Team:HKU-HKBU/29 June 2009

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(New page: 1, test E.coli BL21 swimming ability (QQ) 2, search E.coli strains with complete LPS: MG1655 (Samuel) 3. transfer plasmid pET-sp-strp-AIDA (Alex and Brooks) -tranfer to BL21 ...)
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Revision as of 09:02, 3 July 2009

1, test E.coli BL21 swimming ability (QQ)

2, search E.coli strains with complete LPS: MG1655 (Samuel)

3. transfer plasmid pET-sp-strp-AIDA (Alex and Brooks) 

 -tranfer to BL21
 -run gel
 -Western blotting, anti-gfp
 -Fluorescence microscope

4. pET-sp-strp-AIDA: remove gfp (James and Amy) Biotin-labeled antibody: immunostaining

5. Speed control: control cheZ (QQ and Nova) 

 cheZ under promoter of arabinose or IPTG
 -transfer plasmid into MGS (cheZ-)
 -Western blot different IPTG conc. to see cheZ expression level
 -swimming speed assay under different IPTG conc.

6. Particle: biotin-labeled (Bosco)

7. Biobricks 

 AIDA   strep  cheZ   cheYp

Promoter---rbs---cheZ---double terminator PET---sp---gfp---strep [PT7----rbs]----[sp----gfp---strep]---[terminator] 

          (fusion protein)


Backbone: PSB1A2, using PMB1 as origin

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