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Team:Groningen/Parts
From 2009.igem.org
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Revision as of 18:44, 21 October 2009
[http://2009.igem.org/Team:Groningen http://2009.igem.org/wiki/images/f/f1/Igemhomelogo.png]
| Biobricks
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[http://2009.igem.org/Team:Groningen/Parts/Submitted_Parts http://2009.igem.org/wiki/images/d/dd/Next.JPG]
Parts
TODOHere we need to give some genereal information about our parts.
- For a comprehensive list of all the parts we used, have a look at our [http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2009&group=Groningen parts registry].
- Here you can find an informative overview of our submitted parts.
- Experience we had with parts from the registry can be found under used parts.
Note
If you choose to include a Parts Submitted to the Registry page, please list your parts here. This is not necessary but it may be a nice list to keep track of.
- http://partsregistry.org/wiki/images/d/db/Buttonaddpart.gif
[http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2009&group=Groningen Our sandbox]
Part List -80
| Component | Description | Part or Accession # | Base Pairs (bp) | Plasmid (backbone) | Resistance | Well | Quality control |
| GVP | Gas Vesicle Proteins | 6064 | Ampicillin/Gentamycin | plate 3, pos.1 | ? | ||
| GVP (2nd batch) | Gas Vesicle Proteins | 6064 | Ampicillin/Gentamycin | plate 3, pos.2 | ? | ||
| pHigh in low copy nr vector | High consitutive promoter | 35 | Kanamycin | Plate 3, pos. 4 | PCR(?), restriction(+) | ||
| pMed in low copy nr vector | Medium consitutive promoter | 35 | Kanamycin | Plate 3, pos. 5 | PCR(?), restriction(+) | ||
| pLow in low copy nr vector | Low consitutive promoter | 35 | Kanamycin | Plate 3, pos. 6 | PCR(?), restriction(+) | ||
| pHigh in high copy nr vector | High consitutive promoter | 35 | Ampicillin/Chloramphenicol | Plate 3, pos. 7 | PCR(?), restriction(+) | ||
| pMed in high copy nr vector | Medium consitutive promoter | 35 | Ampicillin/Chloramphenicol | Plate 3, pos. 8 | PCR(?), restriction(+) | ||
| pLow in high copy nr vector | High consitutive promoter | 35 | Ampicillin/Chloramphenicol | Plate 3, pos. 9 | PCR(?), restriction(+) | ||
| Promotor (low expression) | Constitutive promotor | 35 | Ampicillin | plate 3, pos.10 | No | ||
| Promotor (medium expression) | Constitutive promotor | 35 | Ampicillin | plate 3, pos.11 | No | ||
| Promotor (high expression) | Constitutive promotor | 35 | Ampicillin | plate 3, pos.12 | No | ||
| Inducible promoter | Lactose inducible promoter nr1 | 200 | Ampicillin | Plate 3, pos. 14 | PCR(?), restriction(+) | ||
| Inducible promoter | Lactose inducible promoter nr2 | 200 | Ampicillin | Plate 3, pos. 15 | PCR(?), restriction(+) | ||
| RBS | Ribosomal Binding Site | 12 | Ampicillin | plate 3, pos.19 | No | ||
| Terminator | Double terminator consisting of and | ~90 | Ampicillin/Kanamycin | plate 3, pos.20 | No | ||
| Const. Promoter + GVP | Low constitutive promoter with GVP cluster | + | ~6100 | Ampicillin/Chloramphenicol | plate 3, pos.21 | EcoRI/PstI + Sequenced Okay | |
| Const. Promoter + GVP (no.2) | Low constitutive promoter with GVP cluster | + | ~6100 | Ampicillin/Chloramphenicol | plate 3, pos.22 | EcoRI/PstI + Sequenced Okay | |
| Const. Promoter + GVP | Medium constitutive promoter with GVP cluster | + | ~6100 | Ampicillin/Chloramphenicol | plate 3, pos.23 | EcoRI/PstI + Sequenced Okay | |
| Const. Promoter + GVP (no.2) | Medium constitutive promoter with GVP cluster | + | ~6100 | Ampicillin/Chloramphenicol | plate 3, pos.24 | EcoRI/PstI + Sequenced Okay | |
| HmtA (1.1) | HmtA-His in pBAD(a) | None (to become ) | 2004 | [http://ecoliwiki.net/colipedia/index.php/pBAD/myc-His_A pBAD] | Ampicillin | plate 3, pos.28 | Restriction(+) |
| HmtA (2.1) | HmtA-His in pBAD(a) | None (to become ) | 2004 | [http://ecoliwiki.net/colipedia/index.php/pBAD/myc-His_A pBAD] | Ampicillin | plate 3, pos.29 | Restriction(+) |
| RBS MBPArsR | + | None (to become ) | ~1600 | Ampicillin | plate 3, pos.30,31,32 | Restriction(+)PCR | |
| pLac-fMT + Ars-RFP | pArs testing | (without the backbone) | ~1400 | Ampicillin | plate 3, pos. 33,34,35 | Quality control | |
| CueO- + GVP in J61035 | CueO promoter without RBS in front of the GVP cluster | None | 6149 | (without the part between EcoRI and XbaI) | Ampicillin | plate 3, pos.37 | Restriction(+) + Sequenced Okay |
| ZntR- + GVP in J61035 | ZntR promoter without RBS in front of the GVP cluster | None | ~6150 | (without the part between EcoRI and XbaI) | Ampicillin | plate 3, pos.38 | Restriction(+) + Sequenced (3 mutations) |
| ArsR- + GVP in J61035 | ArsR promoter without RBS in front of the GVP cluster | None | ~6150 | (without the part between EcoRI and XbaI) | Ampicillin | plate 3, pos.39 | Restriction(+) + Sequenced Okay |
| ArsR- in J61002 | pArsR (promoter without RBS) | 71 | (without the part between EcoRI and SpeI) | Ampicillin | plate 3, pos.40 | EcoRI/SpeI(+), EcoRI/PstI(+), Sequenced Okay | |
| ZntR- in J61002 | pZntR (promoter without RBS) | 65 | (without the part between EcoRI and SpeI) | Ampicillin | plate 3, pos.41 | EcoRI/SpeI(+), EcoRI/PstI(+), Sequenced (3 mutations) | |
| CueO- in J61002 | pCueO (promoter without RBS) | 43 | (without the part between EcoRI and SpeI) | Ampicillin | plate 3, pos.42 | EcoRI/SpeI(+), EcoRI/PstI(+), Sequenced Okay | |
| ZntR+RBS in J61002 | pZntR (promoter with own RBS) | 91 | (without the part between EcoRI and SpeI) | Ampicillin | plate 3, pos.43 | EcoRI/SpeI(+), EcoRI/PstI(+), Sequenced Okay | |
| ArsR+RBS in J61002 | pArsR (promoter with own RBS) | 81 | (without the part between EcoRI and SpeI) | Ampicillin | plate 3, pos.44 | EcoRI/SpeI(+), EcoRI/PstI(+), Sequenced Okay | |
| CueO+RBS in J61002 | pCueO (promoter with own RBS) | 71 | (without the part between EcoRI and SpeI) | Ampicillin | plate 3, pos.45 | EcoRI/SpeI(+), EcoRI/PstI(+), Sequenced Okay | |
| SmtA-GST in pGEX-3x | SmtA-GST fusion | no biobrick primers available | 1400 | [http://www.ecoliwiki.net/colipedia/index.php/pGEX-3X pGEX-3x] | Ampicillin | plate 3, pos. 46 | PCR, restriction |
| SmtA in pET29a | SmtA | 900 | [http://www.merckbiosciences.co.uk/product/69871 pET29a] | Kanamycin | plate 3, pos. 47 | PCR, restriction | |
| MymT in pGB68 | MymT | 215 | [http://www.neb.com/nebecomm/products/productN6707.asp pTXB1] (NEB) | Ampicillin | plate 3, pos. 48 | PCR, restriction | |
| GlpF and fMT in pMAL-MT | GlpF and fMT on a synthetic operon in the vector | and | fMT = 200, GlpF = 900? | [http://www.neb.com/nebecomm/products/productN8076.asp pMALc2x] (NEB) | Ampicillin | plate 3, pos. 49 | PCR, restriction |
| MBP-ArsR | MBP-ArsR Fusion protein | 15180 | Ampicillin/chloramphenicol | plate 3, pos. 53 | Restriction analysis & PCR | ||
| pNL29 | GVP Gene cluster | 6000 | [http://www.fermentas.com/techinfo/nucleicacids/mappbluescriptiiskks.htm pBluescriptIIKS] | Ampicillin | plate 3, pos. 54 | Buoyancy phenotype | |
| pLac-fMT | Metallothionein with inducible promotor | 430 | Ampicillin/chloramphenicol | plate 3, pos. 55 | Quality control | ||
| pLow-fMT #4 | Metallothionein with low constitutive promotor | 265 | Ampicillin/chloramphenicol | plate 3, pos. 56 | Quality control | ||
| pLow-fMT #3 | Metallothionein with low constitutive promotor | 265 | Ampicillin/chloramphenicol | plate 3, pos. 57 | Quality control | ||
| SmtA | Metallothionein | 678 | Ampicillin/chloramphenicol | plate 3, pos. 58 | Quality control | ||
| fMT #6 | Metallothionein for As3+ | 222 | Ampicillin/chloramphenicol | plate 3, pos. 59 | Sequence 2 deletions between EcoRI and XbaI sites | ||
| fMT #4 | Metallothionein for As3+ | 222 | Ampicillin/chloramphenicol | plate 3, pos. 60 | Sequence Okay! | ||
| pZntR-GVP | Metal promoter | Base Pairs (bp) | Kanamycin | plate 3, pos. 69 | Quality control | ||
| pCueO-GVP | Metal promoter | Base Pairs (bp) | Kanamycin | plate 3, pos. 70 | Quality control | ||
| pLacI-GVP no.1 | Inducible promoter | Base Pairs (bp) | Ampicillin | plate 3, pos. 71 | Quality control | ||
| pLacI-GVP no.2 | Inducible promoter | Base Pairs (bp) | Ampicillin | plate 3, pos. 72 | Quality control | ||
| High consitutive promoter(with RFP) | Constitutive promoter | Base Pairs (bp) | Ampicillin/chloramphenicol | plate 3, pos. 75 | Quality control | ||
| Medium consitutive promoter(with RFP) | Constitutive promoter | Base Pairs (bp) | Ampicillin/chloramphenicol | plate 3, pos. 76 | Quality control | ||
| Low consitutive promoter(with RFP) | Constitutive promoter | Base Pairs (bp) | Ampicillin/chloramphenicol | plate 3, pos. 77 | Quality control | ||
| High consitutive promoter(with RFP) | Constitutive promoter | Base Pairs (bp) | Kanamycin | plate 3, pos. 78 | Quality control | ||
| Medium consitutive promoter(with RFP) | Constitutive promoter | Base Pairs (bp) | Kanamycin | plate 3, pos. 79 | Quality control | ||
| Low consitutive promoter(with RFP) | Constitutive promoter | Base Pairs (bp) | Kanamycin | plate 3, pos. 80 | Quality control | ||
| Reference constitutive promoter) | Constitutive promoter | ? | Base Pairs (bp) | ? | ? | plate 3, pos. 81 | Quality control |
| ArsR-GvP | Ars promotor + GvP gas vesicle cluster | ? | ? | Kanamycin | plate 3, pos. 64 | ? |
