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SNOWDRIFT/Proof of concept
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In order to establish cell-cell communication protein signalling can come in handy. | In order to establish cell-cell communication protein signalling can come in handy. | ||
We attempted to do this by attaching what we call a 'secretion tag' to our protein of interest. This 'tag' allows secretion of proteins. | We attempted to do this by attaching what we call a 'secretion tag' to our protein of interest. This 'tag' allows secretion of proteins. | ||
Revision as of 14:21, 21 October 2009
Proof of Concept
In order to establish cell-cell communication protein signalling can come in handy.
We attempted to do this by attaching what we call a 'secretion tag' to our protein of interest. This 'tag' allows secretion of proteins.
This tag is designed by using N-terminal sequences from proteins which are exported using the TAT Pathway.
We tried to test this export mechanism using the following biobricks:
Both these constructs are designed to secrete the Green Fluorescent Protein in to the extracellular environment.
