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Team:HKU-HKBU/Motor Preliminary Trials
From 2009.igem.org
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[[Image:HKU-HKBU_motor_results_1.png| left | thumb |200px|Step1. The fragmentation of the Immobolin-P membrane with the help of a mini-homogenizer]] | [[Image:HKU-HKBU_motor_results_1.png| left | thumb |200px|Step1. The fragmentation of the Immobolin-P membrane with the help of a mini-homogenizer]] | ||
| - | <gallery align="center"> | + | [[Image:Image:HKU-HKBU_motor_results_2.png| center | thumb |200px |Step2.The fragmentation of the Immobolin-P membrane with a generated shearing force by moistening and followed by vortexing]] |
| + | <gallery align="center"> | ||
Image:HKU-HKBU_motor_results_3.png | Image:HKU-HKBU_motor_results_3.png | ||
Image:HKU-HKBU_motor_results_4.png | Image:HKU-HKBU_motor_results_4.png | ||
Revision as of 07:39, 21 October 2009
Binding performance test using a membrane
We used Immobilon-P transfer membrane to evaluate the performance of streptavidin-biotin binding of bacteria onto a surface. Before cutting apart the membrane into small pieces, pre-activation should be applied to it first (see protocols membrane biotinylation). After the pre-activation, the membrane was first sheared into strings manually by using scissors, with the width and thickness being approximately 100μm. We then used Leica-crytomicrotome to cut the “threads” into even smaller fragments, with the length of which being 60μm. A binding performance testing device demension was aproximately 100μm×60μm×100μm. Since the surface of membrane had already been activated, the polar-expression bacteria could bind onto such biotin-coated motors.
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