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| - | <head>
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| - | <meta content="text/html;charset=ISO-8859-1" http-equiv="Content-Type">
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| - | <title>Model 1</title>
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| - | </head>
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| - | <body>
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| - | <div style="text-align: center;"><span
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| - | style="color: rgb(255, 255, 153);"><span
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| - | style="font-family: "Times New Roman","serif";"><span
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| - | style="font-weight: bold;">=</span></span></span><b
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| - | style="color: rgb(255, 255, 153);"><span
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| - | style="font-size: 13.5pt; font-family: "Times New Roman","serif";"><big><big><big><big><a
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| - | href="https://2009.igem.org/Team:UC_Davis"><img alt=""
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| - | src="https://static.igem.org/mediawiki/2009/a/a4/UCDAVIS_PIC3.png"
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| - | style="border: 0px solid ; width: 83px; height: 36px;"></a> </big></big></big></big></span></b><a
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| - | href="https://2009.igem.org/Team:UC_Davis/About_Us"><b
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| - | style="color: rgb(255, 255, 153);"><span
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| - | style="font-size: 13.5pt; font-family: "Times New Roman","serif";"><big><big><big><big><img
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| - | alt="" src="https://static.igem.org/mediawiki/2009/d/d1/UCDAVIS_PIC4.png"
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| - | style="border: 0px solid ; width: 82px; height: 36px;"></big></big></big></big></span></b></a><b
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| - | style="color: rgb(255, 255, 153);"><span
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| - | style="font-size: 13.5pt; font-family: "Times New Roman","serif";"></span></b><b
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| - | style="color: rgb(255, 255, 153);"><span
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| - | style="font-size: 13.5pt; font-family: "Times New Roman","serif";"></span></b><b
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| - | style="color: rgb(255, 255, 153);"><span
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| - | style="font-size: 13.5pt; font-family: "Times New Roman","serif";"><big><big><big><big>
| + | |
| - | </big></big></big></big></span></b><b style="color: rgb(255, 255, 153);"><span
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| - | style="font-size: 13.5pt; font-family: "Times New Roman","serif";"><big><big><big><big><a
| + | |
| - | href="https://2009.igem.org/Team:UC_Davis/Project"><img alt=""
| + | |
| - | src="https://static.igem.org/mediawiki/2009/b/b9/UCDAVIS_PIC8.png"
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| - | style="border: 0px solid ; width: 78px; height: 36px;"></a> </big></big></big></big></span></b><b
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| - | style="color: rgb(255, 255, 153);"><span
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| - | style="font-size: 13.5pt; font-family: "Times New Roman","serif";"><big><big><big><big><a
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| - | href="https://2009.igem.org/Team:UC_Davis/Notebook"><img alt=""
| + | |
| - | src="https://static.igem.org/mediawiki/2009/2/2f/UCDAVIS_PIC5.png"
| + | |
| - | style="border: 0px solid ; width: 81px; height: 36px;"></a> </big></big></big></big></span></b><b
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| - | style="color: rgb(255, 255, 153);"><span
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| - | style="font-size: 13.5pt; font-family: "Times New Roman","serif";"></span></b><b
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| - | style="color: rgb(255, 255, 153);"><span
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| - | style="font-size: 13.5pt; font-family: "Times New Roman","serif";"><big><big><big><big><a
| + | |
| - | href="https://2009.igem.org/Team:UC_Davis/Parts"><img alt=""
| + | |
| - | src="https://static.igem.org/mediawiki/2009/a/a6/UCDAVIS_PIC6.png"
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| - | style="border: 0px solid ; width: 78px; height: 37px;"></a></big></big></big></big></span></b><b
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| - | style="color: rgb(0, 0, 0);"><span
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| - | style="font-size: 13.5pt; font-family: "Times New Roman","serif";"></span></b></div>
| + | |
| - | <hr style="width: 100%; height: 2px;"><big><big><big><span
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| - | style="text-decoration: underline; font-weight: bold;"><small>Secretion
| + | |
| - | Model 5:<br>
| + | |
| - | <br>
| + | |
| - | </small></span></big></big></big><big><span style="font-style: italic;">Click
| + | |
| - | on an
| + | |
| - | individual part for more information.<br>
| + | |
| - | </span></big>
| + | |
| - | <div style="text-align: center;"><big><big><big><span
| + | |
| - | style="font-weight: bold;"><small><span
| + | |
| - | style="text-decoration: underline; background-color: rgb(255, 255, 0);"><a
| + | |
| - | href="#LacI"><img alt=""
| + | |
| - | src="https://static.igem.org/mediawiki/2009/4/4e/UCDAVIS_PROMOTER_1.png"
| + | |
| - | style="border: 0px solid ; width: 242px; height: 58px; background-color: rgb(255, 255, 255);"></a><a
| + | |
| - | href="#RBS"><img alt=""
| + | |
| - | src="https://static.igem.org/mediawiki/2009/1/15/UCDAVIS_RBS1.png"
| + | |
| - | style="border: 0px solid ; width: 89px; height: 81px; background-color: rgb(255, 255, 255);"></a><a
| + | |
| - | href="#SS"><img alt=""
| + | |
| - | src="https://static.igem.org/mediawiki/2009/9/9d/UCDAVIS_SS1.png"
| + | |
| - | style="border: 0px solid ; width: 94px; height: 69px; background-color: rgb(255, 255, 255);"></a><img
| + | |
| - | style="width: 106px; height: 59px; background-color: rgb(255, 255, 255);"
| + | |
| - | alt="" src="https://static.igem.org/mediawiki/2009/0/0c/UCDAVIS_GENE1.png"><a
| + | |
| - | href="#His"><img alt=""
| + | |
| - | src="https://static.igem.org/mediawiki/2009/c/c0/UCDAVIS_6HIS.png"
| + | |
| - | style="border: 0px solid ; width: 134px; height: 66px; background-color: rgb(255, 255, 255);"></a><a
| + | |
| - | href="#Terminator"><img alt=""
| + | |
| - | src="https://static.igem.org/mediawiki/2009/c/ca/UCDAVIS_STOP.png"
| + | |
| - | style="border: 0px solid ; width: 94px; height: 83px; background-color: rgb(255, 255, 255);"></a><br>
| + | |
| - | </span></small></span></big></big></big><big><big><big><span
| + | |
| - | style="font-weight: bold;"><small><span
| + | |
| - | style="text-decoration: underline; background-color: rgb(255, 255, 0);"><br>
| + | |
| - | </span>a.<a href="#LacI"><span style="text-decoration: underline;"><img
| + | |
| - | alt=""
| + | |
| - | src="https://static.igem.org/mediawiki/2009/4/4e/UCDAVIS_PROMOTER_1.png"
| + | |
| - | style="border: 0px solid ; width: 200px; height: 52px;"></span></a><span
| + | |
| - | style="text-decoration: underline; background-color: rgb(255, 255, 0);"><a
| + | |
| - | href="#RBS"><img alt=""
| + | |
| - | src="https://static.igem.org/mediawiki/2009/1/15/UCDAVIS_RBS1.png"
| + | |
| - | style="border: 0px solid ; width: 69px; height: 63px; background-color: rgb(255, 255, 255);"></a><a
| + | |
| - | href="#SS"><img alt=""
| + | |
| - | src="https://static.igem.org/mediawiki/2009/9/9d/UCDAVIS_SS1.png"
| + | |
| - | style="border: 0px solid ; width: 73px; height: 65px; background-color: rgb(255, 255, 255);"></a><a
| + | |
| - | href="#GFP"><img alt=""
| + | |
| - | src="https://static.igem.org/mediawiki/2009/7/7d/UCDAVIS_GFP.png"
| + | |
| - | style="border: 0px solid ; width: 83px; height: 55px; background-color: rgb(255, 255, 255);"></a><a
| + | |
| - | href="#His"><img alt=""
| + | |
| - | src="https://static.igem.org/mediawiki/2009/c/c0/UCDAVIS_6HIS.png"
| + | |
| - | style="border: 0px solid ; width: 110px; height: 58px; background-color: rgb(255, 255, 255);"></a><a
| + | |
| - | href="#Terminator"><img alt=""
| + | |
| - | src="https://static.igem.org/mediawiki/2009/c/ca/UCDAVIS_STOP.png"
| + | |
| - | style="border: 0px solid ; width: 78px; height: 71px; background-color: rgb(255, 255, 255);"></a></span></small></span></big></big></big><br>
| + | |
| - | <div style="text-align: center;"><big><big><big><span
| + | |
| - | style="font-weight: bold;"><small>b.<span
| + | |
| - | style="text-decoration: underline;"><a href="#LacI"><img alt=""
| + | |
| - | src="https://static.igem.org/mediawiki/2009/4/4e/UCDAVIS_PROMOTER_1.png"
| + | |
| - | style="border: 0px solid ; width: 195px; height: 52px;"></a><a
| + | |
| - | href="#RBS"><img alt=""
| + | |
| - | src="https://static.igem.org/mediawiki/2009/1/15/UCDAVIS_RBS1.png"
| + | |
| - | style="border: 0px solid ; width: 68px; height: 63px;"></a><a
| + | |
| - | href="#SS"><img alt=""
| + | |
| - | src="https://static.igem.org/mediawiki/2009/9/9d/UCDAVIS_SS1.png"
| + | |
| - | style="border: 0px solid ; width: 73px; height: 65px;"></a><a
| + | |
| - | href="#Luciferase"><img alt=""
| + | |
| - | src="https://static.igem.org/mediawiki/2009/9/98/UCDAVIS_Luciferase.png"
| + | |
| - | style="border: 0px solid ; width: 87px; height: 49px;"></a><a
| + | |
| - | href="#His"><img alt=""
| + | |
| - | src="https://static.igem.org/mediawiki/2009/c/c0/UCDAVIS_6HIS.png"
| + | |
| - | style="border: 0px solid ; width: 110px; height: 58px;"></a><a
| + | |
| - | href="#Terminator"><img alt=""
| + | |
| - | src="https://static.igem.org/mediawiki/2009/c/ca/UCDAVIS_STOP.png"
| + | |
| - | style="border: 0px solid ; width: 78px; height: 71px;"></a></span></small></span></big></big></big><big><big><big><span
| + | |
| - | style="font-weight: bold;"><small><span
| + | |
| - | style="text-decoration: underline; background-color: rgb(255, 255, 0);"></span></small></span></big></big></big></div>
| + | |
| - | </div>
| + | |
| - | <div style="text-align: center;"><big><span style="font-style: italic;"></span></big><br>
| + | |
| - | </div>
| + | |
| - | <big><big><big><span
| + | |
| - | style="text-decoration: underline; font-weight: bold;"></span></big></big></big>
| + | |
| - | <hr style="width: 100%; height: 2px;"><b><span
| + | |
| - | style="font-size: 13.5pt; font-family: "Times New Roman","serif";"><a
| + | |
| - | name="INPNC"></a>INPNC:</span></b><span
| + | |
| - | style="font-size: 12pt; font-family: "Times New Roman","serif";">Ice-nucleation
| + | |
| - | protein (INP) from Pseudomonas
| + | |
| - | Syringae was suggested to be used for display of foreign proteins on
| + | |
| - | the
| + | |
| - | surface of <i>E. coli</i>(7).Furthermore, researches have shown that
| + | |
| - | an INP
| + | |
| - | derivative constituting the N-and C-terminal domains can and has been
| + | |
| - | used to
| + | |
| - | display foreign proteins on the surface of <i>E. coli</i>(9). In our
| + | |
| - | project we
| + | |
| - | are intending to harness and make use of this feature by fusing a
| + | |
| - | specific
| + | |
| - | protein to it. <o:p></o:p></span><br>
| + | |
| - | <p class="MsoNormal" style="line-height: normal;"><i><span
| + | |
| - | style="font-size: 12pt; font-family: "Times New Roman","serif";">We
| + | |
| - | have
| + | |
| - | modified this protein to Biobrick standard, Tom Knights Standard.<br>
| + | |
| - | For more information go to: <a
| + | |
| - | href="http://partsregistry.org/wiki/index.php/Part:BBa_K265008">BBa_K265008</a> </span></i><span
| + | |
| - | style="font-size: 12pt; font-family: "Times New Roman","serif";"> <o:p></o:p></span></p>
| + | |
| - | <div class="MsoNormal"
| + | |
| - | style="margin-bottom: 0.0001pt; text-align: center; line-height: normal;"
| + | |
| - | align="center"><span
| + | |
| - | style="font-size: 12pt; font-family: "Times New Roman","serif";">
| + | |
| - | <hr align="center" size="2" width="100%"></span></div>
| + | |
| - | <p class="MsoNormal" style="line-height: normal;"><a name="OmpA"></a><b><span
| + | |
| - | style="font-size: 13pt; font-family: "Times New Roman","serif";">OmpA</span></b><span
| + | |
| - | style="font-size: 18pt; font-family: "Times New Roman","serif";">:</span><span
| + | |
| - | style="font-size: 13pt; font-family: "Times New Roman","serif";"> </span><span
| + | |
| - | style="font-size: 12pt; font-family: "Times New Roman","serif";">OmpA
| + | |
| - | is one of the proteins on the outer
| + | |
| - | membrane of <i>E. coli</i> (13). OmpA has been found to be useful as
| + | |
| - | utilizable
| + | |
| - | fusion part that can fuse our protein to and display on the surface of <i>E.
| + | |
| - | coli</i>. This part has already been documented on the parts registry;
| + | |
| - | however,
| + | |
| - | it has not been tested via fusion with a target protein linked with a
| + | |
| - | cleavable
| + | |
| - | signal sequence. <o:p></o:p></span></p>
| + | |
| - | <p class="MsoNormal" style="line-height: normal;"><i><span
| + | |
| - | style="font-size: 12pt; font-family: "Times New Roman","serif";">We
| + | |
| - | have
| + | |
| - | modified this protein to Biobrick standard, Tom Knights Standard.</span></i><span
| + | |
| - | style="font-size: 12pt; font-family: "Times New Roman","serif";"> <o:p></o:p></span><br>
| + | |
| - | <i><span
| + | |
| - | style="font-size: 12pt; font-family: "Times New Roman","serif";">Note:
| + | |
| - | “It has
| + | |
| - | remained essentially unknown how proteins of E. coli outer membrane are
| + | |
| - | sorted
| + | |
| - | and incorporated into this membrane” (10)</span></i><span
| + | |
| - | style="font-size: 12pt; font-family: "Times New Roman","serif";"> <o:p></o:p></span><br>
| + | |
| - | <i><span
| + | |
| - | style="font-size: 12pt; font-family: "Times New Roman","serif";">For
| + | |
| - | more
| + | |
| - | information go to:<a
| + | |
| - | href="http://partsregistry.org/wiki/index.php/Part:BBa_K103006">
| + | |
| - | BBa_K103006</a></span></i><span
| + | |
| - | style="font-size: 12pt; font-family: "Times New Roman","serif";"> <a
| + | |
| - | href="http://partsregistry.org/wiki/index.php?title=Part:BBa_J36836"><i><span
| + | |
| - | style="color: blue;"></span></i></a><o:p></o:p></span></p>
| + | |
| - | <div class="MsoNormal"
| + | |
| - | style="margin-bottom: 0.0001pt; text-align: center; line-height: normal;"
| + | |
| - | align="center"><span
| + | |
| - | style="font-size: 12pt; font-family: "Times New Roman","serif";">
| + | |
| - | <hr align="center" size="2" width="100%"></span></div>
| + | |
| - | <p class="MsoNormal" style="line-height: normal;"><a name="RBS"></a><b><span
| + | |
| - | style="font-size: 13pt; font-family: "Times New Roman","serif";">RBS</span></b><span
| + | |
| - | style="font-size: 13pt; font-family: "Times New Roman","serif";">:
| + | |
| - | </span><span
| + | |
| - | style="font-size: 12pt; font-family: "Times New Roman","serif";">Ribosome
| + | |
| - | Binding site number 32 (BBa_J61132)
| + | |
| - | from the registry is being used in our secretion system. <o:p></o:p></span><br>
| + | |
| - | <i><span
| + | |
| - | style="font-size: 12pt; font-family: "Times New Roman","serif";">For
| + | |
| - | more
| + | |
| - | information go to:</span></i><span
| + | |
| - | style="font-size: 12pt; font-family: "Times New Roman","serif";"> <big><a
| + | |
| - | href="http://partsregistry.org/wiki/index.php/Part:BBa_J61132"><small><span
| + | |
| - | style="font-style: italic;">BBa_J61132</span></small></a></big><a
| + | |
| - | href="http://partsregistry.org/wiki/index.php/Part:BBa_J61132"><i><span
| + | |
| - | style="color: blue;"></span></i></a><o:p></o:p></span></p>
| + | |
| - | <div class="MsoNormal"
| + | |
| - | style="margin-bottom: 0.0001pt; text-align: center; line-height: normal;"
| + | |
| - | align="center"><span
| + | |
| - | style="font-size: 12pt; font-family: "Times New Roman","serif";">
| + | |
| - | <hr align="center" size="2" width="100%"></span></div>
| + | |
| - | <p class="MsoNormal" style="line-height: normal;"><a name="Terminator"></a><b><span
| + | |
| - | style="font-size: 13pt; font-family: "Times New Roman","serif";">Terminator</span></b><span
| + | |
| - | style="font-size: 13pt; font-family: "Times New Roman","serif";">: </span><span
| + | |
| - | style="font-size: 12pt; font-family: "Times New Roman","serif";">We
| + | |
| - | are using BBa_B0015, a double terminator, as
| + | |
| - | our terminator in both our secretion and pH system.<o:p></o:p></span><br>
| + | |
| - | <i><span
| + | |
| - | style="font-size: 12pt; font-family: "Times New Roman","serif";">For
| + | |
| - | more
| + | |
| - | information go to:</span></i><span
| + | |
| - | style="font-size: 12pt; font-family: "Times New Roman","serif";"> <a
| + | |
| - | href="http://partsregistry.org/wiki/index.php?title=Part:BBa_B0015"><span
| + | |
| - | style="font-style: italic;">BBa_B0015</span></a> <a
| + | |
| - | href="http://partsregistry.org/wiki/index.php?title=Part:BBa_B0015"><i><span
| + | |
| - | style="color: blue;"></span></i></a><o:p></o:p></span></p>
| + | |
| - | <div class="MsoNormal"
| + | |
| - | style="margin-bottom: 0.0001pt; text-align: center; line-height: normal;"
| + | |
| - | align="center"><span
| + | |
| - | style="font-size: 12pt; font-family: "Times New Roman","serif";">
| + | |
| - | <hr align="center" size="2" width="100%"></span></div>
| + | |
| - | <p class="MsoNormal" style="line-height: normal;"><a name="GFP"></a><b><span
| + | |
| - | style="font-size: 13pt; font-family: "Times New Roman","serif";">GFP</span></b><span
| + | |
| - | style="font-size: 13pt; font-family: "Times New Roman","serif";">: </span><span
| + | |
| - | style="font-size: 12pt; font-family: "Times New Roman","serif";">We
| + | |
| - | are using Green Fluorescent Protein as a
| + | |
| - | reporter that also serves as a small protein in testing our secretion
| + | |
| - | system.<br>
| + | |
| - | <span style="font-style: italic;">For more informaiton go to: </span><a
| + | |
| - | style="font-style: italic;"
| + | |
| - | href="http://partsregistry.org/wiki/index.php/Part:BBa_K265003">BBa_K265003</a><span
| + | |
| - | style="font-style: italic;"> </span><o:p></o:p></span></p>
| + | |
| - | <div class="MsoNormal"
| + | |
| - | style="margin-bottom: 0.0001pt; text-align: center; line-height: normal;"
| + | |
| - | align="center"><span
| + | |
| - | style="font-size: 12pt; font-family: "Times New Roman","serif";">
| + | |
| - | <hr align="center" size="2" width="100%"></span></div>
| + | |
| - | <p class="MsoNormal" style="line-height: normal;"><a name="Luciferase"></a><b><span
| + | |
| - | style="font-size: 13pt; font-family: "Times New Roman","serif";">Luciferase</span></b><span
| + | |
| - | style="font-size: 13pt; font-family: "Times New Roman","serif";">: </span><span
| + | |
| - | style="font-size: 12pt; font-family: "Times New Roman","serif";">Luciferase
| + | |
| - | is a firefly protein that also
| + | |
| - | fluoresces, so it serves as a reporter as well as a testable large
| + | |
| - | protein.<br>
| + | |
| - | <span style="font-style: italic;">For more information go to: <a
| + | |
| - | href="http://partsregistry.org/wiki/index.php/Part:BBa_I712019">BBa_1712019</a></span>
| + | |
| - | <o:p></o:p></span></p>
| + | |
| - | <div class="MsoNormal"
| + | |
| - | style="margin-bottom: 0.0001pt; text-align: center; line-height: normal;"
| + | |
| - | align="center"><span
| + | |
| - | style="font-size: 12pt; font-family: "Times New Roman","serif";">
| + | |
| - | <hr align="center" size="2" width="100%"></span></div>
| + | |
| - | <p class="MsoNormal" style="line-height: normal;"><a name="LacI"></a><b><span
| + | |
| - | style="font-size: 13pt; font-family: "Times New Roman","serif";">LacI</span></b><span
| + | |
| - | style="font-size: 13pt; font-family: "Times New Roman","serif";">: </span><span
| + | |
| - | style="font-size: 12pt; font-family: "Times New Roman","serif";">One
| + | |
| - | inducible Promoter which was found in the
| + | |
| - | part registry.<br>
| + | |
| - | </span><i><span
| + | |
| - | style="font-size: 13pt; font-family: "Times New Roman","serif";"></span></i><i><span
| + | |
| - | style="font-size: 12pt; font-family: "Times New Roman","serif";">For
| + | |
| - | more information go to:<a
| + | |
| - | href="http://partsregistry.org/wiki/index.php/Part:BBa_R0010">
| + | |
| - | BBa_R0010</a><br>
| + | |
| - | </span></i><span
| + | |
| - | style="font-size: 12pt; font-family: "Times New Roman","serif";"><a
| + | |
| - | href="http://partsregistry.org/wiki/index.php?title=Part:BBa_R0010"><i><span
| + | |
| - | style="color: blue;"></span></i></a><o:p></o:p></span></p>
| + | |
| - | <div class="MsoNormal"
| + | |
| - | style="margin-bottom: 0.0001pt; text-align: center; line-height: normal;"
| + | |
| - | align="center"><span
| + | |
| - | style="font-size: 12pt; font-family: "Times New Roman","serif";">
| + | |
| - | <hr align="center" size="2" width="100%"></span></div>
| + | |
| - | <p class="MsoNormal" style="line-height: normal;"><a name="SS"></a><b><span
| + | |
| - | style="font-size: 13pt; font-family: "Times New Roman","serif";">SS</span></b><span
| + | |
| - | style="font-size: 13pt; font-family: "Times New Roman","serif";">:</span><span
| + | |
| - | style="font-size: 12pt; font-family: "Times New Roman","serif";">This
| + | |
| - | signal sequence, when placed between
| + | |
| - | INPNC, contains a cleavable site that allows the target fusion protein
| + | |
| - | to
| + | |
| - | ‘secrete’ from INPNC. We will do the same with OmpA. <o:p></o:p></span><br>
| + | |
| - | <i><span
| + | |
| - | style="font-size: 12pt; font-family: "Times New Roman","serif";">We
| + | |
| - | have
| + | |
| - | modified this protein to Biobrick standard, Tom Knights Standard.<br>
| + | |
| - | For more infromation go to :<a
| + | |
| - | href="http://partsregistry.org/wiki/index.php/Part:BBa_K265002">BBa_K265002</a></span></i><span
| + | |
| - | style="font-size: 12pt; font-family: "Times New Roman","serif";"> <o:p></o:p></span></p>
| + | |
| - | <div class="MsoNormal"
| + | |
| - | style="margin-bottom: 0.0001pt; text-align: center; line-height: normal;"
| + | |
| - | align="center"><span
| + | |
| - | style="font-size: 12pt; font-family: "Times New Roman","serif";">
| + | |
| - | <hr align="center" size="2" width="100%"></span></div>
| + | |
| - | <a name="His"></a><b><span
| + | |
| - | style="font-size: 13pt; font-family: "Times New Roman","serif";">6-His
| + | |
| - | Tag</span></b><span
| + | |
| - | style="font-size: 13pt; font-family: "Times New Roman","serif";">:</span><span
| + | |
| - | style="font-size: 12pt; font-family: "Times New Roman","serif";">The
| + | |
| - | 6-Histidine Tag serves as a tag for Western
| + | |
| - | Blotting if our fluorescent reporters are not expressed as highly as we
| + | |
| - | would
| + | |
| - | like. <o:p></o:p></span><br>
| + | |
| - | <small><i><span
| + | |
| - | style="font-size: 12pt; font-family: "Times New Roman","serif";">Note:
| + | |
| - | We are
| + | |
| - | using this tag, just in case if the GFP or Luciferase does not work
| + | |
| - | under a
| + | |
| - | plate reader.</span></i></small>
| + | |
| - | <hr style="width: 100%; height: 2px;"><span
| + | |
| - | style="font-size: 12pt; font-family: "Times New Roman","serif";"></span><b><i><span
| + | |
| - | style="font-size: 12pt; line-height: 115%; font-family: "Times New Roman","serif";"></span></i></b><big><big><big><span
| + | |
| - | style="text-decoration: underline; font-weight: bold;"></span></big></big></big>
| + | |
| - | </body>
| + | |
| - | </html>
| + | |
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