Team:PKU Beijing

From 2009.igem.org

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We are engineering our E.coli cells to process the correlation information of two environmental signal, similar to the process of classical conditioning in higher organisms. In our circuits we use a bistable switch as the memory module to represent the memory state; we also constructed a series of AND gates which can sense conditioned and unconditioned signals, and output a repressor protein to change the memory state of the bistable switch. In this way, our E.coli cells can convert the information on the concurrence of two signals to its memory. After the memory module is switched, given the conditioned stimulus, E.coli will activate its reporter module and thus exhibit the "conditioned response".
We are engineering our E.coli cells to process the correlation information of two environmental signal, similar to the process of classical conditioning in higher organisms. In our circuits we use a bistable switch as the memory module to represent the memory state; we also constructed a series of AND gates which can sense conditioned and unconditioned signals, and output a repressor protein to change the memory state of the bistable switch. In this way, our E.coli cells can convert the information on the concurrence of two signals to its memory. After the memory module is switched, given the conditioned stimulus, E.coli will activate its reporter module and thus exhibit the "conditioned response".
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[[Image:PKU_Logic_Circuit.png|500px|center|thumb|Logic Circuit of the System]]
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[[Image:PKU_Logic_Circuit.png|700px|center]]
[[Team:PKU_Beijing/Project|Start your journey into the fancy world of Conditioned Reflex Mimicking in E.coli]]
[[Team:PKU_Beijing/Project|Start your journey into the fancy world of Conditioned Reflex Mimicking in E.coli]]
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==='''Acknowledgement'''===  
==='''Acknowledgement'''===  

Revision as of 11:43, 21 October 2009

News

  • We will bring our seal to jamboree, also Chinese knots as souvenir.
PKU Seal.png
  • Our T-shirt has come out:
  • First Stage Assembly accomplished, moving on!
  • Assembly between the first two Modules has been recently accomplished by Shuke Wu! More detailed work is being carried on by him!
  • Our new site is up! Feel free to send any comment to c.z.tian AT gmail.com

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Conditioned Reflex Mimicking in E.coli

We are engineering our E.coli cells to process the correlation information of two environmental signal, similar to the process of classical conditioning in higher organisms. In our circuits we use a bistable switch as the memory module to represent the memory state; we also constructed a series of AND gates which can sense conditioned and unconditioned signals, and output a repressor protein to change the memory state of the bistable switch. In this way, our E.coli cells can convert the information on the concurrence of two signals to its memory. After the memory module is switched, given the conditioned stimulus, E.coli will activate its reporter module and thus exhibit the "conditioned response".

PKU Logic Circuit.png


Start your journey into the fancy world of Conditioned Reflex Mimicking in E.coli

Acknowledgement

The PKU iGEM2009 Team strongly acknowledges following institutes/companies


We also want to thank the following Professors of the Life Science School, for supporting us with equipments and reagents.

[http://www.bio.pku.edu.cn/faculty/chenjg/ Prof. Jianguo Chen] [http://www.bio.pku.edu.cn/faculty/liud/ Prof. Dong Liu] [http://www.bio.pku.edu.cn/faculty/wangsq/ Prof. Shiqiang Wang] [http://www.bio.pku.edu.cn/faculty/wangyp/ Prof. Yiping Wang] [http://www.bio.pku.edu.cn/faculty/yulc/ Prof. Longchuan Yu] [http://www.bio.pku.edu.cn/faculty/zhangy/ Prof. Yan Zhang]



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