER2738 Tet </li> |
- | </li>
| + | |
| <li> transformation of ER2738 with M13 phage, breeding | | <li> transformation of ER2738 with M13 phage, breeding |
- | and start phage precipitation | + | and start phage precipitation </li> |
- | </li>
| + | |
| <li>inoculation of the ligation Fok (inactive)+Split-Linker(2 | | <li>inoculation of the ligation Fok (inactive)+Split-Linker(2 |
- | colonies picked of each) | + | colonies picked of each) </li> |
- | </li>
| + | <li>inoculation of Dig and Lipo (2 colonies picked of each) </li> |
- | <li>inoculation of Dig and Lipo (2 colonies picked of each) | + | |
- | </li>
| + | |
| <li>inoculation of Fok (active and inactive), Ligation neu 1 | | <li>inoculation of Fok (active and inactive), Ligation neu 1 |
- | and 2 | + | and 2 </li> |
- | </li>
| + | <li>lb-amp plates casted </li> |
- | <li>lb-amp plates casted | + | |
- | </li>
| + | |
| <li>transformation from yesterday (20.07.) plated on lb-amp | | <li>transformation from yesterday (20.07.) plated on lb-amp |
- | again: 100 µl and centrifuged residue | + | again: 100 µl and centrifuged residue </li> |
- | </li>
| + | |
| </ul> | | </ul> |
| <h3>21.07.09, Laura</h3> | | <h3>21.07.09, Laura</h3> |
Line 2,599: |
Line 2,515: |
| of primers and expressionvector (cloning strategies of genIII and the | | of primers and expressionvector (cloning strategies of genIII and the |
| order of the ligation of the Fok, linker etc has to follow) in the new | | order of the ligation of the Fok, linker etc has to follow) in the new |
- | folder: cloning strategies | + | folder: cloning strategies </li> |
- | </li>
| + | |
| </ul> | | </ul> |
| <h3>22.07.09, Isabel, Sarah</h3> | | <h3>22.07.09, Isabel, Sarah</h3> |
| <ul> | | <ul> |
- | <li> precipitation of phage M13 particles | + | <li> precipitation of phage M13 particles </li> |
- | </li>
| + | |
| </ul> | | </ul> |
| <h3>22.07.09, Laura, Christoph</h3> | | <h3>22.07.09, Laura, Christoph</h3> |
Line 2,612: |
Line 2,526: |
| <li>plasmidpreparation of Ligation (pMAL&CAT) neu 1 | | <li>plasmidpreparation of Ligation (pMAL&CAT) neu 1 |
| & 2, Split linker, Digoxigenin 1 & 2, Lipocalin 1 | | & 2, Split linker, Digoxigenin 1 & 2, Lipocalin 1 |
- | & 2, Fok/Split Linker 1 & 2 | + | & 2, Fok/Split Linker 1 & 2 </li> |
- | </li>
| + | |
| <li>Glycerolstocks of Ligation neu 1 & 2 (Sven did | | <li>Glycerolstocks of Ligation neu 1 & 2 (Sven did |
- | stocks of Foka & Foki, don't know where he put it) | + | stocks of Foka & Foki, don't know where he put it) </li> |
- | </li>
| + | <li>digest of His, Strep, Digoxigenin, Lipocalin </li> |
- | <li>digest of His, Strep, Digoxigenin, Lipocalin | + | <li>agarose gel of digests </li> |
- | </li>
| + | |
- | <li>agarose gel of digests | + | |
- | </li>
| + | |
| <li>gelextraction of cutted bands -> low concetrations </li> | | <li>gelextraction of cutted bands -> low concetrations </li> |
| <li>ligation with T4 Ligase of His/Dig, His/Lipo, Strep/Dig, | | <li>ligation with T4 Ligase of His/Dig, His/Lipo, Strep/Dig, |
- | Strep/Lipo -> put it in waterbath for overnight ligation | + | Strep/Lipo -> put it in waterbath for overnight ligation </li> |
- | </li>
| + | |
| <li><b>Nanodrop data</b></li> | | <li><b>Nanodrop data</b></li> |
| </ul> | | </ul> |
Line 2,634: |
Line 2,543: |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl657169" style="height: 15pt; width: 107pt;" | | <td class="xl657169" style="height: 15pt; width: 107pt;" |
- | height="20" width="143">Sample | + | height="20" width="143"><small>Sample |
- | ID</td> | + | ID</small></td> |
| <td class="xl657169" style="width: 48pt;" | | <td class="xl657169" style="width: 48pt;" |
- | width="64">User ID</td> | + | width="64"><small>User ID</small></td> |
| <td class="xl657169" style="width: 53pt;" | | <td class="xl657169" style="width: 53pt;" |
- | width="71">Date<span style=""> </span></td> | + | width="71"><small>Date<span style=""> </span></small></td> |
| <td class="xl657169" style="width: 48pt;" | | <td class="xl657169" style="width: 48pt;" |
- | width="64">Time<span style=""> </span></td> | + | width="64"><small>Time<span style=""> </span></small></td> |
| <td class="xl657169" style="width: 48pt;" | | <td class="xl657169" style="width: 48pt;" |
- | width="64">ng/ul<span style=""> </span></td> | + | width="64"><small>ng/ul<span style=""> </span></small></td> |
| <td class="xl657169" style="width: 48pt;" | | <td class="xl657169" style="width: 48pt;" |
- | width="64">A260<span style=""> </span></td> | + | width="64"><small>A260<span style=""> </span></small></td> |
| <td class="xl657169" style="width: 66pt;" | | <td class="xl657169" style="width: 66pt;" |
- | width="88">A280<span style=""> </span></td> | + | width="88"><small>A280<span style=""> </span></small></td> |
| <td class="xl657169" style="width: 71pt;" | | <td class="xl657169" style="width: 71pt;" |
- | width="94">260/280<span style=""> </span></td> | + | width="94"><small>260/280<span style=""> </span></small></td> |
| <td class="xl657169" style="width: 95pt;" | | <td class="xl657169" style="width: 95pt;" |
- | width="126">260/230<span style=""> </span></td> | + | width="126"><small>260/230<span style=""> |
| + | </span></small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl157169" style="height: 15pt;" | | <td class="xl157169" style="height: 15pt;" |
- | height="20">lig neu 1</td> | + | height="20"><small>lig neu 1</small></td> |
- | <td class="xl157169">FreiGEM</td> | + | <td class="xl157169"><small>FreiGEM</small></td> |
- | <td class="xl637169" align="right">22.07.2009</td> | + | <td class="xl637169" align="right"><small>22.07.2009</small></td> |
- | <td class="xl647169" align="right">11:44</td> | + | <td class="xl647169" align="right"><small>11:44</small></td> |
- | <td class="xl157169" align="right">44.77</td> | + | <td class="xl157169" align="right"><small>44.77</small></td> |
- | <td class="xl157169" align="right">0.895</td> | + | <td class="xl157169" align="right"><small>0.895</small></td> |
- | <td class="xl157169" align="right">0.477</td> | + | <td class="xl157169" align="right"><small>0.477</small></td> |
- | <td class="xl157169" align="right">1.88</td> | + | <td class="xl157169" align="right"><small>1.88</small></td> |
- | <td class="xl157169" align="right">2.2</td> | + | <td class="xl157169" align="right"><small>2.2</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl157169" style="height: 15pt;" | | <td class="xl157169" style="height: 15pt;" |
- | height="20">lig neu 2</td> | + | height="20"><small>lig neu 2</small></td> |
- | <td class="xl157169">FreiGEM</td> | + | <td class="xl157169"><small>FreiGEM</small></td> |
- | <td class="xl637169" align="right">22.07.2009</td> | + | <td class="xl637169" align="right"><small>22.07.2009</small></td> |
- | <td class="xl647169" align="right">11:46</td> | + | <td class="xl647169" align="right"><small>11:46</small></td> |
- | <td class="xl157169" align="right">40.9</td> | + | <td class="xl157169" align="right"><small>40.9</small></td> |
- | <td class="xl157169" align="right">0.818</td> | + | <td class="xl157169" align="right"><small>0.818</small></td> |
- | <td class="xl157169" align="right">0.413</td> | + | <td class="xl157169" align="right"><small>0.413</small></td> |
- | <td class="xl157169" align="right">1.98</td> | + | <td class="xl157169" align="right"><small>1.98</small></td> |
- | <td class="xl157169" align="right">1.99</td> | + | <td class="xl157169" align="right"><small>1.99</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl157169" style="height: 15pt;" | | <td class="xl157169" style="height: 15pt;" |
- | height="20">split linker</td> | + | height="20"><small>split linker</small></td> |
- | <td class="xl157169">FreiGEM</td> | + | <td class="xl157169"><small>FreiGEM</small></td> |
- | <td class="xl637169" align="right">22.07.2009</td> | + | <td class="xl637169" align="right"><small>22.07.2009</small></td> |
- | <td class="xl647169" align="right">11:47</td> | + | <td class="xl647169" align="right"><small>11:47</small></td> |
- | <td class="xl157169" align="right">246.09</td> | + | <td class="xl157169" align="right"><small>246.09</small></td> |
- | <td class="xl157169" align="right">4.922</td> | + | <td class="xl157169" align="right"><small>4.922</small></td> |
- | <td class="xl157169" align="right">2.631</td> | + | <td class="xl157169" align="right"><small>2.631</small></td> |
- | <td class="xl157169" align="right">1.87</td> | + | <td class="xl157169" align="right"><small>1.87</small></td> |
- | <td class="xl157169" align="right">1.84</td> | + | <td class="xl157169" align="right"><small>1.84</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl157169" style="height: 15pt;" | | <td class="xl157169" style="height: 15pt;" |
- | height="20">split linker</td> | + | height="20"><small>split linker</small></td> |
- | <td class="xl157169">FreiGEM</td> | + | <td class="xl157169"><small>FreiGEM</small></td> |
- | <td class="xl637169" align="right">22.07.2009</td> | + | <td class="xl637169" align="right"><small>22.07.2009</small></td> |
- | <td class="xl647169" align="right">11:47</td> | + | <td class="xl647169" align="right"><small>11:47</small></td> |
- | <td class="xl157169" align="right">245.47</td> | + | <td class="xl157169" align="right"><small>245.47</small></td> |
- | <td class="xl157169" align="right">4.909</td> | + | <td class="xl157169" align="right"><small>4.909</small></td> |
- | <td class="xl157169" align="right">2.606</td> | + | <td class="xl157169" align="right"><small>2.606</small></td> |
- | <td class="xl157169" align="right">1.88</td> | + | <td class="xl157169" align="right"><small>1.88</small></td> |
- | <td class="xl157169" align="right">1.9</td> | + | <td class="xl157169" align="right"><small>1.9</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl157169" style="height: 15pt;" | | <td class="xl157169" style="height: 15pt;" |
- | height="20">split linker</td> | + | height="20"><small>split linker</small></td> |
- | <td class="xl157169">FreiGEM</td> | + | <td class="xl157169"><small>FreiGEM</small></td> |
- | <td class="xl637169" align="right">22.07.2009</td> | + | <td class="xl637169" align="right"><small>22.07.2009</small></td> |
- | <td class="xl647169" align="right">11:48</td> | + | <td class="xl647169" align="right"><small>11:48</small></td> |
- | <td class="xl157169" align="right">261.74</td> | + | <td class="xl157169" align="right"><small>261.74</small></td> |
- | <td class="xl157169" align="right">5.235</td> | + | <td class="xl157169" align="right"><small>5.235</small></td> |
- | <td class="xl157169" align="right">2.825</td> | + | <td class="xl157169" align="right"><small>2.825</small></td> |
- | <td class="xl157169" align="right">1.85</td> | + | <td class="xl157169" align="right"><small>1.85</small></td> |
- | <td class="xl157169" align="right">1.66</td> | + | <td class="xl157169" align="right"><small>1.66</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl157169" style="height: 15pt;" | | <td class="xl157169" style="height: 15pt;" |
- | height="20">dig 1</td> | + | height="20"><small>dig 1</small></td> |
- | <td class="xl157169">FreiGEM</td> | + | <td class="xl157169"><small>FreiGEM</small></td> |
- | <td class="xl637169" align="right">22.07.2009</td> | + | <td class="xl637169" align="right"><small>22.07.2009</small></td> |
- | <td class="xl647169" align="right">11:49</td> | + | <td class="xl647169" align="right"><small>11:49</small></td> |
- | <td class="xl157169" align="right">337.99</td> | + | <td class="xl157169" align="right"><small>337.99</small></td> |
- | <td class="xl157169" align="right">6.76</td> | + | <td class="xl157169" align="right"><small>6.76</small></td> |
- | <td class="xl157169" align="right">3.572</td> | + | <td class="xl157169" align="right"><small>3.572</small></td> |
- | <td class="xl157169" align="right">1.89</td> | + | <td class="xl157169" align="right"><small>1.89</small></td> |
- | <td class="xl157169" align="right">1.99</td> | + | <td class="xl157169" align="right"><small>1.99</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl157169" style="height: 15pt;" | | <td class="xl157169" style="height: 15pt;" |
- | height="20">dig 2</td> | + | height="20"><small>dig 2</small></td> |
- | <td class="xl157169">FreiGEM</td> | + | <td class="xl157169"><small>FreiGEM</small></td> |
- | <td class="xl637169" align="right">22.07.2009</td> | + | <td class="xl637169" align="right"><small>22.07.2009</small></td> |
- | <td class="xl647169" align="right">11:50</td> | + | <td class="xl647169" align="right"><small>11:50</small></td> |
- | <td class="xl157169" align="right">313.46</td> | + | <td class="xl157169" align="right"><small>313.46</small></td> |
- | <td class="xl157169" align="right">6.269</td> | + | <td class="xl157169" align="right"><small>6.269</small></td> |
- | <td class="xl157169" align="right">3.308</td> | + | <td class="xl157169" align="right"><small>3.308</small></td> |
- | <td class="xl157169" align="right">1.9</td> | + | <td class="xl157169" align="right"><small>1.9</small></td> |
- | <td class="xl157169" align="right">2.21</td> | + | <td class="xl157169" align="right"><small>2.21</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl157169" style="height: 15pt;" | | <td class="xl157169" style="height: 15pt;" |
- | height="20">lip1</td> | + | height="20"><small>lip1</small></td> |
- | <td class="xl157169">FreiGEM</td> | + | <td class="xl157169"><small>FreiGEM</small></td> |
- | <td class="xl637169" align="right">22.07.2009</td> | + | <td class="xl637169" align="right"><small>22.07.2009</small></td> |
- | <td class="xl647169" align="right">11:51</td> | + | <td class="xl647169" align="right"><small>11:51</small></td> |
- | <td class="xl157169" align="right">269.33</td> | + | <td class="xl157169" align="right"><small>269.33</small></td> |
- | <td class="xl157169" align="right">5.387</td> | + | <td class="xl157169" align="right"><small>5.387</small></td> |
- | <td class="xl157169" align="right">2.832</td> | + | <td class="xl157169" align="right"><small>2.832</small></td> |
- | <td class="xl157169" align="right">1.9</td> | + | <td class="xl157169" align="right"><small>1.9</small></td> |
- | <td class="xl157169" align="right">1.9</td> | + | <td class="xl157169" align="right"><small>1.9</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl157169" style="height: 15pt;" | | <td class="xl157169" style="height: 15pt;" |
- | height="20">lip2</td> | + | height="20"><small>lip2</small></td> |
- | <td class="xl157169">FreiGEM</td> | + | <td class="xl157169"><small>FreiGEM</small></td> |
- | <td class="xl637169" align="right">22.07.2009</td> | + | <td class="xl637169" align="right"><small>22.07.2009</small></td> |
- | <td class="xl647169" align="right">11:52</td> | + | <td class="xl647169" align="right"><small>11:52</small></td> |
- | <td class="xl157169" align="right">295.78</td> | + | <td class="xl157169" align="right"><small>295.78</small></td> |
- | <td class="xl157169" align="right">5.916</td> | + | <td class="xl157169" align="right"><small>5.916</small></td> |
- | <td class="xl157169" align="right">3.122</td> | + | <td class="xl157169" align="right"><small>3.122</small></td> |
- | <td class="xl157169" align="right">1.9</td> | + | <td class="xl157169" align="right"><small>1.9</small></td> |
- | <td class="xl157169" align="right">2.1</td> | + | <td class="xl157169" align="right"><small>2.1</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl157169" style="height: 15pt;" | | <td class="xl157169" style="height: 15pt;" |
- | height="20">FOKi split 1</td> | + | height="20"><small>FOKi split 1</small></td> |
- | <td class="xl157169">FreiGEM</td> | + | <td class="xl157169"><small>FreiGEM</small></td> |
- | <td class="xl637169" align="right">22.07.2009</td> | + | <td class="xl637169" align="right"><small>22.07.2009</small></td> |
- | <td class="xl647169" align="right">11:53</td> | + | <td class="xl647169" align="right"><small>11:53</small></td> |
- | <td class="xl157169" align="right">257.42</td> | + | <td class="xl157169" align="right"><small>257.42</small></td> |
- | <td class="xl157169" align="right">5.148</td> | + | <td class="xl157169" align="right"><small>5.148</small></td> |
- | <td class="xl157169" align="right">2.678</td> | + | <td class="xl157169" align="right"><small>2.678</small></td> |
- | <td class="xl157169" align="right">1.92</td> | + | <td class="xl157169" align="right"><small>1.92</small></td> |
- | <td class="xl157169" align="right">2.24</td> | + | <td class="xl157169" align="right"><small>2.24</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl157169" style="height: 15pt;" | | <td class="xl157169" style="height: 15pt;" |
- | height="20">FOKi split 2</td> | + | height="20"><small>FOKi split 2</small></td> |
- | <td class="xl157169">FreiGEM</td> | + | <td class="xl157169"><small>FreiGEM</small></td> |
- | <td class="xl637169" align="right">22.07.2009</td> | + | <td class="xl637169" align="right"><small>22.07.2009</small></td> |
- | <td class="xl647169" align="right">11:54</td> | + | <td class="xl647169" align="right"><small>11:54</small></td> |
- | <td class="xl157169" align="right">237.28</td> | + | <td class="xl157169" align="right"><small>237.28</small></td> |
- | <td class="xl157169" align="right">4.746</td> | + | <td class="xl157169" align="right"><small>4.746</small></td> |
- | <td class="xl157169" align="right">2.517</td> | + | <td class="xl157169" align="right"><small>2.517</small></td> |
- | <td class="xl157169" align="right">1.89</td> | + | <td class="xl157169" align="right"><small>1.89</small></td> |
- | <td class="xl157169" align="right">2.12</td> | + | <td class="xl157169" align="right"><small>2.12</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl157169" style="height: 15pt;" | | <td class="xl157169" style="height: 15pt;" |
- | height="20">His-Tag</td> | + | height="20"><small>His-Tag</small></td> |
- | <td class="xl157169">FreiGEM</td> | + | <td class="xl157169"><small>FreiGEM</small></td> |
- | <td class="xl637169" align="right">22.07.2009</td> | + | <td class="xl637169" align="right"><small>22.07.2009</small></td> |
- | <td class="xl647169" align="right">19:06</td> | + | <td class="xl647169" align="right"><small>19:06</small></td> |
- | <td class="xl157169" align="right">31.22</td> | + | <td class="xl157169" align="right"><small>31.22</small></td> |
- | <td class="xl157169" align="right">0.624</td> | + | <td class="xl157169" align="right"><small>0.624</small></td> |
- | <td class="xl157169" align="right">0.369</td> | + | <td class="xl157169" align="right"><small>0.369</small></td> |
- | <td class="xl157169" align="right">1.69</td> | + | <td class="xl157169" align="right"><small>1.69</small></td> |
- | <td class="xl157169" align="right">0.42</td> | + | <td class="xl157169" align="right"><small>0.42</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl157169" style="height: 15pt;" | | <td class="xl157169" style="height: 15pt;" |
- | height="20">His-Tag. 2. Messung</td> | + | height="20"><small>His-Tag. 2. Messung</small></td> |
- | <td class="xl157169">FreiGEM</td> | + | <td class="xl157169"><small>FreiGEM</small></td> |
- | <td class="xl637169" align="right">22.07.2009</td> | + | <td class="xl637169" align="right"><small>22.07.2009</small></td> |
- | <td class="xl647169" align="right">19:07</td> | + | <td class="xl647169" align="right"><small>19:07</small></td> |
- | <td class="xl157169" align="right">23.24</td> | + | <td class="xl157169" align="right"><small>23.24</small></td> |
- | <td class="xl157169" align="right">0.465</td> | + | <td class="xl157169" align="right"><small>0.465</small></td> |
- | <td class="xl157169" align="right">0.245</td> | + | <td class="xl157169" align="right"><small>0.245</small></td> |
- | <td class="xl157169" align="right">1.9</td> | + | <td class="xl157169" align="right"><small>1.9</small></td> |
- | <td class="xl157169" align="right">0.35</td> | + | <td class="xl157169" align="right"><small>0.35</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl157169" style="height: 15pt;" | | <td class="xl157169" style="height: 15pt;" |
- | height="20">Strep-Tag</td> | + | height="20"><small>Strep-Tag</small></td> |
- | <td class="xl157169">FreiGEM</td> | + | <td class="xl157169"><small>FreiGEM</small></td> |
- | <td class="xl637169" align="right">22.07.2009</td> | + | <td class="xl637169" align="right"><small>22.07.2009</small></td> |
- | <td class="xl647169" align="right">19:08</td> | + | <td class="xl647169" align="right"><small>19:08</small></td> |
- | <td class="xl157169" align="right">25.79</td> | + | <td class="xl157169" align="right"><small>25.79</small></td> |
- | <td class="xl157169" align="right">0.516</td> | + | <td class="xl157169" align="right"><small>0.516</small></td> |
- | <td class="xl157169" align="right">0.26</td> | + | <td class="xl157169" align="right"><small>0.26</small></td> |
- | <td class="xl157169" align="right">1.99</td> | + | <td class="xl157169" align="right"><small>1.99</small></td> |
- | <td class="xl157169" align="right">0.29</td> | + | <td class="xl157169" align="right"><small>0.29</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl157169" style="height: 15pt;" | | <td class="xl157169" style="height: 15pt;" |
- | height="20">Lipocaline</td> | + | height="20"><small>Lipocaline</small></td> |
- | <td class="xl157169">FreiGEM</td> | + | <td class="xl157169"><small>FreiGEM</small></td> |
- | <td class="xl637169" align="right">22.07.2009</td> | + | <td class="xl637169" align="right"><small>22.07.2009</small></td> |
- | <td class="xl647169" align="right">19:09</td> | + | <td class="xl647169" align="right"><small>19:09</small></td> |
- | <td class="xl157169" align="right">28.57</td> | + | <td class="xl157169" align="right"><small>28.57</small></td> |
- | <td class="xl157169" align="right">0.571</td> | + | <td class="xl157169" align="right"><small>0.571</small></td> |
- | <td class="xl157169" align="right">0.326</td> | + | <td class="xl157169" align="right"><small>0.326</small></td> |
- | <td class="xl157169" align="right">1.75</td> | + | <td class="xl157169" align="right"><small>1.75</small></td> |
- | <td class="xl157169" align="right">0.32</td> | + | <td class="xl157169" align="right"><small>0.32</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl157169" style="height: 15pt;" | | <td class="xl157169" style="height: 15pt;" |
- | height="20">Digoxigenin</td> | + | height="20"><small>Digoxigenin</small></td> |
- | <td class="xl157169">FreiGEM</td> | + | <td class="xl157169"><small>FreiGEM</small></td> |
- | <td class="xl637169" align="right">22.07.2009</td> | + | <td class="xl637169" align="right"><small>22.07.2009</small></td> |
- | <td class="xl647169" align="right">19:10</td> | + | <td class="xl647169" align="right"><small>19:10</small></td> |
- | <td class="xl157169" align="right">16.75</td> | + | <td class="xl157169" align="right"><small>16.75</small></td> |
- | <td class="xl157169" align="right">0.335</td> | + | <td class="xl157169" align="right"><small>0.335</small></td> |
- | <td class="xl157169" align="right">0.195</td> | + | <td class="xl157169" align="right"><small>0.195</small></td> |
- | <td class="xl157169" align="right">1.72</td> | + | <td class="xl157169" align="right"><small>1.72</small></td> |
- | <td class="xl157169" align="right">0.29</td> | + | <td class="xl157169" align="right"><small>0.29</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl157169" style="height: 15pt;" | | <td class="xl157169" style="height: 15pt;" |
- | height="20">Lipocaline 2. Messung</td> | + | height="20"><small>Lipocaline 2. Messung</small></td> |
- | <td class="xl157169">FreiGEM</td> | + | <td class="xl157169"><small>FreiGEM</small></td> |
- | <td class="xl637169" align="right">22.07.2009</td> | + | <td class="xl637169" align="right"><small>22.07.2009</small></td> |
- | <td class="xl647169" align="right">19:11</td> | + | <td class="xl647169" align="right"><small>19:11</small></td> |
- | <td class="xl157169" align="right">6.86</td> | + | <td class="xl157169" align="right"><small>6.86</small></td> |
- | <td class="xl157169" align="right">0.137</td> | + | <td class="xl157169" align="right"><small>0.137</small></td> |
- | <td class="xl157169" align="right">0.064</td> | + | <td class="xl157169" align="right"><small>0.064</small></td> |
- | <td class="xl157169" align="right">2.15</td> | + | <td class="xl157169" align="right"><small>2.15</small></td> |
- | <td class="xl157169" align="right">0.14</td> | + | <td class="xl157169" align="right"><small>0.14</small></td> |
| </tr> | | </tr> |
| <tr style="display: none;" height="0"> | | <tr style="display: none;" height="0"> |
Line 2,873: |
Line 2,783: |
| <br /> | | <br /> |
| <ul> | | <ul> |
- | <li>preparation of Buffers for anion exchanger | + | <li>preparation of Buffers for anion exchanger </li> |
- | </li>
| + | |
| <li>picked colonies of the plates: Trafo FokA(21.07.09) and | | <li>picked colonies of the plates: Trafo FokA(21.07.09) and |
- | Ligation FokA-Split(20.07.09) and transfered into 3ml LB-Amp | + | Ligation FokA-Split(20.07.09) and transfered into 3ml LB-Amp </li> |
- | </li>
| + | |
| <li>check of LB-Amp plates:</li> | | <li>check of LB-Amp plates:</li> |
| </ul> | | </ul> |
- | Trafo GGGS(1a) 21.07.09 no growth <br /> | + | <div style="margin-left: 40px;"><small>Trafo |
| + | GGGS(1a) 21.07.09 no growth <br /> |
| Trafo GGGS(2a) 21.07.09 no growth<br /> | | Trafo GGGS(2a) 21.07.09 no growth<br /> |
| Trafo GGGS(1b) 21.07.09 no growth <br /> | | Trafo GGGS(1b) 21.07.09 no growth <br /> |
Line 2,889: |
Line 2,798: |
| Ligation FokA+Split 20.07.09 2 colonies<br /> | | Ligation FokA+Split 20.07.09 2 colonies<br /> |
| Trafo FokA 21.07.09 some colonies+satellite colonies stored in | | Trafo FokA 21.07.09 some colonies+satellite colonies stored in |
- | refrigerator(Premium) | + | refrigerator(Premium)</small> |
| + | </div> |
| <h3>23.07.09, Sarah, Isabel</h3> | | <h3>23.07.09, Sarah, Isabel</h3> |
| <br /> | | <br /> |
| <ul> | | <ul> |
- | <li> complete precipitation of phage M13 particles | + | <li> complete precipitation of phage M13 particles </li> |
- | </li>
| + | <li> phage stock in -80°C fridge </li> |
- | <li> phage stock in -80°C fridge | + | <li> phage M13 ssDNA precipitation and purification </li> |
- | </li>
| + | <li> ssDNA in -20°C fridge </li> |
- | <li> phage M13 ssDNA precipitation and purification | + | |
- | </li>
| + | |
- | <li> ssDNA in -20°C fridge | + | |
- | </li>
| + | |
| </ul> | | </ul> |
| <h3>23.07.09, Manu, Laura</h3> | | <h3>23.07.09, Manu, Laura</h3> |
| <br /> | | <br /> |
| <ul> | | <ul> |
- | <li>design and order of Gen-III Primers | + | <li>design and order of Gen-III Primers </li> |
- | </li>
| + | |
| <li>design and order of Short-, Middle- and Long-Linkers for | | <li>design and order of Short-, Middle- and Long-Linkers for |
- | Fok | + | Fok </li> |
- | </li>
| + | |
| </ul> | | </ul> |
| <h3>23.07.09, Hannes 11:00-17:00</h3> | | <h3>23.07.09, Hannes 11:00-17:00</h3> |
Line 2,916: |
Line 2,820: |
| <li>transformation of ligation from 22.07.09:</li> | | <li>transformation of ligation from 22.07.09:</li> |
| </ul> | | </ul> |
- | <div style="margin-left: 40px;">- 50µl XBlue + | + | <div style="margin-left: 40px;"><small>- |
| + | 50µl XBlue + |
| 5µl ligation His-Lipo<br /> | | 5µl ligation His-Lipo<br /> |
| - 50µl Xblue + 5µl ligation His-Dig<br /> | | - 50µl Xblue + 5µl ligation His-Dig<br /> |
Line 2,923: |
Line 2,828: |
| --> plate out 2 plates: 1. 50µl cells; 2. pellet | | --> plate out 2 plates: 1. 50µl cells; 2. pellet |
| (5min, 2000rpm) dissolved in 50µl LB<br /> | | (5min, 2000rpm) dissolved in 50µl LB<br /> |
- | --> 37°C o/n<br /> | + | --> 37°C o/n</small><br /> |
| </div> | | </div> |
| <br /> | | <br /> |
Line 2,930: |
Line 2,835: |
| <li>transformation to check competent cells:</li> | | <li>transformation to check competent cells:</li> |
| </ul> | | </ul> |
- | <div style="margin-left: 40px;">- 50µl XBlue + | + | <div style="margin-left: 40px;"><small>- |
| + | 50µl XBlue + |
| 2µl pUC<br /> | | 2µl pUC<br /> |
| - 50µl T7 + 2µl pUC<br /> | | - 50µl T7 + 2µl pUC<br /> |
| - 50µl BL21 de3 (Novagen) + 2µl pUC<br /> | | - 50µl BL21 de3 (Novagen) + 2µl pUC<br /> |
| - 50µl RV308 + 2µl pUC<br /> | | - 50µl RV308 + 2µl pUC<br /> |
- | --> plate out 10µl cells + 40µl LB <strong | + | --> plate out 10µl cells + 40µl LB <strong>was |
- | ="red">was supposed to be 100 µL cells only,
| + | supposed to be 100 µL cells only, |
- | please repeat & plate all cell suspension (Tobias)</strong><br /> | + | please repeat & plate all cell suspension (Tobias)</strong></small><br /> |
- | <strong ="red"></strong></div> | + | <strong></strong></div> |
| <br /> | | <br /> |
| <br /> | | <br /> |
Line 2,944: |
Line 2,850: |
| <li>Inoculation for stocks and competent cells:</li> | | <li>Inoculation for stocks and competent cells:</li> |
| </ul> | | </ul> |
- | <div style="margin-left: 40px;">- 15ml LB+Chloramphenicol | + | <div style="margin-left: 40px;"><small>- 15ml |
| + | LB+Chloramphenicol |
| with T7 (competent cells)<br /> | | with T7 (competent cells)<br /> |
| - 15ml LB+tet with XBlue<br /> | | - 15ml LB+tet with XBlue<br /> |
| - 15ml LB BL21 de3<br /> | | - 15ml LB BL21 de3<br /> |
- | --> 37°C o/n | + | --> 37°C o/n</small> |
| </div> | | </div> |
| <h3>24.07.09, Hannes, Christoph, Manuel</h3> | | <h3>24.07.09, Hannes, Christoph, Manuel</h3> |
Line 2,956: |
Line 2,863: |
| - BL 21 de3 + pUC: 0 colonies</li> | | - BL 21 de3 + pUC: 0 colonies</li> |
| </ul> | | </ul> |
- | <div style="margin-left: 40px;">- RV308 + pUC: 11 colonies<br /> | + | <div style="margin-left: 40px;"><small>- RV308 + |
| + | pUC: 11 colonies<br /> |
| - T7 + pUC: 67 colonies<br /> | | - T7 + pUC: 67 colonies<br /> |
| - XL1blue + pUC: 0 colonies<br /> | | - XL1blue + pUC: 0 colonies<br /> |
| - ligation of Dig+His, Dig+Strep, FluA+His, FluA+Strep was covered with | | - ligation of Dig+His, Dig+Strep, FluA+His, FluA+Strep was covered with |
| many colonies | | many colonies |
- | <br /> | + | </small><br /> |
| </div> | | </div> |
| <ul> | | <ul> |
- | <li> miniprep of FokA and FokA+split | + | <li> miniprep of FokA and FokA+split </li> |
- | </li>
| + | |
| <li> Glycerol stocks (30%) of: | | <li> Glycerol stocks (30%) of: |
| - T7</li> | | - T7</li> |
| </ul> | | </ul> |
- | <div style="margin-left: 40px;">- XBlue<br /> | + | <div style="margin-left: 40px;"><small>- XBlue<br /> |
| - BL21 de3<br /> | | - BL21 de3<br /> |
- | </div> | + | </small></div> |
- | <div style="margin-left: 40px;">--> -80°C<br /> | + | <div style="margin-left: 40px;"><small>--> |
| + | -80°C</small><br /> |
| </div> | | </div> |
| <br /> | | <br /> |
Line 2,985: |
Line 2,893: |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl1519903" style="height: 15pt; width: 60pt;" | | <td class="xl1519903" style="height: 15pt; width: 60pt;" |
- | height="20" width="80">Sample | + | height="20" width="80"><small>Sample |
- | ID</td> | + | ID</small></td> |
| <td class="xl1519903" style="width: 48pt;" | | <td class="xl1519903" style="width: 48pt;" |
- | width="64">User ID</td> | + | width="64"><small>User ID</small></td> |
| <td class="xl1519903" style="width: 53pt;" | | <td class="xl1519903" style="width: 53pt;" |
- | width="71">Date<span style=""> </span></td> | + | width="71"><small>Date<span style=""> </span></small></td> |
| <td class="xl1519903" style="width: 48pt;" | | <td class="xl1519903" style="width: 48pt;" |
- | width="64">Time<span style=""> </span></td> | + | width="64"><small>Time<span style=""> </span></small></td> |
| <td class="xl1519903" style="width: 48pt;" | | <td class="xl1519903" style="width: 48pt;" |
- | width="64">ng/ul<span style=""> </span></td> | + | width="64"><small>ng/ul<span style=""> </span></small></td> |
| <td class="xl1519903" style="width: 48pt;" | | <td class="xl1519903" style="width: 48pt;" |
- | width="64">A260<span style=""> </span></td> | + | width="64"><small>A260<span style=""> </span></small></td> |
| <td class="xl1519903" style="width: 66pt;" | | <td class="xl1519903" style="width: 66pt;" |
- | width="88">A280<span style=""> </span></td> | + | width="88"><small>A280<span style=""> </span></small></td> |
| <td class="xl1519903" style="width: 71pt;" | | <td class="xl1519903" style="width: 71pt;" |
- | width="94">260/280<span style=""> </span></td> | + | width="94"><small>260/280<span style=""> </span></small></td> |
| <td class="xl1519903" style="width: 65pt;" | | <td class="xl1519903" style="width: 65pt;" |
- | width="87">260/230<span style=""> </span></td> | + | width="87"><small>260/230<span style=""> </span></small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl1519903" style="height: 15pt;" | | <td class="xl1519903" style="height: 15pt;" |
- | height="20">FOKa</td> | + | height="20"><small>FOKa</small></td> |
- | <td class="xl1519903">FreiGEM</td> | + | <td class="xl1519903"><small>FreiGEM</small></td> |
- | <td class="xl6319903" align="right">24.07.2009</td> | + | <td class="xl6319903" align="right"><small>24.07.2009</small></td> |
- | <td class="xl6419903" align="right">10:24</td> | + | <td class="xl6419903" align="right"><small>10:24</small></td> |
- | <td class="xl1519903" align="right">189.39</td> | + | <td class="xl1519903" align="right"><small>189.39</small></td> |
- | <td class="xl1519903" align="right">3.788</td> | + | <td class="xl1519903" align="right"><small>3.788</small></td> |
- | <td class="xl1519903" align="right">2.009</td> | + | <td class="xl1519903" align="right"><small>2.009</small></td> |
- | <td class="xl1519903" align="right">1.89</td> | + | <td class="xl1519903" align="right"><small>1.89</small></td> |
- | <td class="xl1519903" align="right">2.21</td> | + | <td class="xl1519903" align="right"><small>2.21</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl1519903" style="height: 15pt;" | | <td class="xl1519903" style="height: 15pt;" |
- | height="20">FOKa + split</td> | + | height="20"><small>FOKa + split</small></td> |
- | <td class="xl1519903">FreiGEM</td> | + | <td class="xl1519903"><small>FreiGEM</small></td> |
- | <td class="xl6319903" align="right">24.07.2009</td> | + | <td class="xl6319903" align="right"><small>24.07.2009</small></td> |
- | <td class="xl6419903" align="right">10:25</td> | + | <td class="xl6419903" align="right"><small>10:25</small></td> |
- | <td class="xl1519903" align="right">253.3</td> | + | <td class="xl1519903" align="right"><small>253.3</small></td> |
- | <td class="xl1519903" align="right">5.066</td> | + | <td class="xl1519903" align="right"><small>5.066</small></td> |
- | <td class="xl1519903" align="right">2.679</td> | + | <td class="xl1519903" align="right"><small>2.679</small></td> |
- | <td class="xl1519903" align="right">1.89</td> | + | <td class="xl1519903" align="right"><small>1.89</small></td> |
- | <td class="xl1519903" align="right">2.21</td> | + | <td class="xl1519903" align="right"><small>2.21</small></td> |
| </tr> | | </tr> |
| <tr style="display: none;" height="0"> | | <tr style="display: none;" height="0"> |
Line 3,044: |
Line 2,952: |
| <br /> | | <br /> |
| <ul> | | <ul> |
- | <li>Inoculation of His-Dig, His-Lipo, Strep-Dig, Strep-Lipo | + | <li>Inoculation of His-Dig, His-Lipo, Strep-Dig, Strep-Lipo </li> |
- | </li>
| + | |
| <li>preparation of 100 ml LB+Chloramphenicol and 100ml | | <li>preparation of 100 ml LB+Chloramphenicol and 100ml |
- | LB+Tetracyclin | + | LB+Tetracyclin </li> |
- | </li>
| + | |
| <li>Inoclulation of XL blue1, T7 Express in 5 ml LB+antibiotics | | <li>Inoclulation of XL blue1, T7 Express in 5 ml LB+antibiotics |
| </li> | | </li> |
| <li>order of 2 oligos for the M13mp18 ss DNA (there are 4 Fok | | <li>order of 2 oligos for the M13mp18 ss DNA (there are 4 Fok |
| restriction sites) to cut at two sites resulting in fragments of about | | restriction sites) to cut at two sites resulting in fragments of about |
- | 890 and 6360 bp with Fok | + | 890 and 6360 bp with Fok </li> |
- | </li>
| + | |
| </ul> | | </ul> |
| <h3>28.07.09, Dieter, Max</h3> | | <h3>28.07.09, Dieter, Max</h3> |
Line 3,060: |
Line 2,965: |
| <ul> | | <ul> |
| <li>Inoculation of His-Dig, His-Lipo, Strep-Dig, Strep-Lipo, | | <li>Inoculation of His-Dig, His-Lipo, Strep-Dig, Strep-Lipo, |
- | Foki-Split in 5 ml LB+antibiotics (this time on the shaker) | + | Foki-Split in 5 ml LB+antibiotics (this time on the shaker) </li> |
- | </li>
| + | <li>preparation of 75 ml LB+Ampicilin </li> |
- | <li>preparation of 75 ml LB+Ampicilin | + | |
- | </li>
| + | |
| <li>Inoclulation of XL blue1, T7 Express in 5 ml LB+antibiotics</li> | | <li>Inoclulation of XL blue1, T7 Express in 5 ml LB+antibiotics</li> |
- | <li>The Primers for Gene III have arrived | + | <li>The Primers for Gene III have arrived </li> |
- | </li>
| + | |
| </ul> | | </ul> |
| <h3>29.07.09, Isabel, Sarah, Gerrit, Dieter, Laura</h3> | | <h3>29.07.09, Isabel, Sarah, Gerrit, Dieter, Laura</h3> |
Line 3,074: |
Line 2,976: |
| Strep-Lipo, Foki-Split -> very low concentrations except for | | Strep-Lipo, Foki-Split -> very low concentrations except for |
| His-Lipo and Strep-Dig (just low) -> made a digest with both and | | His-Lipo and Strep-Dig (just low) -> made a digest with both and |
- | pMal-CAT (9.7. clon 2.1) | + | pMal-CAT (9.7. clon 2.1) </li> |
- | </li>
| + | |
| <li>new inoculations of His-Dig, His-Lipo, Strep-Dig, | | <li>new inoculations of His-Dig, His-Lipo, Strep-Dig, |
- | Strep-Lipo, Foki-Split | + | Strep-Lipo, Foki-Split </li> |
- | </li>
| + | |
| <li>we also made competent cells of T7 and XL Blue (about 50 | | <li>we also made competent cells of T7 and XL Blue (about 50 |
- | aliquots each) | + | aliquots each) </li> |
- | </li>
| + | <li>we poured Amp-plates </li> |
- | <li>we poured Amp-plates | + | <li>complementary linker and GenIII-Primer arrived </li> |
- | </li>
| + | |
- | <li>complementary linker and GenIII-Primer arrived | + | |
- | </li>
| + | |
| <li><b>Nanodrop data</b></li> | | <li><b>Nanodrop data</b></li> |
| </ul> | | </ul> |
Line 3,095: |
Line 2,992: |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl651320" style="height: 15pt; width: 95pt;" | | <td class="xl651320" style="height: 15pt; width: 95pt;" |
- | height="20" width="127">Sample | + | height="20" width="127"><small>Sample |
- | ID</td> | + | ID</small></td> |
| <td class="xl651320" style="width: 48pt;" | | <td class="xl651320" style="width: 48pt;" |
- | width="64">User ID</td> | + | width="64"><small>User ID</small></td> |
| <td class="xl651320" style="width: 53pt;" | | <td class="xl651320" style="width: 53pt;" |
- | width="71">Date<span style=""> </span></td> | + | width="71"><small>Date<span style=""> </span></small></td> |
| <td class="xl651320" style="width: 48pt;" | | <td class="xl651320" style="width: 48pt;" |
- | width="64">Time<span style=""> </span></td> | + | width="64"><small>Time<span style=""> </span></small></td> |
| <td class="xl651320" style="width: 48pt;" | | <td class="xl651320" style="width: 48pt;" |
- | width="64">ng/ul<span style=""> </span></td> | + | width="64"><small>ng/ul<span style=""> </span></small></td> |
| <td class="xl651320" style="width: 48pt;" | | <td class="xl651320" style="width: 48pt;" |
- | width="64">A260<span style=""> </span></td> | + | width="64"><small>A260<span style=""> </span></small></td> |
| <td class="xl651320" style="width: 66pt;" | | <td class="xl651320" style="width: 66pt;" |
- | width="88">A280<span style=""> </span></td> | + | width="88"><small>A280<span style=""> </span></small></td> |
| <td class="xl651320" style="width: 71pt;" | | <td class="xl651320" style="width: 71pt;" |
- | width="94">260/280<span style=""> </span></td> | + | width="94"><small>260/280<span style=""> </span></small></td> |
| <td class="xl651320" style="width: 65pt;" | | <td class="xl651320" style="width: 65pt;" |
- | width="87">260/230<span style=""> </span></td> | + | width="87"><small>260/230<span style=""> </span></small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl151320" style="height: 15pt;" | | <td class="xl151320" style="height: 15pt;" |
- | height="20">Foki+Split clone 4</td> | + | height="20"><small>Foki+Split clone 4</small></td> |
- | <td class="xl151320">FreiGEM</td> | + | <td class="xl151320"><small>FreiGEM</small></td> |
- | <td class="xl631320" align="right">29.07.2009</td> | + | <td class="xl631320" align="right"><small>29.07.2009</small></td> |
- | <td class="xl641320" align="right">13:08</td> | + | <td class="xl641320" align="right"><small>13:08</small></td> |
- | <td class="xl151320" align="right">5.12</td> | + | <td class="xl151320" align="right"><small>5.12</small></td> |
- | <td class="xl151320" align="right">0.102</td> | + | <td class="xl151320" align="right"><small>0.102</small></td> |
- | <td class="xl151320" align="right">0.063</td> | + | <td class="xl151320" align="right"><small>0.063</small></td> |
- | <td class="xl151320" align="right">1.62</td> | + | <td class="xl151320" align="right"><small>1.62</small></td> |
- | <td class="xl151320" align="right">2.07</td> | + | <td class="xl151320" align="right"><small>2.07</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl151320" style="height: 15pt;" | | <td class="xl151320" style="height: 15pt;" |
- | height="20">Strep+Dig clone 3</td> | + | height="20"><small>Strep+Dig clone 3</small></td> |
- | <td class="xl151320">FreiGEM</td> | + | <td class="xl151320"><small>FreiGEM</small></td> |
- | <td class="xl631320" align="right">29.07.2009</td> | + | <td class="xl631320" align="right"><small>29.07.2009</small></td> |
- | <td class="xl641320" align="right">13:09</td> | + | <td class="xl641320" align="right"><small>13:09</small></td> |
- | <td class="xl151320" align="right">4.26</td> | + | <td class="xl151320" align="right"><small>4.26</small></td> |
- | <td class="xl151320" align="right">0.085</td> | + | <td class="xl151320" align="right"><small>0.085</small></td> |
- | <td class="xl151320" align="right">0.065</td> | + | <td class="xl151320" align="right"><small>0.065</small></td> |
- | <td class="xl151320" align="right">1.32</td> | + | <td class="xl151320" align="right"><small>1.32</small></td> |
- | <td class="xl151320" align="right">1.22</td> | + | <td class="xl151320" align="right"><small>1.22</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl151320" style="height: 15pt;" | | <td class="xl151320" style="height: 15pt;" |
- | height="20">His+Lipo clone 4</td> | + | height="20"><small>His+Lipo clone 4</small></td> |
- | <td class="xl151320">FreiGEM</td> | + | <td class="xl151320"><small>FreiGEM</small></td> |
- | <td class="xl631320" align="right">29.07.2009</td> | + | <td class="xl631320" align="right"><small>29.07.2009</small></td> |
- | <td class="xl641320" align="right">13:10</td> | + | <td class="xl641320" align="right"><small>13:10</small></td> |
- | <td class="xl151320" align="right">8.06</td> | + | <td class="xl151320" align="right"><small>8.06</small></td> |
- | <td class="xl151320" align="right">0.161</td> | + | <td class="xl151320" align="right"><small>0.161</small></td> |
- | <td class="xl151320" align="right">0.095</td> | + | <td class="xl151320" align="right"><small>0.095</small></td> |
- | <td class="xl151320" align="right">1.69</td> | + | <td class="xl151320" align="right"><small>1.69</small></td> |
- | <td class="xl151320" align="right">1.44</td> | + | <td class="xl151320" align="right"><small>1.44</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl151320" style="height: 15pt;" | | <td class="xl151320" style="height: 15pt;" |
- | height="20">Strep+Lipo clone 4</td> | + | height="20"><small>Strep+Lipo clone 4</small></td> |
- | <td class="xl151320">FreiGEM</td> | + | <td class="xl151320"><small>FreiGEM</small></td> |
- | <td class="xl631320" align="right">29.07.2009</td> | + | <td class="xl631320" align="right"><small>29.07.2009</small></td> |
- | <td class="xl641320" align="right">13:11</td> | + | <td class="xl641320" align="right"><small>13:11</small></td> |
- | <td class="xl151320" align="right">5.14</td> | + | <td class="xl151320" align="right"><small>5.14</small></td> |
- | <td class="xl151320" align="right">0.103</td> | + | <td class="xl151320" align="right"><small>0.103</small></td> |
- | <td class="xl151320" align="right">0.078</td> | + | <td class="xl151320" align="right"><small>0.078</small></td> |
- | <td class="xl151320" align="right">1.31</td> | + | <td class="xl151320" align="right"><small>1.31</small></td> |
- | <td class="xl151320" align="right">1.73</td> | + | <td class="xl151320" align="right"><small>1.73</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl151320" style="height: 15pt;" | | <td class="xl151320" style="height: 15pt;" |
- | height="20">Strep+Lipo clone 3</td> | + | height="20"><small>Strep+Lipo clone 3</small></td> |
- | <td class="xl151320">FreiGEM</td> | + | <td class="xl151320"><small>FreiGEM</small></td> |
- | <td class="xl631320" align="right">29.07.2009</td> | + | <td class="xl631320" align="right"><small>29.07.2009</small></td> |
- | <td class="xl641320" align="right">13:12</td> | + | <td class="xl641320" align="right"><small>13:12</small></td> |
- | <td class="xl151320" align="right">5.52</td> | + | <td class="xl151320" align="right"><small>5.52</small></td> |
- | <td class="xl151320" align="right">0.11</td> | + | <td class="xl151320" align="right"><small>0.11</small></td> |
- | <td class="xl151320" align="right">0.073</td> | + | <td class="xl151320" align="right"><small>0.073</small></td> |
- | <td class="xl151320" align="right">1.5</td> | + | <td class="xl151320" align="right"><small>1.5</small></td> |
- | <td class="xl151320" align="right">2.65</td> | + | <td class="xl151320" align="right"><small>2.65</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl151320" style="height: 15pt;" | | <td class="xl151320" style="height: 15pt;" |
- | height="20">His+Lipo clone 3</td> | + | height="20"><small>His+Lipo clone 3</small></td> |
- | <td class="xl151320">FreiGEM</td> | + | <td class="xl151320"><small>FreiGEM</small></td> |
- | <td class="xl631320" align="right">29.07.2009</td> | + | <td class="xl631320" align="right"><small>29.07.2009</small></td> |
- | <td class="xl641320" align="right">13:12</td> | + | <td class="xl641320" align="right"><small>13:12</small></td> |
- | <td class="xl151320" align="right">3.37</td> | + | <td class="xl151320" align="right"><small>3.37</small></td> |
- | <td class="xl151320" align="right">0.067</td> | + | <td class="xl151320" align="right"><small>0.067</small></td> |
- | <td class="xl151320" align="right">0.03</td> | + | <td class="xl151320" align="right"><small>0.03</small></td> |
- | <td class="xl151320" align="right">2.23</td> | + | <td class="xl151320" align="right"><small>2.23</small></td> |
- | <td class="xl151320" align="right">2.6</td> | + | <td class="xl151320" align="right"><small>2.6</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl151320" style="height: 15pt;" | | <td class="xl151320" style="height: 15pt;" |
- | height="20">Foki+Split clone 3</td> | + | height="20"><small>Foki+Split clone 3</small></td> |
- | <td class="xl151320">FreiGEM</td> | + | <td class="xl151320"><small>FreiGEM</small></td> |
- | <td class="xl631320" align="right">29.07.2009</td> | + | <td class="xl631320" align="right"><small>29.07.2009</small></td> |
- | <td class="xl641320" align="right">13:13</td> | + | <td class="xl641320" align="right"><small>13:13</small></td> |
- | <td class="xl151320" align="right">3.61</td> | + | <td class="xl151320" align="right"><small>3.61</small></td> |
- | <td class="xl151320" align="right">0.072</td> | + | <td class="xl151320" align="right"><small>0.072</small></td> |
- | <td class="xl151320" align="right">0.051</td> | + | <td class="xl151320" align="right"><small>0.051</small></td> |
- | <td class="xl151320" align="right">1.41</td> | + | <td class="xl151320" align="right"><small>1.41</small></td> |
- | <td class="xl151320" align="right">1.49</td> | + | <td class="xl151320" align="right"><small>1.49</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl151320" style="height: 15pt;" | | <td class="xl151320" style="height: 15pt;" |
- | height="20">Strep+Dig clone 4</td> | + | height="20"><small>Strep+Dig clone 4</small></td> |
- | <td class="xl151320">FreiGEM</td> | + | <td class="xl151320"><small>FreiGEM</small></td> |
- | <td class="xl631320" align="right">29.07.2009</td> | + | <td class="xl631320" align="right"><small>29.07.2009</small></td> |
- | <td class="xl641320" align="right">13:14</td> | + | <td class="xl641320" align="right"><small>13:14</small></td> |
- | <td class="xl151320" align="right">4.77</td> | + | <td class="xl151320" align="right"><small>4.77</small></td> |
- | <td class="xl151320" align="right">0.095</td> | + | <td class="xl151320" align="right"><small>0.095</small></td> |
- | <td class="xl151320" align="right">0.038</td> | + | <td class="xl151320" align="right"><small>0.038</small></td> |
- | <td class="xl151320" align="right">2.51</td> | + | <td class="xl151320" align="right"><small>2.51</small></td> |
- | <td class="xl151320" align="right">1.12</td> | + | <td class="xl151320" align="right"><small>1.12</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl151320" style="height: 15pt;" | | <td class="xl151320" style="height: 15pt;" |
- | height="20">His+Dig clone 4</td> | + | height="20"><small>His+Dig clone 4</small></td> |
- | <td class="xl151320">FreiGEM</td> | + | <td class="xl151320"><small>FreiGEM</small></td> |
- | <td class="xl631320" align="right">29.07.2009</td> | + | <td class="xl631320" align="right"><small>29.07.2009</small></td> |
- | <td class="xl641320" align="right">13:16</td> | + | <td class="xl641320" align="right"><small>13:16</small></td> |
- | <td class="xl151320" align="right">12.5</td> | + | <td class="xl151320" align="right"><small>12.5</small></td> |
- | <td class="xl151320" align="right">0.25</td> | + | <td class="xl151320" align="right"><small>0.25</small></td> |
- | <td class="xl151320" align="right">0.174</td> | + | <td class="xl151320" align="right"><small>0.174</small></td> |
- | <td class="xl151320" align="right">1.43</td> | + | <td class="xl151320" align="right"><small>1.43</small></td> |
- | <td class="xl151320" align="right">0.85</td> | + | <td class="xl151320" align="right"><small>0.85</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl151320" style="height: 15pt;" | | <td class="xl151320" style="height: 15pt;" |
- | height="20">His+Dig clone 4_2</td> | + | height="20"><small>His+Dig clone 4_2</small></td> |
- | <td class="xl151320">FreiGEM</td> | + | <td class="xl151320"><small>FreiGEM</small></td> |
- | <td class="xl631320" align="right">29.07.2009</td> | + | <td class="xl631320" align="right"><small>29.07.2009</small></td> |
- | <td class="xl641320" align="right">13:16</td> | + | <td class="xl641320" align="right"><small>13:16</small></td> |
- | <td class="xl151320" align="right">3.01</td> | + | <td class="xl151320" align="right"><small>3.01</small></td> |
- | <td class="xl151320" align="right">0.06</td> | + | <td class="xl151320" align="right"><small>0.06</small></td> |
- | <td class="xl151320" align="right">0.044</td> | + | <td class="xl151320" align="right"><small>0.044</small></td> |
- | <td class="xl151320" align="right">1.36</td> | + | <td class="xl151320" align="right"><small>1.36</small></td> |
- | <td class="xl151320" align="right">1.45</td> | + | <td class="xl151320" align="right"><small>1.45</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl151320" style="height: 15pt;" | | <td class="xl151320" style="height: 15pt;" |
- | height="20">Foki+Split clone 4</td> | + | height="20"><small>Foki+Split clone 4</small></td> |
- | <td class="xl151320">FreiGEM</td> | + | <td class="xl151320"><small>FreiGEM</small></td> |
- | <td class="xl631320" align="right">29.07.2009</td> | + | <td class="xl631320" align="right"><small>29.07.2009</small></td> |
- | <td class="xl641320" align="right">13:17</td> | + | <td class="xl641320" align="right"><small>13:17</small></td> |
- | <td class="xl151320" align="right">3.44</td> | + | <td class="xl151320" align="right"><small>3.44</small></td> |
- | <td class="xl151320" align="right">0.069</td> | + | <td class="xl151320" align="right"><small>0.069</small></td> |
- | <td class="xl151320" align="right">0.034</td> | + | <td class="xl151320" align="right"><small>0.034</small></td> |
- | <td class="xl151320" align="right">2.05</td> | + | <td class="xl151320" align="right"><small>2.05</small></td> |
- | <td class="xl151320" align="right">5.96</td> | + | <td class="xl151320" align="right"><small>5.96</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl151320" style="height: 15pt;" | | <td class="xl151320" style="height: 15pt;" |
- | height="20">Strep+Dig clone 3</td> | + | height="20"><small>Strep+Dig clone 3</small></td> |
- | <td class="xl151320">FreiGEM</td> | + | <td class="xl151320"><small>FreiGEM</small></td> |
- | <td class="xl631320" align="right">29.07.2009</td> | + | <td class="xl631320" align="right"><small>29.07.2009</small></td> |
- | <td class="xl641320" align="right">13:18</td> | + | <td class="xl641320" align="right"><small>13:18</small></td> |
- | <td class="xl151320" align="right">5.03</td> | + | <td class="xl151320" align="right"><small>5.03</small></td> |
- | <td class="xl151320" align="right">0.101</td> | + | <td class="xl151320" align="right"><small>0.101</small></td> |
- | <td class="xl151320" align="right">0.051</td> | + | <td class="xl151320" align="right"><small>0.051</small></td> |
- | <td class="xl151320" align="right">1.96</td> | + | <td class="xl151320" align="right"><small>1.96</small></td> |
- | <td class="xl151320" align="right">1.44</td> | + | <td class="xl151320" align="right"><small>1.44</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl151320" style="height: 15pt;" | | <td class="xl151320" style="height: 15pt;" |
- | height="20">His+Lipo clone 4</td> | + | height="20"><small>His+Lipo clone 4</small></td> |
- | <td class="xl151320">FreiGEM</td> | + | <td class="xl151320"><small>FreiGEM</small></td> |
- | <td class="xl631320" align="right">29.07.2009</td> | + | <td class="xl631320" align="right"><small>29.07.2009</small></td> |
- | <td class="xl641320" align="right">13:18</td> | + | <td class="xl641320" align="right"><small>13:18</small></td> |
- | <td class="xl151320" align="right">83.39</td> | + | <td class="xl151320" align="right"><small>83.39</small></td> |
- | <td class="xl151320" align="right">1.668</td> | + | <td class="xl151320" align="right"><small>1.668</small></td> |
- | <td class="xl151320" align="right">1.09</td> | + | <td class="xl151320" align="right"><small>1.09</small></td> |
- | <td class="xl151320" align="right">1.53</td> | + | <td class="xl151320" align="right"><small>1.53</small></td> |
- | <td class="xl151320" align="right">0.66</td> | + | <td class="xl151320" align="right"><small>0.66</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl151320" style="height: 15pt;" | | <td class="xl151320" style="height: 15pt;" |
- | height="20">Strep+Lipo clone 4</td> | + | height="20"><small>Strep+Lipo clone 4</small></td> |
- | <td class="xl151320">FreiGEM</td> | + | <td class="xl151320"><small>FreiGEM</small></td> |
- | <td class="xl631320" align="right">29.07.2009</td> | + | <td class="xl631320" align="right"><small>29.07.2009</small></td> |
- | <td class="xl641320" align="right">13:20</td> | + | <td class="xl641320" align="right"><small>13:20</small></td> |
- | <td class="xl151320" align="right">36.95</td> | + | <td class="xl151320" align="right"><small>36.95</small></td> |
- | <td class="xl151320" align="right">0.739</td> | + | <td class="xl151320" align="right"><small>0.739</small></td> |
- | <td class="xl151320" align="right">0.458</td> | + | <td class="xl151320" align="right"><small>0.458</small></td> |
- | <td class="xl151320" align="right">1.61</td> | + | <td class="xl151320" align="right"><small>1.61</small></td> |
- | <td class="xl151320" align="right">0.79</td> | + | <td class="xl151320" align="right"><small>0.79</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl151320" style="height: 15pt;" | | <td class="xl151320" style="height: 15pt;" |
- | height="20">Strep+Lipo clone 3</td> | + | height="20"><small>Strep+Lipo clone 3</small></td> |
- | <td class="xl151320">FreiGEM</td> | + | <td class="xl151320"><small>FreiGEM</small></td> |
- | <td class="xl631320" align="right">29.07.2009</td> | + | <td class="xl631320" align="right"><small>29.07.2009</small></td> |
- | <td class="xl641320" align="right">13:21</td> | + | <td class="xl641320" align="right"><small>13:21</small></td> |
- | <td class="xl151320" align="right">15</td> | + | <td class="xl151320" align="right"><small>15</small></td> |
- | <td class="xl151320" align="right">0.3</td> | + | <td class="xl151320" align="right"><small>0.3</small></td> |
- | <td class="xl151320" align="right">0.181</td> | + | <td class="xl151320" align="right"><small>0.181</small></td> |
- | <td class="xl151320" align="right">1.66</td> | + | <td class="xl151320" align="right"><small>1.66</small></td> |
- | <td class="xl151320" align="right">0.84</td> | + | <td class="xl151320" align="right"><small>0.84</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl151320" style="height: 15pt;" | | <td class="xl151320" style="height: 15pt;" |
- | height="20">His+Lipo clone 3</td> | + | height="20"><small>His+Lipo clone 3</small></td> |
- | <td class="xl151320">FreiGEM</td> | + | <td class="xl151320"><small>FreiGEM</small></td> |
- | <td class="xl631320" align="right">29.07.2009</td> | + | <td class="xl631320" align="right"><small>29.07.2009</small></td> |
- | <td class="xl641320" align="right">13:22</td> | + | <td class="xl641320" align="right"><small>13:22</small></td> |
- | <td class="xl151320" align="right">99.09</td> | + | <td class="xl151320" align="right"><small>99.09</small></td> |
- | <td class="xl151320" align="right">1.982</td> | + | <td class="xl151320" align="right"><small>1.982</small></td> |
- | <td class="xl151320" align="right">1.316</td> | + | <td class="xl151320" align="right"><small>1.316</small></td> |
- | <td class="xl151320" align="right">1.51</td> | + | <td class="xl151320" align="right"><small>1.51</small></td> |
- | <td class="xl151320" align="right">0.61</td> | + | <td class="xl151320" align="right"><small>0.61</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl151320" style="height: 15pt;" | | <td class="xl151320" style="height: 15pt;" |
- | height="20">His+Lipo clone 3</td> | + | height="20"><small>His+Lipo clone 3</small></td> |
- | <td class="xl151320">FreiGEM</td> | + | <td class="xl151320"><small>FreiGEM</small></td> |
- | <td class="xl631320" align="right">29.07.2009</td> | + | <td class="xl631320" align="right"><small>29.07.2009</small></td> |
- | <td class="xl641320" align="right">13:23</td> | + | <td class="xl641320" align="right"><small>13:23</small></td> |
- | <td class="xl151320" align="right">21.34</td> | + | <td class="xl151320" align="right"><small>21.34</small></td> |
- | <td class="xl151320" align="right">0.427</td> | + | <td class="xl151320" align="right"><small>0.427</small></td> |
- | <td class="xl151320" align="right">0.301</td> | + | <td class="xl151320" align="right"><small>0.301</small></td> |
- | <td class="xl151320" align="right">1.42</td> | + | <td class="xl151320" align="right"><small>1.42</small></td> |
- | <td class="xl151320" align="right">0.68</td> | + | <td class="xl151320" align="right"><small>0.68</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl151320" style="height: 15pt;" | | <td class="xl151320" style="height: 15pt;" |
- | height="20">Foki+Split clone 3</td> | + | height="20"><small>Foki+Split clone 3</small></td> |
- | <td class="xl151320">FreiGEM</td> | + | <td class="xl151320"><small>FreiGEM</small></td> |
- | <td class="xl631320" align="right">29.07.2009</td> | + | <td class="xl631320" align="right"><small>29.07.2009</small></td> |
- | <td class="xl641320" align="right">13:24</td> | + | <td class="xl641320" align="right"><small>13:24</small></td> |
- | <td class="xl151320" align="right">15.73</td> | + | <td class="xl151320" align="right"><small>15.73</small></td> |
- | <td class="xl151320" align="right">0.315</td> | + | <td class="xl151320" align="right"><small>0.315</small></td> |
- | <td class="xl151320" align="right">0.219</td> | + | <td class="xl151320" align="right"><small>0.219</small></td> |
- | <td class="xl151320" align="right">1.44</td> | + | <td class="xl151320" align="right"><small>1.44</small></td> |
- | <td class="xl151320" align="right">0.8</td> | + | <td class="xl151320" align="right"><small>0.8</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl151320" style="height: 15pt;" | | <td class="xl151320" style="height: 15pt;" |
- | height="20">Strep+Dig clone 4</td> | + | height="20"><small>Strep+Dig clone 4</small></td> |
- | <td class="xl151320">FreiGEM</td> | + | <td class="xl151320"><small>FreiGEM</small></td> |
- | <td class="xl631320" align="right">29.07.2009</td> | + | <td class="xl631320" align="right"><small>29.07.2009</small></td> |
- | <td class="xl641320" align="right">13:25</td> | + | <td class="xl641320" align="right"><small>13:25</small></td> |
- | <td class="xl151320" align="right">84.54</td> | + | <td class="xl151320" align="right"><small>84.54</small></td> |
- | <td class="xl151320" align="right">1.691</td> | + | <td class="xl151320" align="right"><small>1.691</small></td> |
- | <td class="xl151320" align="right">1.126</td> | + | <td class="xl151320" align="right"><small>1.126</small></td> |
- | <td class="xl151320" align="right">1.5</td> | + | <td class="xl151320" align="right"><small>1.5</small></td> |
- | <td class="xl151320" align="right">0.62</td> | + | <td class="xl151320" align="right"><small>0.62</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl151320" style="height: 15pt;" | | <td class="xl151320" style="height: 15pt;" |
- | height="20">His+Dig clone 3</td> | + | height="20"><small>His+Dig clone 3</small></td> |
- | <td class="xl151320">FreiGEM</td> | + | <td class="xl151320"><small>FreiGEM</small></td> |
- | <td class="xl631320" align="right">29.07.2009</td> | + | <td class="xl631320" align="right"><small>29.07.2009</small></td> |
- | <td class="xl641320" align="right">13:25</td> | + | <td class="xl641320" align="right"><small>13:25</small></td> |
- | <td class="xl151320" align="right">7.6</td> | + | <td class="xl151320" align="right"><small>7.6</small></td> |
- | <td class="xl151320" align="right">0.152</td> | + | <td class="xl151320" align="right"><small>0.152</small></td> |
- | <td class="xl151320" align="right">0.096</td> | + | <td class="xl151320" align="right"><small>0.096</small></td> |
- | <td class="xl151320" align="right">1.59</td> | + | <td class="xl151320" align="right"><small>1.59</small></td> |
- | <td class="xl151320" align="right">1.43</td> | + | <td class="xl151320" align="right"><small>1.43</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl151320" style="height: 15pt;" | | <td class="xl151320" style="height: 15pt;" |
- | height="20">His+Dig clone 3</td> | + | height="20"><small>His+Dig clone 3</small></td> |
- | <td class="xl151320">FreiGEM</td> | + | <td class="xl151320"><small>FreiGEM</small></td> |
- | <td class="xl631320" align="right">29.07.2009</td> | + | <td class="xl631320" align="right"><small>29.07.2009</small></td> |
- | <td class="xl641320" align="right">13:26</td> | + | <td class="xl641320" align="right"><small>13:26</small></td> |
- | <td class="xl151320" align="right">5.59</td> | + | <td class="xl151320" align="right"><small>5.59</small></td> |
- | <td class="xl151320" align="right">0.112</td> | + | <td class="xl151320" align="right"><small>0.112</small></td> |
- | <td class="xl151320" align="right">0.078</td> | + | <td class="xl151320" align="right"><small>0.078</small></td> |
- | <td class="xl151320" align="right">1.44</td> | + | <td class="xl151320" align="right"><small>1.44</small></td> |
- | <td class="xl151320" align="right">2.24</td> | + | <td class="xl151320" align="right"><small>2.24</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl151320" style="height: 15pt;" | | <td class="xl151320" style="height: 15pt;" |
- | height="20">His+Dig clone 3_2</td> | + | height="20"><small>His+Dig clone 3_2</small></td> |
- | <td class="xl151320">FreiGEM</td> | + | <td class="xl151320"><small>FreiGEM</small></td> |
- | <td class="xl631320" align="right">29.07.2009</td> | + | <td class="xl631320" align="right"><small>29.07.2009</small></td> |
- | <td class="xl641320" align="right">13:27</td> | + | <td class="xl641320" align="right"><small>13:27</small></td> |
- | <td class="xl151320" align="right">12.41</td> | + | <td class="xl151320" align="right"><small>12.41</small></td> |
- | <td class="xl151320" align="right">0.248</td> | + | <td class="xl151320" align="right"><small>0.248</small></td> |
- | <td class="xl151320" align="right">0.173</td> | + | <td class="xl151320" align="right"><small>0.173</small></td> |
- | <td class="xl151320" align="right">1.44</td> | + | <td class="xl151320" align="right"><small>1.44</small></td> |
- | <td class="xl151320" align="right">0.82</td> | + | <td class="xl151320" align="right"><small>0.82</small></td> |
| </tr> | | </tr> |
| <tr style="display: none;" height="0"> | | <tr style="display: none;" height="0"> |
Line 3,396: |
Line 3,293: |
| <ul> | | <ul> |
| <li> plasmidprep Dig+His, Dig+Strep, FluA+His, | | <li> plasmidprep Dig+His, Dig+Strep, FluA+His, |
- | FluA+Strep and Foki+Split | + | FluA+Strep and Foki+Split </li> |
- | </li>
| + | |
| <li> glycerol stock of Dig+His, Dig+Strep, FluA+His, | | <li> glycerol stock of Dig+His, Dig+Strep, FluA+His, |
- | FluA+Strep and Foki+Split | + | FluA+Strep and Foki+Split </li> |
- | </li>
| + | |
| <li> protein purification of Aa protein with Ni-NTA | | <li> protein purification of Aa protein with Ni-NTA |
| column | | column |
Line 3,417: |
Line 3,312: |
| <ul> | | <ul> |
| <li> over night-ligation of Dig+His_clon5, | | <li> over night-ligation of Dig+His_clon5, |
- | Dig+Strep_clon5, FluA+His_clon6, FluA+Strep_clon5 into pMAL | + | Dig+Strep_clon5, FluA+His_clon6, FluA+Strep_clon5 into pMAL </li> |
- | </li>
| + | <li> transformation of pMAL+CAT into Xblue </li> |
- | <li> transformation of pMAL+CAT into Xblue | + | |
- | </li>
| + | |
| <li><b>Nanodrop data</b></li> | | <li><b>Nanodrop data</b></li> |
| </ul> | | </ul> |
Line 3,430: |
Line 3,323: |
| <tr> | | <tr> |
| <td class="xl6515682" style="height: 15pt; width: 99pt;" | | <td class="xl6515682" style="height: 15pt; width: 99pt;" |
- | height="20" width="132">Sample | + | height="20" width="132"><small>Sample |
- | ID</td> | + | ID</small></td> |
| <td class="xl6515682" style="width: 48pt;" | | <td class="xl6515682" style="width: 48pt;" |
- | width="64">User ID</td> | + | width="64"><small>User ID</small></td> |
| <td class="xl6515682" style="width: 53pt;" | | <td class="xl6515682" style="width: 53pt;" |
- | width="71">Date<span style=""> </span></td> | + | width="71"><small>Date<span style=""> </span></small></td> |
| <td class="xl6515682" style="width: 48pt;" | | <td class="xl6515682" style="width: 48pt;" |
- | width="64">Time<span style=""> </span></td> | + | width="64"><small>Time<span style=""> </span></small></td> |
| <td class="xl6515682" style="width: 48pt;" | | <td class="xl6515682" style="width: 48pt;" |
- | width="64">ng/ul<span style=""> </span></td> | + | width="64"><small>ng/ul<span style=""> </span></small></td> |
| <td class="xl6515682" style="width: 48pt;" | | <td class="xl6515682" style="width: 48pt;" |
- | width="64">A260<span style=""> </span></td> | + | width="64"><small>A260<span style=""> </span></small></td> |
| <td class="xl6515682" style="width: 66pt;" | | <td class="xl6515682" style="width: 66pt;" |
- | width="88">A280<span style=""> </span></td> | + | width="88"><small>A280<span style=""> </span></small></td> |
| <td class="xl6515682" style="width: 71pt;" | | <td class="xl6515682" style="width: 71pt;" |
- | width="94">260/280<span style=""> </span></td> | + | width="94"><small>260/280<span style=""> </span></small></td> |
| <td class="xl6515682" style="width: 65pt;" | | <td class="xl6515682" style="width: 65pt;" |
- | width="87">260/230<span style=""> </span></td> | + | width="87"><small>260/230<span style=""> </span></small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl1515682" style="height: 15pt;" | | <td class="xl1515682" style="height: 15pt;" |
- | height="20">Foki+Split_clon5</td> | + | height="20"><small>Foki+Split_clon5</small></td> |
- | <td class="xl1515682">FreiGEM</td> | + | <td class="xl1515682"><small>FreiGEM</small></td> |
- | <td class="xl6315682" align="right">30.07.2009</td> | + | <td class="xl6315682" align="right"><small>30.07.2009</small></td> |
- | <td class="xl6415682" align="right">11:42</td> | + | <td class="xl6415682" align="right"><small>11:42</small></td> |
- | <td class="xl1515682" align="right">375.73</td> | + | <td class="xl1515682" align="right"><small>375.73</small></td> |
- | <td class="xl1515682" align="right">7.515</td> | + | <td class="xl1515682" align="right"><small>7.515</small></td> |
- | <td class="xl1515682" align="right">3.901</td> | + | <td class="xl1515682" align="right"><small>3.901</small></td> |
- | <td class="xl1515682" align="right">1.93</td> | + | <td class="xl1515682" align="right"><small>1.93</small></td> |
- | <td class="xl1515682" align="right">1.93</td> | + | <td class="xl1515682" align="right"><small>1.93</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl1515682" style="height: 15pt;" | | <td class="xl1515682" style="height: 15pt;" |
- | height="20">FokI+Split clon_6</td> | + | height="20"><small>FokI+Split clon_6</small></td> |
- | <td class="xl1515682">FreiGEM</td> | + | <td class="xl1515682"><small>FreiGEM</small></td> |
- | <td class="xl6315682" align="right">30.07.2009</td> | + | <td class="xl6315682" align="right"><small>30.07.2009</small></td> |
- | <td class="xl6415682" align="right">11:43</td> | + | <td class="xl6415682" align="right"><small>11:43</small></td> |
- | <td class="xl1515682" align="right">265.38</td> | + | <td class="xl1515682" align="right"><small>265.38</small></td> |
- | <td class="xl1515682" align="right">5.308</td> | + | <td class="xl1515682" align="right"><small>5.308</small></td> |
- | <td class="xl1515682" align="right">2.755</td> | + | <td class="xl1515682" align="right"><small>2.755</small></td> |
- | <td class="xl1515682" align="right">1.93</td> | + | <td class="xl1515682" align="right"><small>1.93</small></td> |
- | <td class="xl1515682" align="right">2.18</td> | + | <td class="xl1515682" align="right"><small>2.18</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl1515682" style="height: 15pt;" | | <td class="xl1515682" style="height: 15pt;" |
- | height="20">His+Lipo clon_5</td> | + | height="20"><small>His+Lipo clon_5</small></td> |
- | <td class="xl1515682">FreiGEM</td> | + | <td class="xl1515682"><small>FreiGEM</small></td> |
- | <td class="xl6315682" align="right">30.07.2009</td> | + | <td class="xl6315682" align="right"><small>30.07.2009</small></td> |
- | <td class="xl6415682" align="right">11:44</td> | + | <td class="xl6415682" align="right"><small>11:44</small></td> |
- | <td class="xl1515682" align="right">304.81</td> | + | <td class="xl1515682" align="right"><small>304.81</small></td> |
- | <td class="xl1515682" align="right">6.096</td> | + | <td class="xl1515682" align="right"><small>6.096</small></td> |
- | <td class="xl1515682" align="right">3.187</td> | + | <td class="xl1515682" align="right"><small>3.187</small></td> |
- | <td class="xl1515682" align="right">1.91</td> | + | <td class="xl1515682" align="right"><small>1.91</small></td> |
- | <td class="xl1515682" align="right">2.04</td> | + | <td class="xl1515682" align="right"><small>2.04</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl1515682" style="height: 15pt;" | | <td class="xl1515682" style="height: 15pt;" |
- | height="20">His+Lipo clon_6</td> | + | height="20"><small>His+Lipo clon_6</small></td> |
- | <td class="xl1515682">FreiGEM</td> | + | <td class="xl1515682"><small>FreiGEM</small></td> |
- | <td class="xl6315682" align="right">30.07.2009</td> | + | <td class="xl6315682" align="right"><small>30.07.2009</small></td> |
- | <td class="xl6415682" align="right">11:46</td> | + | <td class="xl6415682" align="right"><small>11:46</small></td> |
- | <td class="xl1515682" align="right">320.33</td> | + | <td class="xl1515682" align="right"><small>320.33</small></td> |
- | <td class="xl1515682" align="right">6.407</td> | + | <td class="xl1515682" align="right"><small>6.407</small></td> |
- | <td class="xl1515682" align="right">3.344</td> | + | <td class="xl1515682" align="right"><small>3.344</small></td> |
- | <td class="xl1515682" align="right">1.92</td> | + | <td class="xl1515682" align="right"><small>1.92</small></td> |
- | <td class="xl1515682" align="right">2.21</td> | + | <td class="xl1515682" align="right"><small>2.21</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl1515682" style="height: 15pt;" | | <td class="xl1515682" style="height: 15pt;" |
- | height="20">His+Dig clon_5</td> | + | height="20"><small>His+Dig clon_5</small></td> |
- | <td class="xl1515682">FreiGEM</td> | + | <td class="xl1515682"><small>FreiGEM</small></td> |
- | <td class="xl6315682" align="right">30.07.2009</td> | + | <td class="xl6315682" align="right"><small>30.07.2009</small></td> |
- | <td class="xl6415682" align="right">11:47</td> | + | <td class="xl6415682" align="right"><small>11:47</small></td> |
- | <td class="xl1515682" align="right">356.84</td> | + | <td class="xl1515682" align="right"><small>356.84</small></td> |
- | <td class="xl1515682" align="right">7.137</td> | + | <td class="xl1515682" align="right"><small>7.137</small></td> |
- | <td class="xl1515682" align="right">3.729</td> | + | <td class="xl1515682" align="right"><small>3.729</small></td> |
- | <td class="xl1515682" align="right">1.91</td> | + | <td class="xl1515682" align="right"><small>1.91</small></td> |
- | <td class="xl1515682" align="right">2.07</td> | + | <td class="xl1515682" align="right"><small>2.07</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl1515682" style="height: 15pt;" | | <td class="xl1515682" style="height: 15pt;" |
- | height="20">His+Dig clon_6</td> | + | height="20"><small>His+Dig clon_6</small></td> |
- | <td class="xl1515682">FreiGEM</td> | + | <td class="xl1515682"><small>FreiGEM</small></td> |
- | <td class="xl6315682" align="right">30.07.2009</td> | + | <td class="xl6315682" align="right"><small>30.07.2009</small></td> |
- | <td class="xl6415682" align="right">11:48</td> | + | <td class="xl6415682" align="right"><small>11:48</small></td> |
- | <td class="xl1515682" align="right">325.28</td> | + | <td class="xl1515682" align="right"><small>325.28</small></td> |
- | <td class="xl1515682" align="right">6.506</td> | + | <td class="xl1515682" align="right"><small>6.506</small></td> |
- | <td class="xl1515682" align="right">3.396</td> | + | <td class="xl1515682" align="right"><small>3.396</small></td> |
- | <td class="xl1515682" align="right">1.92</td> | + | <td class="xl1515682" align="right"><small>1.92</small></td> |
- | <td class="xl1515682" align="right">2.18</td> | + | <td class="xl1515682" align="right"><small>2.18</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl1515682" style="height: 15pt;" | | <td class="xl1515682" style="height: 15pt;" |
- | height="20">Strep+Dig clon_5</td> | + | height="20"><small>Strep+Dig clon_5</small></td> |
- | <td class="xl1515682">FreiGEM</td> | + | <td class="xl1515682"><small>FreiGEM</small></td> |
- | <td class="xl6315682" align="right">30.07.2009</td> | + | <td class="xl6315682" align="right"><small>30.07.2009</small></td> |
- | <td class="xl6415682" align="right">11:49</td> | + | <td class="xl6415682" align="right"><small>11:49</small></td> |
- | <td class="xl1515682" align="right">393.14</td> | + | <td class="xl1515682" align="right"><small>393.14</small></td> |
- | <td class="xl1515682" align="right">7.863</td> | + | <td class="xl1515682" align="right"><small>7.863</small></td> |
- | <td class="xl1515682" align="right">4.162</td> | + | <td class="xl1515682" align="right"><small>4.162</small></td> |
- | <td class="xl1515682" align="right">1.89</td> | + | <td class="xl1515682" align="right"><small>1.89</small></td> |
- | <td class="xl1515682" align="right">1.97</td> | + | <td class="xl1515682" align="right"><small>1.97</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl1515682" style="height: 15pt;" | | <td class="xl1515682" style="height: 15pt;" |
- | height="20">Strep+Dig clon_6</td> | + | height="20"><small>Strep+Dig clon_6</small></td> |
- | <td class="xl1515682">FreiGEM</td> | + | <td class="xl1515682"><small>FreiGEM</small></td> |
- | <td class="xl6315682" align="right">30.07.2009</td> | + | <td class="xl6315682" align="right"><small>30.07.2009</small></td> |
- | <td class="xl6415682" align="right">11:50</td> | + | <td class="xl6415682" align="right"><small>11:50</small></td> |
- | <td class="xl1515682" align="right">336.97</td> | + | <td class="xl1515682" align="right"><small>336.97</small></td> |
- | <td class="xl1515682" align="right">6.739</td> | + | <td class="xl1515682" align="right"><small>6.739</small></td> |
- | <td class="xl1515682" align="right">3.544</td> | + | <td class="xl1515682" align="right"><small>3.544</small></td> |
- | <td class="xl1515682" align="right">1.9</td> | + | <td class="xl1515682" align="right"><small>1.9</small></td> |
- | <td class="xl1515682" align="right">1.95</td> | + | <td class="xl1515682" align="right"><small>1.95</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl1515682" style="height: 15pt;" | | <td class="xl1515682" style="height: 15pt;" |
- | height="20">Strep+FluA clon_5</td> | + | height="20"><small>Strep+FluA clon_5</small></td> |
- | <td class="xl1515682">FreiGEM</td> | + | <td class="xl1515682"><small>FreiGEM</small></td> |
- | <td class="xl6315682" align="right">30.07.2009</td> | + | <td class="xl6315682" align="right"><small>30.07.2009</small></td> |
- | <td class="xl6415682" align="right">11:51</td> | + | <td class="xl6415682" align="right"><small>11:51</small></td> |
- | <td class="xl1515682" align="right">359</td> | + | <td class="xl1515682" align="right"><small>359</small></td> |
- | <td class="xl1515682" align="right">7.18</td> | + | <td class="xl1515682" align="right"><small>7.18</small></td> |
- | <td class="xl1515682" align="right">3.767</td> | + | <td class="xl1515682" align="right"><small>3.767</small></td> |
- | <td class="xl1515682" align="right">1.91</td> | + | <td class="xl1515682" align="right"><small>1.91</small></td> |
- | <td class="xl1515682" align="right">2.1</td> | + | <td class="xl1515682" align="right"><small>2.1</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl1515682" style="height: 15pt;" | | <td class="xl1515682" style="height: 15pt;" |
- | height="20">Strep+FluA clon_6</td> | + | height="20"><small>Strep+FluA clon_6</small></td> |
- | <td class="xl1515682">FreiGEM</td> | + | <td class="xl1515682"><small>FreiGEM</small></td> |
- | <td class="xl6315682" align="right">30.07.2009</td> | + | <td class="xl6315682" align="right"><small>30.07.2009</small></td> |
- | <td class="xl6415682" align="right">11:52</td> | + | <td class="xl6415682" align="right"><small>11:52</small></td> |
- | <td class="xl1515682" align="right">410.89</td> | + | <td class="xl1515682" align="right"><small>410.89</small></td> |
- | <td class="xl1515682" align="right">8.218</td> | + | <td class="xl1515682" align="right"><small>8.218</small></td> |
- | <td class="xl1515682" align="right">4.338</td> | + | <td class="xl1515682" align="right"><small>4.338</small></td> |
- | <td class="xl1515682" align="right">1.89</td> | + | <td class="xl1515682" align="right"><small>1.89</small></td> |
- | <td class="xl1515682" align="right">1.95</td> | + | <td class="xl1515682" align="right"><small>1.95</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl1515682" style="height: 15pt;" | | <td class="xl1515682" style="height: 15pt;" |
- | height="20">His/Dig</td> | + | height="20"><small>His/Dig</small></td> |
- | <td class="xl1515682">FreiGEM</td> | + | <td class="xl1515682"><small>FreiGEM</small></td> |
- | <td class="xl6315682" align="right">30.07.2009</td> | + | <td class="xl6315682" align="right"><small>30.07.2009</small></td> |
- | <td class="xl6415682" align="right">18:39</td> | + | <td class="xl6415682" align="right"><small>18:39</small></td> |
- | <td class="xl1515682" align="right">21.87</td> | + | <td class="xl1515682" align="right"><small>21.87</small></td> |
- | <td class="xl1515682" align="right">0.437</td> | + | <td class="xl1515682" align="right"><small>0.437</small></td> |
- | <td class="xl1515682" align="right">0.242</td> | + | <td class="xl1515682" align="right"><small>0.242</small></td> |
- | <td class="xl1515682" align="right">1.81</td> | + | <td class="xl1515682" align="right"><small>1.81</small></td> |
- | <td class="xl1515682" align="right">0.04</td> | + | <td class="xl1515682" align="right"><small>0.04</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl1515682" style="height: 15pt;" | | <td class="xl1515682" style="height: 15pt;" |
- | height="20">His/Dig_2.Messung</td> | + | height="20"><small>His/Dig_2.Messung</small></td> |
- | <td class="xl1515682">FreiGEM</td> | + | <td class="xl1515682"><small>FreiGEM</small></td> |
- | <td class="xl6315682" align="right">30.07.2009</td> | + | <td class="xl6315682" align="right"><small>30.07.2009</small></td> |
- | <td class="xl6415682" align="right">18:40</td> | + | <td class="xl6415682" align="right"><small>18:40</small></td> |
- | <td class="xl1515682" align="right">11.49</td> | + | <td class="xl1515682" align="right"><small>11.49</small></td> |
- | <td class="xl1515682" align="right">0.23</td> | + | <td class="xl1515682" align="right"><small>0.23</small></td> |
- | <td class="xl1515682" align="right">0.119</td> | + | <td class="xl1515682" align="right"><small>0.119</small></td> |
- | <td class="xl1515682" align="right">1.94</td> | + | <td class="xl1515682" align="right"><small>1.94</small></td> |
- | <td class="xl1515682" align="right">0.02</td> | + | <td class="xl1515682" align="right"><small>0.02</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl1515682" style="height: 15pt;" | | <td class="xl1515682" style="height: 15pt;" |
- | height="20">His/FluA</td> | + | height="20"><small>His/FluA</small></td> |
- | <td class="xl1515682">FreiGEM</td> | + | <td class="xl1515682"><small>FreiGEM</small></td> |
- | <td class="xl6315682" align="right">30.07.2009</td> | + | <td class="xl6315682" align="right"><small>30.07.2009</small></td> |
- | <td class="xl6415682" align="right">18:41</td> | + | <td class="xl6415682" align="right"><small>18:41</small></td> |
- | <td class="xl1515682" align="right">7.41</td> | + | <td class="xl1515682" align="right"><small>7.41</small></td> |
- | <td class="xl1515682" align="right">0.148</td> | + | <td class="xl1515682" align="right"><small>0.148</small></td> |
- | <td class="xl1515682" align="right">0.088</td> | + | <td class="xl1515682" align="right"><small>0.088</small></td> |
- | <td class="xl1515682" align="right">1.68</td> | + | <td class="xl1515682" align="right"><small>1.68</small></td> |
- | <td class="xl1515682" align="right">0.05</td> | + | <td class="xl1515682" align="right"><small>0.05</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl1515682" style="height: 15pt;" | | <td class="xl1515682" style="height: 15pt;" |
- | height="20">His/FluA_2.Messung</td> | + | height="20"><small>His/FluA_2.Messung</small></td> |
- | <td class="xl1515682">FreiGEM</td> | + | <td class="xl1515682"><small>FreiGEM</small></td> |
- | <td class="xl6315682" align="right">30.07.2009</td> | + | <td class="xl6315682" align="right"><small>30.07.2009</small></td> |
- | <td class="xl6415682" align="right">18:42</td> | + | <td class="xl6415682" align="right"><small>18:42</small></td> |
- | <td class="xl1515682" align="right">8.07</td> | + | <td class="xl1515682" align="right"><small>8.07</small></td> |
- | <td class="xl1515682" align="right">0.161</td> | + | <td class="xl1515682" align="right"><small>0.161</small></td> |
- | <td class="xl1515682" align="right">0.08</td> | + | <td class="xl1515682" align="right"><small>0.08</small></td> |
- | <td class="xl1515682" align="right">2.02</td> | + | <td class="xl1515682" align="right"><small>2.02</small></td> |
- | <td class="xl1515682" align="right">0.05</td> | + | <td class="xl1515682" align="right"><small>0.05</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl1515682" style="height: 15pt;" | | <td class="xl1515682" style="height: 15pt;" |
- | height="20">His_Dig_neu</td> | + | height="20"><small>His_Dig_neu</small></td> |
- | <td class="xl1515682">FreiGEM</td> | + | <td class="xl1515682"><small>FreiGEM</small></td> |
- | <td class="xl6315682" align="right">30.07.2009</td> | + | <td class="xl6315682" align="right"><small>30.07.2009</small></td> |
- | <td class="xl6415682" align="right">19:00</td> | + | <td class="xl6415682" align="right"><small>19:00</small></td> |
- | <td class="xl1515682" align="right">11.07</td> | + | <td class="xl1515682" align="right"><small>11.07</small></td> |
- | <td class="xl1515682" align="right">0.221</td> | + | <td class="xl1515682" align="right"><small>0.221</small></td> |
- | <td class="xl1515682" align="right">0.106</td> | + | <td class="xl1515682" align="right"><small>0.106</small></td> |
- | <td class="xl1515682" align="right">2.09</td> | + | <td class="xl1515682" align="right"><small>2.09</small></td> |
- | <td class="xl1515682" align="right">0.02</td> | + | <td class="xl1515682" align="right"><small>0.02</small></td> |
| </tr> | | </tr> |
| <tr style="display: none;" height="0"> | | <tr style="display: none;" height="0"> |
Line 3,648: |
Line 3,541: |
| <ul> | | <ul> |
| <li>Transformation of ligations His+Dig, Strep+Dig, His+FluA, | | <li>Transformation of ligations His+Dig, Strep+Dig, His+FluA, |
- | Strep+FluA in pMAL respectively (Isabel, Sarah) | + | Strep+FluA in pMAL respectively (Isabel, Sarah) </li> |
- | </li>
| + | |
| <li>generate an inventory of all stocks and plasmids in our | | <li>generate an inventory of all stocks and plasmids in our |
- | freezers (Isabel, Sarah, Gerrit) | + | freezers (Isabel, Sarah, Gerrit) </li> |
- | </li>
| + | <li>PCR with M13-DNA and GenIII-Primers (Max, Laura) </li> |
- | <li>PCR with M13-DNA and GenIII-Primers (Max, Laura) | + | |
- | </li>
| + | |
| <li>PCR with complementary linkers (short, middle and long) to | | <li>PCR with complementary linkers (short, middle and long) to |
- | hybridise(Max, Laura) | + | hybridise(Max, Laura) </li> |
- | </li>
| + | <li>SDS-Gel of purified Argonaut proteins (Hannes) </li> |
- | <li>SDS-Gel of purified Argonaut proteins (Hannes) | + | <li>scan gel pictures and upload them into wiki (Christoph) </li> |
- | </li>
| + | <li>order of new equipment like pipettes, racks etc (Timo) </li> |
- | <li>scan gel pictures and upload them into wiki (Christoph) | + | <li>analyse background activity of E. coli (Gerrit) </li> |
- | </li>
| + | <li>write the project abstract for official wiki (Max) </li> |
- | <li>order of new equipment like pipettes, racks etc (Timo) | + | <li>modelling and planing length of oligos(Rüdiger) </li> |
- | </li>
| + | |
- | <li>analyse background activity of E. coli (Gerrit) | + | |
- | </li>
| + | |
- | <li>write the project abstract for official wiki (Max) | + | |
- | </li>
| + | |
- | <li>modelling and planing length of oligos(Rüdiger) | + | |
- | </li>
| + | |
| </ul> | | </ul> |
| <b><br /> | | <b><br /> |
| Nanodrop data</b><br /> | | Nanodrop data</b><br /> |
| <ul> | | <ul> |
- | <li>Nucleic acid | + | <li>Nucleic acid </li> |
- | </li>
| + | |
| </ul> | | </ul> |
| <table | | <table |
Line 3,681: |
Line 3,563: |
| border="1" cellpadding="0" cellspacing="0" | | border="1" cellpadding="0" cellspacing="0" |
| width="682"> | | width="682"> |
- | <col span="2" style="width: 48pt;" width="64" /> | + | <col style="width: 48pt;" span="2" width="64" /> |
| <col style="width: 80pt;" width="106" /><col | | <col style="width: 80pt;" width="106" /><col |
- | span="7" style="width: 48pt;" width="64" /> | + | style="width: 48pt;" span="7" width="64" /> <tbody> |
- | <tbody>
| + | |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl151735" style="height: 15pt;" | | <td class="xl151735" style="height: 15pt;" |
- | height="20">Sample ID</td> | + | height="20"><small>Sample ID</small></td> |
- | <td class="xl641735">User ID</td> | + | <td class="xl641735"><small>User ID</small></td> |
- | <td class="xl641735" style="border-left: medium none;">Date</td> | + | <td class="xl641735" style="border-left: medium none;"><small>Date</small></td> |
- | <td class="xl641735" style="border-left: medium none;">Time</td> | + | <td class="xl641735" style="border-left: medium none;"><small>Time</small></td> |
- | <td class="xl641735" style="border-left: medium none;">ng/ul</td> | + | <td class="xl641735" style="border-left: medium none;"><small>ng/ul</small></td> |
- | <td class="xl641735" style="border-left: medium none;">A260</td> | + | <td class="xl641735" style="border-left: medium none;"><small>A260</small></td> |
- | <td class="xl641735" style="border-left: medium none;">A280</td> | + | <td class="xl641735" style="border-left: medium none;"><small>A280</small></td> |
- | <td class="xl641735" style="border-left: medium none;">260/280</td> | + | <td class="xl641735" style="border-left: medium none;"><small>260/280</small></td> |
- | <td class="xl641735" style="border-left: medium none;">260/230</td> | + | <td class="xl641735" style="border-left: medium none;"><small>260/230</small></td> |
- | <td class="xl641735" style="border-left: medium none;">Constant</td> | + | <td class="xl641735" style="border-left: medium none;"><small>Constant</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl151735" style="height: 15pt;" | | <td class="xl151735" style="height: 15pt;" |
- | height="20"></td> | + | height="20"><small></small></td> |
- | <td class="xl641735" style="border-top: medium none;"><span | + | <td class="xl641735" style="border-top: medium none;"><small><span |
- | style=""> </span>FreiGEM<span style=""> </span></td> | + | style=""> </span>FreiGEM<span style=""> </span></small></td> |
| <td class="xl641735" | | <td class="xl641735" |
- | style="border-top: medium none; border-left: medium none;"><span | + | style="border-top: medium none; border-left: medium none;"><small><span |
| style=""> </span>31.07.2009<span style=""> | | style=""> </span>31.07.2009<span style=""> |
- | </span></td> | + | </span></small></td> |
| <td class="xl641735" | | <td class="xl641735" |
- | style="border-top: medium none; border-left: medium none;"><span | + | style="border-top: medium none; border-left: medium none;"><small><span |
- | style=""> </span>17:53<span style=""> </span></td> | + | style=""> </span>17:53<span style=""> </span></small></td> |
| <td class="xl641735" | | <td class="xl641735" |
| style="border-top: medium none; border-left: medium none;" | | style="border-top: medium none; border-left: medium none;" |
- | align="right">0</td> | + | align="right"><small>0</small></td> |
| <td class="xl641735" | | <td class="xl641735" |
| style="border-top: medium none; border-left: medium none;" | | style="border-top: medium none; border-left: medium none;" |
- | align="right">0</td> | + | align="right"><small>0</small></td> |
| <td class="xl641735" | | <td class="xl641735" |
| style="border-top: medium none; border-left: medium none;" | | style="border-top: medium none; border-left: medium none;" |
- | align="right">0</td> | + | align="right"><small>0</small></td> |
| <td class="xl641735" | | <td class="xl641735" |
- | style="border-top: medium none; border-left: medium none;"><span | + | style="border-top: medium none; border-left: medium none;"><small><span |
- | style=""> </span>NaN<span style=""> </span></td> | + | style=""> </span>NaN<span style=""> </span></small></td> |
| <td class="xl641735" | | <td class="xl641735" |
- | style="border-top: medium none; border-left: medium none;"><span | + | style="border-top: medium none; border-left: medium none;"><small><span |
- | style=""> </span>NaN<span style=""> </span></td> | + | style=""> </span>NaN<span style=""> </span></small></td> |
| <td class="xl641735" | | <td class="xl641735" |
| style="border-top: medium none; border-left: medium none;" | | style="border-top: medium none; border-left: medium none;" |
- | align="right">50</td> | + | align="right"><small>50</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl151735" style="height: 15pt;" | | <td class="xl151735" style="height: 15pt;" |
- | height="20">GenIII_1</td> | + | height="20"><small>GenIII_1</small></td> |
- | <td class="xl641735" style="border-top: medium none;"><span | + | <td class="xl641735" style="border-top: medium none;"><small><span |
- | style=""> </span>FreiGEM<span style=""> </span></td> | + | style=""> </span>FreiGEM<span style=""> </span></small></td> |
| <td class="xl641735" | | <td class="xl641735" |
- | style="border-top: medium none; border-left: medium none;"><span | + | style="border-top: medium none; border-left: medium none;"><small><span |
| style=""> </span>31.07.2009<span style=""> | | style=""> </span>31.07.2009<span style=""> |
- | </span></td> | + | </span></small></td> |
| <td class="xl641735" | | <td class="xl641735" |
- | style="border-top: medium none; border-left: medium none;"><span | + | style="border-top: medium none; border-left: medium none;"><small><span |
- | style=""> </span>17:54<span style=""> </span></td> | + | style=""> </span>17:54<span style=""> </span></small></td> |
| <td class="xl641735" | | <td class="xl641735" |
| style="border-top: medium none; border-left: medium none;" | | style="border-top: medium none; border-left: medium none;" |
- | align="right">60.69</td> | + | align="right"><small>60.69</small></td> |
| <td class="xl641735" | | <td class="xl641735" |
| style="border-top: medium none; border-left: medium none;" | | style="border-top: medium none; border-left: medium none;" |
- | align="right">1.214</td> | + | align="right"><small>1.214</small></td> |
| <td class="xl641735" | | <td class="xl641735" |
| style="border-top: medium none; border-left: medium none;" | | style="border-top: medium none; border-left: medium none;" |
- | align="right">0.633</td> | + | align="right"><small>0.633</small></td> |
| <td class="xl641735" | | <td class="xl641735" |
| style="border-top: medium none; border-left: medium none;" | | style="border-top: medium none; border-left: medium none;" |
- | align="right">1.92</td> | + | align="right"><small>1.92</small></td> |
| <td class="xl641735" | | <td class="xl641735" |
| style="border-top: medium none; border-left: medium none;" | | style="border-top: medium none; border-left: medium none;" |
- | align="right">0.24</td> | + | align="right"><small>0.24</small></td> |
| <td class="xl641735" | | <td class="xl641735" |
| style="border-top: medium none; border-left: medium none;" | | style="border-top: medium none; border-left: medium none;" |
- | align="right">50</td> | + | align="right"><small>50</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl151735" style="height: 15pt;" | | <td class="xl151735" style="height: 15pt;" |
- | height="20">GenIII_1_<span style="display: none;">2.Messung</span></td> | + | height="20"><small>GenIII_1_<span |
- | <td class="xl641735" style="border-top: medium none;"><span | + | style="display: none;">2.Messung</span></small></td> |
- | style=""> </span>FreiGEM<span style=""> </span></td> | + | <td class="xl641735" style="border-top: medium none;"><small><span |
| + | style=""> </span>FreiGEM<span style=""> </span></small></td> |
| <td class="xl641735" | | <td class="xl641735" |
- | style="border-top: medium none; border-left: medium none;"><span | + | style="border-top: medium none; border-left: medium none;"><small><span |
| style=""> </span>31.07.2009<span style=""> | | style=""> </span>31.07.2009<span style=""> |
- | </span></td> | + | </span></small></td> |
| <td class="xl641735" | | <td class="xl641735" |
- | style="border-top: medium none; border-left: medium none;"><span | + | style="border-top: medium none; border-left: medium none;"><small><span |
- | style=""> </span>17:55<span style=""> </span></td> | + | style=""> </span>17:55<span style=""> </span></small></td> |
| <td class="xl641735" | | <td class="xl641735" |
| style="border-top: medium none; border-left: medium none;" | | style="border-top: medium none; border-left: medium none;" |
- | align="right">58.66</td> | + | align="right"><small>58.66</small></td> |
| <td class="xl641735" | | <td class="xl641735" |
| style="border-top: medium none; border-left: medium none;" | | style="border-top: medium none; border-left: medium none;" |
- | align="right">1.173</td> | + | align="right"><small>1.173</small></td> |
| <td class="xl641735" | | <td class="xl641735" |
| style="border-top: medium none; border-left: medium none;" | | style="border-top: medium none; border-left: medium none;" |
- | align="right">0.605</td> | + | align="right"><small>0.605</small></td> |
| <td class="xl641735" | | <td class="xl641735" |
| style="border-top: medium none; border-left: medium none;" | | style="border-top: medium none; border-left: medium none;" |
- | align="right">1.94</td> | + | align="right"><small>1.94</small></td> |
| <td class="xl641735" | | <td class="xl641735" |
| style="border-top: medium none; border-left: medium none;" | | style="border-top: medium none; border-left: medium none;" |
- | align="right">0.23</td> | + | align="right"><small>0.23</small></td> |
| <td class="xl641735" | | <td class="xl641735" |
| style="border-top: medium none; border-left: medium none;" | | style="border-top: medium none; border-left: medium none;" |
- | align="right">50</td> | + | align="right"><small>50</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl151735" style="height: 15pt;" | | <td class="xl151735" style="height: 15pt;" |
- | height="20">GenIII_2</td> | + | height="20"><small>GenIII_2</small></td> |
- | <td class="xl641735" style="border-top: medium none;"><span | + | <td class="xl641735" style="border-top: medium none;"><small><span |
- | style=""> </span>FreiGEM<span style=""> </span></td> | + | style=""> </span>FreiGEM<span style=""> </span></small></td> |
| <td class="xl641735" | | <td class="xl641735" |
- | style="border-top: medium none; border-left: medium none;"><span | + | style="border-top: medium none; border-left: medium none;"><small><span |
| style=""> </span>31.07.2009<span style=""> | | style=""> </span>31.07.2009<span style=""> |
- | </span></td> | + | </span></small></td> |
| <td class="xl641735" | | <td class="xl641735" |
- | style="border-top: medium none; border-left: medium none;"><span | + | style="border-top: medium none; border-left: medium none;"><small><span |
- | style=""> </span>17:56<span style=""> </span></td> | + | style=""> </span>17:56<span style=""> </span></small></td> |
| <td class="xl641735" | | <td class="xl641735" |
| style="border-top: medium none; border-left: medium none;" | | style="border-top: medium none; border-left: medium none;" |
- | align="right">65.85</td> | + | align="right"><small>65.85</small></td> |
| <td class="xl641735" | | <td class="xl641735" |
| style="border-top: medium none; border-left: medium none;" | | style="border-top: medium none; border-left: medium none;" |
- | align="right">1.317</td> | + | align="right"><small>1.317</small></td> |
| <td class="xl641735" | | <td class="xl641735" |
| style="border-top: medium none; border-left: medium none;" | | style="border-top: medium none; border-left: medium none;" |
- | align="right">0.676</td> | + | align="right"><small>0.676</small></td> |
| <td class="xl641735" | | <td class="xl641735" |
| style="border-top: medium none; border-left: medium none;" | | style="border-top: medium none; border-left: medium none;" |
- | align="right">1.95</td> | + | align="right"><small>1.95</small></td> |
| <td class="xl641735" | | <td class="xl641735" |
| style="border-top: medium none; border-left: medium none;" | | style="border-top: medium none; border-left: medium none;" |
- | align="right">0.13</td> | + | align="right"><small>0.13</small></td> |
| <td class="xl641735" | | <td class="xl641735" |
| style="border-top: medium none; border-left: medium none;" | | style="border-top: medium none; border-left: medium none;" |
- | align="right">50</td> | + | align="right"><small>50</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl151735" style="height: 15pt;" | | <td class="xl151735" style="height: 15pt;" |
- | height="20"></td> | + | height="20"><small></small></td> |
- | <td class="xl641735" style="border-top: medium none;"><span | + | <td class="xl641735" style="border-top: medium none;"><small><span |
- | style=""> </span>FreiGEM<span style=""> </span></td> | + | style=""> </span>FreiGEM<span style=""> </span></small></td> |
| <td class="xl641735" | | <td class="xl641735" |
- | style="border-top: medium none; border-left: medium none;"><span | + | style="border-top: medium none; border-left: medium none;"><small><span |
| style=""> </span>31.07.2009<span style=""> | | style=""> </span>31.07.2009<span style=""> |
- | </span></td> | + | </span></small></td> |
| <td class="xl641735" | | <td class="xl641735" |
- | style="border-top: medium none; border-left: medium none;"><span | + | style="border-top: medium none; border-left: medium none;"><small><span |
- | style=""> </span>18:14<span style=""> </span></td> | + | style=""> </span>18:14<span style=""> </span></small></td> |
| <td class="xl641735" | | <td class="xl641735" |
| style="border-top: medium none; border-left: medium none;" | | style="border-top: medium none; border-left: medium none;" |
- | align="right">0</td> | + | align="right"><small>0</small></td> |
| <td class="xl641735" | | <td class="xl641735" |
| style="border-top: medium none; border-left: medium none;" | | style="border-top: medium none; border-left: medium none;" |
- | align="right">0</td> | + | align="right"><small>0</small></td> |
| <td class="xl641735" | | <td class="xl641735" |
| style="border-top: medium none; border-left: medium none;" | | style="border-top: medium none; border-left: medium none;" |
- | align="right">0</td> | + | align="right"><small>0</small></td> |
| <td class="xl641735" | | <td class="xl641735" |
- | style="border-top: medium none; border-left: medium none;"><span | + | style="border-top: medium none; border-left: medium none;"><small><span |
- | style=""> </span>NaN<span style=""> </span></td> | + | style=""> </span>NaN<span style=""> </span></small></td> |
| <td class="xl641735" | | <td class="xl641735" |
- | style="border-top: medium none; border-left: medium none;"><span | + | style="border-top: medium none; border-left: medium none;"><small><span |
- | style=""> </span>NaN<span style=""> </span></td> | + | style=""> </span>NaN<span style=""> </span></small></td> |
| <td class="xl641735" | | <td class="xl641735" |
| style="border-top: medium none; border-left: medium none;" | | style="border-top: medium none; border-left: medium none;" |
- | align="right">50</td> | + | align="right"><small>50</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl151735" style="height: 15pt;" | | <td class="xl151735" style="height: 15pt;" |
- | height="20">Long_Link<span style="display: none;">er</span></td> | + | height="20"><small>Long_Link<span |
- | <td class="xl641735" style="border-top: medium none;"><span | + | style="display: none;">er</span></small></td> |
- | style=""> </span>FreiGEM<span style=""> </span></td> | + | <td class="xl641735" style="border-top: medium none;"><small><span |
| + | style=""> </span>FreiGEM<span style=""> </span></small></td> |
| <td class="xl641735" | | <td class="xl641735" |
- | style="border-top: medium none; border-left: medium none;"><span | + | style="border-top: medium none; border-left: medium none;"><small><span |
| style=""> </span>31.07.2009<span style=""> | | style=""> </span>31.07.2009<span style=""> |
- | </span></td> | + | </span></small></td> |
| <td class="xl641735" | | <td class="xl641735" |
- | style="border-top: medium none; border-left: medium none;"><span | + | style="border-top: medium none; border-left: medium none;"><small><span |
- | style=""> </span>18:16<span style=""> </span></td> | + | style=""> </span>18:16<span style=""> </span></small></td> |
| <td class="xl641735" | | <td class="xl641735" |
| style="border-top: medium none; border-left: medium none;" | | style="border-top: medium none; border-left: medium none;" |
- | align="right">1577.52</td> | + | align="right"><small>1577.52</small></td> |
| <td class="xl641735" | | <td class="xl641735" |
| style="border-top: medium none; border-left: medium none;" | | style="border-top: medium none; border-left: medium none;" |
- | align="right">31.55</td> | + | align="right"><small>31.55</small></td> |
| <td class="xl641735" | | <td class="xl641735" |
| style="border-top: medium none; border-left: medium none;" | | style="border-top: medium none; border-left: medium none;" |
- | align="right">16.931</td> | + | align="right"><small>16.931</small></td> |
| <td class="xl641735" | | <td class="xl641735" |
| style="border-top: medium none; border-left: medium none;" | | style="border-top: medium none; border-left: medium none;" |
- | align="right">1.86</td> | + | align="right"><small>1.86</small></td> |
| <td class="xl641735" | | <td class="xl641735" |
| style="border-top: medium none; border-left: medium none;" | | style="border-top: medium none; border-left: medium none;" |
- | align="right">2.02</td> | + | align="right"><small>2.02</small></td> |
| <td class="xl641735" | | <td class="xl641735" |
| style="border-top: medium none; border-left: medium none;" | | style="border-top: medium none; border-left: medium none;" |
- | align="right">50</td> | + | align="right"><small>50</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl151735" style="height: 15pt;" | | <td class="xl151735" style="height: 15pt;" |
- | height="20">Short_Lin<span style="display: none;">ker</span></td> | + | height="20"><small>Short_Lin<span |
- | <td class="xl641735" style="border-top: medium none;"><span | + | style="display: none;">ker</span></small></td> |
- | style=""> </span>FreiGEM<span style=""> </span></td> | + | <td class="xl641735" style="border-top: medium none;"><small><span |
| + | style=""> </span>FreiGEM<span style=""> </span></small></td> |
| <td class="xl641735" | | <td class="xl641735" |
- | style="border-top: medium none; border-left: medium none;"><span | + | style="border-top: medium none; border-left: medium none;"><small><span |
| style=""> </span>31.07.2009<span style=""> | | style=""> </span>31.07.2009<span style=""> |
- | </span></td> | + | </span></small></td> |
| <td class="xl641735" | | <td class="xl641735" |
- | style="border-top: medium none; border-left: medium none;"><span | + | style="border-top: medium none; border-left: medium none;"><small><span |
- | style=""> </span>18:17<span style=""> </span></td> | + | style=""> </span>18:17<span style=""> </span></small></td> |
| <td class="xl641735" | | <td class="xl641735" |
| style="border-top: medium none; border-left: medium none;" | | style="border-top: medium none; border-left: medium none;" |
- | align="right">2061.95</td> | + | align="right"><small>2061.95</small></td> |
| <td class="xl641735" | | <td class="xl641735" |
| style="border-top: medium none; border-left: medium none;" | | style="border-top: medium none; border-left: medium none;" |
- | align="right">41.239</td> | + | align="right"><small>41.239</small></td> |
| <td class="xl641735" | | <td class="xl641735" |
| style="border-top: medium none; border-left: medium none;" | | style="border-top: medium none; border-left: medium none;" |
- | align="right">22.11</td> | + | align="right"><small>22.11</small></td> |
| <td class="xl641735" | | <td class="xl641735" |
| style="border-top: medium none; border-left: medium none;" | | style="border-top: medium none; border-left: medium none;" |
- | align="right">1.87</td> | + | align="right"><small>1.87</small></td> |
| <td class="xl641735" | | <td class="xl641735" |
| style="border-top: medium none; border-left: medium none;" | | style="border-top: medium none; border-left: medium none;" |
- | align="right">2.03</td> | + | align="right"><small>2.03</small></td> |
| <td class="xl641735" | | <td class="xl641735" |
| style="border-top: medium none; border-left: medium none;" | | style="border-top: medium none; border-left: medium none;" |
- | align="right">50</td> | + | align="right"><small>50</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl151735" style="height: 15pt;" | | <td class="xl151735" style="height: 15pt;" |
- | height="20">Middle_li<span style="display: none;">nker</span></td> | + | height="20"><small>Middle_li<span |
- | <td class="xl641735" style="border-top: medium none;"><span | + | style="display: none;">nker</span></small></td> |
- | style=""> </span>FreiGEM<span style=""> </span></td> | + | <td class="xl641735" style="border-top: medium none;"><small><span |
| + | style=""> </span>FreiGEM<span style=""> </span></small></td> |
| <td class="xl641735" | | <td class="xl641735" |
- | style="border-top: medium none; border-left: medium none;"><span | + | style="border-top: medium none; border-left: medium none;"><small><span |
| style=""> </span>31.07.2009<span style=""> | | style=""> </span>31.07.2009<span style=""> |
- | </span></td> | + | </span></small></td> |
| <td class="xl641735" | | <td class="xl641735" |
- | style="border-top: medium none; border-left: medium none;"><span | + | style="border-top: medium none; border-left: medium none;"><small><span |
- | style=""> </span>18:18<span style=""> </span></td> | + | style=""> </span>18:18<span style=""> </span></small></td> |
| <td class="xl641735" | | <td class="xl641735" |
| style="border-top: medium none; border-left: medium none;" | | style="border-top: medium none; border-left: medium none;" |
- | align="right">1678.71</td> | + | align="right"><small>1678.71</small></td> |
| <td class="xl641735" | | <td class="xl641735" |
| style="border-top: medium none; border-left: medium none;" | | style="border-top: medium none; border-left: medium none;" |
- | align="right">33.574</td> | + | align="right"><small>33.574</small></td> |
| <td class="xl641735" | | <td class="xl641735" |
| style="border-top: medium none; border-left: medium none;" | | style="border-top: medium none; border-left: medium none;" |
- | align="right">18.1</td> | + | align="right"><small>18.1</small></td> |
| <td class="xl641735" | | <td class="xl641735" |
| style="border-top: medium none; border-left: medium none;" | | style="border-top: medium none; border-left: medium none;" |
- | align="right">1.85</td> | + | align="right"><small>1.85</small></td> |
| <td class="xl641735" | | <td class="xl641735" |
| style="border-top: medium none; border-left: medium none;" | | style="border-top: medium none; border-left: medium none;" |
- | align="right">2.01</td> | + | align="right"><small>2.01</small></td> |
| <td class="xl641735" | | <td class="xl641735" |
| style="border-top: medium none; border-left: medium none;" | | style="border-top: medium none; border-left: medium none;" |
- | align="right">50</td> | + | align="right"><small>50</small></td> |
| </tr> | | </tr> |
| <tr style="display: none;" height="0"> | | <tr style="display: none;" height="0"> |
Line 3,969: |
Line 3,854: |
| border="1" cellpadding="0" cellspacing="0" | | border="1" cellpadding="0" cellspacing="0" |
| width="595"> | | width="595"> |
- | <col style="width: 77pt;" width="103" /> | + | <col style="width: 77pt;" width="103" /> <col |
- | <col style="width: 48pt;" width="64" /><col
| + | style="width: 48pt;" width="64" /><col |
- | style="width: 65pt;" width="86" /> | + | style="width: 65pt;" width="86" /> <col |
- | <col span="3" style="width: 48pt;" width="64" /><col
| + | style="width: 48pt;" span="3" width="64" /><col |
- | style="width: 65pt;" width="86" /> | + | style="width: 65pt;" width="86" /> <col |
- | <col style="width: 48pt;" width="64" /><tbody>
| + | style="width: 48pt;" width="64" /><tbody> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl6314788" style="height: 15pt; width: 77pt;" | | <td class="xl6314788" style="height: 15pt; width: 77pt;" |
- | height="20" width="103">Sample | + | height="20" width="103"><small>Sample |
- | ID</td> | + | ID</small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-left: medium none; width: 48pt;" width="64">User | + | style="border-left: medium none; width: 48pt;" width="64"><small>User |
- | ID</td> | + | ID</small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-left: medium none; width: 65pt;" width="86">Date</td> | + | style="border-left: medium none; width: 65pt;" width="86"><small>Date</small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-left: medium none; width: 48pt;" width="64">Time</td> | + | style="border-left: medium none; width: 48pt;" width="64"><small>Time</small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-left: medium none; width: 48pt;" width="64">mg/ml</td> | + | style="border-left: medium none; width: 48pt;" width="64"><small>mg/ml</small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-left: medium none; width: 48pt;" width="64">260/280</td> | + | style="border-left: medium none; width: 48pt;" width="64"><small>260/280</small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-left: medium none; width: 65pt;" width="86">A280 | + | style="border-left: medium none; width: 65pt;" width="86"><small>A280 |
- | 10mm</td> | + | 10mm</small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-left: medium none; width: 48pt;" width="64">E | + | style="border-left: medium none; width: 48pt;" width="64"><small>E |
- | 1%</td> | + | 1%</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl6414788" | | <td class="xl6414788" |
- | style="border-top: medium none; height: 15pt;" height="20"> </td> | + | style="border-top: medium none; height: 15pt;" height="20"><small> </small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-top: medium none; border-left: medium none;"><span | + | style="border-top: medium none; border-left: medium none;"><small><span |
- | style=""> </span>FreiGEM<span style=""> </span></td> | + | style=""> </span>FreiGEM<span style=""> </span></small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-top: medium none; border-left: medium none;"><span | + | style="border-top: medium none; border-left: medium none;"><small><span |
| style=""> </span>31.07.2009<span style=""> | | style=""> </span>31.07.2009<span style=""> |
- | </span></td> | + | </span></small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-top: medium none; border-left: medium none;"><span | + | style="border-top: medium none; border-left: medium none;"><small><span |
- | style=""> </span>17:03<span style=""> </span></td> | + | style=""> </span>17:03<span style=""> </span></small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-top: medium none; border-left: medium none;">0</td> | + | style="border-top: medium none; border-left: medium none;"><small>0</small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-top: medium none; border-left: medium none;"><span | + | style="border-top: medium none; border-left: medium none;"><small><span |
- | style=""> </span>NaN<span style=""> </span></td> | + | style=""> </span>NaN<span style=""> </span></small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-top: medium none; border-left: medium none;">0</td> | + | style="border-top: medium none; border-left: medium none;"><small>0</small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-top: medium none; border-left: medium none;">10</td> | + | style="border-top: medium none; border-left: medium none;"><small>10</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl6414788" | | <td class="xl6414788" |
- | style="border-top: medium none; height: 15pt;" height="20">310709AaE1</td> | + | style="border-top: medium none; height: 15pt;" height="20"><small>310709AaE1</small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-top: medium none; border-left: medium none;"><span | + | style="border-top: medium none; border-left: medium none;"><small><span |
- | style=""> </span>FreiGEM<span style=""> </span></td> | + | style=""> </span>FreiGEM<span style=""> </span></small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-top: medium none; border-left: medium none;"><span | + | style="border-top: medium none; border-left: medium none;"><small><span |
| style=""> </span>31.07.2009<span style=""> | | style=""> </span>31.07.2009<span style=""> |
- | </span></td> | + | </span></small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-top: medium none; border-left: medium none;"><span | + | style="border-top: medium none; border-left: medium none;"><small><span |
- | style=""> </span>17:04<span style=""> </span></td> | + | style=""> </span>17:04<span style=""> </span></small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-top: medium none; border-left: medium none;">0.43</td> | + | style="border-top: medium none; border-left: medium none;"><small>0.43</small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-top: medium none; border-left: medium none;">2.08</td> | + | style="border-top: medium none; border-left: medium none;"><small>2.08</small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-top: medium none; border-left: medium none;">0.428</td> | + | style="border-top: medium none; border-left: medium none;"><small>0.428</small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-top: medium none; border-left: medium none;">10</td> | + | style="border-top: medium none; border-left: medium none;"><small>10</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl6414788" | | <td class="xl6414788" |
- | style="border-top: medium none; height: 15pt;" height="20">310709AaE2</td> | + | style="border-top: medium none; height: 15pt;" height="20"><small>310709AaE2</small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-top: medium none; border-left: medium none;"><span | + | style="border-top: medium none; border-left: medium none;"><small><span |
- | style=""> </span>FreiGEM<span style=""> </span></td> | + | style=""> </span>FreiGEM<span style=""> </span></small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-top: medium none; border-left: medium none;"><span | + | style="border-top: medium none; border-left: medium none;"><small><span |
| style=""> </span>31.07.2009<span style=""> | | style=""> </span>31.07.2009<span style=""> |
- | </span></td> | + | </span></small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-top: medium none; border-left: medium none;"><span | + | style="border-top: medium none; border-left: medium none;"><small><span |
- | style=""> </span>17:05<span style=""> </span></td> | + | style=""> </span>17:05<span style=""> </span></small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-top: medium none; border-left: medium none;">0.52</td> | + | style="border-top: medium none; border-left: medium none;"><small>0.52</small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-top: medium none; border-left: medium none;">1.12</td> | + | style="border-top: medium none; border-left: medium none;"><small>1.12</small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-top: medium none; border-left: medium none;">0.522</td> | + | style="border-top: medium none; border-left: medium none;"><small>0.522</small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-top: medium none; border-left: medium none;">10</td> | + | style="border-top: medium none; border-left: medium none;"><small>10</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl6414788" | | <td class="xl6414788" |
- | style="border-top: medium none; height: 15pt;" height="20">310709AaE1</td> | + | style="border-top: medium none; height: 15pt;" height="20"><small>310709AaE1</small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-top: medium none; border-left: medium none;"><span | + | style="border-top: medium none; border-left: medium none;"><small><span |
- | style=""> </span>FreiGEM<span style=""> </span></td> | + | style=""> </span>FreiGEM<span style=""> </span></small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-top: medium none; border-left: medium none;"><span | + | style="border-top: medium none; border-left: medium none;"><small><span |
| style=""> </span>31.07.2009<span style=""> | | style=""> </span>31.07.2009<span style=""> |
- | </span></td> | + | </span></small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-top: medium none; border-left: medium none;"><span | + | style="border-top: medium none; border-left: medium none;"><small><span |
- | style=""> </span>17:12<span style=""> </span></td> | + | style=""> </span>17:12<span style=""> </span></small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-top: medium none; border-left: medium none;">0.22</td> | + | style="border-top: medium none; border-left: medium none;"><small>0.22</small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-top: medium none; border-left: medium none;">2.34</td> | + | style="border-top: medium none; border-left: medium none;"><small>2.34</small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-top: medium none; border-left: medium none;">0.22</td> | + | style="border-top: medium none; border-left: medium none;"><small>0.22</small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-top: medium none; border-left: medium none;">10</td> | + | style="border-top: medium none; border-left: medium none;"><small>10</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl6414788" | | <td class="xl6414788" |
- | style="border-top: medium none; height: 15pt;" height="20">310709AaE3</td> | + | style="border-top: medium none; height: 15pt;" height="20"><small>310709AaE3</small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-top: medium none; border-left: medium none;"><span | + | style="border-top: medium none; border-left: medium none;"><small><span |
- | style=""> </span>FreiGEM<span style=""> </span></td> | + | style=""> </span>FreiGEM<span style=""> </span></small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-top: medium none; border-left: medium none;"><span | + | style="border-top: medium none; border-left: medium none;"><small><span |
| style=""> </span>31.07.2009<span style=""> | | style=""> </span>31.07.2009<span style=""> |
- | </span></td> | + | </span></small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-top: medium none; border-left: medium none;"><span | + | style="border-top: medium none; border-left: medium none;"><small><span |
- | style=""> </span>17:13<span style=""> </span></td> | + | style=""> </span>17:13<span style=""> </span></small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-top: medium none; border-left: medium none;">0.31</td> | + | style="border-top: medium none; border-left: medium none;"><small>0.31</small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-top: medium none; border-left: medium none;">0.83</td> | + | style="border-top: medium none; border-left: medium none;"><small>0.83</small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-top: medium none; border-left: medium none;">0.315</td> | + | style="border-top: medium none; border-left: medium none;"><small>0.315</small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-top: medium none; border-left: medium none;">10</td> | + | style="border-top: medium none; border-left: medium none;"><small>10</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl6414788" | | <td class="xl6414788" |
- | style="border-top: medium none; height: 15pt;" height="20">310709AaE4</td> | + | style="border-top: medium none; height: 15pt;" height="20"><small>310709AaE4</small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-top: medium none; border-left: medium none;"><span | + | style="border-top: medium none; border-left: medium none;"><small><span |
- | style=""> </span>FreiGEM<span style=""> </span></td> | + | style=""> </span>FreiGEM<span style=""> </span></small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-top: medium none; border-left: medium none;"><span | + | style="border-top: medium none; border-left: medium none;"><small><span |
| style=""> </span>31.07.2009<span style=""> | | style=""> </span>31.07.2009<span style=""> |
- | </span></td> | + | </span></small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-top: medium none; border-left: medium none;"><span | + | style="border-top: medium none; border-left: medium none;"><small><span |
- | style=""> </span>17:14<span style=""> </span></td> | + | style=""> </span>17:14<span style=""> </span></small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-top: medium none; border-left: medium none;">0.08</td> | + | style="border-top: medium none; border-left: medium none;"><small>0.08</small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-top: medium none; border-left: medium none;">2.03</td> | + | style="border-top: medium none; border-left: medium none;"><small>2.03</small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-top: medium none; border-left: medium none;">0.079</td> | + | style="border-top: medium none; border-left: medium none;"><small>0.079</small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-top: medium none; border-left: medium none;">10</td> | + | style="border-top: medium none; border-left: medium none;"><small>10</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl6414788" | | <td class="xl6414788" |
- | style="border-top: medium none; height: 15pt;" height="20">310709AaE5</td> | + | style="border-top: medium none; height: 15pt;" height="20"><small>310709AaE5</small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-top: medium none; border-left: medium none;"><span | + | style="border-top: medium none; border-left: medium none;"><small><span |
- | style=""> </span>FreiGEM<span style=""> </span></td> | + | style=""> </span>FreiGEM<span style=""> </span></small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-top: medium none; border-left: medium none;"><span | + | style="border-top: medium none; border-left: medium none;"><small><span |
| style=""> </span>31.07.2009<span style=""> | | style=""> </span>31.07.2009<span style=""> |
- | </span></td> | + | </span></small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-top: medium none; border-left: medium none;"><span | + | style="border-top: medium none; border-left: medium none;"><small><span |
- | style=""> </span>17:15<span style=""> </span></td> | + | style=""> </span>17:15<span style=""> </span></small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-top: medium none; border-left: medium none;">0.11</td> | + | style="border-top: medium none; border-left: medium none;"><small>0.11</small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-top: medium none; border-left: medium none;">1.55</td> | + | style="border-top: medium none; border-left: medium none;"><small>1.55</small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-top: medium none; border-left: medium none;">0.11</td> | + | style="border-top: medium none; border-left: medium none;"><small>0.11</small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-top: medium none; border-left: medium none;">10</td> | + | style="border-top: medium none; border-left: medium none;"><small>10</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl6414788" | | <td class="xl6414788" |
- | style="border-top: medium none; height: 15pt;" height="20">310709AaE4</td> | + | style="border-top: medium none; height: 15pt;" height="20"><small>310709AaE4</small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-top: medium none; border-left: medium none;"><span | + | style="border-top: medium none; border-left: medium none;"><small><span |
- | style=""> </span>FreiGEM<span style=""> </span></td> | + | style=""> </span>FreiGEM<span style=""> </span></small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-top: medium none; border-left: medium none;"><span | + | style="border-top: medium none; border-left: medium none;"><small><span |
| style=""> </span>31.07.2009<span style=""> | | style=""> </span>31.07.2009<span style=""> |
- | </span></td> | + | </span></small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-top: medium none; border-left: medium none;"><span | + | style="border-top: medium none; border-left: medium none;"><small><span |
- | style=""> </span>17:16<span style=""> </span></td> | + | style=""> </span>17:16<span style=""> </span></small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-top: medium none; border-left: medium none;">0.21</td> | + | style="border-top: medium none; border-left: medium none;"><small>0.21</small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-top: medium none; border-left: medium none;">1.22</td> | + | style="border-top: medium none; border-left: medium none;"><small>1.22</small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-top: medium none; border-left: medium none;">0.214</td> | + | style="border-top: medium none; border-left: medium none;"><small>0.214</small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-top: medium none; border-left: medium none;">10</td> | + | style="border-top: medium none; border-left: medium none;"><small>10</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl6414788" | | <td class="xl6414788" |
- | style="border-top: medium none; height: 15pt;" height="20">310709AaE6</td> | + | style="border-top: medium none; height: 15pt;" height="20"><small>310709AaE6</small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-top: medium none; border-left: medium none;"><span | + | style="border-top: medium none; border-left: medium none;"><small><span |
- | style=""> </span>FreiGEM<span style=""> </span></td> | + | style=""> </span>FreiGEM<span style=""> </span></small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-top: medium none; border-left: medium none;"><span | + | style="border-top: medium none; border-left: medium none;"><small><span |
| style=""> </span>31.07.2009<span style=""> | | style=""> </span>31.07.2009<span style=""> |
- | </span></td> | + | </span></small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-top: medium none; border-left: medium none;"><span | + | style="border-top: medium none; border-left: medium none;"><small><span |
- | style=""> </span>17:17<span style=""> </span></td> | + | style=""> </span>17:17<span style=""> </span></small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-top: medium none; border-left: medium none;">0.1</td> | + | style="border-top: medium none; border-left: medium none;"><small>0.1</small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-top: medium none; border-left: medium none;">1.65</td> | + | style="border-top: medium none; border-left: medium none;"><small>1.65</small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-top: medium none; border-left: medium none;">0.104</td> | + | style="border-top: medium none; border-left: medium none;"><small>0.104</small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-top: medium none; border-left: medium none;">10</td> | + | style="border-top: medium none; border-left: medium none;"><small>10</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl6414788" | | <td class="xl6414788" |
- | style="border-top: medium none; height: 15pt;" height="20">310709AaE7</td> | + | style="border-top: medium none; height: 15pt;" height="20"><small>310709AaE7</small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-top: medium none; border-left: medium none;"><span | + | style="border-top: medium none; border-left: medium none;"><small><span |
- | style=""> </span>FreiGEM<span style=""> </span></td> | + | style=""> </span>FreiGEM<span style=""> </span></small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-top: medium none; border-left: medium none;"><span | + | style="border-top: medium none; border-left: medium none;"><small><span |
| style=""> </span>31.07.2009<span style=""> | | style=""> </span>31.07.2009<span style=""> |
- | </span></td> | + | </span></small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-top: medium none; border-left: medium none;"><span | + | style="border-top: medium none; border-left: medium none;"><small><span |
- | style=""> </span>17:18<span style=""> </span></td> | + | style=""> </span>17:18<span style=""> </span></small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-top: medium none; border-left: medium none;">0.1</td> | + | style="border-top: medium none; border-left: medium none;"><small>0.1</small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-top: medium none; border-left: medium none;">1.56</td> | + | style="border-top: medium none; border-left: medium none;"><small>1.56</small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-top: medium none; border-left: medium none;">0.105</td> | + | style="border-top: medium none; border-left: medium none;"><small>0.105</small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-top: medium none; border-left: medium none;">10</td> | + | style="border-top: medium none; border-left: medium none;"><small>10</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl6414788" | | <td class="xl6414788" |
- | style="border-top: medium none; height: 15pt;" height="20">310709AaE8</td> | + | style="border-top: medium none; height: 15pt;" height="20"><small>310709AaE8</small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-top: medium none; border-left: medium none;"><span | + | style="border-top: medium none; border-left: medium none;"><small><span |
- | style=""> </span>FreiGEM<span style=""> </span></td> | + | style=""> </span>FreiGEM<span style=""> </span></small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-top: medium none; border-left: medium none;"><span | + | style="border-top: medium none; border-left: medium none;"><small><span |
| style=""> </span>31.07.2009<span style=""> | | style=""> </span>31.07.2009<span style=""> |
- | </span></td> | + | </span></small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-top: medium none; border-left: medium none;"><span | + | style="border-top: medium none; border-left: medium none;"><small><span |
- | style=""> </span>17:19<span style=""> </span></td> | + | style=""> </span>17:19<span style=""> </span></small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-top: medium none; border-left: medium none;">0.09</td> | + | style="border-top: medium none; border-left: medium none;"><small>0.09</small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-top: medium none; border-left: medium none;">1.6</td> | + | style="border-top: medium none; border-left: medium none;"><small>1.6</small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-top: medium none; border-left: medium none;">0.087</td> | + | style="border-top: medium none; border-left: medium none;"><small>0.087</small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-top: medium none; border-left: medium none;">10</td> | + | style="border-top: medium none; border-left: medium none;"><small>10</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl6414788" | | <td class="xl6414788" |
- | style="border-top: medium none; height: 15pt;" height="20">310709AaE9</td> | + | style="border-top: medium none; height: 15pt;" height="20"><small>310709AaE9</small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-top: medium none; border-left: medium none;"><span | + | style="border-top: medium none; border-left: medium none;"><small><span |
- | style=""> </span>FreiGEM<span style=""> </span></td> | + | style=""> </span>FreiGEM<span style=""> </span></small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-top: medium none; border-left: medium none;"><span | + | style="border-top: medium none; border-left: medium none;"><small><span |
| style=""> </span>31.07.2009<span style=""> | | style=""> </span>31.07.2009<span style=""> |
- | </span></td> | + | </span></small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-top: medium none; border-left: medium none;"><span | + | style="border-top: medium none; border-left: medium none;"><small><span |
- | style=""> </span>17:20<span style=""> </span></td> | + | style=""> </span>17:20<span style=""> </span></small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-top: medium none; border-left: medium none;">0.08</td> | + | style="border-top: medium none; border-left: medium none;"><small>0.08</small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-top: medium none; border-left: medium none;">1.69</td> | + | style="border-top: medium none; border-left: medium none;"><small>1.69</small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-top: medium none; border-left: medium none;">0.076</td> | + | style="border-top: medium none; border-left: medium none;"><small>0.076</small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-top: medium none; border-left: medium none;">10</td> | + | style="border-top: medium none; border-left: medium none;"><small>10</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl6414788" | | <td class="xl6414788" |
- | style="border-top: medium none; height: 15pt;" height="20">310709AaE10</td> | + | style="border-top: medium none; height: 15pt;" height="20"><small>310709AaE10</small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-top: medium none; border-left: medium none;"><span | + | style="border-top: medium none; border-left: medium none;"><small><span |
- | style=""> </span>FreiGEM<span style=""> </span></td> | + | style=""> </span>FreiGEM<span style=""> </span></small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-top: medium none; border-left: medium none;"><span | + | style="border-top: medium none; border-left: medium none;"><small><span |
| style=""> </span>31.07.2009<span style=""> | | style=""> </span>31.07.2009<span style=""> |
- | </span></td> | + | </span></small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-top: medium none; border-left: medium none;"><span | + | style="border-top: medium none; border-left: medium none;"><small><span |
- | style=""> </span>17:21<span style=""> </span></td> | + | style=""> </span>17:21<span style=""> </span></small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-top: medium none; border-left: medium none;">0.09</td> | + | style="border-top: medium none; border-left: medium none;"><small>0.09</small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-top: medium none; border-left: medium none;">1.33</td> | + | style="border-top: medium none; border-left: medium none;"><small>1.33</small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-top: medium none; border-left: medium none;">0.091</td> | + | style="border-top: medium none; border-left: medium none;"><small>0.091</small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-top: medium none; border-left: medium none;">10</td> | + | style="border-top: medium none; border-left: medium none;"><small>10</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl6414788" | | <td class="xl6414788" |
- | style="border-top: medium none; height: 15pt;" height="20">310709AaE10</td> | + | style="border-top: medium none; height: 15pt;" height="20"><small>310709AaE10</small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-top: medium none; border-left: medium none;"><span | + | style="border-top: medium none; border-left: medium none;"><small><span |
- | style=""> </span>FreiGEM<span style=""> </span></td> | + | style=""> </span>FreiGEM<span style=""> </span></small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-top: medium none; border-left: medium none;"><span | + | style="border-top: medium none; border-left: medium none;"><small><span |
| style=""> </span>31.07.2009<span style=""> | | style=""> </span>31.07.2009<span style=""> |
- | </span></td> | + | </span></small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-top: medium none; border-left: medium none;"><span | + | style="border-top: medium none; border-left: medium none;"><small><span |
- | style=""> </span>17:21<span style=""> </span></td> | + | style=""> </span>17:21<span style=""> </span></small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-top: medium none; border-left: medium none;">0.1</td> | + | style="border-top: medium none; border-left: medium none;"><small>0.1</small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-top: medium none; border-left: medium none;">1.34</td> | + | style="border-top: medium none; border-left: medium none;"><small>1.34</small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-top: medium none; border-left: medium none;">0.101</td> | + | style="border-top: medium none; border-left: medium none;"><small>0.101</small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-top: medium none; border-left: medium none;">10</td> | + | style="border-top: medium none; border-left: medium none;"><small>10</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl6414788" | | <td class="xl6414788" |
- | style="border-top: medium none; height: 15pt;" height="20">310709AaE11</td> | + | style="border-top: medium none; height: 15pt;" height="20"><small>310709AaE11</small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-top: medium none; border-left: medium none;"><span | + | style="border-top: medium none; border-left: medium none;"><small><span |
- | style=""> </span>FreiGEM<span style=""> </span></td> | + | style=""> </span>FreiGEM<span style=""> </span></small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-top: medium none; border-left: medium none;"><span | + | style="border-top: medium none; border-left: medium none;"><small><span |
| style=""> </span>31.07.2009<span style=""> | | style=""> </span>31.07.2009<span style=""> |
- | </span></td> | + | </span></small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-top: medium none; border-left: medium none;"><span | + | style="border-top: medium none; border-left: medium none;"><small><span |
- | style=""> </span>17:22<span style=""> </span></td> | + | style=""> </span>17:22<span style=""> </span></small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-top: medium none; border-left: medium none;">0.07</td> | + | style="border-top: medium none; border-left: medium none;"><small>0.07</small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-top: medium none; border-left: medium none;">1.88</td> | + | style="border-top: medium none; border-left: medium none;"><small>1.88</small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-top: medium none; border-left: medium none;">0.074</td> | + | style="border-top: medium none; border-left: medium none;"><small>0.074</small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-top: medium none; border-left: medium none;">10</td> | + | style="border-top: medium none; border-left: medium none;"><small>10</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl6414788" | | <td class="xl6414788" |
- | style="border-top: medium none; height: 15pt;" height="20">310709AaE12</td> | + | style="border-top: medium none; height: 15pt;" height="20"><small>310709AaE12</small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-top: medium none; border-left: medium none;"><span | + | style="border-top: medium none; border-left: medium none;"><small><span |
- | style=""> </span>FreiGEM<span style=""> </span></td> | + | style=""> </span>FreiGEM<span style=""> </span></small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-top: medium none; border-left: medium none;"><span | + | style="border-top: medium none; border-left: medium none;"><small><span |
| style=""> </span>31.07.2009<span style=""> | | style=""> </span>31.07.2009<span style=""> |
- | </span></td> | + | </span></small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-top: medium none; border-left: medium none;"><span | + | style="border-top: medium none; border-left: medium none;"><small><span |
- | style=""> </span>17:22<span style=""> </span></td> | + | style=""> </span>17:22<span style=""> </span></small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-top: medium none; border-left: medium none;">0.09</td> | + | style="border-top: medium none; border-left: medium none;"><small>0.09</small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-top: medium none; border-left: medium none;">1.1</td> | + | style="border-top: medium none; border-left: medium none;"><small>1.1</small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-top: medium none; border-left: medium none;">0.094</td> | + | style="border-top: medium none; border-left: medium none;"><small>0.094</small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-top: medium none; border-left: medium none;">10</td> | + | style="border-top: medium none; border-left: medium none;"><small>10</small></td> |
| </tr> | | </tr> |
| <tr style="height: 15pt;" height="20"> | | <tr style="height: 15pt;" height="20"> |
| <td class="xl6414788" | | <td class="xl6414788" |
- | style="border-top: medium none; height: 15pt;" height="20">310709AaE12</td> | + | style="border-top: medium none; height: 15pt;" height="20"><small>310709AaE12</small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-top: medium none; border-left: medium none;"><span | + | style="border-top: medium none; border-left: medium none;"><small><span |
- | style=""> </span>FreiGEM<span style=""> </span></td> | + | style=""> </span>FreiGEM<span style=""> </span></small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-top: medium none; border-left: medium none;"><span | + | style="border-top: medium none; border-left: medium none;"><small><span |
| style=""> </span>31.07.2009<span style=""> | | style=""> </span>31.07.2009<span style=""> |
- | </span></td> | + | </span></small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-top: medium none; border-left: medium none;"><span | + | style="border-top: medium none; border-left: medium none;"><small><span |
- | style=""> </span>17:23<span style=""> </span></td> | + | style=""> </span>17:23<span style=""> </span></small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-top: medium none; border-left: medium none;">-0.15</td> | + | style="border-top: medium none; border-left: medium none;"><small>-0.15</small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-top: medium none; border-left: medium none;">0.31</td> | + | style="border-top: medium none; border-left: medium none;"><small>0.31</small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-top: medium none; border-left: medium none;">-0.147</td> | + | style="border-top: medium none; border-left: medium none;"><small>-0.147</small></td> |
| <td class="xl6314788" | | <td class="xl6314788" |
- | style="border-top: medium none; border-left: medium none;">10</td> | + | style="border-top: medium none; border-left: medium none;"><small>10</small></td> |
- | </tr>
| + | |
- | <tr style="display: none;" height="0">
| + | |
- | <td style="width: 77pt;" width="103"></td>
| + | |
- | <td style="width: 48pt;" width="64"></td>
| + | |
- | <td style="width: 65pt;" width="86"></td>
| + | |
- | <td style="width: 48pt;" width="64"></td>
| + | |
- | <td style="width: 48pt;" width="64"></td>
| + | |
- | <td style="width: 48pt;" width="64"></td>
| + | |
- | <td style="width: 65pt;" width="86"></td>
| + | |
- | <td style="width: 48pt;" width="64"></td>
| + | |
| </tr> | | </tr> |
| </tbody> | | </tbody> |
| </table> | | </table> |
| <br /> | | <br /> |
- | </div>
| |
| <div class="cleared"></div> | | <div class="cleared"></div> |
| </div> | | </div> |
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