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Team:Wash U/Calendar-Home/29 June 2009
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(New page: Recipe for 0.8% agarose gel: 1) Add 0.8 g agarose to 50 mL 1X TAE buffer (1 ml 50X stock solution + 49 mL water) 2) Microwave on high for ~30-40 seconds (until agarose dissolved). Stop ...)
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(New page: Recipe for 0.8% agarose gel: 1) Add 0.8 g agarose to 50 mL 1X TAE buffer (1 ml 50X stock solution + 49 mL water) 2) Microwave on high for ~30-40 seconds (until agarose dissolved). Stop ...)
Newer edit →
Revision as of 19:18, 29 June 2009
Recipe for 0.8% agarose gel:
1) Add 0.8 g agarose to 50 mL 1X TAE buffer (1 ml 50X stock solution + 49 mL water)
2) Microwave on high for ~30-40 seconds (until agarose dissolved). Stop at 10-second intervals to swirl flask.
3) Cool, then add 2.5 microliters EtBr (wear gloves!). Swirl, and pour into gel tray. Let sit until ready for electrophoresis.
