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Duke University/4 June 2009
From 2009.igem.org
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==June 4, 2009== | ==June 4, 2009== | ||
| + | *E: Gel to check Bioplastics #1-5 | ||
| + | *R: All correct sized bands | ||
| + | *C: Use one as template for PCR | ||
| - | + | *E: PCR with Bioplastics #5 as template | |
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| - | E: PCR with Bioplastics #5 as template | + | |
| + | <pre> | ||
5x HF buffer 5 ul | 5x HF buffer 5 ul | ||
| - | |||
dNTPs 2 ul | dNTPs 2 ul | ||
| - | |||
forward primer 1.25 ul | forward primer 1.25 ul | ||
| - | |||
reverse primer 1.25 ul | reverse primer 1.25 ul | ||
| - | |||
DNA template (plasmid) 0.5 ul | DNA template (plasmid) 0.5 ul | ||
| - | |||
Phusion DNA polymerase 0.3 ul | Phusion DNA polymerase 0.3 ul | ||
| - | |||
H2O 14.7 ul | H2O 14.7 ul | ||
25 ul | 25 ul | ||
| - | |||
98°C 30s 1x | 98°C 30s 1x | ||
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4 phaC 1.9 kb | 4 phaC 1.9 kb | ||
| - | </ | + | </pre> |
Revision as of 16:28, 21 October 2009
June 4, 2009
- E: Gel to check Bioplastics #1-5
- R: All correct sized bands
- C: Use one as template for PCR
- E: PCR with Bioplastics #5 as template
5x HF buffer 5 ul dNTPs 2 ul forward primer 1.25 ul reverse primer 1.25 ul DNA template (plasmid) 0.5 ul Phusion DNA polymerase 0.3 ul H2O 14.7 ul 25 ul 98°C 30s 1x 98°C 15s 55°C 30s 72°C 1m 10s 35x 72°C 5m 4°C infinity 1x Fragment # Name Size/Length 1 pASKphaA 4.2 kb 2 phaB 900 bp 3 Termcat2 1.7 kb 4 phaC 1.9 kb
