Duke University/22 June 2009

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(Difference between revisions)
(New page: Did PCR with fragments 1 and 2 and different conditions (decreased annealing and extension times and number of cycles)<br> Ran gel to check PCR)
 
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Did PCR with fragments 1 and 2 and different conditions (decreased annealing and extension times and number of cycles)<br>
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[[Team:Duke | Return]]
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Ran gel to check PCR
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==June 22, 2009==
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<pre>
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LB Agar Plates with Chlorophenicol (CAM), anhydrotetracycline, Nile Red (NR)
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stock plate
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CAM 50 mg/ul 20 ug/ml
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anhyd 2 mg/ml 150 ng/ml
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NR 0.25 mg/ml 0.5 ug/ml
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</pre>
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*E: PCR with fragments 1 and 2 and different conditions (decreased annealing and extension times and number of cycles), Gel to check PCR
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<pre>
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2x Phusion mix 12.5 ul
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pASKphaA 4.76 ul
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phaB 0.60 ul
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H2O 7.14 ul
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25 ul
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98°C 30s 1x
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98°C 10s
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70-55°C 10s
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72°C 1m 10s 20x
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72°C 5m
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4°C infinity 1x</pre>
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*R: No band/smear
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*C: Change conditions

Latest revision as of 16:48, 21 October 2009

Return

June 22, 2009

LB Agar Plates with Chlorophenicol (CAM), anhydrotetracycline, Nile Red (NR)
stock plate
CAM 50 mg/ul 20 ug/ml
anhyd 2 mg/ml 150 ng/ml
NR 0.25 mg/ml 0.5 ug/ml
  • E: PCR with fragments 1 and 2 and different conditions (decreased annealing and extension times and number of cycles), Gel to check PCR 
2x Phusion mix 12.5 ul 
pASKphaA 4.76 ul
phaB 0.60 ul
H2O 7.14 ul 
25 ul

98°C 30s 1x
98°C 10s
70-55°C 10s 
72°C 1m 10s 20x
72°C 5m
4°C infinity 1x
  • R: No band/smear
  • C: Change conditions
Retrieved from "http://2009.igem.org/Duke_University/22_June_2009"