"
Team:Imperial College London/Wetlab/Protocols
From 2009.igem.org
(Difference between revisions)
(→Calibrations) |
(→Calibrations) |
||
| Line 22: | Line 22: | ||
* GFP fluorescence calibration | * GFP fluorescence calibration | ||
** [[Imperial_College_London/Wetlab/Protocols/FluorEx| <b>CA2</b>]]: External GFP fluorescence | ** [[Imperial_College_London/Wetlab/Protocols/FluorEx| <b>CA2</b>]]: External GFP fluorescence | ||
| - | ** [[ | + | ** [[Imperial_College_London/Wetlab/Protocols/FluorIn| <b>CA3</b>]]: Intracellular GFP fluorescence |
==[[Team:Imperial College London/Wetlab/Protocols/PromoterCharacterisation| Promoter Characterisation]]== | ==[[Team:Imperial College London/Wetlab/Protocols/PromoterCharacterisation| Promoter Characterisation]]== | ||
Latest revision as of 21:05, 20 October 2009
- Back to Hub
- Cloning Strategy
- Protocols
- BioBricks
- Results
- Notebook
Contents |
Protocols
Cell culturing/cloning protocols
- CC1: Miniprep
- CC2: Midiprep
- CC3: Making cells competent
- CC4: Ligation
- CC5: Transformation into BL21 using electrical shock
- CC6: Transformation into Top10 using chemical competence
- CC7: Making LB plates
- CC8: Extracting DNA from the registry
- CC9: Preparation of XL1-Blue electrocompetent cells & XL1-Blue quality control
- CC10: PCR
- CC11: SLIC
- CC12: Biobricking parts from oligos
- CC13: Genome Prep for Restriction Assay
Calibrations
- CA1: Optical Density Calibration
- GFP fluorescence calibration
Promoter Characterisation
- PP1: Lac Promoter Characterisation
Autoinduction
Protein Production
Encapsulation
- EN1: Colanic acid production amount
- EN2: Colanic acid protection from pH
- EN3: Trehalose production
Killing
- KI1: Thermoinduction
- KI2: Restriction Enzyme activity
