Team:Wash U/Protocol
From 2009.igem.org
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'''Tris EDTA (TE) buffer''' | '''Tris EDTA (TE) buffer''' | ||
+ | *750 mL d-H20 | ||
+ | *242 g Tris base | ||
+ | *57.1 mL glacial acetic acid | ||
+ | *100 mL 0.5M EDTA (93.05 g EDTA in 500 mL d-H20, pH ~8.0) | ||
+ | *fill to 1 L | ||
+ | *no need to adjust pH but should be around 8.5 | ||
{{WashUbottom}} | {{WashUbottom}} |
Revision as of 19:23, 29 June 2009