Team:UNICAMP-Brazil/Protocols

From 2009.igem.org

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'''[[Team:UNICAMP-Brazil/Protocols/Preparation of electrocompetent S. cereviseae|13. Preparation of electrocompetent S. cerevisiae]]'''
'''[[Team:UNICAMP-Brazil/Protocols/Preparation of electrocompetent S. cereviseae|13. Preparation of electrocompetent S. cerevisiae]]'''
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'''[[Team:UNICAMP-Brazil/Protocols/Digestion reaction|14. Digestion Reaction]]'''
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'''[[Team:UNICAMP-Brazil/Protocols/Restriction reaction|14. Restriction Reaction]]'''
'''[[Team:UNICAMP-Brazil/Protocols/pGEMStrategy|15. pGEM cloning strategy]]'''
'''[[Team:UNICAMP-Brazil/Protocols/pGEMStrategy|15. pGEM cloning strategy]]'''

Revision as of 12:25, 21 October 2009

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Protocols

1. Genomic DNA Extraction

2. Mini-Prep

3. Transformation

4. Preparation of electrocompetent E. coli

5. Yeast DNA Extraction

6. Yeast RNA Extraction

7. Purification of DNA fragments from agarose gels

8. Isolation of RNA using Trizol Reagent (Invitrogen)

9. SAP Dephosphorylation

10. CIAP Dephosphorylation

11. T4 DNA Ligase

12. Electroelution

13. Preparation of electrocompetent S. cerevisiae

14. Restriction Reaction

15. pGEM cloning strategy

16. Expression strategy in Yeast




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