Team:Newcastle/ Stochastic Switch cloning strategy

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We have the following cloning strategies for testing our construct.
We have the following cloning strategies for testing our construct.
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==Lab Work Strategies==
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====Cloning strategies: Stochastic switch====
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====Cloning strategies====
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The following is the clone manager file for our synthesised brick; using clone manager it is easy to simulate digests and ligation reactions, in order to make sure the enzymes you use in the will give the result you expect.
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Currently in the lab we are half way to acheiving a complete manual brick which is the degradation controller. In the lab so far, the stochastic switch team have:
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*Rehydrated, transformed, and miniprepped the biiobricks involved in promoter replacement, as well as frozen down these ''E.coli'' strains into the TPA collection.
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*Prepared the pSB1AT3 plasmid backbone (restriction digest, gel extraction) ready for ligation.
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*Have successful PCR products for the sac region (''bacillus'') and sspb (''E.coli''), and a possible success for the ara region.
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* Have done digests for sspb and sac, sspb has been extracted from the gel and is due for cloning.  
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[[Image:Team NewcastleClone manager Synthesised.png|center|600px]]
[[Image:Team NewcastleClone manager Synthesised.png|center|600px]]

Revision as of 16:28, 21 October 2009

Stochastic Switch Cloning Strategy

We have the following cloning strategies for testing our construct.

Cloning strategies: Stochastic switch

The following is the clone manager file for our synthesised brick; using clone manager it is easy to simulate digests and ligation reactions, in order to make sure the enzymes you use in the will give the result you expect.

Team NewcastleClone manager Synthesised.png



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