"
Team:LCG-UNAM-Mexico/Resources/Strains
From 2009.igem.org
(Difference between revisions)
(→Strains handed by Giuseppe Bertani) |
|||
| Line 17: | Line 17: | ||
===Strains handed by Richard Calendar=== | ===Strains handed by Richard Calendar=== | ||
| - | C-331 /arg/-am/1/ /tonA-trp/D, str-r (P2) (P4 /imp/) | + | *C-331 /arg/-am/1/ /tonA-trp/D, str-r (P2) (P4 /imp/). The abbreviation "imp" means "improved plaques." |
| - | The abbreviation "imp" means "improved plaques." | + | |
| - | C-520 F^+ /supD60/, used for growth and plaque assay of P2. | + | *C-520 F^+ /supD60/, used for growth and plaque assay of P2. |
| - | C-1895 F^+ /supD60/ (P2 /lg/), which we use to grow and sometimes assay | + | *C-1895 F^+ /supD60/ (P2 /lg/), which we use to grow and sometimes assay for P4. The symbol "lg" denotes large plaques. |
| - | for P4. The symbol "lg" denotes large plaques. | + | |
| - | C-2423 /trp/-am /arg/-am/1/ str-r (P2 /lg/ del/1/) | + | *C-2423 /trp/-am /arg/-am/1/ str-r (P2 /lg/ del/1/). This strain is used to get the best P4 plaques. The symbol del1 denotes a |
| - | This strain is used to get the best P4 plaques. The symbol del1 denotes a | + | deletion of the /old /gene, which interferes with the growth of phage lambda and kills /recB-/ bacteria. |
| - | deletion of the /old /gene, which interferes with the growth of phage | + | |
| - | lambda and kills /recB-/ bacteria. | + | |
===Strain variants obtained in our lab=== | ===Strain variants obtained in our lab=== | ||
| - | C-1895 sp+.- this slow-growing strain can be used for obtaining an excelent [[Team:LCG-UNAM-Mexico:Journals:Nando's#September 19, 2009|stock of P4 sid1]] | + | *C-1895 sp+.- this slow-growing strain can be used for obtaining an excelent [[Team:LCG-UNAM-Mexico:Journals:Nando's#September 19, 2009|stock of P4 sid1]] |
===Expression Strains=== | ===Expression Strains=== | ||
| Line 38: | Line 34: | ||
BL21(DE3)pLysS: | BL21(DE3)pLysS: | ||
| - | BL21 strains are the most widely used hosts for protein expression from pET recombinants and have the advantage of being deficient in both lon and ompT proteases. | + | *BL21 strains are the most widely used hosts for protein expression from pET recombinants and have the advantage of being deficient in both lon and ompT proteases. |
| - | DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG. | + | *DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG. |
| - | pLysS strains express T7 lysozyme, which further suppresses basal expression of T7 RNA polymerase prior to induction, thus stabilizing pET recombinants encoding target proteins that affect cell growth and viability. Genotype: F- ompT hsdSB(rB- mB-) gal dcm (DE3) pLysS (CamR) | + | *pLysS strains express T7 lysozyme, which further suppresses basal expression of T7 RNA polymerase prior to induction, thus stabilizing pET recombinants encoding target proteins that affect cell growth and viability. Genotype: F- ompT hsdSB(rB- mB-) gal dcm (DE3) pLysS (CamR) |
===Yeast strains=== | ===Yeast strains=== | ||
Revision as of 15:49, 21 October 2009