"
Duke University/18 June 2009
From 2009.igem.org
(Difference between revisions)
| Line 1: | Line 1: | ||
| - | + | [[Team:Duke | Return]] | |
| - | + | ||
| + | ==June 18, 2009== | ||
| + | *E: Assembly PCR, Gels to check PCRs | ||
| + | |||
| + | <pre> | ||
| + | 2x Phusion mix 12.5 ul | ||
| + | insert 7.14 ul | ||
| + | H2O 5.36 ul | ||
| + | 25 ul | ||
| + | |||
| + | 98°C 30s 1x | ||
| + | 98°C 10s | ||
| + | 70-55°C 5s | ||
| + | 72°C 30s 25x | ||
| + | 72°C 2m | ||
| + | 4°C infinity 1x | ||
| + | |||
| + | Fragment # Name Size/Length (bp) Concentration (ng/ul) Mass (ng) Volume (ul) | ||
| + | 1 pASKphaA 4215 41 194 4.73 | ||
| + | 2 phaB 896 68 41 0.60 | ||
| + | 3 Termcat2 1701 69 78 1.13 | ||
| + | 4 phaC 1872 126 86 0.68 | ||
| + | ~400 7.14 | ||
| + | </pre> | ||
| + | |||
| + | |||
| + | Length of plasmid: 8360 bp + 4 Histags * 6 aa * 3 bp = 8432 bp | ||
| + | *R: Smear because of misformed gel, no band | ||
| + | *C: Change conditions | ||
Revision as of 16:42, 21 October 2009
June 18, 2009
- E: Assembly PCR, Gels to check PCRs
2x Phusion mix 12.5 ul insert 7.14 ul H2O 5.36 ul 25 ul 98°C 30s 1x 98°C 10s 70-55°C 5s 72°C 30s 25x 72°C 2m 4°C infinity 1x Fragment # Name Size/Length (bp) Concentration (ng/ul) Mass (ng) Volume (ul) 1 pASKphaA 4215 41 194 4.73 2 phaB 896 68 41 0.60 3 Termcat2 1701 69 78 1.13 4 phaC 1872 126 86 0.68 ~400 7.14
Length of plasmid: 8360 bp + 4 Histags * 6 aa * 3 bp = 8432 bp
- R: Smear because of misformed gel, no band
- C: Change conditions
