Revision as of 16:20, 21 October 2009

HOME	TEAM	PROJECT	SOFTWARE	MODELING	WET LAB	PARTS	HUMAN PRACTICE	JUDGING CRITERIA

Project Summary

Which is our idea?

The project aims to design a new device to control the synthesis of a protein in Escherichia coli regardless of the protein to be controlled. This "general-purpose" standard device acts on translation to allow a faster silencing of protein expression with respect to standard regulated promoters. Our device was named T-Rex (Trans Repressor of Expression). It consists of two new BioBricks, i.e. the Trans-repressor and the Cis-repressing.

How can we achieve our goal?

T-Rex device consists of two new BioBricks:

CIS-repressing, to be assembled upstream of the target protein coding sequence; it contains a ribosomal binding site (RBS);

TRANS-repressor, to be placed under the control of a different promoter; it is complementary to the CIS-repressing, and contains a RBS cover in two versions of different length (4 and 7 nucleotides).

CIS-repressing and TRANS-repressor were designed using our [[Team:Bologna/Software#1|BASER]] tool.

Transcription of the target gene produces a mRNA strand, starting with the Cis element, which is translated into proteins by ribosome. Trans’ promoter induction produces a transcript that binds with the Cis part. The RNA duplex prevents ribosome from binding to RBS, repressing protein synthesis. Thus, the TRANS-repressor amount regulates the gene mRNA translation rate.

How can we test the device?

In order to test and characterize our T-REX device, we developed the following genetic circuit:

The T-REX device is proposed as a universal and fast switch in synthetic gene circuits.

More details about our work in the Project section.

Acknowledgements

[http://www.unibo.it/Portale/default.htm University of Bologna]

[http://serinar.criad.unibo.it Ser.In.Ar. Cesena]

Cultural Association San Sebastiano

Up

@@ Line 15: / Line 15: @@
 <html>
 <font face="Calibri" font size="4" color="#000000">
-The project aims to design a new device to control the synthesis of a protein in Escherichia coli regardless of the protein to be controlled. This "general-purpose" standard device, by acting on translation, could allow a faster silencing of protein expression with respect to standard regulated promoters. Our device was named <b>T-Rex</b> (<b>T</b>rans <b>R</b>epressor of <b>Ex</b>pression). It consists of two new BioBricks, i.e. the Trans-repressor and the Cis-repressing.
+The project aims to design a new device to control the synthesis of a protein in Escherichia coli regardless of the protein to be controlled. This "general-purpose" standard device acts on translation to allow a faster silencing of protein expression with respect to standard regulated promoters. Our device was named <b>T-Rex</b> (<b>T</b>rans <b>R</b>epressor of <b>Ex</b>pression). It consists of two new BioBricks, i.e. the Trans-repressor and the Cis-repressing.
 </font></html>