"
Team:Bologna
From 2009.igem.org
| Line 15: | Line 15: | ||
<html> | <html> | ||
<font face="Calibri" font size="4" color="#000000"> | <font face="Calibri" font size="4" color="#000000"> | ||
| - | The project aims to design a new device to control the synthesis of a protein in | + | The project aims to design a new device to control the synthesis of a protein in <i>Escherichia coli</i> regardless of the protein to be controlled. This "general-purpose" standard device acts on translation to allow a faster silencing of protein expression if compared to standard regulated promoters. We named this device <b>T-Rex</b> (<b>T</b>rans <b>R</b>epressor of <b>Ex</b>pression). |
</font></html> | </font></html> | ||
| Line 27: | Line 27: | ||
<font face="Calibri" font size="4" color="#000000"> | <font face="Calibri" font size="4" color="#000000"> | ||
The device consists of two new BioBricks: | The device consists of two new BioBricks: | ||
| - | + | <br> | |
<ul> | <ul> | ||
<li><font color="#000080"><b>CIS-repressing</b></font>, to be assembled upstream of the target coding sequence. | <li><font color="#000080"><b>CIS-repressing</b></font>, to be assembled upstream of the target coding sequence. | ||
</ul> | </ul> | ||
| - | |||
<ul> | <ul> | ||
<li><font color="#000080"><b>TRANS-repressor</b></font>, complementary to the CIS-repressing and placed under the control of a different promoter. | <li><font color="#000080"><b>TRANS-repressor</b></font>, complementary to the CIS-repressing and placed under the control of a different promoter. | ||
</ul></html> | </ul></html> | ||
<br> | <br> | ||
| - | CIS-repressing and TRANS-repressor were | + | CIS-repressing and TRANS-repressor sequences were seeked by [[Team:Bologna/Software#1|BASER]] software. |
<br><br> | <br><br> | ||
| - | Transcription of the target gene produces a mRNA strand, starting with the Cis element, which is translated into proteins by ribosome (see Fig 1, right panel). Trans’ promoter induction produces a transcript that binds with the Cis part. The <b>RNA duplex</b> prevents ribosome from binding to RBS, <b>repressing protein synthesis</b>. Thus, the TRANS-repressor amount regulates the gene mRNA translation rate (see Fig 1, left panel) | + | Transcription of the target gene produces a mRNA strand, starting with the Cis element, which is translated into proteins by ribosome (<i>see Fig. 1, right panel</i>). Trans’ promoter induction produces a transcript that binds with the Cis part. The <b>RNA duplex</b> prevents ribosome from binding to RBS, <b>repressing protein synthesis</b>. Thus, the TRANS-repressor amount regulates the gene mRNA translation rate (<i>see Fig. 1, left panel</i>) |
<br><br> | <br><br> | ||
| - | [[Image:project3b.png|center|950px|thumb|<center> | + | [[Image:project3b.png|center|950px|thumb|<center>Figure 1 - T-Rex device</center>]] |
<br><br> | <br><br> | ||
| Line 52: | Line 51: | ||
</html> | </html> | ||
<br><br> | <br><br> | ||
| - | [[Image:circuit2.jpg|center|900px|thumb|<center> | + | [[Image:circuit2.jpg|center|900px|thumb|<center>Figure 2 - Genetic Circuit</center>]] |
<font face="Calibri" font size="4" color="#000000"> | <font face="Calibri" font size="4" color="#000000"> | ||
<br><br> | <br><br> | ||
Revision as of 17:12, 21 October 2009
| HOME | TEAM | PROJECT | SOFTWARE | MODELING | WET LAB | PARTS | HUMAN PRACTICE | JUDGING CRITERIA |
|---|
Project Summary
Which is our idea?
The project aims to design a new device to control the synthesis of a protein in Escherichia coli regardless of the protein to be controlled. This "general-purpose" standard device acts on translation to allow a faster silencing of protein expression if compared to standard regulated promoters. We named this device T-Rex (Trans Repressor of Expression).
How does T-Rex work?
The device consists of two new BioBricks:
CIS-repressing and TRANS-repressor sequences were seeked by BASER software.
Transcription of the target gene produces a mRNA strand, starting with the Cis element, which is translated into proteins by ribosome (see Fig. 1, right panel). Trans’ promoter induction produces a transcript that binds with the Cis part. The RNA duplex prevents ribosome from binding to RBS, repressing protein synthesis. Thus, the TRANS-repressor amount regulates the gene mRNA translation rate (see Fig. 1, left panel)
How can we test the device?
In order to test and characterize our T-REX device, we developed the following genetic circuit (Fig 2):
The T-REX device is proposed as a universal and fast switch in synthetic gene circuits.
More details about our work in the Project section.
Acknowledgements
- [http://www.unibo.it/Portale/default.htm University of Bologna]
- [http://serinar.criad.unibo.it Ser.In.Ar. Cesena]
- Cultural Association San Sebastiano
