Team:Kyoto/CiC/Notebook/0921-0925

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==0914-0918 : what to do. what to do. what to do. what to do. ==
==0914-0918 : what to do. what to do. what to do. what to do. ==
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===Monday, 14 August===
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===Monday, 21 September===
====To Do====
====To Do====
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*p-2-(1)(4)(7)(8)(10)
+
*HIV-TAT-(LALAAAA)3-generator
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**Miniprep
+
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**Restriction Enzyme Digestion
+
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*p-2-(1)(4)  E.X
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**PCR purification kit
+
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*p-2-(1)(7)(8)(10)  E.S
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**Gel extraction
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*4-(1)(2)(3)
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**Colony PCR
**Colony PCR
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**Insert check
 
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====Results====
====Results====
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=====p-2-(1)(4)(7)(8)(10)=====
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*消えた
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=====p-2-(1)(7)(8)(10)  E.S=====
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{{:Team:Kyoto/Pad_between_dates}}
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*Gel extraction kit
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[[Image:Kyoto_0914_PCR.png|250px]]
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{| class="table"
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|+Miniprep product conc.
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!NO.!!Sample name!!conc./(ng/ul)
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-
|-
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|1||p-2-(1)||9.3
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-
|-
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|2||p-2-(7)||25.9
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|-
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|3||p-2-(8)||8.6
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|-
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|4||p-2-(10)||7.5
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|}
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=====4-(1)(2)(3)=====
 
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[[Image:Kyoto_0914_PCR_2.png|250px]]
 
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*This result indicates that (1)a, (2)b and (3)a were inserted properly.
 
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{{:Team:Kyoto/Pad_between_dates}}
 
===Tuesday, 15 September===
===Tuesday, 15 September===
====To Do====
====To Do====

Revision as of 11:36, 21 October 2009

  1. Home
  2. CiC
  3. Notebook

0907-0911
constructing HIV-TAT-(LALAAAA)3-generator
0914-0918
constructing HIV-TAT-(LALAAAA)3-generator
0921-0925
constructing sig-GFP generator,sig-EGFP generator(HeLa)
0928-1002
constructing sig-GFP generator,sig-EGFP generator(HeLa)
1005-1009
constructing sig-GFP generator,sig-EGFP generator(HeLa)
1012-1016
Observation
1019-1023
Observation

0914-0918 : what to do. what to do. what to do. what to do.

Monday, 21 September

To Do

  • HIV-TAT-(LALAAAA)3-generator
    • Colony PCR

Results

  • 消えた

Tuesday, 15 September

To Do

Further to 14 Sept.

  • p-2-(1)(4)(7)(8)(10)
    • Miniprep
    • Restriction Enzyme Digestion
  • p-2-(1)(4) E.X
    • PCR purification kit
  • p-2-(1)(7)(8)(10) E.S
    • Gel extraction
  • 4-(1)(2)(3)
    • Colony PCR
    • Insert check

Wednesday, 16 September

To Do

    • ligation
    • transformation

Results

ligation
conc.
NO.Sample nameconc./(ng/ul)
111(stu1)103.2
Transformation

No colony was observed (090917 10:00)　

Thursday, 17 September

To Do

  • Make repetitive sequence (MPR)
  • Transformation(retry)

Retrieved from "http://2009.igem.org/Team:Kyoto/CiC/Notebook/0921-0925"