Team:LCG-UNAM-Mexico/Resources/Strains

E. coli Strains

Strains handed by Giuseppe Bertani

• C-1a.- Main strain for working with phage P2 and P4. This is also the mother strain for all the modified phage-containing derivatives. Its genetic map is really close to E. coli K12 "pop" strain. Its genome is being sequenced.

• C-117.- Lysogenic for P2

• C-1906.- Lysogenic for P4.

Strains handed by Richard Calendar

C-331 /arg/-am/1/ /tonA-trp/D, str-r (P2) (P4 /imp/) The abbreviation "imp" means "improved plaques."

C-520 F^+ /supD60/, used for growth and plaque assay of P2.

C-1895 F^+ /supD60/ (P2 /lg/), which we use to grow and sometimes assay for P4. The symbol "lg" denotes large plaques.

C-2423 /trp/-am /arg/-am/1/ str-r (P2 /lg/ del/1/) This strain is used to get the best P4 plaques. The symbol del1 denotes a deletion of the /old /gene, which interferes with the growth of phage lambda and kills /recB-/ bacteria.

Strain variants obtained in our lab

C-1895 sp+.- this slow-growing strain can be used for obtaining an excelent stock of P4 sid1

Expression Strains

BL21(DE3)pLysS:

BL21 strains are the most widely used hosts for protein expression from pET recombinants and have the advantage of being deficient in both lon and ompT proteases.

DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.

pLysS strains express T7 lysozyme, which further suppresses basal expression of T7 RNA polymerase prior to induction, thus stabilizing pET recombinants encoding target proteins that affect cell growth and viability. Genotype: F- ompT hsdSB(rB- mB-) gal dcm (DE3) pLysS (CamR)

Yeast strains

TERRIBLY UNCOUNTABLE ANTIBIOTIC RESISTANTS!!!