Team:UNIPV-Pavia/Freezer

From 2009.igem.org

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Revision as of 13:24, 30 June 2009

EthanolPVanimation.gif

Biobrick/
Ligation Name		 Description Creation Date N° usage Strain Vector Quality Control
J23101(J101)
constitutive promoter
May 25
0
TOP10
J61002(Amp)
sequence ok (MIT)
J23102(J102)
constitutive promoter
May 25
0
TOP10
J61002(Amp)
sequence ok (MIT)
J23103(J103)
constitutive promoter
May 25
0
TOP10
J61002(Amp)
sequence ok (MIT)
J23104(J104)
constitutive promoter
May 25
0
TOP10
J61002(Amp)
sequence ok (MIT)
J23105(J105)
constitutive promoter
May 25
0
TOP10
J61002(Amp)
sequence ok (MIT)
J23106(J106)
constitutive promoter
May 25
0
TOP10
J61002(Amp)
sequence ok (MIT)
J23110(J110)
constitutive promoter
May 25
0
TOP10
J61002(Amp)
sequence ok (MIT)
J23112(J112)
constitutive promoter
May 25
0
TOP10
J61002(Amp)
sequence ok (MIT)
J23113(J113)
constitutive promoter
May 25
0
TOP10
J61002(Amp)
sequence ok (MIT)
J23114(J114)
constitutive promoter
May 25
0
TOP10
J61002(Amp)
sequence ok (MIT)
J23116(J116)
constitutive promoter
May 25
0
TOP10
J61002(Amp)
sequence ok (MIT)
J23117(J117)
constitutive promoter
May 25
0
TOP10
J61002(Amp)
sequence ok (MIT)
J23118(J118)
constitutive promoter
May 25
0
TOP10
J61002(Amp)
sequence ok (MIT)
E0240(GFP3)
GFP protein generatorRBS-GFP-TT
May 25
5
TOP10
pSB1A2
length ok (PCR)sequence ok (MIT)
K131009(E2)
colE2 operon:ceaB-ceiB-Tweak-celB-T (with RBS)
May 28
1
TOP10
pSB1AK3
sequence BAD in prefix,suffix,celB (MIT,BMR)
K117000(celB)
colE7 lysis gene
May 28
1
TOP10
pSB1A2
sequence ok (MIT)
K124017(lysis21)
Phage Lysis Cassette
May 28
0
TOP10
pSB1AK3
sequence BAD (MIT)
B0015(TT)
Double terminator
May 28
2
TOP10
pSB1AK3
sequence ok (MIT)
I732017 (RBS-lacZ)
RBS-lacZ
May 28
1
TOP10
pSB1A2
long part (MIT)
B0030
RBS 0.6
Jun 4
0
TOP10
pSB1A2
sequence ok (MIT,BMR)
B0031
RBS 0.07
Jun 4
0
TOP10
pSB1A2
sequence ok (MIT)
B0032
RBS 0.3
Jun 4
0
TOP10
pSB1A2
sequence ok (MIT)
B0033
RBS 0.01
Jun 4
0
TOP10
pSB1A2
sequence ok (MIT)
B0034
RBS 1
Jun 4
0
TOP10
pSB1A2
sequence ok (MIT)
P0440(P04)
RBS-tetR-TT
Jun 4
1
TOP10
pSB1A2
length ok (PCR)sequence ok (MIT)
R0040(Ptet)
Ptet
Jun 4
1
TOP10
pSB1A2
sequence ok (MIT)
T9002
Ptet-RBS-luxR-TT-Plux-RBS-GFP-TT
Jun 4
1
TOP10
pSB1A3
sequence BAD (MIT) but seems to work
J23100(J100bis)
constitutive promoter
Jun 4
1
TOP10
J61002(Amp)
sequence ok (MIT,BMR)
J23101(J101bis)
constitutive promoter
Jun 4
1
TOP10
J61002(Amp)
sequence ok (MIT)
J23118(J118bis)
constitutive promoter
Jun 4
1
TOP10
J61002(Amp)
sequence ok (MIT)
A1
J23100(Jun 4)-E0240(Pcon-RBS-GFP-TT)
Jun 11
1
TOP10
pSB1A2
length ok (PCR)
A2
J23101(Jun 4)-E0240(Pcon-RBS-GFP-TT)
Jun 11
0
TOP10
pSB1A2
length ok (PCR)
A3
I732017-B0015(RBS-lacZ-TT)
Jun 12
1
TOP10
pSB1AK3
length ok (PCR)sequence ok -long part- (BMR)
A4
P0440-R0040(RBS-tetR-TT-Ptet)
Jun 11
1
TOP10
pSB1A2
length ok (PCR)sequence ok (BMR)
A5
A4-A3(RBS-tetR-TT-Ptet-RBS-lacZ-TT)
Jun 19
0
TOP10
pSB1AK3
sequence ok -long part- (BMR)
A6
J23100(Jun 4)-A4(Pcon-RBS-tetR-TT-Ptet)
Jun 19
1
TOP10
pSB1A2
length ok (PCR)sequence ok (BMR)
A7
J23118(Jun 4)-E0240(Pcon-RBS-GFP-TT)
Jun 25
0
TOP10
pSB1A2
length ok (PCR)
R0011(Plac)
Plac
Jun 25
0
TOP10
pSB1A2
sequence ok (MIT)
K112808(ENTERO)
holin-lysozyme-T-Pconweak-antiholin-T (with RBSs)
Jun 25
0
TOP10
pSB1A2
sequence ok -long part- (MIT)

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