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SNOWDRIFT/Proof of concept
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We tried to test this export mechanism using the following biobricks: | We tried to test this export mechanism using the following biobricks: | ||
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Both these constructs are designed to secrete the Green Fluorescent Protein in to the extracellular environment. | Both these constructs are designed to secrete the Green Fluorescent Protein in to the extracellular environment. | ||
Revision as of 14:16, 21 October 2009
Proof of Concept:
In order to establish cell-cell communication protein signalling can come in handy. We attempted to do this by attaching what we call a 'secretion tag' to our protein of interest. This 'tag' allows secretion of proteins.
This tag is designed by using N-terminal sequences from proteins which are exported using the TAT Pathway.
We tried to test this export mechanism using the following biobricks:
File:Snowdriftproofofconcept.jpeg
Both these constructs are designed to secrete the Green Fluorescent Protein in to the extracellular environment.
