Team:Kyoto/CiC/Notebook/0921-0925

From 2009.igem.org

(Difference between revisions)
(Monday, 21 September)
(Monday, 21 September)
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===Monday, 21 September===
===Monday, 21 September===
====To Do====
====To Do====
-
*No experiments were undertaken
+
*HIV-TAT-(LALAAAA)3-generator
 +
**miniprep
 +
*TIM23complex signal::EGFP generator
 +
**PEGFP-N3 VECTOR (EGFP generator)
 +
====Results====
 +
*消えた
{{:Team:Kyoto/Pad_between_dates}}
{{:Team:Kyoto/Pad_between_dates}}

Revision as of 12:23, 21 October 2009

  1. Home
  2. CiC
  3. Notebook

0907-0911
constructing HIV-TAT-(LALAAAA)3-generator
0914-0918
constructing HIV-TAT-(LALAAAA)3-generator
0921-0925
constructing sig-GFP generator,sig-EGFP generator(HeLa)
0928-1002
constructing sig-GFP generator,sig-EGFP generator(HeLa)
1005-1009
constructing sig-GFP generator,sig-EGFP generator(HeLa)
1012-1016
Observation
1019-1023
Observation

0914-0918 : what to do. what to do. what to do. what to do.


Monday, 21 September

To Do

  • HIV-TAT-(LALAAAA)3-generator
    • miniprep
  • TIM23complex signal::EGFP generator
    • PEGFP-N3 VECTOR (EGFP generator)

Results

  • 消えた



Tuesday, 22 September

To Do

  • HIV-TAT-(LALAAAA)3-generator
    • miniprep
  • TIM23complex signal::EGFP generator
    • PEGFP-N3 VECTOR (EGFP generator)

Results

  • 消えた



Wednesday, 23 September

To Do

    • ligation
    • transformation

Results

ligation
conc.
NO.Sample nameconc./(ng/ul)
111(stu1)103.2
Transformation

No colony was observed (090917 10:00) 



Thursday, 17 September

To Do

  • Make repetitive sequence (MPR)
  • Transformation(retry)