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Team:ArtScienceBangalore/Parts
From 2009.igem.org
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<strong>Here is the part that we submitted to the Standard Parts Registry: <br> <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K221000">BBa_K221000</a></strong><br> | <strong>Here is the part that we submitted to the Standard Parts Registry: <br> <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K221000">BBa_K221000</a></strong><br> | ||
| - | < | + | <h5><i>This helps our construct synthesize geosmin, the enzyme that smells like rain.</i></h5><br> |
| - | This part codes N-terminal (366 amino acid) domain of the SCO6073 gene from S. coelicolor A3.<br> Expression of this domain gives a fully functional germacradienol synthase with steady-state <br> catalytic parameters similar to those of the full-length protein that converts farnesyl diphosphate(FPP) to Geosmin*. | + | This part codes N-terminal (366 amino acid) domain of the SCO6073 gene from S. coelicolor A3.<br> Expression of this domain gives a fully functional germacradienol synthase with steady-state <br> catalytic parameters similar to those of the full-length protein that converts farnesyl diphosphate(FPP) to Geosmin*.<br><br> |
| - | <strong> Amino acid sequence:</strong> | + | <strong> <b>Amino acid sequence:</b> </strong> |
| - | + | MTQQPFQLPHFYLPHPARLNPHLDEARAHSTTWAREMGMLEGSGVWEQSDLEAHDYGLLCAYTHPDCDGPALSLITDWYVWVFFFDDHFLE<br> | |
| - | + | KYKRSQDRLAGKAHLDRLPLFMPLDDAAGMPEPRNPVEAGLADLWTRTVPAMSADWRRRFAVATEHLLNESMWELSNINEGRVANPVEYIE<br> | |
| - | + | MRRKVGGAPWSAGLVEYATAEVPAAVAGTRPLRVLMETFSDAVHLRNDLFSYQREVEDEGELSNGVLVLETFFGCTTQEAADLVNDVLTSR<br> | |
| - | + | LHQFEHTAFTEVPAVALEKGLTPLEVAAVGAYTKGLQDWQSGGHEWHMRSSRYMNKGERPLAGWQALTGPGTSAADVGALLADAVAQRARSYT | |
| - | + | ||
| - | + | ||
| - | + | ||
</div> | </div> | ||
Revision as of 15:05, 21 October 2009
Parts
Here is the part that we submitted to the Standard Parts Registry:
BBa_K221000
This part codes N-terminal (366 amino acid) domain of the SCO6073 gene from S. coelicolor A3.
Expression of this domain gives a fully functional germacradienol synthase with steady-state
catalytic parameters similar to those of the full-length protein that converts farnesyl diphosphate(FPP) to Geosmin*.
Amino acid sequence: MTQQPFQLPHFYLPHPARLNPHLDEARAHSTTWAREMGMLEGSGVWEQSDLEAHDYGLLCAYTHPDCDGPALSLITDWYVWVFFFDDHFLE
KYKRSQDRLAGKAHLDRLPLFMPLDDAAGMPEPRNPVEAGLADLWTRTVPAMSADWRRRFAVATEHLLNESMWELSNINEGRVANPVEYIE
MRRKVGGAPWSAGLVEYATAEVPAAVAGTRPLRVLMETFSDAVHLRNDLFSYQREVEDEGELSNGVLVLETFFGCTTQEAADLVNDVLTSR
LHQFEHTAFTEVPAVALEKGLTPLEVAAVGAYTKGLQDWQSGGHEWHMRSSRYMNKGERPLAGWQALTGPGTSAADVGALLADAVAQRARSYT
BBa_K221000
This helps our construct synthesize geosmin, the enzyme that smells like rain.
This part codes N-terminal (366 amino acid) domain of the SCO6073 gene from S. coelicolor A3.
Expression of this domain gives a fully functional germacradienol synthase with steady-state
catalytic parameters similar to those of the full-length protein that converts farnesyl diphosphate(FPP) to Geosmin*.
Amino acid sequence: MTQQPFQLPHFYLPHPARLNPHLDEARAHSTTWAREMGMLEGSGVWEQSDLEAHDYGLLCAYTHPDCDGPALSLITDWYVWVFFFDDHFLE
KYKRSQDRLAGKAHLDRLPLFMPLDDAAGMPEPRNPVEAGLADLWTRTVPAMSADWRRRFAVATEHLLNESMWELSNINEGRVANPVEYIE
MRRKVGGAPWSAGLVEYATAEVPAAVAGTRPLRVLMETFSDAVHLRNDLFSYQREVEDEGELSNGVLVLETFFGCTTQEAADLVNDVLTSR
LHQFEHTAFTEVPAVALEKGLTPLEVAAVGAYTKGLQDWQSGGHEWHMRSSRYMNKGERPLAGWQALTGPGTSAADVGALLADAVAQRARSYT
