Team:HKU-HKBU/Motor Preliminary Trials

From 2009.igem.org

(Difference between revisions)

Revision as of 12:01, 21 October 2009

Binding performance test using a membrane

Before the final version of motor that was using the photolithography photoetching method based on silicon, we did several trials to approach the qualified micromotor with the characteristics as: 1.It should be shaped into very refined tiny structures. 2.It should be able to undergo chemical modifications to coat the biotin on the surface. 3.It should be rigid and stable in the bacterial medium. Here we report our trials by using Immobolin-P membrane which is easy to cut and coat with biotin. 1.The fragmentation of the Immobolin-P membrane with the help of a mini-homogenizer The Immobolin-P membrane was first made wet and consequently homogenized with a mini-homogenizer. Results: The membrane could not be broken into small pieces.

Fig 1. The fragmentation of the Immobolin-P membrane with the help of a mini-homogenizer

Step2.The fragmentation of the Immobolin-P membrane with a generated shearing force by moistening and followed by vortexing

@@ Line 5: / Line 5: @@
 ==Binding performance test using a membrane==
-We used Immobilon-P transfer membrane to evaluate the performance of streptavidin-biotin binding of bacteria onto a surface. Before cutting apart the membrane into small pieces, pre-activation was applied to it first  (see protocol [[Team:HKU-HKBU/Protocols#Membrane_Biotinylation | membrane biotinylation]]). After the pre-activation, the membrane was first sheared into strings manually by scissors, with the width and thickness being approximately 100μm. We then used Leica-crytomicrotome to cut the “threads” into even smaller fragments, with the length of which being 60μm. The dimension of the binding performance testing device is aproximately 100μm×60μm×100μm. Since the surface of membrane had already been activated, the polar-expression bacteria could bind onto such biotin-coated motors.
+Before the final version of motor that was using the [[Team:HKU-HKBU/Protocols#Photolithography | photolithography photoetching method]] based on silicon, we did several trials to approach the qualified micromotor with the characteristics as:
+.It should be shaped into very refined tiny structures.
-[[Image:HKU-HKBU_motor_results_1.png| left | thumb |200px|Step1. The fragmentation of the Immobolin-P membrane with the help of a mini-homogenizer]]
+.It should be able to undergo chemical modifications to coat the biotin on the surface.
+.It should be rigid and stable in the bacterial medium.
+Here we report our trials by using Immobolin-P membrane which is easy to cut and coat with biotin.
+.The fragmentation of the Immobolin-P membrane with the help of a mini-homogenizer
+The Immobolin-P membrane was first made wet and consequently homogenized with a mini-homogenizer.
+Results: The membrane could not be broken into small pieces.
+[[Image:HKU-HKBU_motor_results_1.png| right | thumb |200px|Fig 1. The fragmentation of the Immobolin-P membrane with the help of a mini-homogenizer]]
 [[Image:HKU-HKBU_motor_results_2.png‎| center | thumb |200px |Step2.The fragmentation of the Immobolin-P membrane with a generated shearing force by moistening and followed by vortexing]]
 <gallery align="center">
@@ Line 14: / Line 20: @@
 Image:HKU-HKBU_motor_results_5.png‎
 Image:HKU-HKBU_motor_results_6.png‎</gallery>
 {{Team:HKU-HKBU/footer}}